Mutagenetix > Incidental Mutation

Incidental Mutation 'R6401:Vmn2r112'

516180

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r112

Ensembl Gene

ENSMUSG00000094921

Gene Name

vomeronasal 2, receptor 112

Synonyms

EG628185

MMRRC Submission

044548-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.105) question?

Stock #

R6401 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

22820129-22838114 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 22822532 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 403 (Y403*)

Ref Sequence

ENSEMBL: ENSMUSP00000094994 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000097381]

AlphaFold

L7N221

Predicted Effect

probably null
Transcript: ENSMUST00000097381
AA Change: Y403*

SMART Domains

Protein: ENSMUSP00000094994
Gene: ENSMUSG00000094921
AA Change: Y403*

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	73	471	2.8e-32	PFAM
Pfam:NCD3G	512	565	5.8e-21	PFAM
Pfam:7tm_3	598	833	6.5e-54	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 97.9%

Validation Efficiency

93% (41/44)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ano3	T	A	2: 110,605,459 (GRCm39)	N249I	probably benign	Het
Ap3s1	A	C	18: 46,891,074 (GRCm39)	I56L	probably benign	Het
Ccn5	T	C	2: 163,670,946 (GRCm39)	I151T	probably benign	Het
Cngb1	A	G	8: 96,030,367 (GRCm39)		probably benign	Het
Col2a1	T	C	15: 97,883,773 (GRCm39)	T570A	unknown	Het
Cyp3a16	T	C	5: 145,377,174 (GRCm39)	E471G	probably damaging	Het
D930020B18Rik	A	G	10: 121,477,762 (GRCm39)	N14D	possibly damaging	Het
Ext1	T	C	15: 52,969,493 (GRCm39)	E365G	possibly damaging	Het
Fbn1	C	A	2: 125,188,370 (GRCm39)	V1490F	probably damaging	Het
Fsip2	A	G	2: 82,820,430 (GRCm39)	T5388A	possibly damaging	Het
Gm5431	T	A	11: 48,779,536 (GRCm39)	N740I	probably benign	Het
Ifi209	T	C	1: 173,472,269 (GRCm39)	M370T	probably damaging	Het
Ighv2-4	G	T	12: 113,617,082 (GRCm39)	P60Q	probably damaging	Het
Kif19b	A	T	5: 140,442,698 (GRCm39)	T80S	possibly damaging	Het
Ldb3	A	T	14: 34,299,291 (GRCm39)	L111Q	probably benign	Het
Mcmbp	G	T	7: 128,308,783 (GRCm39)	L413I	possibly damaging	Het
Mib1	T	A	18: 10,795,802 (GRCm39)	M721K	probably benign	Het
Nos3	A	G	5: 24,584,809 (GRCm39)	T738A	probably benign	Het
Notch3	T	A	17: 32,377,597 (GRCm39)	I160L	probably benign	Het
Nrxn3	C	A	12: 89,221,770 (GRCm39)	N516K	possibly damaging	Het
Nt5c2	A	G	19: 46,878,250 (GRCm39)	Y496H	probably benign	Het
Or13f5	A	C	4: 52,826,242 (GRCm39)	T282P	probably damaging	Het
Or1n1	A	T	2: 36,750,177 (GRCm39)	L61*	probably null	Het
Or5b111	T	C	19: 13,290,878 (GRCm39)	Y257C	probably damaging	Het
Polm	A	T	11: 5,779,491 (GRCm39)	W436R	probably damaging	Het
Prex2	T	A	1: 11,256,951 (GRCm39)	I1221N	probably benign	Het
Rfpl4b	T	C	10: 38,696,941 (GRCm39)	H220R	possibly damaging	Het
Rgs12	T	C	5: 35,177,676 (GRCm39)	F79L	probably damaging	Het
Rxfp2	A	G	5: 149,966,595 (GRCm39)	D111G	probably benign	Het
Smg7	A	G	1: 152,715,887 (GRCm39)		probably null	Het
Spata22	T	C	11: 73,224,180 (GRCm39)	S34P	probably damaging	Het
St7	T	A	6: 17,855,317 (GRCm39)		probably null	Het
Stk31	A	G	6: 49,400,372 (GRCm39)	E399G	probably damaging	Het
Tcp10b	C	A	17: 13,292,466 (GRCm39)	N296K	probably damaging	Het
Tonsl	T	C	15: 76,517,866 (GRCm39)	Y645C	probably damaging	Het
Ttn	A	T	2: 76,800,206 (GRCm39)	M334K	probably benign	Het
Vcpkmt	C	A	12: 69,629,619 (GRCm39)	V48F	probably damaging	Het
Vwa7	G	A	17: 35,236,286 (GRCm39)		probably null	Het
Wscd2	A	T	5: 113,726,206 (GRCm39)	*572C	probably null	Het
Xpo7	A	G	14: 70,919,787 (GRCm39)	L676P	probably damaging	Het
Zbtb32	A	C	7: 30,291,244 (GRCm39)	L17W	probably damaging	Het

