Mutagenetix > Incidental Mutation

Incidental Mutation 'R6338:Cdh23'

514338

Institutional Source

Beutler Lab

Gene Symbol

Cdh23

Ensembl Gene

ENSMUSG00000012819

Gene Name

cadherin 23 (otocadherin)

Synonyms

nmf252, bob, ahl, mdfw, 4930542A03Rik, sals, nmf112, nmf181, USH1D

MMRRC Submission

044492-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.608) question?

Stock #

R6338 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

60302748-60696490 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 60413151 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 882 (D882G)

Ref Sequence

ENSEMBL: ENSMUSP00000101103 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073242] [ENSMUST00000105461] [ENSMUST00000105462] [ENSMUST00000105463] [ENSMUST00000105464]

AlphaFold

no structure available at present

PDB Structure

Crystal structure of mouse cadherin-23 EC1 [X-RAY DIFFRACTION]
CRYSTAL STRUCTURE OF MOUSE CADHERIN-23 EC1-2 [X-RAY DIFFRACTION]
CRYSTAL STRUCTURE OF NONSYNDROMIC DEAFNESS (DFNB12) ASSOCIATED MUTANT D124G OF MOUSE CADHERIN-23 EC1-2 [X-RAY DIFFRACTION]
CRYSTAL STRUCTURE OF MOUSE CADHERIN-23 EC1-2 (ALL CATION BINDING SITES OCCUPIED BY CALCIUM) [X-RAY DIFFRACTION]
Structure of the N-terminus of Cadherin 23 [X-RAY DIFFRACTION]
CRYSTAL STRUCTURE OF MOUSE CADHERIN-23 EC1-2 AND PROTOCADHERIN-15 EC1- 2 FORM I [X-RAY DIFFRACTION]
CRYSTAL STRUCTURE OF MOUSE CADHERIN-23 EC1-2 AND PROTOCADHERIN-15 EC1- 2 FORM II [X-RAY DIFFRACTION]
CRYSTAL STRUCTURE OF DEAFNESS ASSOCIATED MUTANT MOUSE CADHERIN-23 EC1- 2D124G AND PROTOCADHERIN-15 EC1-2 FORM I [X-RAY DIFFRACTION]
CRYSTAL STRUCTURE OF DEAFNESS ASSOCIATED MUTANT MOUSE CADHERIN-23 EC1- 2S70P AND PROTOCADHERIN-15 EC1-2 FORM I [X-RAY DIFFRACTION]
CRYSTAL STRUCTURE OF MOUSE CADHERIN-23 EC1-2 AND PROTOCADHERIN-15 EC1- 2, FORM I 2.2A. [X-RAY DIFFRACTION]

Predicted Effect

probably damaging
Transcript: ENSMUST00000073242
AA Change: D882G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000072973
Gene: ENSMUSG00000012819
AA Change: D882G

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
CA	55	130	5.15e-13	SMART
CA	154	234	3.19e-18	SMART
CA	258	346	2.03e-11	SMART
CA	371	458	8.11e-11	SMART
CA	482	559	1.04e-22	SMART
CA	583	669	3.55e-25	SMART
CA	693	776	2.04e-25	SMART
CA	800	888	5.03e-16	SMART
CA	912	993	1.05e-27	SMART
CA	1017	1100	1.99e-19	SMART
CA	1124	1206	6.94e-19	SMART
CA	1231	1311	1.99e-19	SMART
CA	1335	1415	1.21e-18	SMART
CA	1440	1524	2.38e-26	SMART
CA	1549	1631	6.27e-26	SMART
CA	1656	1741	6.99e-24	SMART
CA	1765	1848	3.49e-24	SMART
CA	1872	1956	2.78e-18	SMART
CA	1984	2066	5.6e-14	SMART
CA	2090	2171	2.59e-27	SMART
CA	2195	2290	2.87e-11	SMART
CA	2317	2399	1.01e-20	SMART
CA	2423	2506	1.09e-25	SMART
CA	2530	2608	7.91e-23	SMART
CA	2634	2719	1.06e-23	SMART
CA	2750	2843	2e-10	SMART
Blast:CA	2867	2956	4e-51	BLAST
transmembrane domain	3067	3089	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000105461
AA Change: D882G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000101101
Gene: ENSMUSG00000012819
AA Change: D882G

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
CA	55	130	5.15e-13	SMART
CA	154	234	3.19e-18	SMART
CA	258	346	2.03e-11	SMART
CA	371	458	1.25e-11	SMART
CA	482	559	1.04e-22	SMART
CA	583	669	3.55e-25	SMART
CA	693	776	2.04e-25	SMART
CA	800	888	5.03e-16	SMART
CA	912	993	1.05e-27	SMART
CA	1017	1100	1.99e-19	SMART
CA	1124	1206	6.94e-19	SMART
CA	1231	1311	1.99e-19	SMART
CA	1335	1416	5.26e-19	SMART
CA	1441	1525	2.38e-26	SMART
CA	1550	1632	6.27e-26	SMART
CA	1657	1742	6.99e-24	SMART
CA	1766	1849	3.49e-24	SMART
CA	1873	1957	2.78e-18	SMART
CA	1985	2067	5.6e-14	SMART
CA	2091	2172	2.59e-27	SMART
CA	2196	2291	2.87e-11	SMART
CA	2318	2400	1.01e-20	SMART
CA	2424	2507	1.09e-25	SMART
CA	2531	2609	7.91e-23	SMART
CA	2635	2720	1.06e-23	SMART
CA	2751	2844	2e-10	SMART
Blast:CA	2868	2957	4e-51	BLAST
transmembrane domain	3068	3090	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000105462
AA Change: D885G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000101102
Gene: ENSMUSG00000012819
AA Change: D885G

