Mutagenetix > Incidental Mutation

Incidental Mutation 'R6377:Mki67'

515053

Institutional Source

Beutler Lab

Gene Symbol

Mki67

Ensembl Gene

ENSMUSG00000031004

Gene Name

antigen identified by monoclonal antibody Ki 67

Synonyms

D630048A14Rik, Ki-67, Ki67

MMRRC Submission

044527-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.872) question?

Stock #

R6377 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

135291513-135318090 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 135298050 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 2328 (V2328E)

Ref Sequence

ENSEMBL: ENSMUSP00000033310 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000033310]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000033310
AA Change: V2328E

PolyPhen 2 Score 0.670 (Sensitivity: 0.86; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000033310
Gene: ENSMUSG00000031004
AA Change: V2328E

Domain	Start	End	E-Value	Type
FHA	26	76	1.03e-11	SMART
Pfam:PP1_bind	462	519	2.8e-20	PFAM
low complexity region	535	545	N/A	INTRINSIC
low complexity region	869	882	N/A	INTRINSIC
Pfam:K167R	889	982	1.5e-9	PFAM
K167R	993	1102	2.01e-39	SMART
K167R	1107	1217	1.87e-57	SMART
K167R	1228	1337	1.33e-53	SMART
K167R	1348	1451	6.57e-44	SMART
K167R	1462	1570	9.09e-38	SMART
K167R	1580	1686	5.02e-40	SMART
K167R	1697	1807	9.6e-37	SMART
K167R	1818	1926	5.94e-51	SMART
K167R	1937	2047	1.6e-56	SMART
K167R	2058	2164	4.04e-53	SMART
K167R	2175	2285	1.52e-57	SMART
K167R	2296	2407	1.78e-40	SMART
K167R	2418	2527	1.71e-42	SMART
K167R	2538	2640	7.41e-20	SMART
K167R	2642	2750	1.06e-38	SMART
K167R	2761	2872	2.1e-42	SMART

