Mutagenetix > Incidental Mutation

Incidental Mutation 'R6368:Myo9a'

512797

Institutional Source

Beutler Lab

Gene Symbol

Myo9a

Ensembl Gene

ENSMUSG00000039585

Gene Name

myosin IXa

Synonyms

C130068I12Rik, 4732465J09Rik

MMRRC Submission

044518-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6368 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

59658179-59836149 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 59832231 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 2587 (S2587T)

Ref Sequence

ENSEMBL: ENSMUSP00000117432 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000128341] [ENSMUST00000135298] [ENSMUST00000136740]

AlphaFold

Q8C170

Predicted Effect

probably benign
Transcript: ENSMUST00000128341
AA Change: S2516T

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000119401
Gene: ENSMUSG00000039585
AA Change: S2516T

Domain	Start	End	E-Value	Type
RA	14	112	5.57e-30	SMART
low complexity region	129	137	N/A	INTRINSIC
MYSc	140	1018	N/A	SMART
IQ	1019	1041	1.79e1	SMART
IQ	1042	1064	4.11e0	SMART
IQ	1074	1096	1.9e-2	SMART
IQ	1115	1137	1.01e-6	SMART
IQ	1138	1160	8.71e-2	SMART
low complexity region	1161	1173	N/A	INTRINSIC
coiled coil region	1265	1285	N/A	INTRINSIC
low complexity region	1372	1384	N/A	INTRINSIC
coiled coil region	1492	1539	N/A	INTRINSIC
Blast:MYSc	1685	1938	6e-89	BLAST
low complexity region	1982	1993	N/A	INTRINSIC
C1	2002	2050	2.6e-9	SMART
RhoGAP	2075	2250	3.36e-73	SMART
coiled coil region	2320	2360	N/A	INTRINSIC
low complexity region	2419	2438	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000135298
AA Change: S2587T

PolyPhen 2 Score 0.009 (Sensitivity: 0.96; Specificity: 0.77)

SMART Domains

Protein: ENSMUSP00000117432
Gene: ENSMUSG00000039585
AA Change: S2587T

Domain	Start	End	E-Value	Type
RA	14	112	5.57e-30	SMART
low complexity region	129	137	N/A	INTRINSIC
MYSc	140	1018	N/A	SMART
IQ	1019	1041	1.79e1	SMART
IQ	1042	1064	4.11e0	SMART
IQ	1074	1096	1.9e-2	SMART
IQ	1115	1137	1.01e-6	SMART
IQ	1138	1160	8.71e-2	SMART
low complexity region	1161	1173	N/A	INTRINSIC
coiled coil region	1265	1285	N/A	INTRINSIC
low complexity region	1372	1384	N/A	INTRINSIC
coiled coil region	1492	1539	N/A	INTRINSIC
low complexity region	1744	1759	N/A	INTRINSIC
low complexity region	2053	2064	N/A	INTRINSIC
C1	2073	2121	2.6e-9	SMART
RhoGAP	2146	2321	3.36e-73	SMART
coiled coil region	2391	2431	N/A	INTRINSIC
low complexity region	2490	2509	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000136740
AA Change: S2605T

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000122852
Gene: ENSMUSG00000039585
AA Change: S2605T

Domain	Start	End	E-Value	Type
RA	14	112	5.57e-30	SMART
low complexity region	129	137	N/A	INTRINSIC
MYSc	140	1018	N/A	SMART
IQ	1019	1041	1.79e1	SMART
IQ	1042	1064	4.11e0	SMART
IQ	1074	1096	1.9e-2	SMART
IQ	1115	1137	1.01e-6	SMART
IQ	1138	1160	8.71e-2	SMART
low complexity region	1161	1173	N/A	INTRINSIC
coiled coil region	1265	1285	N/A	INTRINSIC
low complexity region	1372	1384	N/A	INTRINSIC
coiled coil region	1492	1539	N/A	INTRINSIC
low complexity region	1744	1759	N/A	INTRINSIC
low complexity region	2053	2064	N/A	INTRINSIC
C1	2073	2121	2.6e-9	SMART
RhoGAP	2146	2321	3.36e-73	SMART
coiled coil region	2409	2449	N/A	INTRINSIC
low complexity region	2508	2527	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000215963

