Mutagenetix > Incidental Mutation

Incidental Mutation 'R6332:Fn1'

513149

Institutional Source

Beutler Lab

Gene Symbol

Fn1

Ensembl Gene

ENSMUSG00000026193

Gene Name

fibronectin 1

Synonyms

Fn-1, Fn

MMRRC Submission

044486-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6332 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

71624679-71692359 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 71667230 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Arginine at position 834 (Q834R)

Ref Sequence

ENSEMBL: ENSMUSP00000140907 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055226] [ENSMUST00000186129] [ENSMUST00000187938] [ENSMUST00000188674] [ENSMUST00000188894] [ENSMUST00000189821] [ENSMUST00000190780]

AlphaFold

P11276

Predicted Effect

probably benign
Transcript: ENSMUST00000055226
AA Change: Q834R

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000054499
Gene: ENSMUSG00000026193
AA Change: Q834R

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1264	1346	4.22e-9	SMART
FN3	1357	1437	9.6e-13	SMART
FN3	1448	1527	1.82e-13	SMART
FN3	1538	1617	6.69e-12	SMART
FN3	1632	1711	2.72e-12	SMART
FN3	1722	1801	8.9e-8	SMART
FN3	1812	1891	1.66e-7	SMART
FN3	1904	1983	4.92e-10	SMART
FN3	1993	2074	3.64e-13	SMART
low complexity region	2148	2165	N/A	INTRINSIC
FN3	2193	2272	2.9e0	SMART
FN1	2296	2340	3.72e-19	SMART
FN1	2341	2383	2.49e-20	SMART
FN1	2385	2425	2.69e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000186129
AA Change: Q834R

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000141123
Gene: ENSMUSG00000026193
AA Change: Q834R

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	1.66e-7	SMART
FN3	1723	1802	4.92e-10	SMART
FN3	1812	1893	3.64e-13	SMART
low complexity region	1967	1984	N/A	INTRINSIC
FN3	2012	2091	2.9e0	SMART
FN1	2115	2159	3.72e-19	SMART
FN1	2160	2202	2.49e-20	SMART
FN1	2204	2244	2.69e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000186613

Predicted Effect

probably benign
Transcript: ENSMUST00000187938
AA Change: Q834R

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000140975
Gene: ENSMUSG00000026193
AA Change: Q834R

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	8.9e-8	SMART
FN3	1721	1800	1.66e-7	SMART
FN3	1813	1892	4.92e-10	SMART
FN3	1902	1983	3.64e-13	SMART
low complexity region	2032	2049	N/A	INTRINSIC
FN3	2077	2156	2.9e0	SMART
FN1	2180	2224	3.72e-19	SMART
FN1	2225	2267	2.49e-20	SMART
FN1	2269	2309	2.69e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000188674
AA Change: Q834R

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000140907
Gene: ENSMUSG00000026193
AA Change: Q834R

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	8.9e-8	SMART
FN3	1721	1800	1.66e-7	SMART
FN3	1813	1892	4.92e-10	SMART
FN3	1902	1981	6.79e-13	SMART
FN3	1983	2061	1.01e1	SMART
FN1	2085	2129	3.72e-19	SMART
FN1	2130	2172	2.49e-20	SMART
FN1	2174	2214	2.69e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000188894
AA Change: Q834R

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000140471
Gene: ENSMUSG00000026193
AA Change: Q834R

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	8.9e-8	SMART
FN3	1721	1800	1.66e-7	SMART
FN3	1813	1892	4.92e-10	SMART
FN3	1902	1983	3.64e-13	SMART
low complexity region	2057	2074	N/A	INTRINSIC
FN3	2102	2181	2.9e0	SMART
FN1	2205	2249	3.72e-19	SMART
FN1	2250	2292	2.49e-20	SMART
FN1	2294	2334	2.69e-16	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000189160

Predicted Effect

probably benign
Transcript: ENSMUST00000189821
AA Change: Q834R

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000139702
Gene: ENSMUSG00000026193
AA Change: Q834R

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	1.66e-7	SMART
FN3	1723	1802	4.92e-10	SMART
FN3	1812	1891	6.79e-13	SMART
FN3	1893	1971	1.01e1	SMART
FN1	1995	2039	3.72e-19	SMART
FN1	2040	2082	2.49e-20	SMART
FN1	2084	2124	2.69e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000190780
AA Change: Q834R

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000140816
Gene: ENSMUSG00000026193
AA Change: Q834R