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
CA	55	130	5.15e-13	SMART
CA	154	234	3.19e-18	SMART
CA	261	349	2.03e-11	SMART
CA	374	461	8.11e-11	SMART
CA	485	562	1.04e-22	SMART
CA	586	672	3.55e-25	SMART
CA	696	779	2.04e-25	SMART
CA	803	891	5.03e-16	SMART
CA	915	996	1.05e-27	SMART
CA	1020	1103	1.99e-19	SMART
CA	1127	1209	6.94e-19	SMART
CA	1234	1314	1.99e-19	SMART
CA	1338	1418	1.21e-18	SMART
CA	1443	1527	2.38e-26	SMART
CA	1552	1634	6.27e-26	SMART
CA	1659	1744	6.99e-24	SMART
CA	1768	1851	3.49e-24	SMART
CA	1875	1959	2.78e-18	SMART
CA	1987	2069	5.6e-14	SMART
CA	2093	2174	2.59e-27	SMART
CA	2198	2293	2.87e-11	SMART
CA	2320	2402	1.01e-20	SMART
CA	2426	2509	1.09e-25	SMART
CA	2533	2611	7.91e-23	SMART
CA	2637	2722	1.06e-23	SMART
CA	2753	2846	2e-10	SMART
Blast:CA	2870	2959	4e-51	BLAST
transmembrane domain	3070	3092	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000105463
AA Change: D882G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000101103
Gene: ENSMUSG00000012819
AA Change: D882G

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
CA	55	130	5.15e-13	SMART
CA	154	234	3.19e-18	SMART
CA	258	346	2.03e-11	SMART
CA	371	458	1.25e-11	SMART
CA	482	559	1.04e-22	SMART
CA	583	669	3.55e-25	SMART
CA	693	776	2.04e-25	SMART
CA	800	888	5.03e-16	SMART
CA	912	993	1.05e-27	SMART
CA	1017	1100	1.99e-19	SMART
CA	1124	1206	6.94e-19	SMART
CA	1231	1311	1.99e-19	SMART
CA	1335	1416	5.26e-19	SMART
CA	1441	1525	2.38e-26	SMART
CA	1550	1632	6.27e-26	SMART
CA	1657	1742	6.99e-24	SMART
CA	1766	1849	3.49e-24	SMART
CA	1873	1957	2.78e-18	SMART
CA	1985	2067	5.6e-14	SMART
CA	2091	2172	2.59e-27	SMART
CA	2196	2291	2.87e-11	SMART
CA	2318	2400	1.01e-20	SMART
CA	2424	2507	1.09e-25	SMART
CA	2531	2609	7.91e-23	SMART
CA	2635	2720	1.06e-23	SMART
CA	2751	2844	2e-10	SMART
Blast:CA	2868	2957	4e-51	BLAST
transmembrane domain	3068	3090	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000105464
AA Change: D880G

PolyPhen 2 Score 0.845 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000101104
Gene: ENSMUSG00000012819
AA Change: D880G

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
CA	55	130	5.15e-13	SMART
CA	154	234	3.19e-18	SMART
CA	258	346	2.03e-11	SMART
CA	371	456	3.58e-12	SMART
CA	480	557	1.04e-22	SMART
CA	581	667	3.55e-25	SMART
CA	691	774	2.04e-25	SMART
CA	798	886	5.03e-16	SMART
CA	910	991	1.05e-27	SMART
CA	1015	1098	1.99e-19	SMART
CA	1122	1204	6.94e-19	SMART
CA	1229	1309	1.99e-19	SMART
CA	1333	1414	5.26e-19	SMART
CA	1439	1523	2.38e-26	SMART
CA	1548	1630	6.27e-26	SMART
CA	1655	1740	6.99e-24	SMART
CA	1764	1847	3.49e-24	SMART
CA	1871	1955	2.78e-18	SMART
CA	1983	2065	5.6e-14	SMART
CA	2089	2170	2.59e-27	SMART
CA	2194	2289	2.87e-11	SMART
CA	2316	2398	1.01e-20	SMART
CA	2422	2505	1.09e-25	SMART
CA	2529	2607	7.91e-23	SMART
CA	2633	2718	1.06e-23	SMART
CA	2749	2842	2e-10	SMART
Blast:CA	2866	2955	3e-51	BLAST
transmembrane domain	3066	3088	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000128249