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.4%
20x: 95.6%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the myosin superfamily. The protein represents an unconventional myosin; it should not be confused with the conventional non-muscle myosin-9 (MYH9). Unconventional myosins contain the basic domains of conventional myosins and are further distinguished from class members by their tail domains. They function as actin-based molecular motors. Mutations in this gene have been associated with Bardet-Biedl Syndrome. [provided by RefSeq, Dec 2011]
PHENOTYPE: Homozygous KO leads to obstructive hydrocephaly caused by blockage of the third ventricle and the rostral aqueduct caused by developmental failures of their ependymal cells. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930563M21Rik	C	A	9: 55,897,416 (GRCm39)	E272D	possibly damaging	Het
Abi2	A	G	1: 60,492,810 (GRCm39)	T158A	possibly damaging	Het
Acacb	T	C	5: 114,354,884 (GRCm39)	S1221P	probably damaging	Het
Agbl1	A	G	7: 76,069,578 (GRCm39)	D370G	probably benign	Het
Apeh	A	G	9: 107,964,442 (GRCm39)	I487T	probably damaging	Het
Arid1b	C	A	17: 5,382,808 (GRCm39)	N1297K	possibly damaging	Het
Ascc3	G	A	10: 50,576,081 (GRCm39)	G779S	probably damaging	Het
Atp7b	A	C	8: 22,510,771 (GRCm39)		probably null	Het
Bsn	C	T	9: 107,988,513 (GRCm39)		probably benign	Het
Caps2	C	T	10: 112,030,873 (GRCm39)	Q268*	probably null	Het
Cnfn	C	T	7: 25,067,386 (GRCm39)		probably null	Het
Cr2	A	G	1: 194,850,780 (GRCm39)	S229P	probably damaging	Het
Cubn	T	C	2: 13,435,806 (GRCm39)	Y1050C	probably damaging	Het
Cubn	T	C	2: 13,480,934 (GRCm39)	E307G	probably damaging	Het
Cyb5r3	A	C	15: 83,044,325 (GRCm39)	Y182D	possibly damaging	Het
Dclre1a	A	T	19: 56,535,223 (GRCm39)	H120Q	probably benign	Het
Ddx6	T	C	9: 44,547,073 (GRCm39)	I457T	probably damaging	Het
E2f1	A	T	2: 154,406,396 (GRCm39)	H93Q	possibly damaging	Het
Fam186a	T	C	15: 99,841,198 (GRCm39)	K1682R	possibly damaging	Het
Farsb	A	G	1: 78,443,602 (GRCm39)		probably null	Het
Flii	A	T	11: 60,611,962 (GRCm39)	L347Q	probably damaging	Het
Galntl6	T	G	8: 59,364,475 (GRCm39)	T12P	probably damaging	Het
Gm11595	G	A	11: 99,663,381 (GRCm39)	R100C	unknown	Het
