Mutagenetix > Incidental Mutations

Incidental Mutation 'R6360:Olfr461'

513061

Institutional Source

Beutler Lab

Gene Symbol

Olfr461

Ensembl Gene

ENSMUSG00000068259

Gene Name

olfactory receptor 461

Synonyms

MOR120-3, GA_x6K02T2P3E9-6973252-6974193

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R6360 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

40542171-40555050 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 40544713 bp

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Lysine at position 89 (Q89K)

Ref Sequence

ENSEMBL: ENSMUSP00000150632 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000089490] [ENSMUST00000215009]

Predicted Effect

possibly damaging
Transcript: ENSMUST00000089490
AA Change: Q89K

PolyPhen 2 Score 0.830 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000086917
Gene: ENSMUSG00000068259
AA Change: Q89K

Domain	Start	End	E-Value	Type
Pfam:7tm_4	30	306	2e-41	PFAM
Pfam:7tm_1	40	289	1.3e-20	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000215009
AA Change: Q89K

PolyPhen 2 Score 0.830 (Sensitivity: 0.84; Specificity: 0.93)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000215125

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000216766

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.3%
20x: 95.2%

Validation Efficiency

98% (59/60)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arhgap10	G	A	8: 77,259,202	Q657*	probably null	Het
Car15	T	C	16: 17,838,066	T560A	probably benign	Het
Cass4	C	T	2: 172,432,611	H769Y	probably damaging	Het
Cdh6	A	G	15: 13,041,460	I506T	possibly damaging	Het
Clstn3	G	T	6: 124,438,429	R659S	possibly damaging	Het
Cntnap5b	C	T	1: 100,431,736	R695*	probably null	Het
Cpsf1	CCCCTGCATGAGGCAGGTCCC	CCCC	15: 76,597,455		probably null	Het
Dab2ip	A	G	2: 35,710,266	H355R	probably benign	Het
Dennd2a	C	T	6: 39,493,142	A539T	probably benign	Het
Dnajc18	T	C	18: 35,686,709	E173G	probably damaging	Het
Dock7	T	C	4: 98,969,662	I1472V	probably benign	Het
Esco1	A	T	18: 10,574,931	F714I	probably damaging	Het
Fam107a	T	G	14: 8,299,619	H73P	probably damaging	Het
Fam46b	T	C	4: 133,486,756	F313L	probably damaging	Het
Flg	A	G	3: 93,290,601		probably benign	Het
Fyn	C	A	10: 39,526,883	T217K	possibly damaging	Het
Gbp9	T	C	5: 105,083,730	D330G	probably benign	Het
Gin1	T	C	1: 97,792,539	S509P	possibly damaging	Het
Gm15922	A	T	7: 3,736,504	L455Q	probably damaging	Het
Gm17067	A	C	7: 42,708,482	S199A	probably benign	Het
Grk4	A	T	5: 34,674,537	K50M	probably damaging	Het
Inpp4b	A	G	8: 81,902,852	H272R	probably benign	Het
Ipo7	T	A	7: 110,027,129	L48Q	probably damaging	Het
Kbtbd2	A	G	6: 56,779,206	I515T	probably damaging	Het
Kcnu1	T	A	8: 25,861,180	S190R	possibly damaging	Het
Kpnb1	T	C	11: 97,173,270	N336S	probably benign	Het
Lbr	G	T	1: 181,832,155	D158E	probably benign	Het
Mphosph10	T	C	7: 64,389,955	Q89R	probably benign	Het
Nectin3	T	A	16: 46,411,109	T21S	probably benign	Het
Numb	C	A	12: 83,797,262	R383L	probably damaging	Het
Olfr1370	A	T	13: 21,072,583	N239K	probably damaging	Het
Park2	T	C	17: 12,004,052	F363S	probably damaging	Het
Pcdhb11	A	T	18: 37,422,159	I181F	probably benign	Het
Pcgf2	T	A	11: 97,692,409		probably null	Het
Pdzd8	T	C	19: 59,300,983	T662A	probably benign	Het
Pex13	A	C	11: 23,655,690	V180G	probably benign	Het
Pkp4	T	A	2: 59,214,747	V22D	probably benign	Het
Ppp1r32	T	C	19: 10,479,481	N167D	probably damaging	Het
Prpf4b	A	C	13: 34,901,433	D954A	probably damaging	Het
Rfx8	T	C	1: 39,680,965	I317V	probably benign	Het
Rnaseh2b	T	C	14: 62,361,419	S198P	probably damaging	Het
Rock1	A	T	18: 10,116,778	C453S	possibly damaging	Het
Scarf1	G	T	11: 75,515,669	G260W	probably damaging	Het
Scyl1	T	C	19: 5,760,571	E538G	probably damaging	Het
Sec14l5	A	T	16: 5,172,995	I267F	probably damaging	Het
Senp6	T	A	9: 80,113,806	V256D	probably benign	Het
Sf3b4	G	A	3: 96,176,728		probably benign	Het
Ssh1	T	C	5: 113,961,347		probably null	Het
Tas2r143	A	C	6: 42,400,835	M200L	probably benign	Het
Tbc1d22a	C	T	15: 86,214,629	P19S	probably damaging	Het
Tmc5	T	A	7: 118,633,966	M1K	probably null	Het
Tnc	T	C	4: 64,000,733	Y1151C	probably damaging	Het
Tshz3	A	G	7: 36,769,441	E285G	probably damaging	Het
Txndc11	C	T	16: 11,084,792	V664M	probably damaging	Het
Ube2g1	A	T	11: 72,663,082	N20Y	probably damaging	Het
Ufl1	T	A	4: 25,265,476	I369L	probably benign	Het
Vwf	A	T	6: 125,683,526	T2666S	probably benign	Het
Yif1a	A	G	19: 5,092,341	M259V	probably benign	Het
Zbtb46	T	C	2: 181,391,455	D471G	probably damaging	Het

Other mutations in Olfr461

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02232:Olfr461	APN	6	40544046	missense	probably damaging	0.96
IGL03245:Olfr461	APN	6	40544142	missense	probably damaging	0.98
R0325:Olfr461	UTSW	6	40544123	missense	possibly damaging	0.95
R0835:Olfr461	UTSW	6	40544338	missense	probably benign	0.01
R1594:Olfr461	UTSW	6	40544347	missense	probably benign	0.00
R2437:Olfr461	UTSW	6	40544922	missense	probably benign	0.37
R6970:Olfr461	UTSW	6	40544656	missense	probably benign	0.31
R7252:Olfr461	UTSW	6	40544769	missense	probably benign	0.06
R7326:Olfr461	UTSW	6	40544895	missense	probably damaging	0.97
R8766:Olfr461	UTSW	6	40544551	missense	probably benign
RF003:Olfr461	UTSW	6	40544362	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CCATGACACTGTTACCATCCAG -3'
(R):5'- TTTTCCTGGCACCAAAGAACTAC -3'

Sequencing Primer
(F):5'- CCAGGTACAAGTATAGCTGCTCATG -3'
(R):5'- AGAACTACACCACATCCTTTTTGCG -3'

Posted On

2018-04-27