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000135638

Coding Region Coverage

1x: 100.0%
3x: 99.7%
10x: 98.1%
20x: 93.8%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the cadherin superfamily, whose genes encode calcium dependent cell-cell adhesion glycoproteins. The encoded protein is thought to be involved in stereocilia organization and hair bundle formation. The gene is located in a region containing the human deafness loci DFNB12 and USH1D. Usher syndrome 1D and nonsyndromic autosomal recessive deafness DFNB12 are caused by allelic mutations of this cadherin-like gene. Upregulation of this gene may also be associated with breast cancer. Alternative splice variants encoding different isoforms have been described. [provided by RefSeq, May 2013]
PHENOTYPE: Mutant mice exhibit circling behavior, tilting of the head and are deaf. Mice homozygous for a targeted knock-out exhibit abnormal outer hair cells morphology. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acer3	T	C	7: 98,257,715 (GRCm38)	Y128C	probably damaging	Het
Adam30	T	G	3: 98,161,541 (GRCm38)	I102S	probably damaging	Het
Adcy8	A	T	15: 64,920,617 (GRCm38)	D163E	possibly damaging	Het
Agrn	C	T	4: 156,170,585 (GRCm38)	E1614K	probably benign	Het
Aldh16a1	A	T	7: 45,141,961 (GRCm38)	W107R	probably damaging	Het
Arfgef2	A	G	2: 166,845,570 (GRCm38)	D238G	probably damaging	Het
Arhgap11a	T	C	2: 113,833,725 (GRCm38)	S738G	probably benign	Het
Arid5b	C	A	10: 68,098,561 (GRCm38)	G504*	probably null	Het
Carmil3	T	C	14: 55,499,849 (GRCm38)	V763A	possibly damaging	Het
Cd209e	T	A	8: 3,849,154 (GRCm38)	D186V	probably damaging	Het
Cdh4	T	C	2: 179,890,812 (GRCm38)	V689A	probably damaging	Het
Cntn6	T	A	6: 104,726,139 (GRCm38)	V174E	probably damaging	Het
Col7a1	C	T	9: 108,956,633 (GRCm38)	T390M	unknown	Het
Crybg1	T	C	10: 43,992,509 (GRCm38)	D1017G	probably damaging	Het
Csmd1	T	G	8: 15,932,492 (GRCm38)	K2725T	possibly damaging	Het
Dnaaf2	T	C	12: 69,198,122 (GRCm38)	E55G	probably damaging	Het
Fam13a	A	G	6: 58,953,499 (GRCm38)	V476A	probably damaging	Het
Fem1b	A	T	9: 62,797,011 (GRCm38)	D322E	probably benign	Het
Frmpd1	G	A	4: 45,274,489 (GRCm38)	V466I	probably benign	Het
Gm11639	C	T	11: 104,843,208 (GRCm38)	R2027*	probably null	Het
Gm20671	A	T	5: 32,820,647 (GRCm38)	D1794E	probably damaging	Het
Gpatch2	G	T	1: 187,225,514 (GRCm38)	R22L	probably damaging	Het
Gtf2h1	A	G	7: 46,816,456 (GRCm38)	T450A	probably benign	Het
Kcnc2	G	C	10: 112,271,856 (GRCm38)	G51R	probably benign	Het
Krit1	T	G	5: 3,836,857 (GRCm38)	M702R	probably benign	Het
Krt34	T	C	11: 100,038,490 (GRCm38)	N298S	probably benign	Het
Lrrcc1	T	A	3: 14,547,316 (GRCm38)	N376K	possibly damaging	Het
Myo15a	A	G	11: 60,478,133 (GRCm38)	E573G	probably damaging	Het
Olfr1224-ps1	T	A	2: 89,156,371 (GRCm38)	K268I	probably damaging	Het
Olfr391-ps	A	T	11: 73,799,319 (GRCm38)	L146Q	possibly damaging	Het
Or11j4	T	A	14: 50,393,400 (GRCm38)	F215I	possibly damaging	Het
Or1af1	A	T	2: 37,219,822 (GRCm38)	D111V	probably damaging	Het
Or2y17	A	G	11: 49,340,867 (GRCm38)	S112G	probably benign	Het
Or56b2	T	A	7: 104,688,171 (GRCm38)	V52E	possibly damaging	Het
Phf20	A	T	2: 156,273,686 (GRCm38)	Q309L	possibly damaging	Het
Plcd1	G	A	9: 119,074,991 (GRCm38)	R292C	probably damaging	Het
Pold3	A	G	7: 100,088,105 (GRCm38)	V342A	possibly damaging	Het
Polr2a	A	T	11: 69,739,679 (GRCm38)		probably null	Het
Ptprcap	A	G	19: 4,156,224 (GRCm38)	E102G	probably benign	Het
Rab11fip5	T	C	6: 85,341,378 (GRCm38)	E843G	possibly damaging	Het
Rai14	T	C	15: 10,574,976 (GRCm38)	D632G	probably damaging	Het
Rnf149	A	T	1: 39,560,742 (GRCm38)	C268S	probably null	Het
Slc6a13	T	A	6: 121,334,839 (GRCm38)	F392I	probably damaging	Het
Slc7a11	C	T	3: 50,384,043 (GRCm38)		probably null	Het
Slf1	T	A	13: 77,084,462 (GRCm38)		probably null	Het
Stard9	G	A	2: 120,697,485 (GRCm38)	V1408I	probably benign	Het
Suclg1	T	C	6: 73,264,246 (GRCm38)	I183T	probably damaging	Het
Syne1	T	C	10: 5,255,475 (GRCm38)	E3497G	probably benign	Het
Tax1bp1	C	T	6: 52,729,376 (GRCm38)	R121*	probably null	Het
Tdpoz2	C	T	3: 93,652,336 (GRCm38)	V110I	probably benign	Het
Ubn2	A	G	6: 38,490,714 (GRCm38)	T788A	probably benign	Het
Unc13c	T	A	9: 73,734,447 (GRCm38)	I1255F	probably damaging	Het
Usp44	G	T	10: 93,846,513 (GRCm38)	R275I	probably damaging	Het
Uspl1	A	G	5: 149,215,034 (GRCm38)	N1015D	probably benign	Het
Wbp2nl	G	T	15: 82,299,045 (GRCm38)	W13C	possibly damaging	Het
Zc3h14	T	A	12: 98,758,590 (GRCm38)	D170E	possibly damaging	Het

