Mutagenetix > Incidental Mutation

Incidental Mutation 'FR4589:Las1l'

511434

Institutional Source

Beutler Lab

Gene Symbol

Las1l

Ensembl Gene

ENSMUSG00000057421

Gene Name

LAS1-like (S. cerevisiae)

Synonyms

Accession Numbers

Essential gene?

Not available question?

Stock #

FR4589 ()

Quality Score

217.468

Status

Not validated

Chromosome

Chromosomal Location

95935335-95956962 bp(-) (GRCm38)

Type of Mutation

small insertion (2 aa in frame mutation)

DNA Base Change (assembly)

TCTTCC to TCTTCCGCTTCC at 95940619 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000109495 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079987] [ENSMUST00000113864]

AlphaFold

A2BE28

Predicted Effect

probably benign
Transcript: ENSMUST00000079987

SMART Domains

Protein: ENSMUSP00000078901
Gene: ENSMUSG00000057421

Domain	Start	End	E-Value	Type
Pfam:Las1	27	173	1e-45	PFAM
low complexity region	183	205	N/A	INTRINSIC
coiled coil region	522	609	N/A	INTRINSIC
low complexity region	724	738	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000113864

SMART Domains

Protein: ENSMUSP00000109495
Gene: ENSMUSG00000057421

Domain	Start	End	E-Value	Type
Pfam:Las1	28	172	6e-45	PFAM
low complexity region	183	205	N/A	INTRINSIC
coiled coil region	539	626	N/A	INTRINSIC
low complexity region	741	755	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126605