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	1.66e-7	SMART
FN3	1723	1802	4.92e-10	SMART
FN3	1812	1893	3.64e-13	SMART
low complexity region	1942	1959	N/A	INTRINSIC
FN3	1987	2066	2.9e0	SMART
FN1	2090	2134	3.72e-19	SMART
FN1	2135	2177	2.49e-20	SMART
FN1	2179	2219	2.69e-16	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.0%
20x: 94.2%

Validation Efficiency

99% (67/68)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]
PHENOTYPE: Mutants are defective in mesodermal function. Null mutants are embryonic lethal with major patterning and organizational defects. Conditional mutants live and show increased neuronal apoptosis and susceptibility to induced cerebral ischemia. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933421I07Rik	C	A	7: 42,095,667 (GRCm39)	G194C	possibly damaging	Het
Adamtsl1	A	G	4: 86,135,248 (GRCm39)	K258E	probably damaging	Het
Afdn	A	T	17: 14,030,707 (GRCm39)	D206V	possibly damaging	Het
Agt	G	T	8: 125,284,572 (GRCm39)	Q389K	possibly damaging	Het
Ankrd44	A	T	1: 54,801,432 (GRCm39)	D298E	probably damaging	Het
Anks1	A	G	17: 28,271,709 (GRCm39)	S897G	probably benign	Het
Apol9b	A	G	15: 77,619,746 (GRCm39)		probably null	Het
Baz1a	T	C	12: 54,965,339 (GRCm39)	E705G	probably benign	Het
BC048679	C	T	7: 81,144,966 (GRCm39)	V126M	probably benign	Het
Cep295	A	G	9: 15,246,210 (GRCm39)	F749L	possibly damaging	Het
Cntrl	T	A	2: 35,018,036 (GRCm39)	I482K	possibly damaging	Het
Col6a3	A	G	1: 90,749,955 (GRCm39)	F293S	probably damaging	Het
Dnah12	T	G	14: 26,439,129 (GRCm39)	M527R	probably damaging	Het
Dnhd1	G	A	7: 105,343,273 (GRCm39)	R1539H	probably benign	Het
Ece1	A	G	4: 137,685,319 (GRCm39)	Y603C	probably damaging	Het
Eea1	G	A	10: 95,877,335 (GRCm39)	A1350T	possibly damaging	Het
Exoc3l4	A	T	12: 111,394,402 (GRCm39)	K507N	possibly damaging	Het
Flt3l	A	T	7: 44,783,091 (GRCm39)		probably null	Het
Haus5	C	T	7: 30,358,401 (GRCm39)	W298*	probably null	Het
Hycc2	T	A	1: 58,569,034 (GRCm39)	Y515F	probably damaging	Het
Ifih1	T	C	2: 62,469,827 (GRCm39)	N157D	possibly damaging	Het
Itga2	A	C	13: 114,980,009 (GRCm39)	M1064R	probably benign	Het
Itgae	A	G	11: 73,002,228 (GRCm39)		probably null	Het
Krtap10-4	T	C	10: 77,662,883 (GRCm39)		probably benign	Het
Lamp3	A	G	16: 19,518,431 (GRCm39)	C269R	probably damaging	Het
Lrp5	T	C	19: 3,709,355 (GRCm39)	D125G	probably damaging	Het
Matn2	A	C	15: 34,423,901 (GRCm39)	E586D	probably benign	Het
Mef2b	T	A	8: 70,616,789 (GRCm39)		probably null	Het
