Mutagenetix > Incidental Mutation

Incidental Mutation 'R6383:Zfp882'

515445

Institutional Source

Beutler Lab

Gene Symbol

Zfp882

Ensembl Gene

ENSMUSG00000089857

Gene Name

zinc finger protein 882

Synonyms

ENSMUSG00000052439

MMRRC Submission

044532-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.092) question?

Stock #

R6383 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

71908608-71916354 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 71914640 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Arginine at position 437 (H437R)

Ref Sequence

ENSEMBL: ENSMUSP00000105629 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000110002] [ENSMUST00000125802] [ENSMUST00000126607] [ENSMUST00000131544]

AlphaFold

E9Q4R4

Predicted Effect

probably damaging
Transcript: ENSMUST00000110002
AA Change: H437R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000105629
Gene: ENSMUSG00000089857
AA Change: H437R

Domain	Start	End	E-Value	Type
KRAB	4	61	8.58e-14	SMART
ZnF_C2H2	84	106	1.47e-3	SMART
ZnF_C2H2	168	190	8.47e-4	SMART
ZnF_C2H2	196	218	2.27e-4	SMART
ZnF_C2H2	224	246	6.31e1	SMART
ZnF_C2H2	251	273	1.16e-1	SMART
ZnF_C2H2	279	301	1.25e-1	SMART
ZnF_C2H2	307	329	5.42e-2	SMART
ZnF_C2H2	335	357	1.47e-3	SMART
ZnF_C2H2	363	385	7.26e-3	SMART
ZnF_C2H2	391	413	1.26e-2	SMART
ZnF_C2H2	419	441	3.29e1	SMART
ZnF_C2H2	447	469	2.67e-1	SMART
ZnF_C2H2	475	497	1.04e-3	SMART
ZnF_C2H2	503	525	4.11e-2	SMART
ZnF_C2H2	531	553	4.87e-4	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000118290

Predicted Effect

probably benign
Transcript: ENSMUST00000125802

SMART Domains

Protein: ENSMUSP00000121316
Gene: ENSMUSG00000089857

Domain	Start	End	E-Value	Type
KRAB	12	69	8.58e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000126607

SMART Domains

Protein: ENSMUSP00000119978
Gene: ENSMUSG00000089857

Domain	Start	End	E-Value	Type
KRAB	44	101	8.58e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000131544

SMART Domains

Protein: ENSMUSP00000120213
Gene: ENSMUSG00000066880

Domain	Start	End	E-Value	Type
KRAB	4	56	1.08e-10	SMART
ZnF_C2H2	167	189	8.47e-4	SMART
ZnF_C2H2	195	217	8.34e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000170898

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 97.9%

Validation Efficiency

97% (73/75)