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000137659

Coding Region Coverage

1x: 99.8%
3x: 99.5%
10x: 98.4%
20x: 96.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 124 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930402H24Rik	TCC	TCCCCC	2: 130,770,745 (GRCm38)		probably benign	Het
4930402H24Rik	CC	CCTGC	2: 130,770,752 (GRCm38)		probably benign	Het
4930548H24Rik	GAGAAG	GAG	5: 31,487,373 (GRCm38)		probably benign	Het
7530416G11Rik	T	A	15: 85,494,307 (GRCm38)	E45V	unknown	Homo
A530064D06Rik	GTAGGAAGCTTAG	GTAG	17: 48,163,381 (GRCm38)		probably benign	Homo
Anxa2	C	CCCA	9: 69,480,210 (GRCm38)		probably benign	Het
Apol6	GTTT	GTTTTTTT	15: 77,051,438 (GRCm38)		probably null	Het
Arrb2	C	T	11: 70,438,671 (GRCm38)	T269M	probably damaging	Homo
AY761185	CACTGTGGG	C	8: 20,943,903 (GRCm38)		probably null	Het
BC051142	AGC	AGCCGC	17: 34,460,073 (GRCm38)		probably benign	Het
BC051142	GCA	GCACCA	17: 34,460,053 (GRCm38)		probably benign	Het
Bcas3	G	A	11: 85,509,497 (GRCm38)	V431I	probably benign	Homo
Blm	TACC	TACCGACC	7: 80,463,770 (GRCm38)		probably null	Het
Btnl10	AGA	AGAGGA	11: 58,923,929 (GRCm38)		probably benign	Homo
Btnl4	T	A	17: 34,472,636 (GRCm38)	K293M	probably benign	Het
Catsper2	TGTC	TGTCGTC	2: 121,397,779 (GRCm38)		probably benign	Het
Cd109	ATTTAT	ATTTATTTATTTCTTTAT	9: 78,712,529 (GRCm38)		probably benign	Het
Cd164	G	T	10: 41,521,926 (GRCm38)	A59S	probably benign	Homo
Cd22	C	T	7: 30,878,082 (GRCm38)	R2H	possibly damaging	Homo
Chd4	C	T	6: 125,122,133 (GRCm38)	P1597L	probably benign	Homo
Chd4	CCCCTGCCCCTGCCACTGCCCCTGCC	CCCCTGCCCCTGCCCCTGCCACTGCCCCTGCC	6: 125,122,139 (GRCm38)		probably benign	Homo
Chga	AGC	AGCTGC	12: 102,561,402 (GRCm38)		probably benign	Het
Cluh	AGCC	AGCCTGGGCC	11: 74,669,531 (GRCm38)		probably benign	Het
Cnpy3	ACCC	ACCCCCC	17: 46,736,739 (GRCm38)		probably benign	Het
Cntnap1	CCCAGC	CCCAGCTCCAGC	11: 101,189,566 (GRCm38)		probably benign	Het
Cntnap1	AGCCCC	AGCCCCCGCCCC	11: 101,189,575 (GRCm38)		probably benign	Het
Cntnap1	CAGCCC	CAGCCCGAGCCC	11: 101,189,580 (GRCm38)		probably benign	Het
Cntnap1	AGCCCC	AGCCCCCGCCCC	11: 101,189,581 (GRCm38)		probably benign	Het
Col2a1	C	A	15: 97,988,981 (GRCm38)		probably null	Het
Col6a5	A	T	9: 105,934,174 (GRCm38)	N715K	unknown	Homo
Cttnbp2	ATT	ATTTCTGTT	6: 18,367,458 (GRCm38)		probably benign	Het
D230025D16Rik	G	A	8: 105,241,098 (GRCm38)	G207E	probably benign	Homo
Dbr1	AGGAGG	AGGAGGCGGAGG	9: 99,583,683 (GRCm38)		probably benign	Het
Dbr1	AGGAGG	AGGAGGGGGAGG	9: 99,583,680 (GRCm38)		probably benign	Het
Dbr1	AGGAGG	AGGAGGGGGAGG	9: 99,583,677 (GRCm38)		probably benign	Het
Dbr1	GGAGGA	GGAGGAAGAGGA	9: 99,583,696 (GRCm38)		probably benign	Het
Dclre1a	AGGCTTTG	AG	19: 56,544,123 (GRCm38)		probably benign	Het
Dcpp1	A	C	17: 23,881,454 (GRCm38)	K53Q	probably benign	Het
Dhx8	CGAGAC	CGAGACAGAGAC	11: 101,738,188 (GRCm38)		probably benign	Homo
Dnah12	G	T	14: 26,849,385 (GRCm38)	G2817V	probably damaging	Homo
Dthd1	C	CTT	5: 62,843,026 (GRCm38)		probably null	Homo
Efhd2	GCCGCC	GCCGCCTCCGCC	4: 141,874,764 (GRCm38)		probably benign	Het
Eps8	AC	ACTCGC	6: 137,517,069 (GRCm38)		probably null	Het
Ermn	TTC	TTCATC	2: 58,048,069 (GRCm38)		probably benign	Het
Fam81b	TC	TCTCC	13: 76,271,323 (GRCm38)		probably benign	Het
Fbrsl1	TG	TGCGTGTGCTGGCG	5: 110,378,150 (GRCm38)		probably benign	Het
Fbxo43	CCTGTG	CCTGTGTCTGTG	15: 36,152,101 (GRCm38)		probably benign	Het
Fbxo43	GCCTGT	GCCTGTTCCTGT	15: 36,152,100 (GRCm38)		probably benign	Het
Fmn1	TCCTCC	TCCTCCCCCTCC	2: 113,525,774 (GRCm38)		probably benign	Het
Fmn1	CTCCTC	CTCCTCTTCCTC	2: 113,525,773 (GRCm38)		probably benign	Het
Frmpd2	G	T	14: 33,511,021 (GRCm38)	L399F	probably damaging	Homo
Gabre	GCTCCGACTCCGACTCCG	GCTCCGACTCCGACTCCGACTCCG	X: 72,270,030 (GRCm38)		probably benign	Homo
Gabre	ACTCCGGCTCCGGCTCAGGCTCAGGCTCCGGCTCAGGCTCCGGCTCCGGCTCCGGCTC	ACTCCGGCTCCGGCTCCGGCTCAGGCTCAGGCTCCGGCTCAGGCTCCGGCTCCGGCTCCGGCTC	X: 72,270,042 (GRCm38)		probably benign	Homo
Gbp2b	A	G	3: 142,603,652 (GRCm38)	I175V	probably benign	Het
Gm10324	G	A	13: 66,122,208 (GRCm38)	S396N	probably benign	Het
Gm4340	AGC	AGCCGC	10: 104,196,079 (GRCm38)		probably benign	Het
Gm4340	CAG	CAGTAG	10: 104,196,078 (GRCm38)		probably null	Het
Gm4340	AG	AGCCG	10: 104,196,100 (GRCm38)		probably benign	Het
H2-Q4	G	A	17: 35,380,405 (GRCm38)	D155N	probably damaging	Het
Ighv5-9	C	T	12: 113,661,877 (GRCm38)	S82N	probably benign	Homo
Ipo9	TCC	TCCCCC	1: 135,386,281 (GRCm38)		probably benign	Het