Meta Mutation Damage Score

0.2527

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 97.8%

Validation Efficiency

94% (59/63)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
PHENOTYPE: Mice carrying a reporter allele show expression in actively dividing cells. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ankib1	A	G	5: 3,743,855 (GRCm39)	S812P	possibly damaging	Het
Atp2c2	A	G	8: 120,453,093 (GRCm39)	E159G	probably benign	Het
Atrn	A	G	2: 130,821,889 (GRCm39)	I861V	probably damaging	Het
Clip1	A	G	5: 123,741,717 (GRCm39)	V1053A	possibly damaging	Het
Cngb1	C	A	8: 95,975,608 (GRCm39)	G629C	probably damaging	Het
Cntn5	A	T	9: 9,743,657 (GRCm39)	F540Y	probably damaging	Het
Cpb1	A	C	3: 20,329,748 (GRCm39)		probably null	Het
Cyp4a14	A	G	4: 115,353,280 (GRCm39)	Y11H	probably benign	Het
Dact2	A	G	17: 14,419,450 (GRCm39)	S103P	probably damaging	Het
Dcstamp	A	T	15: 39,618,317 (GRCm39)	Y242F	probably benign	Het
Drd3	G	T	16: 43,641,670 (GRCm39)	G329*	probably null	Het
Duxf1	T	C	10: 58,059,494 (GRCm39)		probably benign	Het
Dysf	T	A	6: 83,985,945 (GRCm39)	S17T	probably benign	Het
Eno1	A	T	4: 150,333,009 (GRCm39)	K366N	possibly damaging	Het
Ffar2	C	T	7: 30,518,971 (GRCm39)	V190I	probably benign	Het
Fkbp15	T	C	4: 62,242,429 (GRCm39)	T508A	probably damaging	Het
Fndc1	A	G	17: 7,988,567 (GRCm39)	V1165A	unknown	Het
Foxj3	A	T	4: 119,430,945 (GRCm39)		probably null	Het
Gabarap	T	C	11: 69,882,630 (GRCm39)		probably null	Het
Gm19410	T	A	8: 36,270,736 (GRCm39)	L1221*	probably null	Het
Gpat2	G	C	2: 127,273,838 (GRCm39)	G294R	possibly damaging	Het
Igf1r	A	T	7: 67,850,998 (GRCm39)	Y931F	probably benign	Het
Il23r	A	T	6: 67,400,636 (GRCm39)	S565T	probably damaging	Het
Ipo8	A	T	6: 148,717,995 (GRCm39)	Y209*	probably null	Het
Jph2	C	T	2: 163,181,632 (GRCm39)	G511R	probably benign	Het
Khdrbs1	C	A	4: 129,635,890 (GRCm39)	D22Y	probably damaging	Het
Kif5b	A	T	18: 6,212,562 (GRCm39)	L754Q	probably damaging	Het
Ksr1	C	T	11: 78,927,320 (GRCm39)		probably null	Het
L3mbtl4	G	T	17: 69,084,918 (GRCm39)	V610F	probably benign	Het
Lars2	A	G	9: 123,283,825 (GRCm39)	T698A	probably benign	Het
Lonp1	A	G	17: 56,928,961 (GRCm39)	V267A	possibly damaging	Het
Loxhd1	T	A	18: 77,468,128 (GRCm39)	D925E	probably damaging	Het
Lsg1	A	G	16: 30,393,386 (GRCm39)	L187P	possibly damaging	Het
Mlh3	A	G	12: 85,315,271 (GRCm39)	I305T	probably damaging	Het
Mtbp	A	G	15: 55,421,016 (GRCm39)	M1V	probably null	Het
Myadml2	C	A	11: 120,538,538 (GRCm39)	C99F	probably benign	Het
Ncbp1	A	G	4: 46,150,703 (GRCm39)	Y185C	probably damaging	Het
Ndufaf7	A	T	17: 79,250,739 (GRCm39)	Q222L	probably null	Het
Nlrp4b	A	T	7: 10,449,339 (GRCm39)	Y147F	probably benign	Het
Or4c11c	C	T	2: 88,661,613 (GRCm39)	R51*	probably null	Het
Pet100	T	C	8: 3,672,370 (GRCm39)	V15A	probably benign	Het
Pot1a	A	G	6: 25,778,869 (GRCm39)	V75A	probably benign	Het
Ptprs	A	T	17: 56,725,935 (GRCm39)	M1043K	probably damaging	Het
Rnf31	A	G	14: 55,832,984 (GRCm39)	T413A	probably damaging	Het
Rsf1	ATGGCG	ATGGCGACGGTGGCG	7: 97,229,111 (GRCm39)		probably benign	Homo
Rsf1	CG	CGACGGCGGTG	7: 97,229,115 (GRCm39)		probably benign	Het
Rubcnl	A	T	14: 75,287,635 (GRCm39)		probably null	Het
Ryr3	A	T	2: 112,462,530 (GRCm39)	C4839S	probably damaging	Het
Scai	A	C	2: 38,992,340 (GRCm39)	D379E	probably benign	Het
Scd2	G	T	19: 44,288,198 (GRCm39)	G197*	probably null	Het
Sdr39u1	A	C	14: 56,135,166 (GRCm39)	I259S	probably benign	Het
Slco1a5	A	T	6: 142,187,906 (GRCm39)		probably null	Het
Sp1	A	T	15: 102,339,318 (GRCm39)	T733S	probably benign	Het
Tecta	A	G	9: 42,255,051 (GRCm39)	S1711P	probably damaging	Het
Tedc1	G	T	12: 113,124,975 (GRCm39)	W240L	probably damaging	Het
Trarg1	T	C	11: 76,571,355 (GRCm39)	S124P	probably damaging	Het
Trim50	A	G	5: 135,382,454 (GRCm39)	K102R	probably benign	Het
Utrn	A	G	10: 12,619,827 (GRCm39)	Y278H	probably damaging	Het
Vinac1	T	A	2: 128,878,731 (GRCm39)	D1065V	unknown	Het
Vmn2r109	A	C	17: 20,784,796 (GRCm39)		probably null	Het
Zbed5	T	C	5: 129,932,210 (GRCm39)	S720P	possibly damaging	Het
Zc3h3	A	G	15: 75,711,304 (GRCm39)	S386P	probably damaging	Het
Zscan4-ps1	C	T	7: 10,802,418 (GRCm39)		probably null	Het

