Mutagenetix > Incidental Mutation

Incidental Mutation 'R6351:Fat1'

514256

Institutional Source

Beutler Lab

Gene Symbol

Fat1

Ensembl Gene

ENSMUSG00000070047

Gene Name

FAT atypical cadherin 1

Synonyms

mFat1, Fath, 2310038E12Rik

Accession Numbers

Genbank: NM_001081286

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6351 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

44935447-45052257 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 45033495 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Leucine at position 3362 (Q3362L)

Ref Sequence

ENSEMBL: ENSMUSP00000149194 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098796] [ENSMUST00000189017] [ENSMUST00000191428] [ENSMUST00000215588]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000098796
AA Change: Q3339L

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000096394
Gene: ENSMUSG00000070047
AA Change: Q3339L

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
CA	62	148	3.05e-6	SMART
CA	172	256	3.29e-20	SMART
Blast:CA	277	382	5e-47	BLAST
CA	387	462	2.13e-5	SMART
CA	486	568	8.35e-22	SMART
CA	592	670	2.11e-2	SMART
CA	740	821	5.09e-26	SMART
CA	845	926	6.27e-26	SMART
CA	950	1031	4.07e-25	SMART
CA	1057	1138	5.13e-31	SMART
CA	1162	1244	8.79e-30	SMART
CA	1276	1351	2.06e-3	SMART
CA	1379	1456	1.63e-15	SMART
CA	1480	1562	3.29e-20	SMART
CA	1586	1667	2.34e-16	SMART
CA	1691	1765	1.16e-20	SMART
CA	1796	1879	6.27e-26	SMART
CA	1903	1979	1.47e-8	SMART
CA	2003	2081	2.65e-15	SMART
CA	2105	2181	2.14e-10	SMART
CA	2203	2283	9.82e-19	SMART
CA	2307	2390	7.54e-29	SMART
CA	2414	2492	3.29e-11	SMART
CA	2516	2596	6.48e-19	SMART
CA	2620	2703	3.48e-10	SMART
CA	2719	2809	2.26e-9	SMART
CA	2833	2918	8.08e-29	SMART
CA	2942	3023	5.99e-23	SMART
CA	3047	3125	2.63e-28	SMART
CA	3149	3230	2.79e-32	SMART
CA	3254	3335	5.25e-28	SMART
CA	3359	3440	4.46e-31	SMART
CA	3464	3545	1.25e-11	SMART
CA	3569	3641	5.67e-2	SMART
LamG	3853	3987	6.51e-36	SMART
EGF	4018	4052	8.57e-5	SMART
EGF	4057	4090	3.94e-4	SMART
EGF	4094	4127	4.29e-5	SMART
EGF_CA	4129	4165	1.81e-12	SMART
transmembrane domain	4182	4204	N/A	INTRINSIC
low complexity region	4308	4324	N/A	INTRINSIC
low complexity region	4436	4457	N/A	INTRINSIC
low complexity region	4472	4483	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000189017
AA Change: Q3339L

Predicted Effect

probably damaging
Transcript: ENSMUST00000191428
AA Change: Q3339L

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000140596
Gene: ENSMUSG00000070047
AA Change: Q3339L

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
CA	62	148	3.05e-6	SMART
CA	172	256	3.29e-20	SMART
Blast:CA	277	382	5e-47	BLAST
CA	387	462	2.13e-5	SMART
CA	486	568	8.35e-22	SMART
CA	592	670	2.11e-2	SMART
CA	740	821	5.09e-26	SMART
CA	845	926	6.27e-26	SMART
CA	950	1031	4.07e-25	SMART
CA	1057	1138	5.13e-31	SMART
CA	1162	1244	8.79e-30	SMART
CA	1276	1351	2.06e-3	SMART
CA	1379	1456	1.63e-15	SMART
CA	1480	1562	3.29e-20	SMART
CA	1586	1667	2.34e-16	SMART
CA	1691	1765	1.16e-20	SMART
CA	1796	1879	6.27e-26	SMART
CA	1903	1979	1.47e-8	SMART
CA	2003	2081	2.65e-15	SMART
CA	2105	2181	2.14e-10	SMART
CA	2203	2283	9.82e-19	SMART
CA	2307	2390	7.54e-29	SMART
CA	2414	2492	3.29e-11	SMART
CA	2516	2596	6.48e-19	SMART
CA	2620	2703	3.48e-10	SMART
CA	2719	2809	2.26e-9	SMART
CA	2833	2918	8.08e-29	SMART
CA	2942	3023	5.99e-23	SMART
CA	3047	3125	2.63e-28	SMART
CA	3149	3230	2.79e-32	SMART
CA	3254	3335	5.25e-28	SMART
CA	3359	3440	4.46e-31	SMART
CA	3464	3545	1.25e-11	SMART
CA	3569	3641	5.67e-2	SMART
LamG	3853	3987	6.51e-36	SMART
EGF	4018	4052	8.57e-5	SMART
EGF	4057	4090	3.94e-4	SMART
EGF	4094	4127	4.29e-5	SMART
EGF_CA	4129	4165	1.81e-12	SMART
transmembrane domain	4182	4204	N/A	INTRINSIC
low complexity region	4308	4324	N/A	INTRINSIC
low complexity region	4436	4457	N/A	INTRINSIC
low complexity region	4472	4483	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000215588
AA Change: Q3362L