Ipo9	CCATC	CCATCATC	1: 135,386,266 (GRCm38)		probably benign	Het
Isg20l2	GAAA	GAAAAAA	3: 87,931,717 (GRCm38)		probably benign	Homo
Klra9	C	G	6: 130,182,403 (GRCm38)	D216H	probably benign	Het
Kmt2b	TCC	TCCTCCGCC	7: 30,586,381 (GRCm38)		probably benign	Het
Kmt2b	CCTCCT	CCTCCTACTCCT	7: 30,586,364 (GRCm38)		probably null	Het
Kmt2b	CCTCCT	CCTCCTGCTCCT	7: 30,586,361 (GRCm38)		probably benign	Het
Krt10	TCC	TCCGCCGCC	11: 99,389,276 (GRCm38)		probably benign	Het
Lce1m	TGCTGCCACC	TGCTGCCACCACGGCTGCCACC	3: 93,018,268 (GRCm38)		probably benign	Homo
Lor	GCCGCCGCC	GC	3: 92,081,894 (GRCm38)		probably null	Het
Lrit3	AC	ACATCC	3: 129,803,913 (GRCm38)		probably null	Het
Lrrc63	CGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG	CGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG	14: 75,125,182 (GRCm38)		probably benign	Homo
Mapk7	GG	GGTGCTAG	11: 61,490,222 (GRCm38)		probably benign	Het
Med12l	GCAACA	GCAACAACA	3: 59,275,956 (GRCm38)		probably benign	Het
Nacad	GGGTCA	GGGTCATGGTCA	11: 6,599,753 (GRCm38)		probably benign	Het
Ndufc2	G	C	7: 97,400,290 (GRCm38)	M34I	probably benign	Het
Nphp3	CACG	C	9: 104,025,939 (GRCm38)		probably benign	Het
Nrg3	AG	AGCCTTTG	14: 38,397,266 (GRCm38)		probably benign	Het
Pdik1l	TTTTGTTTT	TTTTGTTTTGTGTTTGTTTT	4: 134,279,369 (GRCm38)		probably null	Homo
Pdik1l	TTTTTGTTTT	TTTTTGTTTTGATTTTGTTTT	4: 134,279,368 (GRCm38)		probably null	Homo
Plekhs1	AC	ACCTCCCCCGAGGC	19: 56,479,863 (GRCm38)		probably benign	Het
Prag1	CCGC	CCGCCGC	8: 36,103,883 (GRCm38)		probably benign	Homo
Prtg	G	A	9: 72,856,865 (GRCm38)	R540Q	probably damaging	Het
Raet1d	T	TCCTCTCTGGTAG	10: 22,370,918 (GRCm38)		probably null	Homo
Rhbdf1	A	ATTTT	11: 32,214,391 (GRCm38)		probably benign	Het
Rps19	AAAATT	AAAATTGAAATT	7: 24,889,182 (GRCm38)		probably benign	Het
Rtbdn	GGCAGC	GGCAGCCGCAGC	8: 84,956,171 (GRCm38)		probably benign	Het
Scaf4	TGCGGC	TGC	16: 90,229,854 (GRCm38)		probably benign	Homo
Serac1	T	A	17: 6,070,808 (GRCm38)	K70N	probably damaging	Homo
Setd1a	CTGGTGGTGGTGGTGGTGGTAGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG	CTGGTGGTGGTGGTGGTGGTGGTGGTGGTAGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG	7: 127,785,297 (GRCm38)		probably benign	Het
Shf	TCT	TCTGCT	2: 122,354,177 (GRCm38)		probably benign	Homo
Shroom4	TGCAGCAGCAGCAGCAGCA	TGCAGCAGCAGCAGCA	X: 6,624,061 (GRCm38)		probably benign	Homo
Six3	CGG	CGGAGG	17: 85,621,365 (GRCm38)		probably benign	Het
Snx1	TCT	TCTCCT	9: 66,104,926 (GRCm38)		probably benign	Homo
Spag17	AGG	AGGGGG	3: 100,056,245 (GRCm38)		probably benign	Het
Spag17	GGA	GGATGA	3: 100,056,258 (GRCm38)		probably benign	Het
Speer4a	C	A	5: 26,036,748 (GRCm38)	E127*	probably null	Het
Sry	ACTG	ACTGCTG	Y: 2,662,818 (GRCm38)		probably benign	Het
Supt20	A	AGCAGCT	3: 54,727,671 (GRCm38)		probably benign	Het
Supt20	CAGCAG	CAGCAGGAGCAG	3: 54,727,655 (GRCm38)		probably benign	Het
Supt20	GCAGCA	GCAGCATCAGCA	3: 54,727,651 (GRCm38)		probably benign	Het
Tcof1	GGGTA	G	18: 60,828,650 (GRCm38)		probably benign	Homo
Tert	C	CAAGGGTGCG	13: 73,648,304 (GRCm38)		probably benign	Het
Tmbim7	C	T	5: 3,670,064 (GRCm38)	R100C	possibly damaging	Het
Tmed6	C	CTAGA	8: 107,061,598 (GRCm38)		probably null	Homo
Tob1	CACA	CACAACA	11: 94,214,451 (GRCm38)		probably benign	Het
Tob1	CA	CAGCAA	11: 94,214,477 (GRCm38)		probably null	Het
Trcg1	AGCTCCTGTGTCTGT	A	9: 57,242,202 (GRCm38)		probably null	Homo
Trim16	A	AAGC	11: 62,820,695 (GRCm38)		probably benign	Homo
Tubgcp4	GTGA	G	2: 121,175,463 (GRCm38)		probably benign	Het
Tusc1	ACCGCC	ACCGCCCCCGCC	4: 93,335,307 (GRCm38)		probably benign	Het
Ubtf	CTTC	CTTCTTC	11: 102,306,945 (GRCm38)		probably benign	Het
Ubtf	CTCTTC	CTCTTCTTC	11: 102,306,943 (GRCm38)		probably benign	Het
Vars	GTGG	GTGGAGTCCTGGTTGG	17: 35,015,988 (GRCm38)		probably benign	Homo
Vmn2r52	C	T	7: 10,159,020 (GRCm38)	E731K	probably damaging	Het
Vmn2r87	C	T	10: 130,478,714 (GRCm38)	M334I	probably benign	Homo
Zc3h13	TGTGCGAG	TGTGCGAGGAGTGCGAG	14: 75,323,597 (GRCm38)		probably benign	Het
Zc3h13	GTGCGAGAT	GTGCGAGATTTGCGAGAT	14: 75,323,598 (GRCm38)		probably benign	Het
Zc3h13	CGGGATGTGCG	CGGGATGTGCGGGATGTGCG	14: 75,323,592 (GRCm38)		probably benign	Homo
Zfhx3	CAGCA	CAGCAACAGAAGCA	8: 108,956,101 (GRCm38)		probably benign	Het
Zfp282	GGC	GGCAGC	6: 47,904,791 (GRCm38)		probably benign	Het
Zfp459	GA	GAGTTA	13: 67,408,275 (GRCm38)		probably null	Homo
Zfp598	ACCACC	ACCACCCCCACC	17: 24,680,779 (GRCm38)		probably benign	Het
Zfp831	TCC	TCCCCC	2: 174,645,468 (GRCm38)		probably benign	Het

