|Institutional Source||Beutler Lab|
|Gene Name||monoacylglycerol O-acyltransferase 2|
|Is this an essential gene?||Probably non essential (E-score: 0.089)|
|Stock #||R6256 (G1)|
|Chromosomal Location||99219084-99238619 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to T at 99219895 bp|
|Amino Acid Change||Histidine to Glutamine at position 305 (H305Q)|
|Ref Sequence||ENSEMBL: ENSMUSP00000064041 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000064231]|
|Predicted Effect||probably damaging
AA Change: H305Q
PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
AA Change: H305Q
|Predicted Effect||noncoding transcript
|Meta Mutation Damage Score||0.6467|
|Coding Region Coverage||
|Validation Efficiency||99% (74/75)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is an enzyme that catalyzes the synthesis of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. The encoded protein is important in the uptake of dietary fat by the small intestine. This protein forms a complex with diacylglycerol O-acyltransferase 2 in the endoplasmic reticulum, and this complex catalyzes the synthesis of triacylglycerol. [provided by RefSeq, Dec 2015]
PHENOTYPE: Mice homozygous for a null allele exhibit resistance to diet induced obesity, hyperinsulinemia, hyperlipidemia, and steatosis with decreased lipid absorption and increased oxygen consumption when fed a high fat diet. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Mogat2||
(F):5'- ATCCCAGGATTAGGAGCTGC -3'
(R):5'- CTTATACACAAACATGGGGTGAGG -3'
(F):5'- ATTAGGAGCTGCCCCCTC -3'
(R):5'- TACACAAACATGGGGTGAGGGAAAG -3'