Allele List at MGI

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca12	A	T	1: 71,247,184 (GRCm38)	M2497K	probably benign	Het
Abtb2	C	T	2: 103,567,376 (GRCm38)	T217I	probably damaging	Het
Adam29	T	A	8: 55,871,508 (GRCm38)	N637I	probably damaging	Het
Adgb	T	A	10: 10,450,028 (GRCm38)	E59V	probably damaging	Het
Adh7	C	T	3: 138,228,017 (GRCm38)	R312C	probably benign	Het
Adprh	A	G	16: 38,447,452 (GRCm38)	I157T	probably damaging	Het
Ap2b1	T	C	11: 83,346,825 (GRCm38)	S572P	probably damaging	Het
Asic1	T	C	15: 99,698,880 (GRCm38)	L519P	probably damaging	Het
Atp2a2	G	C	5: 122,501,649 (GRCm38)	L13V	probably benign	Het
Bst2	A	T	8: 71,537,288 (GRCm38)	I47N	possibly damaging	Het
Cacng6	G	A	7: 3,424,993 (GRCm38)		probably null	Het
Cenpe	A	G	3: 135,251,528 (GRCm38)	E1849G	probably damaging	Het
Cep295	T	A	9: 15,332,754 (GRCm38)	T213S	probably damaging	Het
Chia1	A	G	3: 106,131,811 (GRCm38)	T406A	probably benign	Het
Chmp4c	G	T	3: 10,367,217 (GRCm38)	K62N	probably damaging	Het
Cldn15	A	G	5: 136,968,125 (GRCm38)	T7A	probably benign	Het
Cmpk2	T	A	12: 26,478,020 (GRCm38)	M412K	probably benign	Het
Cnnm1	T	C	19: 43,465,266 (GRCm38)		probably null	Het
Cubn	A	C	2: 13,427,835 (GRCm38)		probably null	Het
Dopey2	T	C	16: 93,782,248 (GRCm38)	V1668A	possibly damaging	Het
Erg28	T	A	12: 85,816,429 (GRCm38)	Y77F	probably damaging	Het
F830045P16Rik	C	T	2: 129,536,438 (GRCm38)	A9T	probably benign	Het
Gli3	A	G	13: 15,723,555 (GRCm38)	D740G	probably damaging	Het
Gm13128	A	T	4: 144,333,147 (GRCm38)	*476L	probably null	Het
Gm14085	T	C	2: 122,524,807 (GRCm38)	I555T	probably benign	Het
Gpat2	G	C	2: 127,431,918 (GRCm38)	G294R	possibly damaging	Het
Gpat3	A	T	5: 100,893,144 (GRCm38)	M357L	probably benign	Het
Gpr179	A	G	11: 97,337,147 (GRCm38)	V1394A	possibly damaging	Het
Grn	T	A	11: 102,436,795 (GRCm38)		probably benign	Het
H2-Q6	G	A	17: 35,428,383 (GRCm38)		probably null	Het
Igsf3	A	G	3: 101,435,648 (GRCm38)	T514A	probably benign	Het
Il1r1	A	G	1: 40,313,335 (GRCm38)	D558G	possibly damaging	Het
Irx4	T	C	13: 73,267,713 (GRCm38)	M207T	possibly damaging	Het
Kap	T	C	6: 133,851,957 (GRCm38)	I54V	probably benign	Het
Kdm2b	G	A	5: 122,934,778 (GRCm38)	R340C	probably damaging	Het
Lipo3	T	A	19: 33,556,431 (GRCm38)	M334L	probably benign	Het
Lmbrd1	C	T	1: 24,706,034 (GRCm38)	L152F	probably damaging	Het
Ltbp1	G	A	17: 75,359,457 (GRCm38)	V1382I	probably damaging	Het
Map3k4	C	T	17: 12,249,583 (GRCm38)	D1008N	possibly damaging	Het
Mcf2l	T	C	8: 12,879,912 (GRCm38)		probably benign	Het
Mecom	T	G	3: 29,997,726 (GRCm38)	D180A	probably damaging	Het
Meis1	T	C	11: 18,941,741 (GRCm38)	D269G	probably benign	Het
Myh7	A	C	14: 54,988,894 (GRCm38)	S430A	probably benign	Het
Myo1h	A	G	5: 114,336,264 (GRCm38)	I439V	probably damaging	Het
Nat1	T	C	8: 67,491,482 (GRCm38)	V170A	possibly damaging	Het
Nlrp12	A	G	7: 3,234,043 (GRCm38)	L742P	probably damaging	Het
Nlrp4c	A	G	7: 6,066,053 (GRCm38)	T318A	probably benign	Het
Olfr1297	T	A	2: 111,621,186 (GRCm38)	N296I	probably benign	Het
Olfr1425	T	A	19: 12,074,363 (GRCm38)	I90F	probably damaging	Het
Olfr632	A	G	7: 103,937,823 (GRCm38)	I148V	probably benign	Het
Olfr952	G	T	9: 39,426,234 (GRCm38)	T279N	probably damaging	Het
Olfr955	A	T	9: 39,470,630 (GRCm38)	L32Q	probably damaging	Het
Otop3	A	G	11: 115,345,072 (GRCm38)	E529G	probably damaging	Het
Parp6	T	C	9: 59,623,939 (GRCm38)	Y35H	probably damaging	Het
Pcdhb4	A	C	18: 37,308,021 (GRCm38)	D128A	probably damaging	Het
Phldb2	C	T	16: 45,748,750 (GRCm38)	D1249N	probably damaging	Het
Ptpn12	T	A	5: 20,987,468 (GRCm38)	K765*	probably null	Het
Ptprb	T	A	10: 116,347,007 (GRCm38)	Y1529*	probably null	Het
Ptprc	A	T	1: 138,078,451 (GRCm38)	Y798N	possibly damaging	Het
Sdk2	G	A	11: 113,832,265 (GRCm38)	T1300I	probably damaging	Het
Sptbn2	G	A	19: 4,732,496 (GRCm38)	V487I	possibly damaging	Het
Sptbn5	A	G	2: 120,046,269 (GRCm38)		probably benign	Het
Srpk1	A	G	17: 28,590,062 (GRCm38)	S648P	probably damaging	Het
Stard9	T	C	2: 120,666,407 (GRCm38)		probably null	Het
Tmem135	A	T	7: 89,144,670 (GRCm38)	I388N	probably damaging	Het
Top3a	A	T	11: 60,749,459 (GRCm38)	I446N	probably benign	Het
Trpv1	T	C	11: 73,246,036 (GRCm38)	S482P	probably damaging	Het
Ugt3a1	C	T	15: 9,306,455 (GRCm38)	A230V	probably benign	Het
Vmn2r15	A	C	5: 109,293,226 (GRCm38)	Y255*	probably null	Het
Vmn2r60	A	G	7: 42,116,471 (GRCm38)	M1V	probably null	Het
Vmn2r87	A	G	10: 130,479,000 (GRCm38)	V239A	probably damaging	Het
Vwce	T	A	19: 10,659,592 (GRCm38)	C679*	probably null	Het
Zfp385b	C	T	2: 77,415,841 (GRCm38)	A281T	probably benign	Het
Zfp398	T	G	6: 47,866,595 (GRCm38)	L395W	probably damaging	Het
Zfp442	C	T	2: 150,451,401 (GRCm38)		probably null	Het
Zfp606	A	G	7: 12,492,944 (GRCm38)	S331G	probably benign	Het

