Incidental Mutation 'R6386:Prl3d2'
ID515613
Institutional Source Beutler Lab
Gene Symbol Prl3d2
Ensembl Gene ENSMUSG00000062737
Gene Nameprolactin family 3, subfamily d, member 1
SynonymsPlib, PL-Ib
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.064) question?
Stock #R6386 (G1)
Quality Score225.009
Status Not validated
Chromosome13
Chromosomal Location27121698-27127555 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 27127303 bp
ZygosityHeterozygous
Amino Acid Change Aspartic acid to Glycine at position 186 (D186G)
Ref Sequence ENSEMBL: ENSMUSP00000079579 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000080755] [ENSMUST00000164964]
Predicted Effect probably damaging
Transcript: ENSMUST00000080755
AA Change: D186G

PolyPhen 2 Score 0.970 (Sensitivity: 0.77; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000079579
Gene: ENSMUSG00000062737
AA Change: D186G

DomainStartEndE-ValueType
Pfam:Hormone_1 17 224 4.5e-72 PFAM
Predicted Effect possibly damaging
Transcript: ENSMUST00000164964
AA Change: D187G

PolyPhen 2 Score 0.777 (Sensitivity: 0.85; Specificity: 0.92)
SMART Domains Protein: ENSMUSP00000130458
Gene: ENSMUSG00000062737
AA Change: D187G

DomainStartEndE-ValueType
Pfam:Hormone_1 16 223 3e-69 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.1%
  • 20x: 96.6%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 36 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ak6 TGACGA TGA 13: 100,655,803 probably benign Het
Arap2 A T 5: 62,604,522 N1620K possibly damaging Het
Atf6b T C 17: 34,651,851 S396P probably damaging Het
Cc2d1a A G 8: 84,138,537 M469T probably damaging Het
Ceacam3 A G 7: 17,158,219 N296D probably benign Het
Cep57l1 A T 10: 41,743,132 S80T probably damaging Het
Clip4 C A 17: 71,834,194 Y514* probably null Het
Cop1 T C 1: 159,289,031 I125T probably damaging Het
Cstf1 T C 2: 172,377,896 V309A probably damaging Het
Cyp4a29 T G 4: 115,247,075 probably null Het
F830045P16Rik G A 2: 129,472,818 H180Y probably damaging Het
Foxp4 T C 17: 47,878,462 K237E unknown Het
Fstl5 A T 3: 76,322,066 H58L probably benign Het
Gje1 A G 10: 14,716,621 F139S probably damaging Het
Gpatch1 T C 7: 35,291,840 D593G probably damaging Het
Inpp5d T A 1: 87,699,675 L566Q probably damaging Het
Mtch2 A G 2: 90,849,395 T38A probably benign Het
Mvb12b A T 2: 33,827,742 I129N probably damaging Het
Npffr2 T C 5: 89,582,697 V162A probably benign Het
Olfr1424 A T 19: 12,059,556 N65K probably damaging Het
Olfr485 A T 7: 108,159,202 Y224N probably damaging Het
Pkhd1 G A 1: 20,551,020 R805C probably damaging Het
Ppp4r4 T C 12: 103,593,105 L406P probably damaging Het
Rfc5 T C 5: 117,385,398 T112A probably benign Het
Rnf148 A G 6: 23,654,484 L171P probably damaging Het
Rps24 G A 14: 24,492,048 G71S possibly damaging Het
Slco2a1 G A 9: 103,076,988 V453I probably benign Het
Spidr T C 16: 15,968,560 K440E probably benign Het
Syndig1 C A 2: 149,899,576 N27K probably damaging Het
Tmem62 C T 2: 120,999,114 T316I probably benign Het
Tpgs2 A T 18: 25,139,024 I258N possibly damaging Het
Vmn2r78 A T 7: 86,922,337 R452* probably null Het
Wasf3 A T 5: 146,453,417 I124F possibly damaging Het
Wbp11 T C 6: 136,820,525 T299A probably benign Het
Wdr25 T C 12: 109,025,065 S41P probably damaging Het
Zfp874b T C 13: 67,474,843 D116G possibly damaging Het
Other mutations in Prl3d2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00899:Prl3d2 APN 13 27122349 missense probably damaging 1.00
IGL01362:Prl3d2 APN 13 27122455 nonsense probably null
IGL02647:Prl3d2 APN 13 27126016 missense probably benign 0.06
IGL02751:Prl3d2 APN 13 27126031 critical splice donor site probably null
IGL02992:Prl3d2 APN 13 27127283 missense probably benign 0.00
R1116:Prl3d2 UTSW 13 27126002 missense probably damaging 1.00
R1500:Prl3d2 UTSW 13 27121706 unclassified probably benign
R4713:Prl3d2 UTSW 13 27122396 missense probably benign
R5193:Prl3d2 UTSW 13 27122329 missense possibly damaging 0.87
R7830:Prl3d2 UTSW 13 27126017 missense probably benign 0.00
R7999:Prl3d2 UTSW 13 27123966 missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- CCCCAAATTGAGTGCTGACATC -3'
(R):5'- GCATTACAAGTCTGGTTCAACAAAG -3'

Sequencing Primer
(F):5'- GAGTGCTGACATCCAAATCTTGTG -3'
(R):5'- GTCTGGTTCAACAAAGACCATGTG -3'
Posted On2018-05-04