Other mutations in Vmn2r112

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00341:Vmn2r112	APN	17	22,837,917 (GRCm39)	missense	probably benign	0.13
IGL01021:Vmn2r112	APN	17	22,837,885 (GRCm39)	missense	probably damaging	1.00
IGL01122:Vmn2r112	APN	17	22,821,988 (GRCm39)	missense	probably benign	0.00
IGL01360:Vmn2r112	APN	17	22,837,603 (GRCm39)	missense	probably benign	0.03
IGL01536:Vmn2r112	APN	17	22,824,136 (GRCm39)	missense	probably damaging	1.00
IGL02148:Vmn2r112	APN	17	22,838,013 (GRCm39)	missense	probably damaging	1.00
IGL02465:Vmn2r112	APN	17	22,833,975 (GRCm39)	missense	probably damaging	1.00
PIT4576001:Vmn2r112	UTSW	17	22,833,912 (GRCm39)	missense	probably benign	0.00
R0278:Vmn2r112	UTSW	17	22,821,987 (GRCm39)	missense	probably benign	0.44
R0328:Vmn2r112	UTSW	17	22,824,251 (GRCm39)	missense	probably benign	0.01
R0583:Vmn2r112	UTSW	17	22,837,930 (GRCm39)	missense	probably damaging	1.00
R0831:Vmn2r112	UTSW	17	22,833,980 (GRCm39)	missense	probably damaging	0.99
R1080:Vmn2r112	UTSW	17	22,837,980 (GRCm39)	missense	probably damaging	1.00
R1245:Vmn2r112	UTSW	17	22,822,228 (GRCm39)	missense	probably benign	0.03
R1321:Vmn2r112	UTSW	17	22,837,500 (GRCm39)	nonsense	probably null
R1381:Vmn2r112	UTSW	17	22,837,467 (GRCm39)	missense	probably damaging	1.00
R1514:Vmn2r112	UTSW	17	22,821,825 (GRCm39)	missense	probably benign	0.40
R1519:Vmn2r112	UTSW	17	22,837,884 (GRCm39)	missense	possibly damaging	0.83
R1572:Vmn2r112	UTSW	17	22,822,125 (GRCm39)	missense	possibly damaging	0.61
R1590:Vmn2r112	UTSW	17	22,833,989 (GRCm39)	critical splice donor site	probably null
R1640:Vmn2r112	UTSW	17	22,824,097 (GRCm39)	missense	probably benign	0.01
R2221:Vmn2r112	UTSW	17	22,820,214 (GRCm39)	missense	possibly damaging	0.86
R2223:Vmn2r112	UTSW	17	22,820,214 (GRCm39)	missense	possibly damaging	0.86
R2310:Vmn2r112	UTSW	17	22,822,096 (GRCm39)	missense	probably damaging	0.98
R2312:Vmn2r112	UTSW	17	22,822,096 (GRCm39)	missense	probably damaging	0.98
R2337:Vmn2r112	UTSW	17	22,822,096 (GRCm39)	missense	probably damaging	0.98
R2339:Vmn2r112	UTSW	17	22,822,096 (GRCm39)	missense	probably damaging	0.98
R2340:Vmn2r112	UTSW	17	22,822,096 (GRCm39)	missense	probably damaging	0.98
R2341:Vmn2r112	UTSW	17	22,822,096 (GRCm39)	missense	probably damaging	0.98
R2342:Vmn2r112	UTSW	17	22,822,096 (GRCm39)	missense	probably damaging	0.98
R2401:Vmn2r112	UTSW	17	22,822,096 (GRCm39)	missense	probably damaging	0.98
R2860:Vmn2r112	UTSW	17	22,822,096 (GRCm39)	missense	probably damaging	0.98
R2861:Vmn2r112	UTSW	17	22,822,096 (GRCm39)	missense	probably damaging	0.98
R2926:Vmn2r112	UTSW	17	22,833,984 (GRCm39)	missense	possibly damaging	0.90
R3236:Vmn2r112	UTSW	17	22,822,096 (GRCm39)	missense	probably damaging	0.98
R3237:Vmn2r112	UTSW	17	22,822,096 (GRCm39)	missense	probably damaging	0.98
R3977:Vmn2r112	UTSW	17	22,822,096 (GRCm39)	missense	probably damaging	0.98
R3979:Vmn2r112	UTSW	17	22,822,096 (GRCm39)	missense	probably damaging	0.98
