Mutagenetix > Incidental Mutation

Incidental Mutation 'R6391:Vmn2r26'

515762

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r26

Ensembl Gene

ENSMUSG00000096630

Gene Name

vomeronasal 2, receptor 26

Synonyms

V2r1b

MMRRC Submission

044540-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.088) question?

Stock #

R6391 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

124024758-124062035 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 124061389 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 641 (L641P)

Ref Sequence

ENSEMBL: ENSMUSP00000032238 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032238]

AlphaFold

Q6TAC4

Predicted Effect

probably damaging
Transcript: ENSMUST00000032238
AA Change: L641P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000032238
Gene: ENSMUSG00000096630
AA Change: L641P

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	82	471	1.5e-31	PFAM
Pfam:NCD3G	519	572	4.6e-25	PFAM
Pfam:7tm_3	603	840	1.5e-55	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000158682

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 98.2%

Validation Efficiency

98% (40/41)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit abnormal vomeronasal sensory neuron physiology and avnosmia. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Bcl7b	G	T	5: 135,180,025 (GRCm38)	S114I	probably damaging	Het
Cct8	A	T	16: 87,487,678 (GRCm38)	M207K	probably benign	Het
Cnga1	A	G	5: 72,612,359 (GRCm38)		probably null	Het
Cst13	T	C	2: 148,828,191 (GRCm38)	C94R	probably damaging	Het
Cyp8b1	G	T	9: 121,915,798 (GRCm38)	S156*	probably null	Het
Dip2b	T	C	15: 100,151,276 (GRCm38)	S184P	probably damaging	Het
Dmbt1	T	C	7: 131,058,254 (GRCm38)	W516R	probably damaging	Het
Dock8	A	T	19: 25,095,550 (GRCm38)	Y398F	possibly damaging	Het
Drosha	T	A	15: 12,889,717 (GRCm38)	C890*	probably null	Het
Eed	A	G	7: 89,976,941 (GRCm38)	S75P	probably benign	Het
Etaa1	A	C	11: 17,946,833 (GRCm38)	I428S	probably benign	Het
F5	A	T	1: 164,193,493 (GRCm38)	D1179V	probably damaging	Het
Fat1	T	C	8: 44,952,342 (GRCm38)	V710A	possibly damaging	Het
Fmo4	G	A	1: 162,793,969 (GRCm38)	Q558*	probably null	Het
Gm11639	T	C	11: 104,994,317 (GRCm38)	L4134S	possibly damaging	Het
Gm7361	A	G	5: 26,258,962 (GRCm38)	I72V	probably benign	Het
Grm4	A	G	17: 27,435,320 (GRCm38)	V552A	probably benign	Het
Krt13	T	A	11: 100,119,376 (GRCm38)	I260F	probably damaging	Het
Krtap3-3	T	C	11: 99,550,664 (GRCm38)	D49G	probably damaging	Het
Lpin1	T	C	12: 16,564,553 (GRCm38)	E409G	probably benign	Het
Ly9	A	G	1: 171,601,008 (GRCm38)	V238A	possibly damaging	Het
Map2k6	T	C	11: 110,490,877 (GRCm38)		probably null	Het
Mylk2	T	C	2: 152,917,395 (GRCm38)	L362P	probably damaging	Het
Olfr1225	T	A	2: 89,170,598 (GRCm38)	I205F	probably benign	Het
Olfr1264	A	G	2: 90,021,631 (GRCm38)	V145A	probably benign	Het
Olfr575	T	A	7: 102,955,415 (GRCm38)	Y69F	possibly damaging	Het
Pcdha9	G	A	18: 36,997,919 (GRCm38)	V14M	probably benign	Het
Pdzrn4	A	T	15: 92,680,537 (GRCm38)	E380D	probably damaging	Het
Piezo2	A	G	18: 63,106,293 (GRCm38)	Y739H	possibly damaging	Het
Pigk	A	G	3: 152,740,849 (GRCm38)	H195R	probably benign	Het
Plin2	T	C	4: 86,661,999 (GRCm38)	D175G	probably null	Het
Plk4	T	A	3: 40,808,973 (GRCm38)	H526Q	probably benign	Het
Pom121l12	A	T	11: 14,599,489 (GRCm38)	D65V	probably damaging	Het
Prb1	T	C	6: 132,207,176 (GRCm38)	Y498C	unknown	Het
Sh3bp2	G	A	5: 34,561,603 (GRCm38)	V495I	probably damaging	Het
Slx4ip	T	A	2: 137,046,749 (GRCm38)	C117S	probably damaging	Het
Tmtc2	A	T	10: 105,573,690 (GRCm38)	S20R	probably benign	Het
Unc79	A	G	12: 103,021,010 (GRCm38)	Y186C	probably damaging	Het
Vmn2r106	C	T	17: 20,268,463 (GRCm38)	C558Y	probably damaging	Het
Wdr17	A	T	8: 54,661,460 (GRCm38)	S674T	probably benign	Het
Zfp959	T	C	17: 55,895,854 (GRCm38)	F10L	probably damaging	Het

