Mutagenetix > Incidental Mutation

Incidental Mutation 'R6377:Dact2'

515079

Institutional Source

Beutler Lab

Gene Symbol

Dact2

Ensembl Gene

ENSMUSG00000048826

Gene Name

dishevelled-binding antagonist of beta-catenin 2

Synonyms

dapper2, Dpr2, Frd2, 2900084M21Rik

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6377 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

14195231-14203831 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 14199188 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 103 (S103P)

Ref Sequence

ENSEMBL: ENSMUSP00000051638 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053218]

AlphaFold

Q7TN08

Predicted Effect

probably damaging
Transcript: ENSMUST00000053218
AA Change: S103P

PolyPhen 2 Score 0.970 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000051638
Gene: ENSMUSG00000048826
AA Change: S103P

Domain	Start	End	E-Value	Type
Pfam:Dapper	19	757	1.8e-231	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 97.8%

Validation Efficiency

94% (59/63)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele develop normally but display enhanced keratinocyte migration and accelerated re-epithelialization during cutaneous wound healing. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ankib1	A	G	5: 3,693,855	S812P	possibly damaging	Het
Atp2c2	A	G	8: 119,726,354	E159G	probably benign	Het
Atrn	A	G	2: 130,979,969	I861V	probably damaging	Het
AW822073	T	C	10: 58,223,672		probably benign	Het
Clip1	A	G	5: 123,603,654	V1053A	possibly damaging	Het
Cngb1	C	A	8: 95,248,980	G629C	probably damaging	Het
Cntn5	A	T	9: 9,743,652	F540Y	probably damaging	Het
Cpb1	A	C	3: 20,275,584		probably null	Het
Cyp4a14	A	G	4: 115,496,083	Y11H	probably benign	Het
Dcstamp	A	T	15: 39,754,921	Y242F	probably benign	Het
Drd3	G	T	16: 43,821,307	G329*	probably null	Het
Dysf	T	A	6: 84,008,963	S17T	probably benign	Het
Eno1	A	T	4: 150,248,552	K366N	possibly damaging	Het
Ffar2	C	T	7: 30,819,546	V190I	probably benign	Het
Fkbp15	T	C	4: 62,324,192	T508A	probably damaging	Het
Fndc1	A	G	17: 7,769,735	V1165A	unknown	Het
Foxj3	A	T	4: 119,573,748		probably null	Het
Gabarap	T	C	11: 69,991,804		probably null	Het
Gm14025	T	A	2: 129,036,811	D1065V	unknown	Het
Gm19410	T	A	8: 35,803,582	L1221*	probably null	Het
Gpat2	G	C	2: 127,431,918	G294R	possibly damaging	Het
Igf1r	A	T	7: 68,201,250	Y931F	probably benign	Het
Il23r	A	T	6: 67,423,652	S565T	probably damaging	Het
Ipo8	A	T	6: 148,816,497	Y209*	probably null	Het
Jph2	C	T	2: 163,339,712	G511R	probably benign	Het
Khdrbs1	C	A	4: 129,742,097	D22Y	probably damaging	Het
Kif5b	A	T	18: 6,212,562	L754Q	probably damaging	Het
Ksr1	C	T	11: 79,036,494		probably null	Het
L3mbtl4	G	T	17: 68,777,923	V610F	probably benign	Het
Lars2	A	G	9: 123,454,760	T698A	probably benign	Het
Lonp1	A	G	17: 56,621,961	V267A	possibly damaging	Het
Loxhd1	T	A	18: 77,380,432	D925E	probably damaging	Het
Lsg1	A	G	16: 30,574,568	L187P	possibly damaging	Het
Mki67	A	T	7: 135,696,321	V2328E	possibly damaging	Het
Mlh3	A	G	12: 85,268,497	I305T	probably damaging	Het
Mtbp	A	G	15: 55,557,620	M1V	probably null	Het
Myadml2	C	A	11: 120,647,712	C99F	probably benign	Het
Ncbp1	A	G	4: 46,150,703	Y185C	probably damaging	Het
Ndufaf7	A	T	17: 78,943,310	Q222L	probably null	Het
Nlrp4b	A	T	7: 10,715,412	Y147F	probably benign	Het
Olfr1205	C	T	2: 88,831,269	R51*	probably null	Het
Pet100	T	C	8: 3,622,370	V15A	probably benign	Het
Pot1a	A	G	6: 25,778,870	V75A	probably benign	Het
Ptprs	A	T	17: 56,418,935	M1043K	probably damaging	Het
Rnf31	A	G	14: 55,595,527	T413A	probably damaging	Het
Rsf1	ATGGCG	ATGGCGACGGTGGCG	7: 97,579,904		probably benign	Homo
Rsf1	CG	CGACGGCGGTG	7: 97,579,908		probably benign	Het
Rubcnl	A	T	14: 75,050,195		probably null	Het
Ryr3	A	T	2: 112,632,185	C4839S	probably damaging	Het
Scai	A	C	2: 39,102,328	D379E	probably benign	Het
Scd2	G	T	19: 44,299,759	G197*	probably null	Het
Sdr39u1	A	C	14: 55,897,709	I259S	probably benign	Het
Slco1a5	A	T	6: 142,242,180		probably null	Het
Sp1	A	T	15: 102,430,883	T733S	probably benign	Het
Tecta	A	G	9: 42,343,755	S1711P	probably damaging	Het
Tedc1	G	T	12: 113,161,355	W240L	probably damaging	Het
Trim50	A	G	5: 135,353,600	K102R	probably benign	Het
Tusc5	T	C	11: 76,680,529	S124P	probably damaging	Het
Utrn	A	G	10: 12,744,083	Y278H	probably damaging	Het
Vmn2r109	A	C	17: 20,564,534		probably null	Het
Zbed5	T	C	5: 129,903,369	S720P	possibly damaging	Het
Zc3h3	A	G	15: 75,839,455	S386P	probably damaging	Het
Zscan4-ps1	C	T	7: 11,068,491		probably null	Het