Other mutations in Cdh23

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00229:Cdh23	APN	10	60,523,548 (GRCm38)	missense	probably benign	0.03
IGL00429:Cdh23	APN	10	60,421,141 (GRCm38)	missense	probably damaging	0.97
IGL01014:Cdh23	APN	10	60,307,522 (GRCm38)	missense	probably damaging	0.99
IGL01284:Cdh23	APN	10	60,466,097 (GRCm38)	missense	possibly damaging	0.95
IGL01305:Cdh23	APN	10	60,312,624 (GRCm38)	missense	probably damaging	1.00
IGL01367:Cdh23	APN	10	60,310,787 (GRCm38)	missense	probably damaging	1.00
IGL01396:Cdh23	APN	10	60,385,069 (GRCm38)	missense	possibly damaging	0.93
IGL01412:Cdh23	APN	10	60,314,694 (GRCm38)	missense	probably damaging	1.00
IGL01461:Cdh23	APN	10	60,409,147 (GRCm38)	missense	possibly damaging	0.53
IGL01469:Cdh23	APN	10	60,597,725 (GRCm38)	missense	probably benign	0.03
IGL01695:Cdh23	APN	10	60,331,833 (GRCm38)	missense	probably benign	0.20
IGL01734:Cdh23	APN	10	60,303,513 (GRCm38)	missense	probably benign
IGL01767:Cdh23	APN	10	60,315,724 (GRCm38)	missense	probably damaging	1.00
IGL01796:Cdh23	APN	10	60,311,137 (GRCm38)	missense	probably benign	0.31
IGL01843:Cdh23	APN	10	60,419,819 (GRCm38)	splice site	probably null
IGL02025:Cdh23	APN	10	60,385,143 (GRCm38)	missense	probably damaging	1.00
IGL02071:Cdh23	APN	10	60,523,560 (GRCm38)	missense	possibly damaging	0.93
IGL02160:Cdh23	APN	10	60,597,765 (GRCm38)	splice site	probably benign
IGL02175:Cdh23	APN	10	60,331,308 (GRCm38)	missense	possibly damaging	0.92
IGL02220:Cdh23	APN	10	60,305,124 (GRCm38)	missense	probably damaging	1.00
IGL02302:Cdh23	APN	10	60,323,523 (GRCm38)	missense	possibly damaging	0.87
IGL02331:Cdh23	APN	10	60,465,543 (GRCm38)	missense	probably damaging	0.99
IGL02452:Cdh23	APN	10	60,317,942 (GRCm38)	missense	probably damaging	0.99
IGL02499:Cdh23	APN	10	60,385,179 (GRCm38)	missense	probably damaging	1.00
IGL02548:Cdh23	APN	10	60,650,122 (GRCm38)	missense	probably benign	0.37
IGL02593:Cdh23	APN	10	60,465,995 (GRCm38)	splice site	probably benign
IGL02626:Cdh23	APN	10	60,391,801 (GRCm38)	missense	probably damaging	1.00
IGL02951:Cdh23	APN	10	60,311,364 (GRCm38)	missense	probably damaging	1.00
IGL03145:Cdh23	APN	10	60,376,814 (GRCm38)	missense	probably damaging	0.99
dee_dee	UTSW	10	60,308,056 (GRCm38)	nonsense	probably null
hersey	UTSW	10	60,308,036 (GRCm38)	missense	probably damaging	1.00
ANU22:Cdh23	UTSW	10	60,312,624 (GRCm38)	missense	probably damaging	1.00
IGL02980:Cdh23	UTSW	10	60,314,620 (GRCm38)	missense	probably damaging	1.00
PIT4362001:Cdh23	UTSW	10	60,465,458 (GRCm38)	missense	probably benign	0.15
R0013:Cdh23	UTSW	10	60,413,173 (GRCm38)	missense	possibly damaging	0.90
R0045:Cdh23	UTSW	10	60,530,978 (GRCm38)	missense	probably damaging	1.00
R0045:Cdh23	UTSW	10	60,530,978 (GRCm38)	missense	probably damaging	1.00
R0082:Cdh23	UTSW	10	60,312,587 (GRCm38)	missense	probably damaging	1.00
R0124:Cdh23	UTSW	10	60,308,056 (GRCm38)	nonsense	probably null
R0172:Cdh23	UTSW	10	60,319,632 (GRCm38)	missense	probably damaging	1.00
R0195:Cdh23	UTSW	10	60,317,059 (GRCm38)	missense	probably damaging	0.99
R0365:Cdh23	UTSW	10	60,379,315 (GRCm38)	missense	probably damaging	0.99
R0437:Cdh23	UTSW	10	60,410,797 (GRCm38)	missense	probably damaging	1.00
R0486:Cdh23	UTSW	10	60,386,946 (GRCm38)	missense	probably damaging	1.00
R0494:Cdh23	UTSW	10	60,316,596 (GRCm38)	splice site	probably benign
R0545:Cdh23	UTSW	10	60,331,291 (GRCm38)	missense	probably benign	0.06