Other mutations in Vmn2r26

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01070:Vmn2r26	APN	6	124,061,607 (GRCm38)	missense	probably benign	0.00
IGL01370:Vmn2r26	APN	6	124,061,756 (GRCm38)	missense	probably benign	0.08
IGL01603:Vmn2r26	APN	6	124,053,874 (GRCm38)	missense	probably damaging	1.00
IGL01651:Vmn2r26	APN	6	124,050,673 (GRCm38)	missense	probably benign	0.01
IGL02282:Vmn2r26	APN	6	124,061,625 (GRCm38)	missense	probably damaging	1.00
IGL02425:Vmn2r26	APN	6	124,061,818 (GRCm38)	missense	probably damaging	1.00
IGL02551:Vmn2r26	APN	6	124,026,141 (GRCm38)	missense	probably benign	0.11
IGL02690:Vmn2r26	APN	6	124,026,132 (GRCm38)	missense	probably benign	0.14
IGL03002:Vmn2r26	APN	6	124,039,795 (GRCm38)	missense	possibly damaging	0.78
IGL03270:Vmn2r26	APN	6	124,050,819 (GRCm38)	missense	probably benign	0.16
R0032:Vmn2r26	UTSW	6	124,039,899 (GRCm38)	missense	possibly damaging	0.72
R0052:Vmn2r26	UTSW	6	124,062,033 (GRCm38)	makesense	probably null
R0083:Vmn2r26	UTSW	6	124,053,981 (GRCm38)	splice site	probably null
R0682:Vmn2r26	UTSW	6	124,061,170 (GRCm38)	missense	probably damaging	0.97
R1061:Vmn2r26	UTSW	6	124,061,644 (GRCm38)	missense	probably benign	0.12
R1077:Vmn2r26	UTSW	6	124,053,913 (GRCm38)	missense	probably benign	0.00
R1263:Vmn2r26	UTSW	6	124,050,708 (GRCm38)	missense	probably benign
R1579:Vmn2r26	UTSW	6	124,039,747 (GRCm38)	missense	probably benign	0.00
R1741:Vmn2r26	UTSW	6	124,061,472 (GRCm38)	missense	probably damaging	1.00
R1834:Vmn2r26	UTSW	6	124,061,410 (GRCm38)	missense	possibly damaging	0.54
R1838:Vmn2r26	UTSW	6	124,024,771 (GRCm38)	missense	probably benign
R1956:Vmn2r26	UTSW	6	124,053,887 (GRCm38)	missense	probably damaging	1.00
R1996:Vmn2r26	UTSW	6	124,061,185 (GRCm38)	missense	probably damaging	1.00
R2140:Vmn2r26	UTSW	6	124,061,237 (GRCm38)	missense	probably benign	0.01
R2327:Vmn2r26	UTSW	6	124,039,749 (GRCm38)	missense	probably benign	0.07
R2417:Vmn2r26	UTSW	6	124,061,350 (GRCm38)	missense	probably damaging	1.00
R3930:Vmn2r26	UTSW	6	124,025,979 (GRCm38)	missense	probably benign
R4490:Vmn2r26	UTSW	6	124,050,738 (GRCm38)	missense	possibly damaging	0.47
R4629:Vmn2r26	UTSW	6	124,061,191 (GRCm38)	missense	possibly damaging	0.50
R4655:Vmn2r26	UTSW	6	124,061,416 (GRCm38)	missense	probably damaging	1.00
R4709:Vmn2r26	UTSW	6	124,053,965 (GRCm38)	missense	probably damaging	1.00
R4992:Vmn2r26	UTSW	6	124,026,111 (GRCm38)	missense	probably benign	0.00
R5297:Vmn2r26	UTSW	6	124,061,873 (GRCm38)	missense	probably damaging	1.00
R5482:Vmn2r26	UTSW	6	124,061,326 (GRCm38)	missense	possibly damaging	0.88