Other mutations in Mki67

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00225:Mki67	APN	7	135,291,849 (GRCm39)	missense	probably benign	0.32
IGL00264:Mki67	APN	7	135,309,549 (GRCm39)	nonsense	probably null
IGL00328:Mki67	APN	7	135,298,424 (GRCm39)	missense	probably benign	0.03
IGL00570:Mki67	APN	7	135,309,830 (GRCm39)	missense	possibly damaging	0.88
IGL00584:Mki67	APN	7	135,297,424 (GRCm39)	missense	probably damaging	1.00
IGL00756:Mki67	APN	7	135,300,460 (GRCm39)	missense	possibly damaging	0.76
IGL01063:Mki67	APN	7	135,296,651 (GRCm39)	missense	possibly damaging	0.93
IGL01112:Mki67	APN	7	135,315,745 (GRCm39)	missense	probably damaging	1.00
IGL01360:Mki67	APN	7	135,307,505 (GRCm39)	missense	probably damaging	1.00
IGL01457:Mki67	APN	7	135,301,275 (GRCm39)	missense	probably benign	0.00
IGL01686:Mki67	APN	7	135,309,542 (GRCm39)	missense	probably benign	0.00
IGL01731:Mki67	APN	7	135,298,278 (GRCm39)	missense	probably benign	0.03
IGL01775:Mki67	APN	7	135,300,005 (GRCm39)	missense	possibly damaging	0.71
IGL01806:Mki67	APN	7	135,300,686 (GRCm39)	missense	probably damaging	0.98
IGL01860:Mki67	APN	7	135,300,686 (GRCm39)	missense	probably damaging	0.98
IGL01938:Mki67	APN	7	135,296,059 (GRCm39)	missense	probably benign	0.04
IGL02249:Mki67	APN	7	135,302,251 (GRCm39)	missense	possibly damaging	0.47
IGL02260:Mki67	APN	7	135,303,697 (GRCm39)	missense	probably benign	0.00
IGL02270:Mki67	APN	7	135,300,361 (GRCm39)	missense	probably damaging	1.00
IGL02406:Mki67	APN	7	135,300,522 (GRCm39)	missense	probably benign	0.00
IGL02499:Mki67	APN	7	135,296,056 (GRCm39)	missense	possibly damaging	0.94
IGL02655:Mki67	APN	7	135,315,748 (GRCm39)	missense	probably damaging	0.98
IGL02700:Mki67	APN	7	135,309,931 (GRCm39)	missense	probably benign	0.02
IGL03370:Mki67	APN	7	135,297,219 (GRCm39)	missense	probably benign	0.00
Advisement	UTSW	7	135,299,923 (GRCm39)	missense	probably damaging	1.00
chocotoff	UTSW	7	135,300,628 (GRCm39)	missense	possibly damaging	0.92
Godiva	UTSW	7	135,303,691 (GRCm39)	missense	probably benign	0.10
sees	UTSW	7	135,302,644 (GRCm39)	missense	possibly damaging	0.68
Whitman	UTSW	7	135,315,594 (GRCm39)	missense	probably damaging	1.00
BB003:Mki67	UTSW	7	135,298,869 (GRCm39)	missense	possibly damaging	0.91
BB013:Mki67	UTSW	7	135,298,869 (GRCm39)	missense	possibly damaging	0.91
PIT4468001:Mki67	UTSW	7	135,300,876 (GRCm39)	missense	probably benign	0.00
R0001:Mki67	UTSW	7	135,302,748 (GRCm39)	missense	probably damaging	0.99
R0001:Mki67	UTSW	7	135,300,901 (GRCm39)	missense	probably damaging	1.00