Mrps22	A	G	9: 98,483,524 (GRCm39)		probably null	Het
Mtmr2	T	C	9: 13,711,325 (GRCm39)	F445L	probably damaging	Het
Nxph4	A	G	10: 127,362,237 (GRCm39)	V218A	probably damaging	Het
Or1e23	A	G	11: 73,408,001 (GRCm39)	V8A	probably benign	Het
Or4f57	C	T	2: 111,791,091 (GRCm39)	G109D	probably damaging	Het
Or51k1	A	G	7: 103,660,980 (GRCm39)	S310P	probably benign	Het
Pdpk1	A	T	17: 24,325,896 (GRCm39)	V100D	probably damaging	Het
Phldb2	A	C	16: 45,594,609 (GRCm39)	S899A	probably benign	Het
Pnpla3	G	A	15: 84,056,983 (GRCm39)		probably null	Het
Rasal2	A	G	1: 157,126,757 (GRCm39)	Y94H	probably damaging	Het
Rlf	A	T	4: 121,006,019 (GRCm39)	I987N	possibly damaging	Het
Rnd2	C	T	11: 101,359,825 (GRCm39)	L57F	probably damaging	Het
Rprd2	A	G	3: 95,687,753 (GRCm39)	Y300H	probably damaging	Het
Setbp1	G	A	18: 78,826,584 (GRCm39)	S1343L	probably benign	Het
Sfswap	A	G	5: 129,648,105 (GRCm39)	K938E	possibly damaging	Het
Shld2	A	G	14: 33,990,129 (GRCm39)	V259A	probably benign	Het
Slc3a1	A	C	17: 85,335,860 (GRCm39)	M1L	probably damaging	Het
Slco5a1	C	T	1: 12,991,409 (GRCm39)	V427I	probably benign	Het
Ssbp2	A	T	13: 91,839,027 (GRCm39)	M300L	probably benign	Het
Ssc5d	A	G	7: 4,940,521 (GRCm39)	D878G	probably damaging	Het
Stk19	A	G	17: 35,043,574 (GRCm39)	L212P	probably damaging	Het
Stk39	T	C	2: 68,240,387 (GRCm39)	M115V	possibly damaging	Het
Syt17	A	C	7: 118,033,466 (GRCm39)	S181A	probably benign	Het
Taar7b	A	T	10: 23,875,849 (GRCm39)	N5Y	probably benign	Het
Tmc4	A	G	7: 3,680,421 (GRCm39)		probably null	Het
Tmem248	T	A	5: 130,258,310 (GRCm39)	M1K	probably null	Het
Tmem82	T	A	4: 141,343,721 (GRCm39)	Q183L	probably damaging	Het
Tpd52l1	T	C	10: 31,214,203 (GRCm39)	E142G	probably damaging	Het
Ttll2	A	T	17: 7,619,167 (GRCm39)	H253Q	probably damaging	Het
Ttn	T	C	2: 76,687,808 (GRCm39)		probably benign	Het
Ubd	A	G	17: 37,506,392 (GRCm39)	K93E	probably benign	Het
Ugt2b35	T	A	5: 87,149,415 (GRCm39)	F222Y	probably damaging	Het
Vmn2r10	G	T	5: 109,151,328 (GRCm39)	N95K	probably damaging	Het
Vwa8	T	C	14: 79,434,904 (GRCm39)	V1775A	probably benign	Het
Zdbf2	A	G	1: 63,346,981 (GRCm39)	K1787E	possibly damaging	Het
Zfp26	A	T	9: 20,348,582 (GRCm39)	F661I	probably damaging	Het
Zfp735	A	T	11: 73,602,504 (GRCm39)	K483*	probably null	Het
Zfp946	A	G	17: 22,673,519 (GRCm39)	E91G	probably damaging	Het
Zic5	C	A	14: 122,697,161 (GRCm39)	D485Y	unknown	Het
Zmynd8	A	T	2: 165,680,772 (GRCm39)	D236E	probably damaging	Het