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 94.9%

Validation Efficiency

99% (95/96)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is an ortholog of the Drosophila fat gene, which encodes a tumor suppressor essential for controlling cell proliferation during Drosophila development. The gene product is a member of the cadherin superfamily, a group of integral membrane proteins characterized by the presence of cadherin-type repeats. In addition to containing 34 tandem cadherin-type repeats, the gene product has five epidermal growth factor (EGF)-like repeats and one laminin A-G domain. This gene is expressed at high levels in a number of fetal epithelia. Its product probably functions as an adhesion molecule and/or signaling receptor, and is likely to be important in developmental processes and cell communication. Transcript variants derived from alternative splicing and/or alternative promoter usage exist, but they have not been fully described. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for a targeted null mutation exhibit holoprosencephaly, anophthalmia, kidney defects and perinatal lethality. Mice homozygous for a hypomorphic allele exhibit altered shoulder girdle and facial musculature, retinal defects, abnormal inner earpatterning and kidney defects. [provided by MGI curators]

Allele List at MGI

All alleles(56) : Targeted, other(1) Gene trapped(55)

Other mutations in this stock

Total: 95 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700025F22Rik	C	T	19: 11,142,401 (GRCm38)	G14D	probably damaging	Het
4932438A13Rik	G	A	3: 36,908,228 (GRCm38)	A493T	probably damaging	Het
Acaa1a	C	A	9: 119,341,564 (GRCm38)	S17*	probably null	Het
Adamts16	T	A	13: 70,836,203 (GRCm38)	S114C	probably damaging	Het
Agpat5	T	C	8: 18,846,708 (GRCm38)	V50A	probably benign	Het
Agrn	T	C	4: 156,179,434 (GRCm38)	N124S	probably benign	Het
AI481877	T	C	4: 59,069,317 (GRCm38)	D703G	probably benign	Het
Ak8	T	C	2: 28,735,626 (GRCm38)	I227T	probably benign	Het
Akap6	A	G	12: 53,142,025 (GRCm38)	E2074G	probably damaging	Het
Apc	T	A	18: 34,312,212 (GRCm38)	S702R	probably damaging	Het
Ascc3	T	C	10: 50,720,673 (GRCm38)	I1233T	probably damaging	Het
Asphd2	A	T	5: 112,385,832 (GRCm38)	F318I	probably damaging	Het
Bnc2	T	C	4: 84,293,143 (GRCm38)	T397A	probably benign	Het
Bpifb4	C	T	2: 153,957,134 (GRCm38)	T528I	probably damaging	Het
Brinp3	A	G	1: 146,901,585 (GRCm38)	E590G	probably damaging	Het
Ccdc149	A	G	5: 52,385,135 (GRCm38)	S372P	probably benign	Het
Cdhr2	A	G	13: 54,726,776 (GRCm38)	H887R	probably benign	Het
Clcn6	C	T	4: 148,017,500 (GRCm38)	V376I	probably benign	Het
Cntln	T	G	4: 85,115,354 (GRCm38)	C1305W	probably damaging	Het
Cspg4	A	G	9: 56,892,644 (GRCm38)	D1564G	probably benign	Het
Cux1	A	T	5: 136,309,792 (GRCm38)	S582T	probably damaging	Het
Cwh43	G	A	5: 73,411,905 (GRCm38)	A97T	possibly damaging	Het
Dsc1	A	T	18: 20,086,769 (GRCm38)	F781L	probably damaging	Het
Dsc3	G	T	18: 19,966,291 (GRCm38)	H723N	probably benign	Het
Ear10	A	T	14: 43,923,055 (GRCm38)	V105D	probably damaging	Het
Ecel1	A	G	1: 87,149,509 (GRCm38)	V659A	possibly damaging	Het
Eogt	A	T	6: 97,120,194 (GRCm38)	F316I	probably damaging	Het
Etaa1	T	C	11: 17,947,188 (GRCm38)	N310D	possibly damaging	Het
Exoc3l2	G	A	7: 19,469,708 (GRCm38)	R75Q	possibly damaging	Het
Fam186a	A	C	15: 99,941,742 (GRCm38)	L2207R	probably damaging	Het
Fam187b	T	C	7: 30,977,599 (GRCm38)	Y178H	probably damaging	Het
Fat3	A	T	9: 15,938,398 (GRCm38)	S3903T	probably damaging	Het
Fsip2	G	A	2: 82,992,684 (GRCm38)	V6254I	possibly damaging	Het
Gabra5	T	C	7: 57,413,780 (GRCm38)	T299A	probably damaging	Het
Gbp11	T	C	5: 105,327,598 (GRCm38)	T295A	probably benign	Het
