Incidental Mutation 'FR4304:Cfap74'
ID 510985
Institutional Source Beutler Lab
Gene Symbol Cfap74
Ensembl Gene ENSMUSG00000078490
Gene Name cilia and flagella associated protein 74
Synonyms 2010015L04Rik
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # FR4304 ()
Quality Score 221.999
Status Not validated
Chromosome 4
Chromosomal Location 155493647-155551280 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 155500217 bp (GRCm39)
Zygosity Homozygous
Amino Acid Change Aspartic acid to Glycine at position 21 (D21G)
Ref Sequence ENSEMBL: ENSMUSP00000131899 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000123952] [ENSMUST00000151083]
AlphaFold Q3UY96
Predicted Effect noncoding transcript
Transcript: ENSMUST00000094408
SMART Domains Protein: ENSMUSP00000091975
Gene: ENSMUSG00000042233

DomainStartEndE-ValueType
low complexity region 44 59 N/A INTRINSIC
coiled coil region 134 162 N/A INTRINSIC
coiled coil region 333 378 N/A INTRINSIC
Predicted Effect possibly damaging
Transcript: ENSMUST00000123952
AA Change: D21G

PolyPhen 2 Score 0.934 (Sensitivity: 0.80; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000131899
Gene: ENSMUSG00000078490
AA Change: D21G

DomainStartEndE-ValueType
low complexity region 44 59 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000129481
Predicted Effect noncoding transcript
Transcript: ENSMUST00000145520
Predicted Effect possibly damaging
Transcript: ENSMUST00000151083
AA Change: D21G

PolyPhen 2 Score 0.869 (Sensitivity: 0.83; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000123626
Gene: ENSMUSG00000078490
AA Change: D21G

DomainStartEndE-ValueType
low complexity region 44 59 N/A INTRINSIC
coiled coil region 134 162 N/A INTRINSIC
coiled coil region 333 378 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000152780
Predicted Effect noncoding transcript
Transcript: ENSMUST00000166921
Predicted Effect noncoding transcript
Transcript: ENSMUST00000166858
Coding Region Coverage
  • 1x: 99.8%
  • 3x: 99.6%
  • 10x: 98.6%
  • 20x: 96.4%
Validation Efficiency
Allele List at MGI

All alleles(7) : Targeted(2) Gene trapped(5)

Other mutations in this stock
Total: 137 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700001K19Rik CTT CTTTTT 12: 110,634,883 (GRCm39) probably benign Homo
1700001K19Rik TTC TTCGTC 12: 110,634,884 (GRCm39) probably benign Homo
4930433I11Rik ACCTC AC 7: 40,642,480 (GRCm39) probably benign Het
4930447C04Rik AAGT A 12: 72,928,061 (GRCm39) probably benign Homo
Acbd4 CAG CAGACTAG 11: 102,994,931 (GRCm39) probably null Homo
Ahdc1 CT CTCTT 4: 132,790,070 (GRCm39) probably benign Homo
Alpk3 TCT TCTGCT 7: 80,727,510 (GRCm39) probably benign Het
Anapc4 C T 5: 53,021,868 (GRCm39) T650M probably damaging Homo
Ankhd1 GGCGGC GGCGGCTGCGGC 18: 36,693,977 (GRCm39) probably benign Het
