|Institutional Source||Beutler Lab|
|Gene Name||protein kinase, membrane associated tyrosine/threonine 1|
|Is this an essential gene?||Probably essential (E-score: 0.954)|
|Stock #||R6358 (G1)|
|Chromosomal Location||23726336-23736729 bp(+) (GRCm38)|
|Type of Mutation||intron|
|DNA Base Change (assembly)||T to A at 23733656 bp|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000024701 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000024701] [ENSMUST00000024702]|
|Predicted Effect||probably null
|Predicted Effect||probably benign
|Coding Region Coverage||
|Validation Efficiency||100% (61/61)|
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Pkmyt1||
(F):5'- AGTAGCTTTCCTTTATGCAAGC -3'
(R):5'- TTAACAGCATAGAGTCGCCCATC -3'
(F):5'- GCTTTCCTTTATGCAAGCATTATTG -3'
(R):5'- TTCCTTAGAGCGCACCTGGAAG -3'