Glcci1	C	T	6: 8,573,203 (GRCm38)	Q44*	probably null	Het
Gpc5	A	T	14: 115,399,200 (GRCm38)	T432S	probably benign	Het
Grhl1	G	A	12: 24,584,858 (GRCm38)	E228K	probably damaging	Het
Hyal5	T	C	6: 24,891,709 (GRCm38)		probably null	Het
Idh2	TCCCAGG	T	7: 80,098,331 (GRCm38)		probably benign	Het
Ints9	A	G	14: 64,993,007 (GRCm38)	I128V	probably damaging	Het
Kcnt2	A	T	1: 140,375,112 (GRCm38)	N130I	probably damaging	Het
Krba1	T	C	6: 48,414,128 (GRCm38)	V717A	probably benign	Het
Lrp12	T	A	15: 39,878,188 (GRCm38)	D377V	probably damaging	Het
Magi1	G	T	6: 93,943,229 (GRCm38)	D135E	possibly damaging	Het
Map10	T	A	8: 125,671,245 (GRCm38)	L459Q	probably damaging	Het
Mitf	A	G	6: 98,003,912 (GRCm38)	D238G	possibly damaging	Het
Mylk	G	T	16: 34,921,971 (GRCm38)	R951L	probably benign	Het
Nacad	T	A	11: 6,600,165 (GRCm38)	K1009*	probably null	Het
Nacad	T	A	11: 6,599,235 (GRCm38)	D1272V	probably damaging	Het
Ncor1	T	C	11: 62,373,298 (GRCm38)	D786G	probably benign	Het
Oas2	G	A	5: 120,748,538 (GRCm38)	R188C	probably benign	Het
Odf2l	A	G	3: 145,135,718 (GRCm38)	I300V	probably benign	Het
Olfr1502	A	G	19: 13,861,822 (GRCm38)	T10A	probably benign	Het
Olfr338	T	A	2: 36,377,196 (GRCm38)	V140D	possibly damaging	Het
Olfr484	A	G	7: 108,124,430 (GRCm38)	S278P	probably damaging	Het
Olfr624	A	G	7: 103,670,956 (GRCm38)	I25T	possibly damaging	Het
Olfr64	A	T	7: 103,893,135 (GRCm38)	L200*	probably null	Het
Pes1	T	A	11: 3,978,865 (GRCm38)	D574E	probably benign	Het
Phf20	C	T	2: 156,294,210 (GRCm38)	R650C	possibly damaging	Het
Pias4	G	A	10: 81,157,264 (GRCm38)	T248I	probably damaging	Het
Pkhd1	T	C	1: 20,211,951 (GRCm38)	T2889A	probably benign	Het
Plcxd1	C	A	5: 110,102,167 (GRCm38)		probably null	Het
Plekha7	A	G	7: 116,176,898 (GRCm38)	F194L	probably damaging	Het
Plxnb2	T	C	15: 89,157,770 (GRCm38)	N1642S	possibly damaging	Het
Pnpla7	A	T	2: 25,011,564 (GRCm38)	D534V	probably damaging	Het
Ppp2r5c	A	T	12: 110,554,879 (GRCm38)	S279C	probably damaging	Het
Ptprq	T	A	10: 107,708,668 (GRCm38)	T334S	probably damaging	Het
Rab39	T	C	9: 53,686,521 (GRCm38)	D148G	probably benign	Het
Reep3	A	T	10: 67,034,653 (GRCm38)	F121L	probably benign	Het
Reln	G	T	5: 21,901,663 (GRCm38)	C3236*	probably null	Het
Rrp8	C	T	7: 105,734,809 (GRCm38)	C162Y	probably damaging	Het
Scrib	C	A	15: 76,064,986 (GRCm38)	Q399H	possibly damaging	Het
Sertad1	T	A	7: 27,489,799 (GRCm38)	Y182N	possibly damaging	Het
Sfrp5	A	G	19: 42,201,824 (GRCm38)	V63A	possibly damaging	Het
Slc46a1	T	C	11: 78,467,159 (GRCm38)	M346T	probably benign	Het
Sycp2	A	G	2: 178,363,416 (GRCm38)	L886S	probably damaging	Het
Teddm1b	A	T	1: 153,874,759 (GRCm38)	I105F	probably benign	Het
Thumpd1	A	T	7: 119,720,605 (GRCm38)	I46N	possibly damaging	Het
Tmod4	A	G	3: 95,127,853 (GRCm38)	N223S	probably damaging	Het
Tnfrsf10b	G	C	14: 69,773,401 (GRCm38)	C85S	probably damaging	Het
Tox	T	C	4: 6,741,536 (GRCm38)	Q148R	probably benign	Het
Tox	T	C	4: 6,697,439 (GRCm38)	T455A	probably benign	Het
Trp53bp1	A	C	2: 121,269,945 (GRCm38)	S109R	probably damaging	Het
Tshz2	A	T	2: 169,884,968 (GRCm38)	T26S	probably benign	Het
Tubb4a	T	A	17: 57,081,016 (GRCm38)	N337Y	probably damaging	Het
Tubb6	T	A	18: 67,401,388 (GRCm38)	V119E	probably damaging	Het
Unk	C	T	11: 116,054,946 (GRCm38)	T481I	probably benign	Het
Vmn2r112	C	T	17: 22,601,278 (GRCm38)	T44I	probably benign	Het
Vstm5	G	A	9: 15,257,533 (GRCm38)	G131D	probably damaging	Het
Wscd1	T	C	11: 71,759,883 (GRCm38)	L12P	probably damaging	Het
Zfp369	T	A	13: 65,296,230 (GRCm38)	S396T	possibly damaging	Het
Zfp932	T	A	5: 110,009,343 (GRCm38)	C302*	probably null	Het
Zfp972	A	T	2: 177,906,935 (GRCm38)		probably null	Het
Zfyve21	C	A	12: 111,827,594 (GRCm38)	A212E	probably benign	Het