Ankrd35 TAGC TAGCAGC 3: 96,591,163 (GRCm39) probably benign Homo
Apc GCCAATAAA GCCAATAAAACCAATAAA 18: 34,415,050 (GRCm39) probably benign Het
Apol6 TTGT TTGTCTGT 15: 76,935,636 (GRCm39) probably null Het
Arhgap30 TGGCCC TGGCCCTGGCCCAGGCCTTGGCCCCGGCCC 1: 171,232,736 (GRCm39) probably benign Het
Arpc1b GCC GCCTGTCC 5: 145,063,601 (GRCm39) probably null Het
Blm CT CTACGT 7: 80,113,521 (GRCm39) probably null Homo
Blm TCCTCCTCCTCC TCCTCCTCCTCCACCTCCTCCTCC 7: 80,162,667 (GRCm39) probably benign Het
Btnl10 GA GAATA 11: 58,814,756 (GRCm39) probably benign Homo
Cacna1f AGG AGGCGG X: 7,486,300 (GRCm39) probably benign Het
Calhm3 CG CGG 19: 47,140,335 (GRCm39) probably null Homo
Catsper2 C CTTTTACTTTTTA 2: 121,228,023 (GRCm39) probably null Homo
Catsper2 CAT CATTAT 2: 121,228,263 (GRCm39) probably benign Het
Ccdc121 GAGAAG GAG 5: 31,644,717 (GRCm39) probably benign Homo
Ccdc15 AC ACTTTCC 9: 37,226,453 (GRCm39) probably null Het
Ccdc162 T C 10: 41,432,117 (GRCm39) D1792G possibly damaging Het
Ccdc170 CCA CCATCA 10: 4,511,021 (GRCm39) probably benign Het
Ccdc73 TAAG T 2: 104,822,185 (GRCm39) probably benign Homo
Ccdc85c GCC GCCCCC 12: 108,240,871 (GRCm39) probably benign Het
Cd22 C T 7: 30,577,507 (GRCm39) R2H possibly damaging Het
Cd80 AGA AGAGGA 16: 38,306,677 (GRCm39) probably benign Homo
Cep89 GACT G 7: 35,109,066 (GRCm39) probably benign Het
Cgref1 T TCTA 5: 31,091,124 (GRCm39) probably benign Homo
Chd4 GCC GCCACTCCC 6: 125,099,107 (GRCm39) probably benign Het
Cnpy3 TCC TCCCCC 17: 47,047,669 (GRCm39) probably benign Het
Cnpy3 TCC TCCACC 17: 47,047,672 (GRCm39) probably benign Het
Cntnap1 AGCCCC AGCCCCCGCCCC 11: 101,080,407 (GRCm39) probably benign Het
Cntnap1 CCCCAG CCCCAGACCCAG 11: 101,080,415 (GRCm39) probably benign Het
Col2a1 C A 15: 97,886,862 (GRCm39) probably null Homo
Cpeb4 T TGA 11: 31,877,638 (GRCm39) probably benign Homo
Cpne1 AGA AGAGAGA 2: 155,913,945 (GRCm39) probably null Homo
Cttnbp2 ATTGCTG ATTGCTGTTGCTG 6: 18,367,457 (GRCm39) probably benign Het
Dhx37 CTGG C 5: 125,504,594 (GRCm39) probably benign Het
Dhx8 CGAGAC CGAGACGGAGAC 11: 101,629,014 (GRCm39) probably benign Homo
Dnaaf9 TCC TCCCCC 2: 130,612,668 (GRCm39) probably benign Het
Dnah12 G T 14: 26,571,342 (GRCm39) G2817V probably damaging Homo
Dst C A 1: 34,240,045 (GRCm39) S1798Y probably damaging Het
Eif3a TA TATTTCA 19: 60,763,728 (GRCm39) probably benign Homo
Ermn TTC TTCCTC 2: 57,938,090 (GRCm39) probably benign Het
Ermn CTT CTTGTT 2: 57,938,098 (GRCm39) probably benign Het
Fbxo43 TGTGCC TGTGCCAGTGCC 15: 36,152,243 (GRCm39) probably benign Het
Fbxo43 GCCTGT GCCTGTCCCTGT 15: 36,152,246 (GRCm39) probably benign Het
Fbxo43 GCCTGT GCCTGTTCCTGT 15: 36,152,240 (GRCm39) probably benign Het
Fmn1 TCC TCCTCCACC 2: 113,356,128 (GRCm39) probably benign Homo