R0043:Mki67	UTSW	7	135,302,310 (GRCm39)	missense	probably benign	0.16
R0043:Mki67	UTSW	7	135,302,310 (GRCm39)	missense	probably benign	0.16
R0102:Mki67	UTSW	7	135,315,532 (GRCm39)	missense	probably benign	0.16
R0130:Mki67	UTSW	7	135,298,188 (GRCm39)	missense	probably damaging	1.00
R0149:Mki67	UTSW	7	135,300,153 (GRCm39)	missense	probably benign	0.00
R0356:Mki67	UTSW	7	135,306,135 (GRCm39)	missense	probably benign	0.34
R0482:Mki67	UTSW	7	135,301,158 (GRCm39)	missense	possibly damaging	0.60
R0508:Mki67	UTSW	7	135,302,075 (GRCm39)	missense	probably benign
R0532:Mki67	UTSW	7	135,299,893 (GRCm39)	nonsense	probably null
R0548:Mki67	UTSW	7	135,298,637 (GRCm39)	missense	possibly damaging	0.82
R0548:Mki67	UTSW	7	135,296,985 (GRCm39)	missense	probably damaging	1.00
R0557:Mki67	UTSW	7	135,300,990 (GRCm39)	missense	possibly damaging	0.48
R0627:Mki67	UTSW	7	135,309,987 (GRCm39)	missense	probably benign	0.31
R0631:Mki67	UTSW	7	135,306,117 (GRCm39)	missense	probably damaging	0.98
R0848:Mki67	UTSW	7	135,302,772 (GRCm39)	missense	probably benign	0.21
R1075:Mki67	UTSW	7	135,299,040 (GRCm39)	missense	probably benign	0.03
R1105:Mki67	UTSW	7	135,302,779 (GRCm39)	missense	probably benign	0.09
R1272:Mki67	UTSW	7	135,302,143 (GRCm39)	nonsense	probably null
R1331:Mki67	UTSW	7	135,300,005 (GRCm39)	missense	possibly damaging	0.71
R1486:Mki67	UTSW	7	135,301,449 (GRCm39)	missense	probably benign	0.00
R1510:Mki67	UTSW	7	135,297,900 (GRCm39)	missense	probably benign	0.26
R1573:Mki67	UTSW	7	135,296,845 (GRCm39)	missense	possibly damaging	0.93
R1586:Mki67	UTSW	7	135,315,701 (GRCm39)	nonsense	probably null
R1599:Mki67	UTSW	7	135,301,663 (GRCm39)	missense	probably benign	0.34
R1623:Mki67	UTSW	7	135,310,547 (GRCm39)	splice site	probably null
R1706:Mki67	UTSW	7	135,302,295 (GRCm39)	missense	probably benign	0.37
R1718:Mki67	UTSW	7	135,297,223 (GRCm39)	missense	probably damaging	1.00
R1785:Mki67	UTSW	7	135,305,970 (GRCm39)	critical splice acceptor site	probably null
R1816:Mki67	UTSW	7	135,309,116 (GRCm39)	missense	possibly damaging	0.68
R1862:Mki67	UTSW	7	135,301,090 (GRCm39)	missense	probably benign	0.09
R1929:Mki67	UTSW	7	135,299,794 (GRCm39)	missense	possibly damaging	0.46
R1957:Mki67	UTSW	7	135,300,128 (GRCm39)	missense	probably benign	0.01
R1971:Mki67	UTSW	7	135,315,688 (GRCm39)	critical splice donor site	probably null
R1998:Mki67	UTSW	7	135,307,499 (GRCm39)	missense	probably benign	0.00
R2004:Mki67	UTSW	7	135,300,238 (GRCm39)	nonsense	probably null
R2005:Mki67	UTSW	7	135,300,238 (GRCm39)	nonsense	probably null