Other mutations in Fat1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Fat1	APN	8	45,024,602 (GRCm38)	missense	possibly damaging	0.93
IGL00157:Fat1	APN	8	44,951,670 (GRCm38)	missense	possibly damaging	0.96
IGL00481:Fat1	APN	8	45,050,940 (GRCm38)	missense	probably benign	0.18
IGL00983:Fat1	APN	8	45,033,390 (GRCm38)	missense	probably damaging	1.00
IGL01089:Fat1	APN	8	45,017,857 (GRCm38)	missense	probably damaging	1.00
IGL01135:Fat1	APN	8	45,024,840 (GRCm38)	missense	probably damaging	1.00
IGL01143:Fat1	APN	8	45,035,532 (GRCm38)	missense	possibly damaging	0.72
IGL01155:Fat1	APN	8	45,023,949 (GRCm38)	missense	probably damaging	1.00
IGL01376:Fat1	APN	8	45,026,841 (GRCm38)	missense	probably benign	0.00
IGL01411:Fat1	APN	8	45,026,800 (GRCm38)	missense	probably damaging	1.00
IGL01443:Fat1	APN	8	45,040,576 (GRCm38)	missense	probably damaging	1.00
IGL01453:Fat1	APN	8	45,051,270 (GRCm38)	missense	probably damaging	1.00
IGL01606:Fat1	APN	8	45,023,049 (GRCm38)	missense	probably benign	0.26
IGL01622:Fat1	APN	8	45,029,555 (GRCm38)	missense	possibly damaging	0.64
IGL01623:Fat1	APN	8	45,029,555 (GRCm38)	missense	possibly damaging	0.64
IGL01672:Fat1	APN	8	45,040,700 (GRCm38)	missense	probably benign	0.05
IGL01735:Fat1	APN	8	45,036,239 (GRCm38)	missense	probably benign	0.07
IGL01793:Fat1	APN	8	44,989,112 (GRCm38)	missense	probably benign
IGL01820:Fat1	APN	8	45,010,502 (GRCm38)	missense	probably damaging	1.00
IGL01969:Fat1	APN	8	44,952,599 (GRCm38)	missense	probably damaging	0.98
IGL02012:Fat1	APN	8	45,027,540 (GRCm38)	missense	possibly damaging	0.95
IGL02227:Fat1	APN	8	45,023,659 (GRCm38)	missense	probably damaging	1.00
IGL02256:Fat1	APN	8	44,950,332 (GRCm38)	missense	probably damaging	1.00
IGL02273:Fat1	APN	8	44,950,331 (GRCm38)	missense	probably damaging	1.00
IGL02317:Fat1	APN	8	45,025,818 (GRCm38)	missense	probably benign	0.33
IGL02324:Fat1	APN	8	45,040,556 (GRCm38)	missense	probably damaging	1.00
IGL02336:Fat1	APN	8	44,951,583 (GRCm38)	missense	probably benign	0.16
IGL02442:Fat1	APN	8	44,950,323 (GRCm38)	missense	probably benign	0.02
IGL02486:Fat1	APN	8	45,025,072 (GRCm38)	missense	probably benign	0.16
IGL02551:Fat1	APN	8	45,051,398 (GRCm38)	missense	probably damaging	1.00
IGL02617:Fat1	APN	8	45,035,591 (GRCm38)	missense	probably benign	0.31
IGL02698:Fat1	APN	8	45,023,164 (GRCm38)	missense	probably benign
IGL02885:Fat1	APN	8	44,989,167 (GRCm38)	missense	probably benign	0.01
IGL02904:Fat1	APN	8	45,040,682 (GRCm38)	missense	probably damaging	1.00
IGL02953:Fat1	APN	8	45,024,314 (GRCm38)	missense	probably damaging	1.00
IGL03108:Fat1	APN	8	45,023,614 (GRCm38)	missense	probably damaging	1.00
IGL03153:Fat1	APN	8	45,030,123 (GRCm38)	missense	possibly damaging	0.83
IGL03183:Fat1	APN	8	44,950,586 (GRCm38)	missense	probably damaging	0.99
IGL03327:Fat1	APN	8	44,950,468 (GRCm38)	missense	probably damaging	1.00
IGL03405:Fat1	APN	8	45,025,241 (GRCm38)	missense	probably damaging	1.00
Laggardly	UTSW	8	45,044,464 (GRCm38)	missense	probably damaging	1.00
R2257_fat1_465	UTSW	8	44,950,371 (GRCm38)	missense	probably damaging	1.00
Shrinkage	UTSW	8	45,018,037 (GRCm38)	missense	probably damaging	1.00
F5493:Fat1	UTSW	8	45,025,480 (GRCm38)	missense	probably damaging	0.99
G1citation:Fat1	UTSW	8	45,026,404 (GRCm38)	missense	probably damaging	1.00
I2289:Fat1	UTSW	8	45,024,996 (GRCm38)	missense	probably benign	0.01
IGL02837:Fat1	UTSW	8	45,017,434 (GRCm38)	missense	probably benign	0.00
PIT4283001:Fat1	UTSW	8	45,037,207 (GRCm38)	missense	probably damaging	1.00
PIT4283001:Fat1	UTSW	8	45,029,540 (GRCm38)	missense	probably damaging	1.00
PIT4576001:Fat1	UTSW	8	45,024,645 (GRCm38)	missense	probably damaging	1.00