R0619:Cdh23	UTSW	10	60,433,777 (GRCm38)	missense	probably damaging	1.00
R0647:Cdh23	UTSW	10	60,323,374 (GRCm38)	nonsense	probably null
R0647:Cdh23	UTSW	10	60,307,902 (GRCm38)	missense	probably damaging	0.99
R0730:Cdh23	UTSW	10	60,323,714 (GRCm38)	missense	probably damaging	0.99
R0880:Cdh23	UTSW	10	60,406,421 (GRCm38)	missense	possibly damaging	0.51
R0942:Cdh23	UTSW	10	60,410,860 (GRCm38)	missense	possibly damaging	0.67
R0989:Cdh23	UTSW	10	60,534,510 (GRCm38)	missense	probably damaging	0.99
R1017:Cdh23	UTSW	10	60,331,793 (GRCm38)	missense	probably damaging	1.00
R1173:Cdh23	UTSW	10	60,312,392 (GRCm38)	splice site	probably benign
R1449:Cdh23	UTSW	10	60,376,951 (GRCm38)	missense	probably damaging	1.00
R1456:Cdh23	UTSW	10	60,487,120 (GRCm38)	missense	possibly damaging	0.84
R1519:Cdh23	UTSW	10	60,379,343 (GRCm38)	missense	possibly damaging	0.92
R1532:Cdh23	UTSW	10	60,314,331 (GRCm38)	missense	probably damaging	0.99
R1559:Cdh23	UTSW	10	60,419,699 (GRCm38)	splice site	probably benign
R1704:Cdh23	UTSW	10	60,314,611 (GRCm38)	missense	probably damaging	1.00
R1711:Cdh23	UTSW	10	60,523,536 (GRCm38)	missense	probably benign	0.07
R1760:Cdh23	UTSW	10	60,326,076 (GRCm38)	missense	probably damaging	1.00
R1782:Cdh23	UTSW	10	60,488,542 (GRCm38)	missense	probably damaging	1.00
R1791:Cdh23	UTSW	10	60,391,726 (GRCm38)	missense	possibly damaging	0.89
R1803:Cdh23	UTSW	10	60,331,281 (GRCm38)	missense	probably damaging	1.00
R1857:Cdh23	UTSW	10	60,323,297 (GRCm38)	missense	probably damaging	1.00
R1874:Cdh23	UTSW	10	60,436,818 (GRCm38)	missense	possibly damaging	0.52
R1914:Cdh23	UTSW	10	60,323,570 (GRCm38)	missense	probably damaging	0.99
R1958:Cdh23	UTSW	10	60,410,873 (GRCm38)	missense	probably benign	0.02
R1964:Cdh23	UTSW	10	60,385,222 (GRCm38)	missense	probably benign	0.31
R1966:Cdh23	UTSW	10	60,323,582 (GRCm38)	missense	probably damaging	1.00
R1981:Cdh23	UTSW	10	60,378,751 (GRCm38)	missense	probably damaging	1.00
R2010:Cdh23	UTSW	10	60,314,227 (GRCm38)	missense	probably damaging	0.99
R2036:Cdh23	UTSW	10	60,466,043 (GRCm38)	missense	possibly damaging	0.52
R2038:Cdh23	UTSW	10	60,312,587 (GRCm38)	missense	probably damaging	1.00
R2044:Cdh23	UTSW	10	60,596,730 (GRCm38)	missense	possibly damaging	0.72
R2111:Cdh23	UTSW	10	60,305,583 (GRCm38)	missense	probably damaging	0.99
R2112:Cdh23	UTSW	10	60,305,583 (GRCm38)	missense	probably damaging	0.99
R2211:Cdh23	UTSW	10	60,466,004 (GRCm38)	missense	possibly damaging	0.92
R2261:Cdh23	UTSW	10	60,317,128 (GRCm38)	missense	probably damaging	1.00
R2262:Cdh23	UTSW	10	60,317,128 (GRCm38)	missense	probably damaging	1.00
R2306:Cdh23	UTSW	10	60,323,445 (GRCm38)	missense	probably damaging	1.00
R2344:Cdh23	UTSW	10	60,316,724 (GRCm38)	missense	probably damaging	1.00
R2857:Cdh23	UTSW	10	60,382,653 (GRCm38)	critical splice donor site	probably null
R2858:Cdh23	UTSW	10	60,382,653 (GRCm38)	critical splice donor site	probably null
R2859:Cdh23	UTSW	10	60,382,653 (GRCm38)	critical splice donor site	probably null
R2876:Cdh23	UTSW	10	60,307,496 (GRCm38)	missense	probably damaging	1.00
R3034:Cdh23	UTSW	10	60,409,010 (GRCm38)	splice site	probably benign
R3424:Cdh23	UTSW	10	60,376,881 (GRCm38)	missense	possibly damaging	0.76
R3699:Cdh23	UTSW	10	60,327,370 (GRCm38)	critical splice donor site	probably null
R3700:Cdh23	UTSW	10	60,327,370 (GRCm38)	critical splice donor site	probably null
R3950:Cdh23	UTSW	10	60,657,326 (GRCm38)	missense	probably benign	0.04
R3951:Cdh23	UTSW	10	60,657,326 (GRCm38)	missense	probably benign	0.04