Gm21680	T	C	5: 26,174,034 (GRCm39)	N190S	probably damaging	Het
Ifit1bl2	T	C	19: 34,596,525 (GRCm39)	S364G	probably benign	Het
Kdm2a	A	G	19: 4,400,345 (GRCm39)	I234T	probably damaging	Het
Kdm5b	T	C	1: 134,526,945 (GRCm39)	C356R	probably damaging	Het
Kel	A	T	6: 41,665,785 (GRCm39)	C174*	probably null	Het
Krt16	T	A	11: 100,137,502 (GRCm39)	D401V	probably damaging	Het
Ltb4r1	A	C	14: 56,005,200 (GRCm39)	I168L	probably benign	Het
Luzp1	T	A	4: 136,269,091 (GRCm39)	M438K	probably benign	Het
Mtf1	C	T	4: 124,718,145 (GRCm39)	T281M	probably damaging	Het
Or13a21	G	T	7: 139,999,580 (GRCm39)	Y35*	probably null	Het
Or1ad1	T	A	11: 50,875,613 (GRCm39)	F28L	probably benign	Het
Or4f58	A	T	2: 111,851,896 (GRCm39)	I101N	probably damaging	Het
Or7e169	T	C	9: 19,757,705 (GRCm39)	D70G	probably damaging	Het
Pcm1	T	A	8: 41,746,581 (GRCm39)	F1221Y	probably benign	Het
Pnldc1	A	T	17: 13,124,751 (GRCm39)	N90K	probably damaging	Het
Prickle2	A	T	6: 92,397,218 (GRCm39)	L169Q	probably damaging	Het
Ralgps2	A	T	1: 156,712,144 (GRCm39)	L147I	probably damaging	Het
Rfx3	A	T	19: 27,746,009 (GRCm39)	L674Q	possibly damaging	Het
Rpl3	C	A	15: 79,966,745 (GRCm39)	L14F	probably damaging	Het
Rrbp1	C	T	2: 143,831,475 (GRCm39)	G231R	probably damaging	Het
Sema3d	C	T	5: 12,620,980 (GRCm39)	L529F	probably damaging	Het
Slain1	A	T	14: 103,894,391 (GRCm39)	T193S	probably benign	Het
Slc2a6	G	T	2: 26,914,599 (GRCm39)	Q256K	possibly damaging	Het
Slk	A	G	19: 47,608,622 (GRCm39)	E525G	possibly damaging	Het
Spsb4	G	T	9: 96,826,698 (GRCm39)	Q252K	probably benign	Het
Taf1b	A	G	12: 24,608,256 (GRCm39)	T552A	possibly damaging	Het
Tmprss15	T	C	16: 78,802,945 (GRCm39)		probably null	Het
Tph2	T	C	10: 115,015,231 (GRCm39)	H177R	probably damaging	Het
Ttll1	A	G	15: 83,373,818 (GRCm39)	S332P	probably damaging	Het
Twf2	T	G	9: 106,090,032 (GRCm39)	N128K	probably benign	Het
Vegfc	T	A	8: 54,634,265 (GRCm39)	C315S	probably damaging	Het
Vmn2r111	T	A	17: 22,790,889 (GRCm39)	K136N	probably benign	Het
Wnk2	T	C	13: 49,214,814 (GRCm39)	E496G	probably damaging	Het
Zfp148	C	A	16: 33,317,568 (GRCm39)	Q705K	probably damaging	Het
Zw10	C	T	9: 48,984,535 (GRCm39)	A539V	probably damaging	Het