R0040:Fat1	UTSW	8	45,026,404 (GRCm38)	missense	probably damaging	1.00
R0040:Fat1	UTSW	8	45,026,404 (GRCm38)	missense	probably damaging	1.00
R0078:Fat1	UTSW	8	44,953,299 (GRCm38)	missense	probably damaging	1.00
R0197:Fat1	UTSW	8	45,026,553 (GRCm38)	missense	probably benign	0.00
R0328:Fat1	UTSW	8	45,023,790 (GRCm38)	missense	probably benign	0.35
R0367:Fat1	UTSW	8	45,024,313 (GRCm38)	missense	probably damaging	1.00
R0371:Fat1	UTSW	8	44,951,892 (GRCm38)	missense	probably damaging	1.00
R0380:Fat1	UTSW	8	45,010,123 (GRCm38)	missense	probably damaging	0.97
R0389:Fat1	UTSW	8	44,950,348 (GRCm38)	missense	probably benign	0.00
R0433:Fat1	UTSW	8	45,024,649 (GRCm38)	missense	possibly damaging	0.51
R0456:Fat1	UTSW	8	45,029,534 (GRCm38)	missense	probably damaging	1.00
R0494:Fat1	UTSW	8	44,950,542 (GRCm38)	missense	probably damaging	1.00
R0506:Fat1	UTSW	8	45,022,951 (GRCm38)	missense	probably damaging	0.99
R0512:Fat1	UTSW	8	44,951,332 (GRCm38)	nonsense	probably null
R0624:Fat1	UTSW	8	45,051,168 (GRCm38)	missense	possibly damaging	0.46
R0701:Fat1	UTSW	8	45,026,553 (GRCm38)	missense	probably benign	0.00
R0723:Fat1	UTSW	8	45,026,749 (GRCm38)	missense	probably damaging	1.00
R0787:Fat1	UTSW	8	45,040,555 (GRCm38)	missense	probably damaging	1.00
R0788:Fat1	UTSW	8	45,023,983 (GRCm38)	missense	probably benign	0.27
R0862:Fat1	UTSW	8	45,018,037 (GRCm38)	missense	probably damaging	1.00
R0864:Fat1	UTSW	8	45,018,037 (GRCm38)	missense	probably damaging	1.00
R0907:Fat1	UTSW	8	45,026,598 (GRCm38)	missense	probably benign	0.08
R0962:Fat1	UTSW	8	45,033,326 (GRCm38)	splice site	probably benign
R1051:Fat1	UTSW	8	45,044,506 (GRCm38)	missense	probably damaging	1.00
R1156:Fat1	UTSW	8	45,039,890 (GRCm38)	missense	possibly damaging	0.94
R1237:Fat1	UTSW	8	45,044,279 (GRCm38)	missense	probably damaging	1.00
R1468:Fat1	UTSW	8	45,010,545 (GRCm38)	missense	probably damaging	1.00
R1468:Fat1	UTSW	8	45,010,545 (GRCm38)	missense	probably damaging	1.00
R1478:Fat1	UTSW	8	45,025,622 (GRCm38)	missense	probably damaging	0.99
R1482:Fat1	UTSW	8	44,953,244 (GRCm38)	missense	probably benign	0.04
R1496:Fat1	UTSW	8	45,033,390 (GRCm38)	missense	probably damaging	1.00
R1498:Fat1	UTSW	8	45,025,484 (GRCm38)	nonsense	probably null
R1508:Fat1	UTSW	8	45,026,862 (GRCm38)	missense	probably benign	0.01
R1577:Fat1	UTSW	8	45,023,383 (GRCm38)	missense	probably benign	0.30
R1646:Fat1	UTSW	8	45,018,042 (GRCm38)	missense	probably damaging	1.00
R1652:Fat1	UTSW	8	45,025,178 (GRCm38)	nonsense	probably null
R1656:Fat1	UTSW	8	45,025,530 (GRCm38)	nonsense	probably null
R1662:Fat1	UTSW	8	44,953,164 (GRCm38)	missense	probably benign	0.20
R1672:Fat1	UTSW	8	45,036,835 (GRCm38)	missense	probably damaging	1.00
R1704:Fat1	UTSW	8	45,025,576 (GRCm38)	missense	probably damaging	1.00
R1708:Fat1	UTSW	8	45,024,792 (GRCm38)	missense	probably damaging	1.00
R1710:Fat1	UTSW	8	45,010,482 (GRCm38)	missense	probably benign	0.00
R1812:Fat1	UTSW	8	45,036,803 (GRCm38)	missense	probably damaging	1.00
R1872:Fat1	UTSW	8	45,038,349 (GRCm38)	missense	probably damaging	1.00
R1872:Fat1	UTSW	8	44,953,304 (GRCm38)	missense	probably benign	0.01
R1883:Fat1	UTSW	8	45,051,147 (GRCm38)	missense	probably benign	0.17
R1893:Fat1	UTSW	8	45,023,856 (GRCm38)	missense	probably damaging	1.00
R1930:Fat1	UTSW	8	45,044,228 (GRCm38)	missense	possibly damaging	0.91
R1931:Fat1	UTSW	8	45,044,228 (GRCm38)	missense	possibly damaging	0.91
R1952:Fat1	UTSW	8	45,033,926 (GRCm38)	missense	probably benign	0.00
R1957:Fat1	UTSW	8	45,040,682 (GRCm38)	missense	probably damaging	1.00
R1999:Fat1	UTSW	8	44,952,393 (GRCm38)	missense	probably damaging	0.96