Other mutations in Fn1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00236:Fn1	APN	1	71,692,032 (GRCm39)	missense	probably benign	0.28
IGL00402:Fn1	APN	1	71,680,322 (GRCm39)	missense	probably damaging	1.00
IGL00946:Fn1	APN	1	71,684,699 (GRCm39)	splice site	probably benign
IGL01311:Fn1	APN	1	71,667,299 (GRCm39)	missense	probably damaging	1.00
IGL01338:Fn1	APN	1	71,665,369 (GRCm39)	missense	probably damaging	0.98
IGL01353:Fn1	APN	1	71,626,098 (GRCm39)	missense	probably damaging	1.00
IGL01674:Fn1	APN	1	71,645,900 (GRCm39)	missense	probably damaging	1.00
IGL01701:Fn1	APN	1	71,669,012 (GRCm39)	splice site	probably benign
IGL01734:Fn1	APN	1	71,658,644 (GRCm39)	missense	probably damaging	1.00
IGL01788:Fn1	APN	1	71,652,996 (GRCm39)	missense	probably damaging	1.00
IGL02186:Fn1	APN	1	71,677,693 (GRCm39)	missense	probably damaging	1.00
IGL02398:Fn1	APN	1	71,657,829 (GRCm39)	splice site	probably null
IGL02425:Fn1	APN	1	71,680,302 (GRCm39)	splice site	probably benign
IGL02516:Fn1	APN	1	71,676,482 (GRCm39)	missense	possibly damaging	0.78
IGL02593:Fn1	APN	1	71,641,591 (GRCm39)	missense	probably benign
IGL02651:Fn1	APN	1	71,636,835 (GRCm39)	missense	possibly damaging	0.65
IGL02681:Fn1	APN	1	71,658,641 (GRCm39)	missense	probably damaging	1.00
IGL02890:Fn1	APN	1	71,637,531 (GRCm39)	critical splice donor site	probably null
IGL02929:Fn1	APN	1	71,634,821 (GRCm39)	critical splice donor site	probably null
IGL03036:Fn1	APN	1	71,668,932 (GRCm39)	missense	probably damaging	1.00
IGL03088:Fn1	APN	1	71,653,197 (GRCm39)	splice site	probably null
IGL03142:Fn1	APN	1	71,676,455 (GRCm39)	missense	probably damaging	1.00
IGL03172:Fn1	APN	1	71,680,421 (GRCm39)	missense	probably damaging	0.99
IGL03184:Fn1	APN	1	71,648,656 (GRCm39)	missense	probably benign	0.02
IGL03212:Fn1	APN	1	71,680,484 (GRCm39)	nonsense	probably null
IGL03246:Fn1	APN	1	71,663,455 (GRCm39)	missense	possibly damaging	0.89
IGL03367:Fn1	APN	1	71,636,712 (GRCm39)	missense	probably benign	0.27
depth	UTSW	1	71,647,072 (GRCm39)	missense	probably damaging	1.00
flooded	UTSW	1	71,636,675 (GRCm39)	missense	probably benign	0.01
R0684_Fn1_062	UTSW	1	71,634,968 (GRCm39)	splice site	probably null
series	UTSW	1	71,634,945 (GRCm39)	missense	probably damaging	1.00
PIT4514001:Fn1	UTSW	1	71,667,615 (GRCm39)	missense	probably benign	0.01
R0008:Fn1	UTSW	1	71,634,879 (GRCm39)	missense	probably damaging	0.98
R0112:Fn1	UTSW	1	71,648,812 (GRCm39)	missense	probably damaging	1.00
R0138:Fn1	UTSW	1	71,663,269 (GRCm39)	missense	possibly damaging	0.82
R0383:Fn1	UTSW	1	71,636,844 (GRCm39)	missense	probably damaging	0.99
R0386:Fn1	UTSW	1	71,634,945 (GRCm39)	missense	probably damaging	1.00
R0648:Fn1	UTSW	1	71,636,744 (GRCm39)	missense	possibly damaging	0.79
R0684:Fn1	UTSW	1	71,634,968 (GRCm39)	splice site	probably null
R1054:Fn1	UTSW	1	71,625,373 (GRCm39)	makesense	probably null
R1183:Fn1	UTSW	1	71,625,404 (GRCm39)	missense	probably damaging	0.98
R1405:Fn1	UTSW	1	71,681,237 (GRCm39)	missense	probably damaging	1.00
R1405:Fn1	UTSW	1	71,681,237 (GRCm39)	missense	probably damaging	1.00
R1414:Fn1	UTSW	1	71,640,462 (GRCm39)	splice site	probably benign
R1677:Fn1	UTSW	1	71,636,814 (GRCm39)	missense	probably benign	0.00
R1773:Fn1	UTSW	1	71,676,542 (GRCm39)	missense	probably damaging	1.00