R3952:Cdh23	UTSW	10	60,657,326 (GRCm38)	missense	probably benign	0.04
R4108:Cdh23	UTSW	10	60,410,822 (GRCm38)	missense	possibly damaging	0.51
R4114:Cdh23	UTSW	10	60,421,040 (GRCm38)	splice site	probably null
R4273:Cdh23	UTSW	10	60,311,161 (GRCm38)	missense	possibly damaging	0.69
R4284:Cdh23	UTSW	10	60,303,493 (GRCm38)	missense	possibly damaging	0.91
R4334:Cdh23	UTSW	10	60,385,059 (GRCm38)	missense	probably damaging	0.99
R4474:Cdh23	UTSW	10	60,311,086 (GRCm38)	missense	probably damaging	1.00
R4532:Cdh23	UTSW	10	60,534,423 (GRCm38)	missense	probably benign	0.32
R4597:Cdh23	UTSW	10	60,409,044 (GRCm38)	missense	probably damaging	1.00
R4604:Cdh23	UTSW	10	60,337,666 (GRCm38)	missense	possibly damaging	0.93
R4793:Cdh23	UTSW	10	60,331,350 (GRCm38)	missense	probably damaging	1.00
R4816:Cdh23	UTSW	10	60,409,077 (GRCm38)	missense	possibly damaging	0.93
R4833:Cdh23	UTSW	10	60,385,038 (GRCm38)	missense	probably damaging	1.00
R4840:Cdh23	UTSW	10	60,419,777 (GRCm38)	missense	possibly damaging	0.53
R4857:Cdh23	UTSW	10	60,391,784 (GRCm38)	missense	probably damaging	1.00
R4869:Cdh23	UTSW	10	60,376,934 (GRCm38)	missense	probably damaging	1.00
R4894:Cdh23	UTSW	10	60,337,851 (GRCm38)	missense	probably benign	0.04
R4940:Cdh23	UTSW	10	60,307,935 (GRCm38)	missense	probably damaging	0.98
R5020:Cdh23	UTSW	10	60,308,032 (GRCm38)	missense	probably damaging	0.99
R5026:Cdh23	UTSW	10	60,304,848 (GRCm38)	missense	possibly damaging	0.88
R5081:Cdh23	UTSW	10	60,436,807 (GRCm38)	missense	possibly damaging	0.89
R5138:Cdh23	UTSW	10	60,312,282 (GRCm38)	missense	probably damaging	1.00
R5236:Cdh23	UTSW	10	60,312,572 (GRCm38)	missense	probably damaging	1.00
R5361:Cdh23	UTSW	10	60,657,265 (GRCm38)	critical splice donor site	probably null
R5384:Cdh23	UTSW	10	60,337,762 (GRCm38)	missense	probably damaging	0.99
R5500:Cdh23	UTSW	10	60,314,311 (GRCm38)	missense	probably damaging	1.00
R5512:Cdh23	UTSW	10	60,534,386 (GRCm38)	splice site	probably null
R5673:Cdh23	UTSW	10	60,307,857 (GRCm38)	missense	probably damaging	1.00
R5720:Cdh23	UTSW	10	60,393,023 (GRCm38)	missense	possibly damaging	0.71
R5726:Cdh23	UTSW	10	60,407,480 (GRCm38)	missense	probably damaging	0.98
R5732:Cdh23	UTSW	10	60,331,317 (GRCm38)	missense	possibly damaging	0.80
R5739:Cdh23	UTSW	10	60,305,609 (GRCm38)	missense	probably damaging	0.99
R5760:Cdh23	UTSW	10	60,406,392 (GRCm38)	missense	probably damaging	0.99
R5793:Cdh23	UTSW	10	60,306,128 (GRCm38)	missense	probably damaging	1.00
R5880:Cdh23	UTSW	10	60,384,934 (GRCm38)	missense	probably damaging	1.00
R5905:Cdh23	UTSW	10	60,534,535 (GRCm38)	missense	probably damaging	0.98
R5907:Cdh23	UTSW	10	60,428,379 (GRCm38)	missense	probably damaging	1.00
R5910:Cdh23	UTSW	10	60,377,821 (GRCm38)	missense	possibly damaging	0.81
R5932:Cdh23	UTSW	10	60,392,984 (GRCm38)	missense	probably damaging	1.00
R5996:Cdh23	UTSW	10	60,413,577 (GRCm38)	missense	possibly damaging	0.85
R6015:Cdh23	UTSW	10	60,307,982 (GRCm38)	missense	probably damaging	0.97
R6020:Cdh23	UTSW	10	60,331,326 (GRCm38)	missense	probably damaging	1.00
R6023:Cdh23	UTSW	10	60,465,542 (GRCm38)	missense	probably damaging	1.00
R6028:Cdh23	UTSW	10	60,534,535 (GRCm38)	missense	probably damaging	0.98
R6066:Cdh23	UTSW	10	60,433,758 (GRCm38)	missense	probably damaging	1.00
R6137:Cdh23	UTSW	10	60,434,512 (GRCm38)	missense	probably damaging	0.96
R6211:Cdh23	UTSW	10	60,410,821 (GRCm38)	missense	possibly damaging	0.90
R6298:Cdh23	UTSW	10	60,426,672 (GRCm38)	nonsense	probably null