R2019:Fat1	UTSW	8	45,023,746 (GRCm38)	missense	probably damaging	1.00
R2062:Fat1	UTSW	8	45,026,704 (GRCm38)	missense	probably damaging	1.00
R2062:Fat1	UTSW	8	45,024,332 (GRCm38)	missense	probably damaging	1.00
R2117:Fat1	UTSW	8	45,037,463 (GRCm38)	missense	probably benign	0.33
R2196:Fat1	UTSW	8	45,024,646 (GRCm38)	missense	probably damaging	1.00
R2204:Fat1	UTSW	8	45,023,700 (GRCm38)	missense	probably damaging	1.00
R2256:Fat1	UTSW	8	44,950,371 (GRCm38)	missense	probably damaging	1.00
R2257:Fat1	UTSW	8	44,950,371 (GRCm38)	missense	probably damaging	1.00
R2409:Fat1	UTSW	8	45,040,530 (GRCm38)	splice site	probably benign
R2416:Fat1	UTSW	8	45,026,383 (GRCm38)	missense	probably damaging	1.00
R3021:Fat1	UTSW	8	45,044,011 (GRCm38)	missense	probably damaging	1.00
R3108:Fat1	UTSW	8	45,045,173 (GRCm38)	splice site	probably null
R3109:Fat1	UTSW	8	45,045,173 (GRCm38)	splice site	probably null
R3196:Fat1	UTSW	8	44,951,868 (GRCm38)	missense	probably benign	0.00
R3683:Fat1	UTSW	8	45,017,938 (GRCm38)	missense	probably benign
R3732:Fat1	UTSW	8	44,953,269 (GRCm38)	missense	possibly damaging	0.85
R3732:Fat1	UTSW	8	44,953,269 (GRCm38)	missense	possibly damaging	0.85
R3733:Fat1	UTSW	8	44,953,269 (GRCm38)	missense	possibly damaging	0.85
R3753:Fat1	UTSW	8	45,025,479 (GRCm38)	missense	probably damaging	0.97
R3905:Fat1	UTSW	8	45,023,035 (GRCm38)	missense	probably benign	0.00
R3907:Fat1	UTSW	8	45,023,035 (GRCm38)	missense	probably benign	0.00
R3908:Fat1	UTSW	8	45,023,035 (GRCm38)	missense	probably benign	0.00
R4060:Fat1	UTSW	8	45,025,481 (GRCm38)	missense	probably benign	0.09
R4061:Fat1	UTSW	8	45,025,481 (GRCm38)	missense	probably benign	0.09
R4062:Fat1	UTSW	8	45,025,481 (GRCm38)	missense	probably benign	0.09
R4063:Fat1	UTSW	8	45,025,481 (GRCm38)	missense	probably benign	0.09
R4078:Fat1	UTSW	8	44,989,122 (GRCm38)	missense	probably damaging	0.99
R4105:Fat1	UTSW	8	45,036,851 (GRCm38)	missense	probably damaging	1.00
R4118:Fat1	UTSW	8	45,050,944 (GRCm38)	missense	probably damaging	1.00
R4118:Fat1	UTSW	8	45,010,437 (GRCm38)	missense	probably damaging	1.00
R4161:Fat1	UTSW	8	45,036,787 (GRCm38)	missense	probably benign	0.00
R4364:Fat1	UTSW	8	44,952,962 (GRCm38)	missense	probably benign	0.01
R4394:Fat1	UTSW	8	44,952,346 (GRCm38)	missense	probably damaging	0.98
R4395:Fat1	UTSW	8	44,952,346 (GRCm38)	missense	probably damaging	0.98
R4396:Fat1	UTSW	8	44,952,346 (GRCm38)	missense	probably damaging	0.98
R4412:Fat1	UTSW	8	45,023,599 (GRCm38)	missense	probably damaging	0.99
R4542:Fat1	UTSW	8	45,041,894 (GRCm38)	missense	probably damaging	1.00
R4591:Fat1	UTSW	8	45,026,242 (GRCm38)	missense	probably benign
R4606:Fat1	UTSW	8	44,950,683 (GRCm38)	missense	possibly damaging	0.47
R4612:Fat1	UTSW	8	45,025,147 (GRCm38)	missense	probably damaging	1.00
R4730:Fat1	UTSW	8	45,033,477 (GRCm38)	missense	probably damaging	1.00
R4778:Fat1	UTSW	8	45,038,326 (GRCm38)	missense	probably benign	0.04
R4824:Fat1	UTSW	8	44,989,114 (GRCm38)	missense	probably damaging	1.00
R4829:Fat1	UTSW	8	45,036,162 (GRCm38)	missense	probably damaging	1.00
R4832:Fat1	UTSW	8	45,013,065 (GRCm38)	missense	possibly damaging	0.95
R4849:Fat1	UTSW	8	45,012,970 (GRCm38)	missense	probably benign	0.15
R4896:Fat1	UTSW	8	44,951,280 (GRCm38)	missense	possibly damaging	0.68
R4927:Fat1	UTSW	8	45,022,963 (GRCm38)	missense	probably damaging	0.96
R4941:Fat1	UTSW	8	45,036,275 (GRCm38)	missense	probably benign	0.00
R5011:Fat1	UTSW	8	45,031,263 (GRCm38)	critical splice acceptor site	probably null
R5040:Fat1	UTSW	8	45,023,380 (GRCm38)	missense	probably damaging	1.00
R5112:Fat1	UTSW	8	45,024,282 (GRCm38)	missense	probably damaging	1.00