Other mutations in Myo9a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00427:Myo9a	APN	9	59,750,342 (GRCm39)	splice site	probably benign
IGL00510:Myo9a	APN	9	59,739,464 (GRCm39)	splice site	probably benign
IGL00710:Myo9a	APN	9	59,782,594 (GRCm39)	missense	probably damaging	1.00
IGL00963:Myo9a	APN	9	59,807,655 (GRCm39)	missense	probably damaging	0.98
IGL01087:Myo9a	APN	9	59,697,361 (GRCm39)	missense	possibly damaging	0.93
IGL01145:Myo9a	APN	9	59,762,658 (GRCm39)	missense	probably benign	0.18
IGL01403:Myo9a	APN	9	59,778,846 (GRCm39)	missense	probably damaging	0.98
IGL01528:Myo9a	APN	9	59,686,957 (GRCm39)	missense	probably damaging	1.00
IGL01608:Myo9a	APN	9	59,778,119 (GRCm39)	nonsense	probably null
IGL01701:Myo9a	APN	9	59,791,877 (GRCm39)	critical splice donor site	probably null
IGL01918:Myo9a	APN	9	59,686,985 (GRCm39)	missense	probably damaging	1.00
IGL02026:Myo9a	APN	9	59,813,245 (GRCm39)	missense	probably damaging	0.99
IGL02139:Myo9a	APN	9	59,687,275 (GRCm39)	missense	probably benign	0.07
IGL02176:Myo9a	APN	9	59,777,836 (GRCm39)	missense	probably benign	0.45
IGL02272:Myo9a	APN	9	59,791,883 (GRCm39)	splice site	probably benign
IGL02283:Myo9a	APN	9	59,778,956 (GRCm39)	missense	probably benign	0.00
IGL02499:Myo9a	APN	9	59,722,669 (GRCm39)	splice site	probably benign
IGL02652:Myo9a	APN	9	59,771,211 (GRCm39)	missense	probably damaging	1.00
IGL02666:Myo9a	APN	9	59,832,187 (GRCm39)	missense	probably benign	0.02
IGL02878:Myo9a	APN	9	59,815,583 (GRCm39)	critical splice donor site	probably null
IGL02982:Myo9a	APN	9	59,815,491 (GRCm39)	nonsense	probably null
IGL03072:Myo9a	APN	9	59,716,725 (GRCm39)	missense	possibly damaging	0.83
IGL03090:Myo9a	APN	9	59,801,418 (GRCm39)	splice site	probably benign
IGL03111:Myo9a	APN	9	59,734,526 (GRCm39)	missense	probably benign	0.19
IGL03389:Myo9a	APN	9	59,776,890 (GRCm39)	missense	probably damaging	1.00
essentials	UTSW	9	59,802,149 (GRCm39)	missense	probably benign	0.09
necessities	UTSW	9	59,722,617 (GRCm39)	missense	probably damaging	1.00
PIT4402001:Myo9a	UTSW	9	59,777,719 (GRCm39)	missense	possibly damaging	0.83
R0013:Myo9a	UTSW	9	59,767,489 (GRCm39)	splice site	probably benign
R0013:Myo9a	UTSW	9	59,767,489 (GRCm39)	splice site	probably benign
R0018:Myo9a	UTSW	9	59,779,007 (GRCm39)	missense	probably benign	0.00
R0018:Myo9a	UTSW	9	59,779,007 (GRCm39)	missense	probably benign	0.00
R0329:Myo9a	UTSW	9	59,830,960 (GRCm39)	missense	probably damaging	1.00
R0423:Myo9a	UTSW	9	59,802,619 (GRCm39)	missense	probably damaging	1.00
R0521:Myo9a	UTSW	9	59,801,635 (GRCm39)	missense	probably damaging	1.00
R0607:Myo9a	UTSW	9	59,829,076 (GRCm39)	missense	probably benign	0.02
R0652:Myo9a	UTSW	9	59,779,209 (GRCm39)	missense	probably benign
R0653:Myo9a	UTSW	9	59,832,274 (GRCm39)	missense	probably damaging	1.00
R0723:Myo9a	UTSW	9	59,778,383 (GRCm39)	missense	probably benign	0.01
R0784:Myo9a	UTSW	9	59,803,828 (GRCm39)	splice site	probably benign
R0842:Myo9a	UTSW	9	59,778,350 (GRCm39)	missense	probably benign	0.02
R1055:Myo9a	UTSW	9	59,762,653 (GRCm39)	missense	probably benign	0.01