Fmn1 TCCTCC TCCTCCCCCTCC 2: 113,356,119 (GRCm39) probably benign Het
Foxd3 GGACCCTACGGCCG GG 4: 99,545,633 (GRCm39) probably benign Homo
Frmpd2 G T 14: 33,232,978 (GRCm39) L399F probably damaging Homo
Gabre ACTCCGGCTCCGGCTCAGGCTCAGGCTCCGGCTCAGGCTCCGGCTCCGGCTCCGGCTC ACTCCGGCTCCGGCTCCGGCTCAGGCTCAGGCTCCGGCTCAGGCTCCGGCTCCGGCTCCGGCTC X: 71,313,648 (GRCm39) probably benign Homo
Gbp2b A G 3: 142,309,413 (GRCm39) I175V probably benign Het
Gm4340 CAG CAGAAG 10: 104,031,933 (GRCm39) probably benign Het
Gm4340 AGC AGCGGC 10: 104,031,943 (GRCm39) probably benign Het
Gm5114 T C 7: 39,060,529 (GRCm39) R107G probably benign Het
Gm5114 A C 7: 39,060,530 (GRCm39) H106Q probably benign Het
H1f6 GAGAA GA 13: 23,879,903 (GRCm39) probably benign Homo
H2-Q4 G A 17: 35,599,381 (GRCm39) D155N probably damaging Het
H2-T10 TGTTTCCCACTG T 17: 36,431,173 (GRCm39) probably null Het
Ifi203 C T 1: 173,755,894 (GRCm39) probably benign Het
Ifi208 ATGGTG ATG 1: 173,505,264 (GRCm39) probably benign Homo
Ighv5-9 C T 12: 113,625,497 (GRCm39) S82N probably benign Homo
Il17rd CGG CGGTGG 14: 26,804,637 (GRCm39) probably benign Het
Il2 AGTGG AGTGGGGCTTGAGGTGG 3: 37,179,975 (GRCm39) probably benign Het
Ipo9 TCC TCCGCC 1: 135,314,013 (GRCm39) probably benign Het
Ipo9 CCT CCTACT 1: 135,314,017 (GRCm39) probably null Het
Isg20l2 AAG AAGCAG 3: 87,839,019 (GRCm39) probably benign Homo
Kmt2b TCCTCC TCCTCCCCCTCC 7: 30,285,788 (GRCm39) probably benign Het
Kmt2c TGCTGCTG TGCTGCTGCTGCTG 5: 25,520,764 (GRCm39) probably benign Homo
Krt10 CGCC CGCCGCC 11: 99,277,025 (GRCm39) probably benign Het
Krt10 CCTCCT CCTCCTACTCCT 11: 99,280,100 (GRCm39) probably benign Het
Las1l GAG GAGCAG X: 94,984,426 (GRCm39) probably benign Het
Las1l AGG AGGCGG X: 94,984,427 (GRCm39) probably benign Het
Lkaaear1 GCTCCAGCTCCAGCTCCAGCTCCA GCTCCAGCTCCATCTCCAGCTCCAGCTCCAGCTCCA 2: 181,339,372 (GRCm39) probably benign Het
Lrch1 A T 14: 75,057,005 (GRCm39) C241S possibly damaging Het
Lrit3 G GCTT 3: 129,582,468 (GRCm39) probably benign Het
Maml2 GCAGCAGCAACAGCAGCA GCAGCAGCA 9: 13,532,755 (GRCm39) probably benign Homo
Mast4 T TTTC 13: 102,871,370 (GRCm39) probably benign Het
Med12l AGC AGCGGC 3: 59,183,403 (GRCm39) probably benign Het
Muc21 T G 17: 35,933,013 (GRCm39) probably benign Homo
Noc2l TGC TGCAGC 4: 156,324,553 (GRCm39) probably benign Het
Nrg3 G GACATTT 14: 38,119,230 (GRCm39) probably benign Homo
Or51q1 TCC TCCC 7: 103,629,110 (GRCm39) probably null Het
Padi3 TCTCAC TC 4: 140,520,283 (GRCm39) probably benign Homo
Patl2 GCT GCTTCT 2: 121,956,616 (GRCm39) probably benign Het
Pdik1l TTTT TTTTGTTTTTGGTTT 4: 134,006,685 (GRCm39) probably null Homo
Pik3c2g AG AGAGGG 6: 139,612,654 (GRCm39) probably null Homo
Plekhs1 T TTCAGACCTCCCC 19: 56,468,290 (GRCm39) probably benign Het