R5151:Fat1	UTSW	8	44,951,814 (GRCm38)	missense	possibly damaging	0.74
R5161:Fat1	UTSW	8	44,952,512 (GRCm38)	missense	probably benign	0.00
R5162:Fat1	UTSW	8	45,025,809 (GRCm38)	missense	probably benign	0.02
R5353:Fat1	UTSW	8	45,036,131 (GRCm38)	missense	probably benign	0.13
R5425:Fat1	UTSW	8	45,025,885 (GRCm38)	missense	possibly damaging	0.64
R5458:Fat1	UTSW	8	45,013,053 (GRCm38)	missense	probably damaging	1.00
R5479:Fat1	UTSW	8	45,036,875 (GRCm38)	missense	possibly damaging	0.88
R5543:Fat1	UTSW	8	45,023,479 (GRCm38)	missense	probably damaging	0.99
R5569:Fat1	UTSW	8	45,039,836 (GRCm38)	missense	probably damaging	0.98
R5610:Fat1	UTSW	8	44,953,072 (GRCm38)	nonsense	probably null
R5734:Fat1	UTSW	8	45,051,209 (GRCm38)	missense	probably damaging	0.99
R5832:Fat1	UTSW	8	45,017,423 (GRCm38)	missense	possibly damaging	0.65
R5860:Fat1	UTSW	8	45,051,129 (GRCm38)	missense	probably benign
R5886:Fat1	UTSW	8	45,033,395 (GRCm38)	missense	probably damaging	1.00
R5886:Fat1	UTSW	8	45,027,681 (GRCm38)	critical splice donor site	probably null
R5919:Fat1	UTSW	8	45,026,873 (GRCm38)	critical splice donor site	probably null
R5930:Fat1	UTSW	8	45,044,036 (GRCm38)	missense	probably benign	0.10
R5960:Fat1	UTSW	8	45,033,368 (GRCm38)	missense	probably damaging	1.00
R5988:Fat1	UTSW	8	45,029,456 (GRCm38)	missense	probably benign	0.00
R6166:Fat1	UTSW	8	44,952,485 (GRCm38)	missense	probably damaging	1.00
R6184:Fat1	UTSW	8	44,953,392 (GRCm38)	missense	probably benign	0.00
R6208:Fat1	UTSW	8	45,027,613 (GRCm38)	missense	probably damaging	0.99
R6391:Fat1	UTSW	8	44,952,342 (GRCm38)	missense	possibly damaging	0.69
R6701:Fat1	UTSW	8	44,950,681 (GRCm38)	missense	probably damaging	1.00
R6702:Fat1	UTSW	8	44,953,046 (GRCm38)	missense	probably benign	0.28
R6703:Fat1	UTSW	8	44,953,046 (GRCm38)	missense	probably benign	0.28
R6704:Fat1	UTSW	8	45,024,373 (GRCm38)	missense	probably damaging	1.00
R6822:Fat1	UTSW	8	45,026,404 (GRCm38)	missense	probably damaging	1.00
R6852:Fat1	UTSW	8	45,035,598 (GRCm38)	missense	possibly damaging	0.46
R6863:Fat1	UTSW	8	45,044,464 (GRCm38)	missense	probably damaging	1.00
R6885:Fat1	UTSW	8	44,952,452 (GRCm38)	missense	possibly damaging	0.94
R6912:Fat1	UTSW	8	45,051,023 (GRCm38)	missense	probably benign	0.00
R6927:Fat1	UTSW	8	45,024,495 (GRCm38)	missense	probably benign	0.41
R6964:Fat1	UTSW	8	45,043,945 (GRCm38)	missense	probably damaging	1.00
R7010:Fat1	UTSW	8	44,953,349 (GRCm38)	nonsense	probably null
R7062:Fat1	UTSW	8	44,950,216 (GRCm38)	start codon destroyed	probably null	0.99
R7063:Fat1	UTSW	8	45,040,775 (GRCm38)	missense	probably benign	0.09
R7071:Fat1	UTSW	8	44,989,108 (GRCm38)	missense	possibly damaging	0.67
R7117:Fat1	UTSW	8	45,031,468 (GRCm38)	missense	probably damaging	0.98
R7146:Fat1	UTSW	8	44,950,925 (GRCm38)	missense	probably benign
R7210:Fat1	UTSW	8	45,023,503 (GRCm38)	missense	probably damaging	1.00
R7227:Fat1	UTSW	8	45,010,609 (GRCm38)	missense	probably benign	0.08
R7270:Fat1	UTSW	8	45,037,438 (GRCm38)	missense	probably damaging	1.00
R7373:Fat1	UTSW	8	45,026,665 (GRCm38)	missense	probably damaging	1.00
R7390:Fat1	UTSW	8	44,952,474 (GRCm38)	missense	possibly damaging	0.81
R7465:Fat1	UTSW	8	45,044,152 (GRCm38)	missense	probably benign	0.35
R7476:Fat1	UTSW	8	45,031,274 (GRCm38)	missense	probably benign	0.01
R7483:Fat1	UTSW	8	45,023,160 (GRCm38)	missense	probably benign	0.13
R7484:Fat1	UTSW	8	45,036,184 (GRCm38)	missense	probably damaging	1.00
R7526:Fat1	UTSW	8	45,023,427 (GRCm38)	missense	probably damaging	1.00
R7549:Fat1	UTSW	8	44,988,994 (GRCm38)	missense	probably benign	0.01
R7554:Fat1	UTSW	8	45,037,165 (GRCm38)	missense	possibly damaging	0.88