R1056:Myo9a	UTSW	9	59,739,484 (GRCm39)	missense	possibly damaging	0.64
R1195:Myo9a	UTSW	9	59,802,483 (GRCm39)	missense	probably damaging	1.00
R1195:Myo9a	UTSW	9	59,802,483 (GRCm39)	missense	probably damaging	1.00
R1195:Myo9a	UTSW	9	59,802,483 (GRCm39)	missense	probably damaging	1.00
R1615:Myo9a	UTSW	9	59,695,739 (GRCm39)	missense	possibly damaging	0.68
R1698:Myo9a	UTSW	9	59,775,464 (GRCm39)	missense	probably benign	0.05
R1715:Myo9a	UTSW	9	59,739,583 (GRCm39)	missense	probably damaging	0.99
R1981:Myo9a	UTSW	9	59,801,429 (GRCm39)	missense	probably benign
R2228:Myo9a	UTSW	9	59,801,463 (GRCm39)	missense	probably benign	0.06
R2272:Myo9a	UTSW	9	59,722,584 (GRCm39)	missense	probably damaging	1.00
R2327:Myo9a	UTSW	9	59,687,048 (GRCm39)	missense	probably benign	0.11
R2990:Myo9a	UTSW	9	59,832,172 (GRCm39)	missense	possibly damaging	0.95
R3161:Myo9a	UTSW	9	59,739,598 (GRCm39)	splice site	probably benign
R3721:Myo9a	UTSW	9	59,775,463 (GRCm39)	missense	probably benign
R3928:Myo9a	UTSW	9	59,802,566 (GRCm39)	missense	probably damaging	1.00
R4197:Myo9a	UTSW	9	59,802,149 (GRCm39)	missense	probably benign	0.09
R4212:Myo9a	UTSW	9	59,813,349 (GRCm39)	nonsense	probably null
R4610:Myo9a	UTSW	9	59,779,165 (GRCm39)	missense	probably benign
R4616:Myo9a	UTSW	9	59,728,932 (GRCm39)	missense	probably damaging	1.00
R4621:Myo9a	UTSW	9	59,778,355 (GRCm39)	missense	probably benign	0.00
R4623:Myo9a	UTSW	9	59,778,355 (GRCm39)	missense	probably benign	0.00
R4632:Myo9a	UTSW	9	59,776,947 (GRCm39)	missense	probably benign	0.00
R4657:Myo9a	UTSW	9	59,782,699 (GRCm39)	critical splice donor site	probably null
R4892:Myo9a	UTSW	9	59,731,525 (GRCm39)	missense	probably damaging	0.98
R4897:Myo9a	UTSW	9	59,803,800 (GRCm39)	missense	probably benign	0.07
R4966:Myo9a	UTSW	9	59,779,017 (GRCm39)	missense	probably benign	0.00
R4993:Myo9a	UTSW	9	59,768,755 (GRCm39)	nonsense	probably null
R5160:Myo9a	UTSW	9	59,779,085 (GRCm39)	missense	probably benign	0.24
R5233:Myo9a	UTSW	9	59,817,900 (GRCm39)	missense	probably damaging	1.00
R5271:Myo9a	UTSW	9	59,814,665 (GRCm39)	missense	probably damaging	1.00
R5308:Myo9a	UTSW	9	59,771,244 (GRCm39)	missense	probably damaging	1.00
R5367:Myo9a	UTSW	9	59,807,732 (GRCm39)	missense	probably damaging	0.96
R5432:Myo9a	UTSW	9	59,772,953 (GRCm39)	missense	possibly damaging	0.94
R5459:Myo9a	UTSW	9	59,791,803 (GRCm39)	missense	probably damaging	0.98
R5511:Myo9a	UTSW	9	59,687,495 (GRCm39)	missense	probably damaging	1.00
R5568:Myo9a	UTSW	9	59,781,911 (GRCm39)	missense	probably benign
R5573:Myo9a	UTSW	9	59,778,284 (GRCm39)	missense	probably benign
R5589:Myo9a	UTSW	9	59,802,527 (GRCm39)	nonsense	probably null
R5607:Myo9a	UTSW	9	59,771,227 (GRCm39)	missense	probably damaging	1.00
R5633:Myo9a	UTSW	9	59,775,467 (GRCm39)	missense	possibly damaging	0.60
R5885:Myo9a	UTSW	9	59,778,503 (GRCm39)	missense	probably benign
R6024:Myo9a	UTSW	9	59,762,671 (GRCm39)	missense	possibly damaging	0.68
R6086:Myo9a	UTSW	9	59,697,340 (GRCm39)	nonsense	probably null