Other mutations in Dact2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01867:Dact2	APN	17	14195670	missense	probably damaging	0.98
R0597:Dact2	UTSW	17	14197041	missense	probably benign
R1657:Dact2	UTSW	17	14197990	missense	probably benign	0.03
R1717:Dact2	UTSW	17	14197913	missense	probably benign	0.11
R1734:Dact2	UTSW	17	14196639	missense	probably benign	0.00
R1883:Dact2	UTSW	17	14197823	missense	possibly damaging	0.81
R2238:Dact2	UTSW	17	14197050	missense	probably damaging	0.99
R2878:Dact2	UTSW	17	14195914	missense	probably damaging	1.00
R4290:Dact2	UTSW	17	14196571	missense	probably benign	0.00
R4291:Dact2	UTSW	17	14196571	missense	probably benign	0.00
R4902:Dact2	UTSW	17	14196729	missense	possibly damaging	0.91
R5029:Dact2	UTSW	17	14195852	missense	probably benign	0.00
R5084:Dact2	UTSW	17	14197952	missense	possibly damaging	0.94
R5436:Dact2	UTSW	17	14195748	missense	probably damaging	1.00
R5837:Dact2	UTSW	17	14196253	missense	probably damaging	1.00
R5847:Dact2	UTSW	17	14199188	missense	probably damaging	0.97
R6048:Dact2	UTSW	17	14197305	missense	probably damaging	1.00
R7013:Dact2	UTSW	17	14203534	missense	probably benign
R7268:Dact2	UTSW	17	14196535	missense	probably benign	0.01
R7425:Dact2	UTSW	17	14196331	missense	probably damaging	1.00
R8725:Dact2	UTSW	17	14196884	nonsense	probably null
R8727:Dact2	UTSW	17	14196884	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ACACAGGCTGAAGCTGTCAC -3'
(R):5'- CAGCAGATGACAGTTAGGTGTG -3'

Sequencing Primer
(F):5'- ACAGGCTGAAGCTGTCACTATTC -3'
(R):5'- GGTGTTTGTGATAGCTCTCTTTCACC -3'

Posted On

2018-05-04