Prkd3 G T 17: 79,283,249 (GRCm39) probably null Homo
Prkn G A 17: 12,073,650 (GRCm39) V323M probably damaging Het
Prr13 TCC TCCCCC 15: 102,370,612 (GRCm39) probably benign Homo
Prrc2b G A 2: 32,111,179 (GRCm39) A1852T probably damaging Homo
Ptms CTT CTTTTT 6: 124,891,421 (GRCm39) probably benign Homo
Rtl1 TTCCTCTTCCTCCTC TTCCTC 12: 109,557,632 (GRCm39) probably benign Homo
Scaf4 TGCGGC TGC 16: 90,026,742 (GRCm39) probably benign Homo
Serac1 T A 17: 6,121,083 (GRCm39) K70N probably damaging Homo
Six3 CGG CGGTGG 17: 85,928,796 (GRCm39) probably benign Het
Sry GTG GTGCTG Y: 2,662,837 (GRCm39) probably benign Homo
Stard8 GGAAGA GGAAGAAGA X: 98,110,111 (GRCm39) probably benign Het
Supt20 TTCAGCA TTCAGCATCAGCA 3: 54,635,068 (GRCm39) probably benign Het
Supt20 CAGCAG CAGCAGTAGCAG 3: 54,635,085 (GRCm39) probably null Het
Supt20 AGCAGC AGCAGCGGCAGC 3: 54,635,083 (GRCm39) probably benign Het
Sytl1 CTCT C 4: 132,984,304 (GRCm39) probably benign Homo
Tcof1 AGC AGCGGC 18: 60,968,814 (GRCm39) probably benign Het
Tdpoz2 T TCA 3: 93,558,922 (GRCm39) probably null Homo
Tert GCC GCCAAGGGTTCC 13: 73,796,421 (GRCm39) probably benign Homo
Tfeb GCA GCAACA 17: 48,097,019 (GRCm39) probably benign Het
Ticrr ATT ATTTTT 7: 79,344,059 (GRCm39) probably benign Homo
Tnfaip8 ACACACACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC AC 18: 50,179,906 (GRCm39) probably null Homo
Tnfrsf9 T TGCC 4: 151,018,852 (GRCm39) probably benign Homo
Tob1 GCA GCAACA 11: 94,105,290 (GRCm39) probably benign Het
Tob1 CA CAGTA 11: 94,105,303 (GRCm39) probably null Het
Trav15-2-dv6-2 GGGAG GGGAGGAG 14: 53,887,207 (GRCm39) probably benign Homo
Triobp TCGTCG TCGTCGTCG 15: 78,877,587 (GRCm39) probably benign Homo
Tsbp1 A AGCC 17: 34,679,029 (GRCm39) probably benign Het
Tsbp1 GC GCATC 17: 34,679,051 (GRCm39) probably benign Het
Tsen2 AGG AGGGGG 6: 115,537,030 (GRCm39) probably benign Het
Ubtf TCC TCCGCC 11: 102,197,782 (GRCm39) probably benign Het
Ubtf CTCGTCGTC CTCGTCGTCGTC 11: 102,197,784 (GRCm39) probably benign Het
Vars1 TGG TGGAGTCCTGGGCGG 17: 35,234,965 (GRCm39) probably benign Homo
Vmn1r171 C T 7: 23,332,105 (GRCm39) A110V probably benign Het
Vmn2r31 G T 7: 7,387,607 (GRCm39) Q655K probably damaging Het
Vmn2r87 C T 10: 130,314,583 (GRCm39) M334I probably benign Homo
Zc3h13 CG CGAGATGTGTG 14: 75,561,050 (GRCm39) probably benign Het
Zc3h13 AGATGTGCG AGATGTGCGGGATGTGCG 14: 75,561,043 (GRCm39) probably benign Het
Zfp282 GGC GGCCGC 6: 47,881,731 (GRCm39) probably benign Het
Zfp384 AGGC AGGCCCAGGCCCCGGC 6: 125,013,456 (GRCm39) probably benign Het
Zfp459 TGA TGAGCGA 13: 67,556,393 (GRCm39) probably null Homo
Zfp462 GCCACC GCCACCTCAGCCACAACCACC 4: 55,009,757 (GRCm39) probably benign Het
Zfp462 CCACC CCACCTCAGCCACAGTCACC 4: 55,009,758 (GRCm39) probably benign Het