R7620:Fat1	UTSW	8	45,009,850 (GRCm38)	missense	possibly damaging	0.95
R7652:Fat1	UTSW	8	44,953,299 (GRCm38)	missense	probably damaging	1.00
R7694:Fat1	UTSW	8	44,988,930 (GRCm38)	critical splice acceptor site	probably null
R7746:Fat1	UTSW	8	44,951,633 (GRCm38)	missense	probably damaging	0.96
R7762:Fat1	UTSW	8	45,037,337 (GRCm38)	missense	probably damaging	0.99
R7762:Fat1	UTSW	8	45,023,322 (GRCm38)	missense	probably damaging	1.00
R7782:Fat1	UTSW	8	44,950,911 (GRCm38)	missense	probably damaging	1.00
R7801:Fat1	UTSW	8	45,042,223 (GRCm38)	missense	probably damaging	1.00
R7807:Fat1	UTSW	8	45,041,973 (GRCm38)	missense	probably damaging	1.00
R7821:Fat1	UTSW	8	44,950,224 (GRCm38)	missense	probably benign
R7869:Fat1	UTSW	8	45,051,222 (GRCm38)	missense	probably benign	0.02
R8034:Fat1	UTSW	8	44,951,691 (GRCm38)	missense	probably benign	0.28
R8094:Fat1	UTSW	8	44,952,702 (GRCm38)	missense	probably damaging	0.98
R8111:Fat1	UTSW	8	45,026,058 (GRCm38)	missense	possibly damaging	0.94
R8220:Fat1	UTSW	8	45,039,956 (GRCm38)	missense	probably null
R8221:Fat1	UTSW	8	44,953,353 (GRCm38)	missense
R8233:Fat1	UTSW	8	44,952,018 (GRCm38)	missense
R8250:Fat1	UTSW	8	44,953,299 (GRCm38)	missense	probably damaging	1.00
R8279:Fat1	UTSW	8	45,030,347 (GRCm38)	critical splice donor site	probably null
R8726:Fat1	UTSW	8	45,024,169 (GRCm38)	missense	probably benign	0.23
R8875:Fat1	UTSW	8	45,040,563 (GRCm38)	missense	probably damaging	1.00
R8937:Fat1	UTSW	8	45,030,313 (GRCm38)	missense	probably damaging	1.00
R8950:Fat1	UTSW	8	45,023,121 (GRCm38)	missense	probably damaging	1.00
R8971:Fat1	UTSW	8	45,042,294 (GRCm38)	missense	probably damaging	1.00
R8976:Fat1	UTSW	8	45,031,295 (GRCm38)	missense	probably benign	0.02
R9000:Fat1	UTSW	8	45,044,550 (GRCm38)	nonsense	probably null
R9032:Fat1	UTSW	8	45,039,857 (GRCm38)	missense	probably benign	0.01
R9076:Fat1	UTSW	8	45,039,901 (GRCm38)	missense	probably damaging	1.00
R9083:Fat1	UTSW	8	45,038,299 (GRCm38)	missense	probably benign	0.00
R9083:Fat1	UTSW	8	45,013,090 (GRCm38)	missense	possibly damaging	0.76
R9103:Fat1	UTSW	8	44,951,813 (GRCm38)	missense	probably benign	0.38
R9124:Fat1	UTSW	8	45,025,027 (GRCm38)	missense	possibly damaging	0.48
R9124:Fat1	UTSW	8	44,950,326 (GRCm38)	missense	probably benign
R9128:Fat1	UTSW	8	45,009,841 (GRCm38)	missense	probably benign	0.14
R9148:Fat1	UTSW	8	44,952,645 (GRCm38)	missense	possibly damaging	0.81
R9162:Fat1	UTSW	8	44,951,315 (GRCm38)	missense	probably damaging	1.00
R9209:Fat1	UTSW	8	44,951,754 (GRCm38)	missense	possibly damaging	0.80
R9276:Fat1	UTSW	8	45,035,477 (GRCm38)	missense	probably damaging	0.99
R9303:Fat1	UTSW	8	45,010,461 (GRCm38)	missense	probably damaging	1.00
R9319:Fat1	UTSW	8	44,953,023 (GRCm38)	missense	probably damaging	1.00
R9392:Fat1	UTSW	8	45,023,191 (GRCm38)	missense	probably damaging	1.00
R9616:Fat1	UTSW	8	44,953,038 (GRCm38)	missense	probably damaging	0.99
R9712:Fat1	UTSW	8	45,017,380 (GRCm38)	missense	probably benign	0.05
R9756:Fat1	UTSW	8	45,043,937 (GRCm38)	missense	probably damaging	0.96
RF001:Fat1	UTSW	8	44,988,966 (GRCm38)	missense	probably benign	0.00
X0064:Fat1	UTSW	8	45,025,734 (GRCm38)	missense	possibly damaging	0.58
Z1088:Fat1	UTSW	8	45,023,807 (GRCm38)	missense	possibly damaging	0.88
Z1176:Fat1	UTSW	8	45,036,838 (GRCm38)	missense	probably damaging	1.00
Z1176:Fat1	UTSW	8	45,023,596 (GRCm38)	missense	possibly damaging	0.65
Z1176:Fat1	UTSW	8	44,950,598 (GRCm38)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- CTGGACGTAATTCTGTCTGTCC -3'
(R):5'- AGGACAGTGTGAGTGCTCTAG -3'

Sequencing Primer
(F):5'- GTCCATACAGGGGCCATATTTATC -3'
(R):5'- TGTCCTCTGACTGAAAAACTGC -3'

Posted On

2018-04-27