R5517:Vmn2r26	UTSW	6	124,050,717 (GRCm38)	missense	probably damaging	1.00
R5737:Vmn2r26	UTSW	6	124,039,449 (GRCm38)	missense	probably benign	0.00
R5739:Vmn2r26	UTSW	6	124,025,966 (GRCm38)	missense	probably benign	0.00
R5873:Vmn2r26	UTSW	6	124,061,674 (GRCm38)	missense	probably benign	0.01
R5907:Vmn2r26	UTSW	6	124,039,871 (GRCm38)	missense	probably benign	0.00
R6086:Vmn2r26	UTSW	6	124,039,560 (GRCm38)	missense	possibly damaging	0.48
R6134:Vmn2r26	UTSW	6	124,061,485 (GRCm38)	missense	probably damaging	0.97
R6428:Vmn2r26	UTSW	6	124,026,080 (GRCm38)	missense	probably benign	0.17
R6637:Vmn2r26	UTSW	6	124,061,691 (GRCm38)	missense	probably damaging	1.00
R6927:Vmn2r26	UTSW	6	124,039,098 (GRCm38)	missense	possibly damaging	0.93
R6953:Vmn2r26	UTSW	6	124,039,782 (GRCm38)	missense	probably benign	0.00
R7173:Vmn2r26	UTSW	6	124,061,296 (GRCm38)	missense	probably benign	0.16
R7206:Vmn2r26	UTSW	6	124,039,768 (GRCm38)	missense	probably benign	0.17
R7208:Vmn2r26	UTSW	6	124,061,989 (GRCm38)	missense	probably damaging	1.00
R7283:Vmn2r26	UTSW	6	124,025,955 (GRCm38)	missense	probably damaging	0.97
R7506:Vmn2r26	UTSW	6	124,039,741 (GRCm38)	missense	probably benign	0.00
R7672:Vmn2r26	UTSW	6	124,039,647 (GRCm38)	missense	probably benign	0.25
R7674:Vmn2r26	UTSW	6	124,039,362 (GRCm38)	missense	probably benign
R7696:Vmn2r26	UTSW	6	124,061,535 (GRCm38)	missense	possibly damaging	0.94
R7716:Vmn2r26	UTSW	6	124,061,745 (GRCm38)	missense	probably damaging	1.00
R7831:Vmn2r26	UTSW	6	124,039,799 (GRCm38)	nonsense	probably null
R8063:Vmn2r26	UTSW	6	124,024,955 (GRCm38)	missense	probably benign	0.00
R8331:Vmn2r26	UTSW	6	124,061,928 (GRCm38)	missense	probably benign	0.22
R8352:Vmn2r26	UTSW	6	124,039,618 (GRCm38)	missense	probably benign	0.09
R8445:Vmn2r26	UTSW	6	124,026,036 (GRCm38)	missense	probably damaging	0.97
R8452:Vmn2r26	UTSW	6	124,039,618 (GRCm38)	missense	probably benign	0.09
R8681:Vmn2r26	UTSW	6	124,024,918 (GRCm38)	missense	probably benign	0.00
R8914:Vmn2r26	UTSW	6	124,062,024 (GRCm38)	missense	probably benign
R9333:Vmn2r26	UTSW	6	124,026,050 (GRCm38)	missense	probably benign	0.13
R9351:Vmn2r26	UTSW	6	124,039,374 (GRCm38)	missense	probably benign
R9436:Vmn2r26	UTSW	6	124,025,867 (GRCm38)	missense	probably damaging	1.00
R9515:Vmn2r26	UTSW	6	124,061,178 (GRCm38)	missense	probably damaging	1.00
RF010:Vmn2r26	UTSW	6	124,039,489 (GRCm38)	missense	possibly damaging	0.90

Predicted Primers

PCR Primer
(F):5'- AACAGAGGAATCAGTGTCTCC -3'
(R):5'- GCATTTGAGAGTCGGGTCAC -3'

Sequencing Primer
(F):5'- CCCTAAAATTATGGTATTTTTGGCCC -3'
(R):5'- ATTTGAGAGTCGGGTCACCATCC -3'

Posted On

2018-05-04