Zfp598 CACCAC CACCACAACCAC 17: 24,899,749 (GRCm39) probably benign Het
Zfp831 CCT CCTGCT 2: 174,487,274 (GRCm39) probably benign Het
Other mutations in Cfap74
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01607:Cfap74 APN 4 155,503,443 (GRCm39) missense possibly damaging 0.95
paranoid UTSW 4 155,538,481 (GRCm39) utr 5 prime probably benign
sensibile UTSW 4 155,525,743 (GRCm39) missense probably null 0.03
touchy UTSW 4 155,547,529 (GRCm39) missense probably benign 0.27
P0007:Cfap74 UTSW 4 155,506,685 (GRCm39) missense possibly damaging 0.83
PIT4434001:Cfap74 UTSW 4 155,548,421 (GRCm39) missense unknown
R0025:Cfap74 UTSW 4 155,510,572 (GRCm39) missense probably benign 0.22
R0034:Cfap74 UTSW 4 155,545,344 (GRCm39) splice site probably benign
R0193:Cfap74 UTSW 4 155,510,572 (GRCm39) missense probably benign 0.22
R0306:Cfap74 UTSW 4 155,549,896 (GRCm39) unclassified probably benign
R0427:Cfap74 UTSW 4 155,525,734 (GRCm39) missense probably benign 0.02
R0905:Cfap74 UTSW 4 155,503,153 (GRCm39) critical splice donor site probably null
R1116:Cfap74 UTSW 4 155,518,453 (GRCm39) missense probably benign 0.15
R1548:Cfap74 UTSW 4 155,518,502 (GRCm39) missense probably benign 0.45
R1950:Cfap74 UTSW 4 155,511,887 (GRCm39) critical splice donor site probably null
R2009:Cfap74 UTSW 4 155,504,724 (GRCm39) missense possibly damaging 0.77
R2029:Cfap74 UTSW 4 155,526,538 (GRCm39) missense possibly damaging 0.74
R2135:Cfap74 UTSW 4 155,514,408 (GRCm39) missense probably damaging 1.00
R2135:Cfap74 UTSW 4 155,514,397 (GRCm39) missense probably damaging 0.97
R2154:Cfap74 UTSW 4 155,513,753 (GRCm39) missense possibly damaging 0.94
R2413:Cfap74 UTSW 4 155,503,081 (GRCm39) missense possibly damaging 0.76
R2418:Cfap74 UTSW 4 155,540,166 (GRCm39) utr 5 prime probably benign
R2930:Cfap74 UTSW 4 155,522,627 (GRCm39) missense probably damaging 0.97
R3965:Cfap74 UTSW 4 155,531,174 (GRCm39) missense probably damaging 1.00
R4078:Cfap74 UTSW 4 155,540,128 (GRCm39) missense probably damaging 0.98
R4238:Cfap74 UTSW 4 155,547,529 (GRCm39) missense probably benign 0.27
R4239:Cfap74 UTSW 4 155,547,529 (GRCm39) missense probably benign 0.27
R4240:Cfap74 UTSW 4 155,547,529 (GRCm39) missense probably benign 0.27
R4491:Cfap74 UTSW 4 155,513,628 (GRCm39) missense probably benign 0.22
R4731:Cfap74 UTSW 4 155,548,059 (GRCm39) intron probably benign
R5333:Cfap74 UTSW 4 155,521,197 (GRCm39) missense probably damaging 0.99
R5362:Cfap74 UTSW 4 155,522,623 (GRCm39) missense probably damaging 0.98
R5425:Cfap74 UTSW 4 155,540,149 (GRCm39) utr 5 prime probably benign
R5468:Cfap74 UTSW 4 155,510,498 (GRCm39) missense probably benign 0.27
R5839:Cfap74 UTSW 4 155,507,207 (GRCm39) critical splice donor site probably null
R6010:Cfap74 UTSW 4 155,538,495 (GRCm39) missense possibly damaging 0.83