Other mutations in Zfp882

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00676:Zfp882	APN	8	71,913,827 (GRCm38)	missense	probably benign
R0244:Zfp882	UTSW	8	71,913,523 (GRCm38)	missense	possibly damaging	0.79
R0270:Zfp882	UTSW	8	71,914,615 (GRCm38)	missense	probably benign	0.05
R0636:Zfp882	UTSW	8	71,914,337 (GRCm38)	missense	probably benign	0.01
R0840:Zfp882	UTSW	8	71,914,686 (GRCm38)	nonsense	probably null
R1299:Zfp882	UTSW	8	71,913,473 (GRCm38)	missense	probably damaging	1.00
R4439:Zfp882	UTSW	8	71,913,609 (GRCm38)	missense	probably damaging	0.97
R4829:Zfp882	UTSW	8	71,914,389 (GRCm38)	missense	probably damaging	1.00
R5028:Zfp882	UTSW	8	71,914,654 (GRCm38)	missense	possibly damaging	0.70
R5296:Zfp882	UTSW	8	71,914,360 (GRCm38)	missense	probably damaging	1.00
R5882:Zfp882	UTSW	8	71,913,459 (GRCm38)	critical splice acceptor site	probably null
R5974:Zfp882	UTSW	8	71,913,155 (GRCm38)	missense	probably damaging	1.00
R6052:Zfp882	UTSW	8	71,914,505 (GRCm38)	missense	probably benign	0.01
R6888:Zfp882	UTSW	8	71,914,286 (GRCm38)	missense	probably benign	0.01
R6987:Zfp882	UTSW	8	71,914,673 (GRCm38)	missense	probably benign	0.01
R7045:Zfp882	UTSW	8	71,913,249 (GRCm38)	critical splice donor site	probably null
R7780:Zfp882	UTSW	8	71,914,229 (GRCm38)	missense	possibly damaging	0.89
R7793:Zfp882	UTSW	8	71,913,141 (GRCm38)	missense	probably damaging	1.00
R8386:Zfp882	UTSW	8	71,914,118 (GRCm38)	missense	probably benign	0.00
R9452:Zfp882	UTSW	8	71,914,987 (GRCm38)	missense	probably damaging	1.00
R9694:Zfp882	UTSW	8	71,914,071 (GRCm38)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TTTGAGGAAGGTTGAAAGTCATGA -3'
(R):5'- GGCTTACACACAGTGCACTT -3'

Sequencing Primer
(F):5'- TGGGAAAGCCTTCATGCATC -3'
(R):5'- GGAACCATGAGTACTGTAGGATTTCC -3'

Posted On

2018-05-04