R6302:Cdh23	UTSW	10	60,305,093 (GRCm38)	missense	possibly damaging	0.74
R6356:Cdh23	UTSW	10	60,438,847 (GRCm38)	missense	probably damaging	1.00
R6441:Cdh23	UTSW	10	60,308,036 (GRCm38)	missense	probably damaging	1.00
R6714:Cdh23	UTSW	10	60,331,830 (GRCm38)	missense	possibly damaging	0.62
R6760:Cdh23	UTSW	10	60,306,168 (GRCm38)	missense	probably damaging	1.00
R6807:Cdh23	UTSW	10	60,378,871 (GRCm38)	missense	possibly damaging	0.95
R6855:Cdh23	UTSW	10	60,306,122 (GRCm38)	missense	possibly damaging	0.66
R6937:Cdh23	UTSW	10	60,487,114 (GRCm38)	missense	probably damaging	1.00
R6942:Cdh23	UTSW	10	60,438,856 (GRCm38)	missense	possibly damaging	0.93
R6961:Cdh23	UTSW	10	60,650,114 (GRCm38)	missense	probably benign	0.00
R7009:Cdh23	UTSW	10	60,337,306 (GRCm38)	missense	probably damaging	0.99
R7010:Cdh23	UTSW	10	60,530,991 (GRCm38)	missense	probably benign	0.03
R7032:Cdh23	UTSW	10	60,331,788 (GRCm38)	missense	probably damaging	1.00
R7046:Cdh23	UTSW	10	60,378,751 (GRCm38)	missense	probably damaging	1.00
R7111:Cdh23	UTSW	10	60,387,044 (GRCm38)	missense	probably damaging	1.00
R7196:Cdh23	UTSW	10	60,307,980 (GRCm38)	missense	probably damaging	0.99
R7198:Cdh23	UTSW	10	60,312,599 (GRCm38)	missense	possibly damaging	0.91
R7223:Cdh23	UTSW	10	60,331,817 (GRCm38)	missense	probably damaging	1.00
R7290:Cdh23	UTSW	10	60,376,841 (GRCm38)	missense	probably benign
R7335:Cdh23	UTSW	10	60,305,116 (GRCm38)	missense	probably damaging	1.00
R7340:Cdh23	UTSW	10	60,530,996 (GRCm38)	missense	probably benign	0.19
R7350:Cdh23	UTSW	10	60,410,910 (GRCm38)	missense	probably damaging	1.00
R7366:Cdh23	UTSW	10	60,315,692 (GRCm38)	nonsense	probably null
R7374:Cdh23	UTSW	10	60,317,900 (GRCm38)	missense	probably damaging	0.99
R7455:Cdh23	UTSW	10	60,306,224 (GRCm38)	missense	possibly damaging	0.82
R7537:Cdh23	UTSW	10	60,384,945 (GRCm38)	missense	probably benign	0.17
R7573:Cdh23	UTSW	10	60,323,550 (GRCm38)	missense	probably benign	0.17
R7578:Cdh23	UTSW	10	60,407,407 (GRCm38)	missense	probably benign	0.14
R7646:Cdh23	UTSW	10	60,305,152 (GRCm38)	missense	possibly damaging	0.95
R7703:Cdh23	UTSW	10	60,337,264 (GRCm38)	missense	probably damaging	1.00
R7763:Cdh23	UTSW	10	60,312,577 (GRCm38)	missense	probably damaging	1.00
R7797:Cdh23	UTSW	10	60,385,194 (GRCm38)	missense	probably benign	0.07
R7867:Cdh23	UTSW	10	60,314,611 (GRCm38)	missense	probably damaging	1.00
R7878:Cdh23	UTSW	10	60,314,200 (GRCm38)	missense	possibly damaging	0.69
R7915:Cdh23	UTSW	10	60,307,889 (GRCm38)	missense	probably damaging	0.97
R7922:Cdh23	UTSW	10	60,382,706 (GRCm38)	missense	probably benign	0.31
R7963:Cdh23	UTSW	10	60,336,188 (GRCm38)	missense	probably damaging	1.00
R7997:Cdh23	UTSW	10	60,596,739 (GRCm38)	missense	possibly damaging	0.81
R8167:Cdh23	UTSW	10	60,314,383 (GRCm38)	missense	probably benign	0.12
R8167:Cdh23	UTSW	10	60,337,693 (GRCm38)	missense	probably damaging	0.96
R8258:Cdh23	UTSW	10	60,315,656 (GRCm38)	missense	probably damaging	0.99
R8259:Cdh23	UTSW	10	60,315,656 (GRCm38)	missense	probably damaging	0.99
R8317:Cdh23	UTSW	10	60,436,789 (GRCm38)	missense	probably damaging	1.00
R8317:Cdh23	UTSW	10	60,311,258 (GRCm38)	critical splice donor site	probably null
R8326:Cdh23	UTSW	10	60,438,812 (GRCm38)	missense	possibly damaging	0.55
R8333:Cdh23	UTSW	10	60,314,611 (GRCm38)	missense	probably damaging	1.00
R8348:Cdh23	UTSW	10	60,331,728 (GRCm38)	missense	probably benign	0.43
R8366:Cdh23	UTSW	10	60,325,020 (GRCm38)	missense	probably benign