R6284:Cfap74 UTSW 4 155,536,253 (GRCm39) missense probably damaging 0.96
R6323:Cfap74 UTSW 4 155,548,395 (GRCm39) missense possibly damaging 0.54
R6389:Cfap74 UTSW 4 155,507,793 (GRCm39) missense possibly damaging 0.69
R6513:Cfap74 UTSW 4 155,525,743 (GRCm39) missense probably null 0.03
R6527:Cfap74 UTSW 4 155,506,722 (GRCm39) splice site probably null
R6785:Cfap74 UTSW 4 155,538,481 (GRCm39) utr 5 prime probably benign
R6980:Cfap74 UTSW 4 155,550,809 (GRCm39) unclassified probably benign
R7039:Cfap74 UTSW 4 155,538,565 (GRCm39) critical splice donor site probably null
R7077:Cfap74 UTSW 4 155,540,134 (GRCm39) missense unknown
R7116:Cfap74 UTSW 4 155,539,518 (GRCm39) missense unknown
R7202:Cfap74 UTSW 4 155,510,654 (GRCm39) splice site probably null
R7227:Cfap74 UTSW 4 155,545,405 (GRCm39) nonsense probably null
R7228:Cfap74 UTSW 4 155,549,507 (GRCm39) missense unknown
R7261:Cfap74 UTSW 4 155,549,831 (GRCm39) missense unknown
R7315:Cfap74 UTSW 4 155,547,476 (GRCm39) missense unknown
R7337:Cfap74 UTSW 4 155,544,472 (GRCm39) missense unknown
R7354:Cfap74 UTSW 4 155,549,804 (GRCm39) missense unknown
R7533:Cfap74 UTSW 4 155,500,200 (GRCm39) missense
R7673:Cfap74 UTSW 4 155,547,513 (GRCm39) missense unknown
R7798:Cfap74 UTSW 4 155,507,079 (GRCm39) missense
R7829:Cfap74 UTSW 4 155,513,694 (GRCm39) missense
R7897:Cfap74 UTSW 4 155,514,351 (GRCm39) missense
R8099:Cfap74 UTSW 4 155,539,489 (GRCm39) missense unknown
R8126:Cfap74 UTSW 4 155,511,831 (GRCm39) missense
R8769:Cfap74 UTSW 4 155,503,105 (GRCm39) missense
R8873:Cfap74 UTSW 4 155,539,465 (GRCm39) missense unknown
R8893:Cfap74 UTSW 4 155,531,152 (GRCm39) missense unknown
R8900:Cfap74 UTSW 4 155,521,187 (GRCm39) missense
R8954:Cfap74 UTSW 4 155,521,187 (GRCm39) missense
R8957:Cfap74 UTSW 4 155,521,187 (GRCm39) missense
R8982:Cfap74 UTSW 4 155,521,187 (GRCm39) missense
R9008:Cfap74 UTSW 4 155,503,121 (GRCm39) missense
R9406:Cfap74 UTSW 4 155,510,626 (GRCm39) nonsense probably null
R9606:Cfap74 UTSW 4 155,509,133 (GRCm39) missense
R9641:Cfap74 UTSW 4 155,549,054 (GRCm39) missense unknown
R9647:Cfap74 UTSW 4 155,549,373 (GRCm39) missense unknown
R9655:Cfap74 UTSW 4 155,522,665 (GRCm39) missense
R9761:Cfap74 UTSW 4 155,550,844 (GRCm39) missense unknown
R9797:Cfap74 UTSW 4 155,514,402 (GRCm39) missense
T0970:Cfap74 UTSW 4 155,547,574 (GRCm39) splice site probably null
X0066:Cfap74 UTSW 4 155,548,421 (GRCm39) missense probably damaging 1.00
Z1176:Cfap74 UTSW 4 155,510,575 (GRCm39) missense
Z1177:Cfap74 UTSW 4 155,539,370 (GRCm39) start gained probably benign
Predicted Primers PCR Primer
(F):5'- ATGGCTCTGCACCCTTAAG -3'
(R):5'- AGTCTGTAGCCTGAAGGGATG -3'

Sequencing Primer
(F):5'- ATGGCTCTGCACCCTTAAGTTATTAG -3'
(R):5'- CCAGGTCACATTCAAAGGATTG -3'
Posted On 2018-04-05