R2006:Mki67	UTSW	7	135,300,238 (GRCm39)	nonsense	probably null
R2109:Mki67	UTSW	7	135,299,592 (GRCm39)	missense	probably damaging	1.00
R2130:Mki67	UTSW	7	135,305,970 (GRCm39)	critical splice acceptor site	probably null
R2131:Mki67	UTSW	7	135,305,970 (GRCm39)	critical splice acceptor site	probably null
R2133:Mki67	UTSW	7	135,305,970 (GRCm39)	critical splice acceptor site	probably null
R2140:Mki67	UTSW	7	135,297,321 (GRCm39)	missense	possibly damaging	0.94
R2141:Mki67	UTSW	7	135,297,321 (GRCm39)	missense	possibly damaging	0.94
R2142:Mki67	UTSW	7	135,297,321 (GRCm39)	missense	possibly damaging	0.94
R2284:Mki67	UTSW	7	135,301,674 (GRCm39)	missense	probably damaging	0.99
R2869:Mki67	UTSW	7	135,309,878 (GRCm39)	missense	probably benign	0.19
R2869:Mki67	UTSW	7	135,309,878 (GRCm39)	missense	probably benign	0.19
R2871:Mki67	UTSW	7	135,309,878 (GRCm39)	missense	probably benign	0.19
R2871:Mki67	UTSW	7	135,309,878 (GRCm39)	missense	probably benign	0.19
R2913:Mki67	UTSW	7	135,302,415 (GRCm39)	missense	possibly damaging	0.71
R3404:Mki67	UTSW	7	135,309,204 (GRCm39)	missense	probably benign	0.01
R3405:Mki67	UTSW	7	135,309,204 (GRCm39)	missense	probably benign	0.01
R3406:Mki67	UTSW	7	135,309,204 (GRCm39)	missense	probably benign	0.01
R3777:Mki67	UTSW	7	135,297,859 (GRCm39)	missense	probably benign	0.10
R3778:Mki67	UTSW	7	135,297,859 (GRCm39)	missense	probably benign	0.10
R3787:Mki67	UTSW	7	135,302,012 (GRCm39)	missense	possibly damaging	0.93
R3847:Mki67	UTSW	7	135,297,859 (GRCm39)	missense	probably benign	0.10
R3848:Mki67	UTSW	7	135,297,859 (GRCm39)	missense	probably benign	0.10
R3853:Mki67	UTSW	7	135,297,859 (GRCm39)	missense	probably benign	0.10
R3971:Mki67	UTSW	7	135,297,859 (GRCm39)	missense	probably benign	0.10
R3972:Mki67	UTSW	7	135,297,859 (GRCm39)	missense	probably benign	0.10
R4258:Mki67	UTSW	7	135,297,017 (GRCm39)	missense	possibly damaging	0.86
R4343:Mki67	UTSW	7	135,296,847 (GRCm39)	missense	probably benign	0.10
R4488:Mki67	UTSW	7	135,299,400 (GRCm39)	missense	probably benign	0.01
R4528:Mki67	UTSW	7	135,297,088 (GRCm39)	missense	probably damaging	1.00
R4713:Mki67	UTSW	7	135,297,198 (GRCm39)	missense	probably benign	0.35
R4867:Mki67	UTSW	7	135,301,585 (GRCm39)	missense	probably damaging	0.97
R4874:Mki67	UTSW	7	135,310,500 (GRCm39)	missense	probably damaging	0.97
R4897:Mki67	UTSW	7	135,298,474 (GRCm39)	missense	probably damaging	1.00
R5045:Mki67	UTSW	7	135,309,633 (GRCm39)	missense	possibly damaging	0.84
R5306:Mki67	UTSW	7	135,315,730 (GRCm39)	missense	probably damaging	1.00