R6146:Myo9a	UTSW	9	59,778,512 (GRCm39)	missense	probably benign	0.01
R6194:Myo9a	UTSW	9	59,777,033 (GRCm39)	missense	probably benign	0.00
R6213:Myo9a	UTSW	9	59,734,541 (GRCm39)	missense	probably damaging	1.00
R6550:Myo9a	UTSW	9	59,775,482 (GRCm39)	missense	probably damaging	1.00
R6612:Myo9a	UTSW	9	59,734,479 (GRCm39)	missense	probably damaging	1.00
R6665:Myo9a	UTSW	9	59,779,155 (GRCm39)	missense	probably benign	0.09
R6951:Myo9a	UTSW	9	59,802,051 (GRCm39)	missense	probably damaging	1.00
R7026:Myo9a	UTSW	9	59,722,617 (GRCm39)	missense	probably damaging	1.00
R7107:Myo9a	UTSW	9	59,778,098 (GRCm39)	missense	probably benign	0.44
R7310:Myo9a	UTSW	9	59,778,436 (GRCm39)	missense	probably benign	0.08
R7473:Myo9a	UTSW	9	59,802,527 (GRCm39)	missense	probably benign	0.31
R7723:Myo9a	UTSW	9	59,687,141 (GRCm39)	missense	probably damaging	1.00
R7823:Myo9a	UTSW	9	59,719,233 (GRCm39)	missense	probably damaging	1.00
R7824:Myo9a	UTSW	9	59,767,392 (GRCm39)	missense	probably damaging	1.00
R7965:Myo9a	UTSW	9	59,695,721 (GRCm39)	missense	probably damaging	1.00
R8031:Myo9a	UTSW	9	59,687,374 (GRCm39)	missense	probably benign	0.33
R8055:Myo9a	UTSW	9	59,814,743 (GRCm39)	missense	probably damaging	1.00
R8071:Myo9a	UTSW	9	59,781,931 (GRCm39)	missense	probably benign
R8250:Myo9a	UTSW	9	59,767,392 (GRCm39)	missense	probably damaging	1.00
R8260:Myo9a	UTSW	9	59,817,961 (GRCm39)	missense	probably benign	0.08
R8355:Myo9a	UTSW	9	59,817,130 (GRCm39)	missense	probably damaging	1.00
R8432:Myo9a	UTSW	9	59,687,548 (GRCm39)	missense	probably damaging	1.00
R8470:Myo9a	UTSW	9	59,739,573 (GRCm39)	missense	probably damaging	1.00
R8528:Myo9a	UTSW	9	59,767,423 (GRCm39)	missense	probably damaging	1.00
R8681:Myo9a	UTSW	9	59,775,394 (GRCm39)	missense	probably benign	0.16
R8690:Myo9a	UTSW	9	59,782,657 (GRCm39)	missense	probably benign
R8793:Myo9a	UTSW	9	59,791,850 (GRCm39)	missense	probably benign	0.03
R8812:Myo9a	UTSW	9	59,687,030 (GRCm39)	missense	probably benign	0.14
R9016:Myo9a	UTSW	9	59,775,427 (GRCm39)	nonsense	probably null
R9026:Myo9a	UTSW	9	59,716,757 (GRCm39)	missense	probably damaging	0.96
R9036:Myo9a	UTSW	9	59,687,584 (GRCm39)	nonsense	probably null
R9130:Myo9a	UTSW	9	59,739,514 (GRCm39)	missense	probably damaging	0.98
R9131:Myo9a	UTSW	9	59,768,772 (GRCm39)	missense	probably damaging	1.00
R9213:Myo9a	UTSW	9	59,772,922 (GRCm39)	missense	probably benign	0.04
R9498:Myo9a	UTSW	9	59,734,466 (GRCm39)	missense	probably damaging	1.00
R9575:Myo9a	UTSW	9	59,813,190 (GRCm39)	missense	probably damaging	1.00
R9651:Myo9a	UTSW	9	59,778,764 (GRCm39)	missense	probably damaging	0.96
R9672:Myo9a	UTSW	9	59,687,332 (GRCm39)	missense	probably benign	0.16
RF018:Myo9a	UTSW	9	59,776,869 (GRCm39)	missense	probably benign	0.00
RF019:Myo9a	UTSW	9	59,829,055 (GRCm39)	missense	probably benign	0.00
Z1176:Myo9a	UTSW	9	59,802,542 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AAGTCTCCATTTTACCGAGCTGC -3'
(R):5'- AGCCCAGTGATCCAGCATAC -3'

Sequencing Primer
(F):5'- CTTCAGCTTGTGAGGCCCAG -3'
(R):5'- CAATCTGTCTGCTGAAACGAGGC -3'

Posted On

2018-04-27