Other mutations in Las1l

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02049:Las1l	APN	X	95,937,453 (GRCm38)	missense	probably benign	0.04
IGL02152:Las1l	APN	X	95,953,302 (GRCm38)	missense	probably damaging	1.00
IGL02864:Las1l	APN	X	95,947,840 (GRCm38)	missense	possibly damaging	0.78
FR4304:Las1l	UTSW	X	95,940,821 (GRCm38)	small insertion	probably benign
FR4304:Las1l	UTSW	X	95,940,820 (GRCm38)	small insertion	probably benign
FR4340:Las1l	UTSW	X	95,940,622 (GRCm38)	small insertion	probably benign
FR4449:Las1l	UTSW	X	95,940,832 (GRCm38)	small insertion	probably benign
FR4548:Las1l	UTSW	X	95,940,823 (GRCm38)	small insertion	probably benign
FR4548:Las1l	UTSW	X	95,940,625 (GRCm38)	small insertion	probably benign
FR4589:Las1l	UTSW	X	95,940,625 (GRCm38)	small insertion	probably benign
FR4589:Las1l	UTSW	X	95,940,621 (GRCm38)	small deletion	probably benign
FR4737:Las1l	UTSW	X	95,940,829 (GRCm38)	small insertion	probably benign
FR4737:Las1l	UTSW	X	95,940,827 (GRCm38)	small insertion	probably benign
FR4737:Las1l	UTSW	X	95,940,821 (GRCm38)	small insertion	probably benign
FR4976:Las1l	UTSW	X	95,940,833 (GRCm38)	small insertion	probably benign
FR4976:Las1l	UTSW	X	95,940,832 (GRCm38)	small insertion	probably benign
FR4976:Las1l	UTSW	X	95,940,827 (GRCm38)	small insertion	probably benign
R1400:Las1l	UTSW	X	95,946,900 (GRCm38)	missense	possibly damaging	0.88
RF003:Las1l	UTSW	X	95,940,816 (GRCm38)	small insertion	probably benign
RF008:Las1l	UTSW	X	95,940,816 (GRCm38)	small insertion	probably benign
RF014:Las1l	UTSW	X	95,940,657 (GRCm38)	small deletion	probably benign
RF025:Las1l	UTSW	X	95,940,620 (GRCm38)	nonsense	probably null
RF042:Las1l	UTSW	X	95,940,620 (GRCm38)	small insertion	probably benign

Predicted Primers

PCR Primer
(F):5'- TCCCTGTGCAAGAGAGAAGG -3'
(R):5'- ACTGGATACCTTACATGAGGACC -3'

Sequencing Primer
(F):5'- GAGAGAAGGCCCCCACTTC -3'
(R):5'- GGATACCTTACATGAGGACCTACAAC -3'

Posted On

2018-04-05