R5309:Mki67	UTSW	7	135,302,559 (GRCm39)	missense	probably damaging	1.00
R5312:Mki67	UTSW	7	135,302,559 (GRCm39)	missense	probably damaging	1.00
R5379:Mki67	UTSW	7	135,299,190 (GRCm39)	missense	possibly damaging	0.95
R5506:Mki67	UTSW	7	135,301,710 (GRCm39)	missense	possibly damaging	0.60
R5513:Mki67	UTSW	7	135,309,479 (GRCm39)	missense	probably damaging	0.98
R5742:Mki67	UTSW	7	135,306,102 (GRCm39)	missense	probably benign	0.20
R5806:Mki67	UTSW	7	135,306,334 (GRCm39)	missense	probably damaging	1.00
R6008:Mki67	UTSW	7	135,299,158 (GRCm39)	missense	probably damaging	1.00
R6037:Mki67	UTSW	7	135,298,532 (GRCm39)	missense	possibly damaging	0.69
R6037:Mki67	UTSW	7	135,298,532 (GRCm39)	missense	possibly damaging	0.69
R6221:Mki67	UTSW	7	135,299,643 (GRCm39)	missense	probably benign	0.18
R6294:Mki67	UTSW	7	135,306,319 (GRCm39)	missense	probably benign	0.09
R6456:Mki67	UTSW	7	135,301,204 (GRCm39)	missense	possibly damaging	0.59
R6608:Mki67	UTSW	7	135,300,090 (GRCm39)	missense	probably benign	0.01
R6609:Mki67	UTSW	7	135,301,558 (GRCm39)	missense	possibly damaging	0.94
R6648:Mki67	UTSW	7	135,299,169 (GRCm39)	missense	probably damaging	1.00
R6901:Mki67	UTSW	7	135,310,489 (GRCm39)	splice site	probably null
R6978:Mki67	UTSW	7	135,303,691 (GRCm39)	missense	probably benign	0.10
R6985:Mki67	UTSW	7	135,315,594 (GRCm39)	missense	probably damaging	1.00
R7076:Mki67	UTSW	7	135,307,358 (GRCm39)	missense	probably damaging	0.98
R7217:Mki67	UTSW	7	135,305,911 (GRCm39)	missense	probably damaging	1.00
R7239:Mki67	UTSW	7	135,301,905 (GRCm39)	missense	possibly damaging	0.91
R7250:Mki67	UTSW	7	135,301,053 (GRCm39)	missense	possibly damaging	0.90
R7313:Mki67	UTSW	7	135,296,400 (GRCm39)	missense	probably benign	0.29
R7336:Mki67	UTSW	7	135,315,568 (GRCm39)	missense	probably benign	0.03
R7422:Mki67	UTSW	7	135,300,099 (GRCm39)	missense	probably damaging	1.00
R7451:Mki67	UTSW	7	135,301,080 (GRCm39)	missense	probably benign	0.01
R7502:Mki67	UTSW	7	135,302,512 (GRCm39)	missense	possibly damaging	0.53
R7513:Mki67	UTSW	7	135,294,952 (GRCm39)	missense	probably benign
R7578:Mki67	UTSW	7	135,302,644 (GRCm39)	missense	possibly damaging	0.68
R7619:Mki67	UTSW	7	135,301,106 (GRCm39)	missense	probably benign	0.01
R7646:Mki67	UTSW	7	135,298,498 (GRCm39)	missense	possibly damaging	0.63
R7659:Mki67	UTSW	7	135,299,155 (GRCm39)	missense	probably damaging	1.00
R7691:Mki67	UTSW	7	135,303,721 (GRCm39)	missense	not run
R7780:Mki67	UTSW	7	135,315,697 (GRCm39)	missense	probably benign	0.02