R1830:Fn1	UTSW	1	71,663,418 (GRCm39)	missense	probably damaging	1.00
R1987:Fn1	UTSW	1	71,690,784 (GRCm39)	missense	probably damaging	1.00
R1989:Fn1	UTSW	1	71,690,784 (GRCm39)	missense	probably damaging	1.00
R2068:Fn1	UTSW	1	71,639,598 (GRCm39)	missense	probably damaging	1.00
R2113:Fn1	UTSW	1	71,665,323 (GRCm39)	missense	probably damaging	1.00
R2145:Fn1	UTSW	1	71,645,163 (GRCm39)	missense	probably damaging	1.00
R2246:Fn1	UTSW	1	71,667,694 (GRCm39)	missense	probably benign	0.10
R2273:Fn1	UTSW	1	71,653,102 (GRCm39)	missense	probably null	1.00
R2274:Fn1	UTSW	1	71,653,102 (GRCm39)	missense	probably null	1.00
R2275:Fn1	UTSW	1	71,653,102 (GRCm39)	missense	probably null	1.00
R2303:Fn1	UTSW	1	71,653,195 (GRCm39)	critical splice acceptor site	probably null
R2379:Fn1	UTSW	1	71,688,443 (GRCm39)	nonsense	probably null
R2382:Fn1	UTSW	1	71,687,278 (GRCm39)	missense	probably damaging	1.00
R2567:Fn1	UTSW	1	71,636,895 (GRCm39)	nonsense	probably null
R2864:Fn1	UTSW	1	71,641,578 (GRCm39)	missense	probably damaging	0.99
R3154:Fn1	UTSW	1	71,632,242 (GRCm39)	missense	probably damaging	1.00
R3837:Fn1	UTSW	1	71,692,314 (GRCm39)	splice site	probably null
R3844:Fn1	UTSW	1	71,648,733 (GRCm39)	missense	possibly damaging	0.61
R3886:Fn1	UTSW	1	71,679,465 (GRCm39)	missense	probably damaging	1.00
R3887:Fn1	UTSW	1	71,679,465 (GRCm39)	missense	probably damaging	1.00
R3888:Fn1	UTSW	1	71,679,465 (GRCm39)	missense	probably damaging	1.00
R3889:Fn1	UTSW	1	71,679,465 (GRCm39)	missense	probably damaging	1.00
R3905:Fn1	UTSW	1	71,647,072 (GRCm39)	missense	probably damaging	1.00
R3906:Fn1	UTSW	1	71,647,072 (GRCm39)	missense	probably damaging	1.00
R3907:Fn1	UTSW	1	71,647,072 (GRCm39)	missense	probably damaging	1.00
R3909:Fn1	UTSW	1	71,647,072 (GRCm39)	missense	probably damaging	1.00
R4611:Fn1	UTSW	1	71,663,337 (GRCm39)	nonsense	probably null
R4724:Fn1	UTSW	1	71,687,307 (GRCm39)	critical splice acceptor site	probably null
R4732:Fn1	UTSW	1	71,641,671 (GRCm39)	splice site	probably null
R4733:Fn1	UTSW	1	71,641,671 (GRCm39)	splice site	probably null
R4756:Fn1	UTSW	1	71,629,967 (GRCm39)	missense	probably damaging	1.00
R4809:Fn1	UTSW	1	71,691,959 (GRCm39)	intron	probably benign
R4839:Fn1	UTSW	1	71,681,242 (GRCm39)	missense	probably damaging	1.00
R4915:Fn1	UTSW	1	71,634,968 (GRCm39)	splice site	probably null
R4917:Fn1	UTSW	1	71,634,968 (GRCm39)	splice site	probably null
R4918:Fn1	UTSW	1	71,634,968 (GRCm39)	splice site	probably null
R5002:Fn1	UTSW	1	71,668,887 (GRCm39)	missense	possibly damaging	0.48
R5015:Fn1	UTSW	1	71,665,336 (GRCm39)	missense	probably damaging	0.98
R5022:Fn1	UTSW	1	71,663,338 (GRCm39)	missense	probably damaging	1.00
R5109:Fn1	UTSW	1	71,688,394 (GRCm39)	missense	probably damaging	1.00
R5267:Fn1	UTSW	1	71,668,863 (GRCm39)	missense	probably damaging	1.00
R5323:Fn1	UTSW	1	71,636,591 (GRCm39)	missense	probably benign	0.09
R5333:Fn1	UTSW	1	71,663,339 (GRCm39)	missense	probably damaging	1.00
R5631:Fn1	UTSW	1	71,629,355 (GRCm39)	missense	probably damaging	1.00
R5644:Fn1	UTSW	1	71,666,409 (GRCm39)	missense	probably damaging	1.00
R5754:Fn1	UTSW	1	71,639,481 (GRCm39)	missense	probably damaging	1.00
R5807:Fn1	UTSW	1	71,687,218 (GRCm39)	missense	probably damaging	1.00