R4168:Vmn2r112	UTSW	17	22,822,069 (GRCm39)	missense	probably benign	0.01
R4256:Vmn2r112	UTSW	17	22,837,393 (GRCm39)	missense	probably damaging	1.00
R4386:Vmn2r112	UTSW	17	22,820,303 (GRCm39)	missense	probably benign	0.36
R4912:Vmn2r112	UTSW	17	22,822,363 (GRCm39)	missense	probably damaging	0.99
R4947:Vmn2r112	UTSW	17	22,821,860 (GRCm39)	missense	probably benign	0.02
R5446:Vmn2r112	UTSW	17	22,837,231 (GRCm39)	missense	probably damaging	1.00
R5870:Vmn2r112	UTSW	17	22,838,004 (GRCm39)	missense	probably benign	0.00
R6351:Vmn2r112	UTSW	17	22,820,259 (GRCm39)	missense	probably benign
R6384:Vmn2r112	UTSW	17	22,824,136 (GRCm39)	missense	probably damaging	1.00
R6390:Vmn2r112	UTSW	17	22,824,230 (GRCm39)	missense	probably benign	0.01
R6405:Vmn2r112	UTSW	17	22,837,216 (GRCm39)	missense	probably damaging	1.00
R6620:Vmn2r112	UTSW	17	22,822,082 (GRCm39)	missense	probably benign	0.00
R6648:Vmn2r112	UTSW	17	22,837,467 (GRCm39)	missense	probably damaging	1.00
R6649:Vmn2r112	UTSW	17	22,820,160 (GRCm39)	missense	probably null	1.00
R6653:Vmn2r112	UTSW	17	22,820,160 (GRCm39)	missense	probably null	1.00
R6654:Vmn2r112	UTSW	17	22,822,450 (GRCm39)	missense	possibly damaging	0.89
R6700:Vmn2r112	UTSW	17	22,822,462 (GRCm39)	missense	possibly damaging	0.53
R6993:Vmn2r112	UTSW	17	22,822,195 (GRCm39)	missense	probably benign	0.01
R7052:Vmn2r112	UTSW	17	22,821,507 (GRCm39)	missense	probably benign
R7454:Vmn2r112	UTSW	17	22,822,288 (GRCm39)	missense	probably benign	0.00
R7763:Vmn2r112	UTSW	17	22,822,099 (GRCm39)	missense	probably damaging	1.00
R8032:Vmn2r112	UTSW	17	22,822,375 (GRCm39)	missense	probably benign	0.21
R8177:Vmn2r112	UTSW	17	22,822,594 (GRCm39)	missense	possibly damaging	0.47
R8263:Vmn2r112	UTSW	17	22,824,140 (GRCm39)	missense	probably damaging	1.00
R8395:Vmn2r112	UTSW	17	22,837,587 (GRCm39)	missense	possibly damaging	0.94
R8492:Vmn2r112	UTSW	17	22,821,470 (GRCm39)	missense	probably benign	0.03
R8889:Vmn2r112	UTSW	17	22,837,612 (GRCm39)	missense	probably damaging	1.00
R8892:Vmn2r112	UTSW	17	22,837,612 (GRCm39)	missense	probably damaging	1.00
R9246:Vmn2r112	UTSW	17	22,824,088 (GRCm39)	missense	probably benign	0.21
R9269:Vmn2r112	UTSW	17	22,820,213 (GRCm39)	missense	probably benign
R9273:Vmn2r112	UTSW	17	22,837,721 (GRCm39)	missense	probably damaging	1.00
R9288:Vmn2r112	UTSW	17	22,822,323 (GRCm39)	missense	probably damaging	1.00
R9352:Vmn2r112	UTSW	17	22,822,479 (GRCm39)	missense	probably damaging	0.98
R9406:Vmn2r112	UTSW	17	22,824,223 (GRCm39)	nonsense	probably null
R9432:Vmn2r112	UTSW	17	22,821,233 (GRCm39)	missense
R9728:Vmn2r112	UTSW	17	22,824,108 (GRCm39)	missense	probably damaging	0.96
Z1088:Vmn2r112	UTSW	17	22,824,059 (GRCm39)	missense	possibly damaging	0.89

Predicted Primers

PCR Primer
(F):5'- ATTGAACTCAGTCAAATGCCCAG -3'
(R):5'- AGGACAGATTATGCCTTCTAGTC -3'

Sequencing Primer
(F):5'- TCAGTCAAATGCCCAGATGAATATC -3'
(R):5'- ACATTTTCTAGCACACTTGGTTTGG -3'

Posted On

2018-05-04