R7796:Mki67	UTSW	7	135,299,923 (GRCm39)	missense	probably damaging	1.00
R7904:Mki67	UTSW	7	135,294,816 (GRCm39)	missense	possibly damaging	0.90
R7911:Mki67	UTSW	7	135,306,333 (GRCm39)	missense	probably damaging	1.00
R7921:Mki67	UTSW	7	135,296,933 (GRCm39)	missense	probably benign	0.01
R7926:Mki67	UTSW	7	135,298,869 (GRCm39)	missense	possibly damaging	0.91
R7950:Mki67	UTSW	7	135,301,453 (GRCm39)	nonsense	probably null
R8130:Mki67	UTSW	7	135,299,293 (GRCm39)	missense	probably damaging	1.00
R8145:Mki67	UTSW	7	135,296,065 (GRCm39)	missense	probably benign	0.07
R8196:Mki67	UTSW	7	135,297,237 (GRCm39)	missense	probably damaging	1.00
R8220:Mki67	UTSW	7	135,299,850 (GRCm39)	missense	probably benign	0.03
R8299:Mki67	UTSW	7	135,306,349 (GRCm39)	missense	probably damaging	1.00
R8334:Mki67	UTSW	7	135,298,245 (GRCm39)	missense	probably damaging	0.98
R8350:Mki67	UTSW	7	135,300,200 (GRCm39)	missense	possibly damaging	0.82
R8358:Mki67	UTSW	7	135,301,855 (GRCm39)	missense	possibly damaging	0.46
R8529:Mki67	UTSW	7	135,315,688 (GRCm39)	critical splice donor site	probably null
R8698:Mki67	UTSW	7	135,296,937 (GRCm39)	missense	possibly damaging	0.87
R8700:Mki67	UTSW	7	135,307,436 (GRCm39)	missense
R8737:Mki67	UTSW	7	135,315,504 (GRCm39)	missense	probably damaging	1.00
R8914:Mki67	UTSW	7	135,299,595 (GRCm39)	missense
R8930:Mki67	UTSW	7	135,300,628 (GRCm39)	missense	possibly damaging	0.92
R8932:Mki67	UTSW	7	135,300,628 (GRCm39)	missense	possibly damaging	0.92
R8972:Mki67	UTSW	7	135,297,364 (GRCm39)	missense	possibly damaging	0.54
R8973:Mki67	UTSW	7	135,297,364 (GRCm39)	missense	possibly damaging	0.54
R8975:Mki67	UTSW	7	135,300,129 (GRCm39)	missense	probably benign	0.01
R8975:Mki67	UTSW	7	135,297,364 (GRCm39)	missense	possibly damaging	0.54
R9071:Mki67	UTSW	7	135,301,205 (GRCm39)	missense	probably benign	0.00
R9241:Mki67	UTSW	7	135,297,653 (GRCm39)	missense	possibly damaging	0.93
R9387:Mki67	UTSW	7	135,302,378 (GRCm39)	missense	probably damaging	0.99
R9524:Mki67	UTSW	7	135,305,913 (GRCm39)	missense	probably damaging	1.00
R9565:Mki67	UTSW	7	135,309,233 (GRCm39)	frame shift	probably null
R9782:Mki67	UTSW	7	135,306,066 (GRCm39)	critical splice donor site	probably null
X0020:Mki67	UTSW	7	135,315,730 (GRCm39)	missense	probably damaging	0.96
X0065:Mki67	UTSW	7	135,315,573 (GRCm39)	missense	possibly damaging	0.95

Predicted Primers

PCR Primer
(F):5'- TTGGAAGACCTCCTGAACAC -3'
(R):5'- AGAAGACCTGGATGGCTTCC -3'

Sequencing Primer
(F):5'- GGACTGGGACCTTTTCTTACATGAC -3'
(R):5'- GGATGGCTTCCAAGAACTCTTC -3'

Posted On

2018-05-04