R6053:Fn1	UTSW	1	71,638,449 (GRCm39)	missense	probably damaging	1.00
R6133:Fn1	UTSW	1	71,636,886 (GRCm39)	missense	probably damaging	1.00
R6186:Fn1	UTSW	1	71,676,449 (GRCm39)	missense	probably damaging	1.00
R6270:Fn1	UTSW	1	71,676,434 (GRCm39)	missense	probably damaging	1.00
R6431:Fn1	UTSW	1	71,687,003 (GRCm39)	splice site	probably null
R6571:Fn1	UTSW	1	71,665,349 (GRCm39)	missense	probably damaging	1.00
R6596:Fn1	UTSW	1	71,648,641 (GRCm39)	missense	probably damaging	1.00
R6862:Fn1	UTSW	1	71,653,066 (GRCm39)	missense	probably benign	0.43
R6898:Fn1	UTSW	1	71,639,572 (GRCm39)	missense	probably damaging	1.00
R6984:Fn1	UTSW	1	71,665,238 (GRCm39)	missense	probably damaging	1.00
R7107:Fn1	UTSW	1	71,666,408 (GRCm39)	missense	probably damaging	1.00
R7121:Fn1	UTSW	1	71,639,697 (GRCm39)	intron	probably benign
R7127:Fn1	UTSW	1	71,636,703 (GRCm39)	missense	probably benign	0.16
R7194:Fn1	UTSW	1	71,641,482 (GRCm39)	missense	probably damaging	1.00
R7274:Fn1	UTSW	1	71,667,272 (GRCm39)	missense	probably benign
R7285:Fn1	UTSW	1	71,676,498 (GRCm39)	missense	probably damaging	1.00
R7426:Fn1	UTSW	1	71,688,384 (GRCm39)	missense	probably damaging	1.00
R7453:Fn1	UTSW	1	71,630,039 (GRCm39)	missense	probably damaging	1.00
R7508:Fn1	UTSW	1	71,636,675 (GRCm39)	missense	probably benign	0.01
R7724:Fn1	UTSW	1	71,642,894 (GRCm39)	missense	probably benign	0.02
R7848:Fn1	UTSW	1	71,689,760 (GRCm39)	missense	probably damaging	1.00
R7992:Fn1	UTSW	1	71,638,825 (GRCm39)	missense	probably benign	0.34
R8036:Fn1	UTSW	1	71,629,310 (GRCm39)	nonsense	probably null
R8077:Fn1	UTSW	1	71,651,761 (GRCm39)	missense	probably damaging	1.00
R8175:Fn1	UTSW	1	71,638,824 (GRCm39)	missense	probably damaging	1.00
R8177:Fn1	UTSW	1	71,648,746 (GRCm39)	missense	probably benign
R8212:Fn1	UTSW	1	71,682,064 (GRCm39)	missense	probably benign	0.01
R8322:Fn1	UTSW	1	71,667,618 (GRCm39)	missense	probably benign	0.04
R8745:Fn1	UTSW	1	71,676,528 (GRCm39)	missense	probably benign	0.00
R8780:Fn1	UTSW	1	71,682,308 (GRCm39)	missense	probably benign	0.00
R8805:Fn1	UTSW	1	71,644,239 (GRCm39)	missense	probably benign	0.27
R8927:Fn1	UTSW	1	71,638,535 (GRCm39)	missense	probably benign	0.16
R8928:Fn1	UTSW	1	71,638,535 (GRCm39)	missense	probably benign	0.16
R8928:Fn1	UTSW	1	71,641,777 (GRCm39)	intron	probably benign
R8989:Fn1	UTSW	1	71,663,446 (GRCm39)	missense	possibly damaging	0.95
R8991:Fn1	UTSW	1	71,676,491 (GRCm39)	missense	probably benign	0.05
R9095:Fn1	UTSW	1	71,647,149 (GRCm39)	missense	probably null	0.02
R9455:Fn1	UTSW	1	71,647,112 (GRCm39)	missense	probably benign
R9589:Fn1	UTSW	1	71,668,941 (GRCm39)	missense	probably damaging	1.00
R9631:Fn1	UTSW	1	71,625,387 (GRCm39)	missense	probably benign	0.01
R9645:Fn1	UTSW	1	71,667,629 (GRCm39)	missense	probably benign	0.35
R9723:Fn1	UTSW	1	71,663,369 (GRCm39)	missense	possibly damaging	0.95
X0023:Fn1	UTSW	1	71,637,532 (GRCm39)	critical splice donor site	probably null
Z1088:Fn1	UTSW	1	71,688,451 (GRCm39)	missense	probably damaging	1.00
Z1176:Fn1	UTSW	1	71,636,570 (GRCm39)	missense	probably benign	0.10

Predicted Primers

PCR Primer
(F):5'- CGAAGAATCCCTGGAAGATTTGAAG -3'
(R):5'- TGCTGAGGGTAGAGATCCAG -3'

Sequencing Primer
(F):5'- TCCCTGGAAGATTTGAAGTGGACAC -3'
(R):5'- GGCTAGCCTGCAGTAAGAACC -3'

Posted On

2018-04-27