R8504:Cdh23	UTSW	10	60,438,839 (GRCm38)	missense	probably benign	0.00
R8676:Cdh23	UTSW	10	60,410,910 (GRCm38)	missense	probably damaging	1.00
R8781:Cdh23	UTSW	10	60,331,788 (GRCm38)	missense	probably damaging	1.00
R8785:Cdh23	UTSW	10	60,311,335 (GRCm38)	missense	probably damaging	1.00
R8788:Cdh23	UTSW	10	60,488,593 (GRCm38)	missense	probably damaging	1.00
R8802:Cdh23	UTSW	10	60,409,098 (GRCm38)	missense	probably benign	0.04
R8837:Cdh23	UTSW	10	60,324,976 (GRCm38)	missense	probably benign	0.28
R8863:Cdh23	UTSW	10	60,376,834 (GRCm38)	nonsense	probably null
R8889:Cdh23	UTSW	10	60,307,505 (GRCm38)	missense	probably damaging	0.97
R8892:Cdh23	UTSW	10	60,307,505 (GRCm38)	missense	probably damaging	0.97
R8921:Cdh23	UTSW	10	60,305,129 (GRCm38)	missense	probably damaging	0.99
R8980:Cdh23	UTSW	10	60,337,846 (GRCm38)	missense	probably benign	0.06
R9000:Cdh23	UTSW	10	60,304,498 (GRCm38)	missense	possibly damaging	0.82
R9043:Cdh23	UTSW	10	60,315,699 (GRCm38)	missense	probably benign	0.00
R9046:Cdh23	UTSW	10	60,382,524 (GRCm38)	intron	probably benign
R9070:Cdh23	UTSW	10	60,337,760 (GRCm38)	missense	probably benign
R9075:Cdh23	UTSW	10	60,317,762 (GRCm38)	missense	probably damaging	1.00
R9132:Cdh23	UTSW	10	60,434,504 (GRCm38)	splice site	probably benign
R9155:Cdh23	UTSW	10	60,413,706 (GRCm38)	missense	probably damaging	0.99
R9171:Cdh23	UTSW	10	60,326,031 (GRCm38)	missense	probably benign	0.00
R9179:Cdh23	UTSW	10	60,317,885 (GRCm38)	missense	probably benign	0.06
R9186:Cdh23	UTSW	10	60,307,527 (GRCm38)	missense	possibly damaging	0.54
R9189:Cdh23	UTSW	10	60,307,527 (GRCm38)	missense	possibly damaging	0.54
R9207:Cdh23	UTSW	10	60,407,431 (GRCm38)	missense	probably damaging	1.00
R9240:Cdh23	UTSW	10	60,379,265 (GRCm38)	missense	probably benign	0.00
R9244:Cdh23	UTSW	10	60,413,663 (GRCm38)	missense	possibly damaging	0.93
R9284:Cdh23	UTSW	10	60,307,527 (GRCm38)	missense	possibly damaging	0.54
R9286:Cdh23	UTSW	10	60,307,527 (GRCm38)	missense	possibly damaging	0.54
R9287:Cdh23	UTSW	10	60,307,527 (GRCm38)	missense	possibly damaging	0.54
R9302:Cdh23	UTSW	10	60,307,527 (GRCm38)	missense	possibly damaging	0.54
R9352:Cdh23	UTSW	10	60,307,527 (GRCm38)	missense	possibly damaging	0.54
R9353:Cdh23	UTSW	10	60,307,527 (GRCm38)	missense	possibly damaging	0.54
R9423:Cdh23	UTSW	10	60,312,608 (GRCm38)	missense	probably damaging	1.00
R9513:Cdh23	UTSW	10	60,331,216 (GRCm38)	missense	probably damaging	0.99
R9577:Cdh23	UTSW	10	60,311,116 (GRCm38)	missense	probably damaging	1.00
R9598:Cdh23	UTSW	10	60,378,795 (GRCm38)	missense	probably benign	0.01
R9631:Cdh23	UTSW	10	60,407,389 (GRCm38)	missense	possibly damaging	0.49
R9652:Cdh23	UTSW	10	60,331,356 (GRCm38)	missense	probably damaging	1.00
R9725:Cdh23	UTSW	10	60,596,782 (GRCm38)	missense	probably benign	0.02
X0052:Cdh23	UTSW	10	60,385,134 (GRCm38)	missense	probably damaging	1.00
Z1088:Cdh23	UTSW	10	60,413,644 (GRCm38)	missense	probably benign	0.35
Z1176:Cdh23	UTSW	10	60,428,321 (GRCm38)	missense	probably benign
Z1176:Cdh23	UTSW	10	60,310,770 (GRCm38)	missense	probably damaging	1.00
Z1177:Cdh23	UTSW	10	60,434,614 (GRCm38)	critical splice acceptor site	probably null
Z1177:Cdh23	UTSW	10	60,323,555 (GRCm38)	missense	possibly damaging	0.80

Predicted Primers

PCR Primer
(F):5'- GGCTACTTGGCTTACATGCTG -3'
(R):5'- GGAAGAGACCCATGACTTAAGC -3'

Sequencing Primer
(F):5'- GGGTCTTAACTGGCATCCCTAG -3'
(R):5'- GATACTCAGTCTCCCATCT -3'

Posted On

2018-04-27