Incidental Mutation 'R6355:Wnt3a'
ID 512347
Institutional Source Beutler Lab
Gene Symbol Wnt3a
Ensembl Gene ENSMUSG00000009900
Gene Name wingless-type MMTV integration site family, member 3A
Synonyms Wnt-3a
MMRRC Submission 044507-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R6355 (G1)
Quality Score 225.009
Status Validated
Chromosome 11
Chromosomal Location 59138859-59181578 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 59166058 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Asparagine at position 74 (I74N)
Ref Sequence ENSEMBL: ENSMUSP00000010044 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000010044]
AlphaFold P27467
Predicted Effect probably damaging
Transcript: ENSMUST00000010044
AA Change: I74N

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000010044
Gene: ENSMUSG00000009900
AA Change: I74N

DomainStartEndE-ValueType
signal peptide 1 18 N/A INTRINSIC
WNT1 44 352 9.57e-218 SMART
Meta Mutation Damage Score 0.8589 question?
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.5%
  • 10x: 97.9%
  • 20x: 93.8%
Validation Efficiency 98% (41/42)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous null mutants die at embryonic day 10.5-12.5 with failed development of caudal somites, notochord and structures rostral to hindlimbs. Homozygotes for a hypomorphic allele have vertebral defects and a short tail due to loss of caudal vertebrae. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 43 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Agtr1a T G 13: 30,565,482 (GRCm39) F182L probably benign Het
Ahnak A G 19: 8,986,126 (GRCm39) E2470G probably benign Het
Arhgap20 T C 9: 51,755,020 (GRCm39) W418R probably damaging Het
Arhgap29 A G 3: 121,804,907 (GRCm39) D832G possibly damaging Het
Btbd2 A G 10: 80,481,183 (GRCm39) F325L possibly damaging Het
Chrna10 A T 7: 101,762,292 (GRCm39) probably null Het
Cyp26a1 T C 19: 37,687,377 (GRCm39) I188T possibly damaging Het
Dnajc13 A G 9: 104,080,469 (GRCm39) C871R probably damaging Het
Efcab3 T C 11: 104,896,511 (GRCm39) S4287P probably benign Het
Gm5519 A T 19: 33,802,471 (GRCm39) *171C probably null Het
Gnat1 C A 9: 107,554,623 (GRCm39) V81F probably benign Het
Gorab G A 1: 163,214,138 (GRCm39) A264V probably damaging Het
Ift172 A T 5: 31,441,501 (GRCm39) V211E probably benign Het
Ivl CCTGCTGCTGCTGCT CCTGCTGCTGCT 3: 92,479,217 (GRCm39) probably benign Het
Kif26b A C 1: 178,743,743 (GRCm39) S1280R probably damaging Het
Lce3e C T 3: 92,875,042 (GRCm39) probably benign Het
Magi1 A G 6: 94,260,177 (GRCm39) Y43H probably benign Het
Mon2 T A 10: 122,858,825 (GRCm39) M829L possibly damaging Het
Nmd3 G A 3: 69,636,680 (GRCm39) V96I probably benign Het
Ogfod1 T C 8: 94,789,610 (GRCm39) M437T probably benign Het
Or4f54 A G 2: 111,123,230 (GRCm39) I206V probably benign Het
Or5m9b T A 2: 85,905,216 (GRCm39) I44K probably benign Het
Or6b2 T C 1: 92,407,702 (GRCm39) T214A probably benign Het
Pabpc2 A G 18: 39,907,445 (GRCm39) S237G probably damaging Het
Parp4 T A 14: 56,839,757 (GRCm39) D488E possibly damaging Het
Phldb2 C T 16: 45,645,701 (GRCm39) M293I probably damaging Het
Poc1b G A 10: 98,965,436 (GRCm39) E80K probably damaging Het
Prdm5 C A 6: 65,860,578 (GRCm39) Q421K probably damaging Het
Rad54b A G 4: 11,604,989 (GRCm39) E479G possibly damaging Het
Ralgapa1 A G 12: 55,745,639 (GRCm39) S1239P probably damaging Het
Robo3 A G 9: 37,330,235 (GRCm39) V1052A possibly damaging Het
Satb2 G T 1: 56,987,356 (GRCm39) D83E probably damaging Het
Sccpdh T C 1: 179,498,165 (GRCm39) S70P probably benign Het
Scn3a A G 2: 65,291,643 (GRCm39) F1701S probably damaging Het
Srsf9 A G 5: 115,465,368 (GRCm39) M1V probably null Het
Stk32a T A 18: 43,430,659 (GRCm39) probably null Het
Sult2a5 A C 7: 13,396,462 (GRCm39) Y183S probably benign Het
Tiam2 CGGG CGGGG 17: 3,464,897 (GRCm39) probably null Het
Tnip2 G A 5: 34,656,541 (GRCm39) Q255* probably null Het
Vmn1r26 A G 6: 57,985,536 (GRCm39) W218R probably benign Het
Vmn2r61 A G 7: 41,916,659 (GRCm39) Y424C probably benign Het
Vmn2r86 T A 10: 130,291,763 (GRCm39) M1L probably damaging Het
Zfyve1 A T 12: 83,641,415 (GRCm39) H80Q probably benign Het
Other mutations in Wnt3a
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01108:Wnt3a APN 11 59,147,135 (GRCm39) missense probably benign 0.03
R0334:Wnt3a UTSW 11 59,147,144 (GRCm39) missense probably damaging 0.97
R3912:Wnt3a UTSW 11 59,140,828 (GRCm39) missense possibly damaging 0.91
R4041:Wnt3a UTSW 11 59,140,470 (GRCm39) missense probably damaging 1.00
R4980:Wnt3a UTSW 11 59,140,626 (GRCm39) missense probably damaging 1.00
R5413:Wnt3a UTSW 11 59,166,182 (GRCm39) missense probably benign 0.04
R5528:Wnt3a UTSW 11 59,166,106 (GRCm39) missense probably damaging 0.99
R5626:Wnt3a UTSW 11 59,181,409 (GRCm39) missense probably benign 0.17
R6700:Wnt3a UTSW 11 59,140,587 (GRCm39) missense probably damaging 0.96
R8552:Wnt3a UTSW 11 59,166,043 (GRCm39) missense probably damaging 0.96
Predicted Primers PCR Primer
(F):5'- GCTCTTTGCCAGCAGCTAAC -3'
(R):5'- GCCGATCACATGCACATAGC -3'

Sequencing Primer
(F):5'- TTTCCAAACCAGCCCAGGGG -3'
(R):5'- CCAGAGTCTGTCCGTTTCAAGAG -3'
Posted On 2018-04-27