Mutagenetix > Incidental Mutations

Incidental Mutation 'R6403:Iqcm'

516219

Institutional Source

Beutler Lab

Gene Symbol

Iqcm

Ensembl Gene

ENSMUSG00000031620

Gene Name

IQ motif containing M

Synonyms

1700007B14Rik

MMRRC Submission

044382-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R6403 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

75448694-75984503 bp(+) (GRCm38)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

T to C at 75577996 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000148495 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034033] [ENSMUST00000121983] [ENSMUST00000212704]

AlphaFold

Q149I8

Predicted Effect

probably null
Transcript: ENSMUST00000034033

SMART Domains

Protein: ENSMUSP00000034033
Gene: ENSMUSG00000031620

Domain	Start	End	E-Value	Type
IQ	281	303	2.54e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000121983

SMART Domains

Protein: ENSMUSP00000112901
Gene: ENSMUSG00000031620

Domain	Start	End	E-Value	Type
IQ	281	303	2.54e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000212704

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 27 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aars	A	G	8: 111,042,249	D227G	possibly damaging	Het
Aox1	A	G	1: 58,068,435	I623V	probably damaging	Het
Camsap2	G	A	1: 136,280,800	R317*	probably null	Het
Carmil1	G	A	13: 24,081,967	R271C	probably damaging	Het
Ces2b	T	A	8: 104,836,269	L323*	probably null	Het
Copb1	T	A	7: 114,238,451	I286F	probably damaging	Het
Dnajb11	T	A	16: 22,870,941	V285D	probably damaging	Het
Dok5	G	T	2: 170,829,900	A79S	probably damaging	Het
Enpp2	T	C	15: 54,863,764	N557S	probably damaging	Het
Gm13088	G	A	4: 143,655,773	Q118*	probably null	Het
Hexa	G	A	9: 59,557,361	R178H	probably damaging	Het
Iars	T	C	13: 49,687,495	S35P	probably damaging	Het
Il3ra	G	T	14: 14,347,137	A3S	probably damaging	Het
Krt9	G	T	11: 100,189,659	S420R	probably damaging	Het
Lca5l	T	C	16: 96,173,845	N293S	probably benign	Het
Mb21d2	A	G	16: 28,828,517	I235T	possibly damaging	Het
Ncbp3	A	G	11: 73,078,976	T550A	probably benign	Het
Nckipsd	G	T	9: 108,811,683	R139L	possibly damaging	Het
Nxn	A	G	11: 76,399,020	V14A	probably benign	Het
Olfr926	T	C	9: 38,877,242	L22P	probably damaging	Het
Rprd2	C	A	3: 95,766,087	C668F	possibly damaging	Het
Tmem87a	A	T	2: 120,380,771	M231K	possibly damaging	Het
Trappc8	A	G	18: 20,866,071	V333A	probably benign	Het
Trit1	C	A	4: 123,039,579	T103K	possibly damaging	Het
Tspan13	A	G	12: 36,015,705	F203S	probably damaging	Het
Vmn2r114	C	T	17: 23,309,965	D388N	probably damaging	Het
Zfp617	C	T	8: 71,929,171	A55V	probably benign	Het

Other mutations in Iqcm

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01895:Iqcm	APN	8	75888560	missense	probably damaging	1.00
IGL02835:Iqcm	APN	8	75554883	utr 5 prime	probably benign
R0056:Iqcm	UTSW	8	75753386	missense	probably benign
R2146:Iqcm	UTSW	8	75888613	missense	probably damaging	1.00
R2910:Iqcm	UTSW	8	75714776	missense	probably benign
R3801:Iqcm	UTSW	8	75669393	missense	possibly damaging	0.59
R3804:Iqcm	UTSW	8	75669393	missense	possibly damaging	0.59
R3834:Iqcm	UTSW	8	75577752	missense	possibly damaging	0.93
R3897:Iqcm	UTSW	8	75753400	missense	probably damaging	1.00
R4447:Iqcm	UTSW	8	75629766	missense	probably damaging	0.97
R4448:Iqcm	UTSW	8	75629766	missense	probably damaging	0.97
R4450:Iqcm	UTSW	8	75629766	missense	probably damaging	0.97
R4687:Iqcm	UTSW	8	75762989	missense	probably damaging	1.00
R4810:Iqcm	UTSW	8	75888653	missense	probably damaging	1.00
R4845:Iqcm	UTSW	8	75746352	missense	probably damaging	0.99
R4856:Iqcm	UTSW	8	75888600	missense	possibly damaging	0.95
R4886:Iqcm	UTSW	8	75888600	missense	possibly damaging	0.95
R5063:Iqcm	UTSW	8	75746286	missense	probably damaging	1.00
R5460:Iqcm	UTSW	8	75714789	missense	probably benign
R6667:Iqcm	UTSW	8	75753352	missense	probably damaging	1.00
R7187:Iqcm	UTSW	8	75753416	missense	probably benign	0.22
R7263:Iqcm	UTSW	8	75763073	missense	probably benign
R7701:Iqcm	UTSW	8	75554911	missense	probably benign	0.02
R7916:Iqcm	UTSW	8	75577950	missense	probably benign
R7938:Iqcm	UTSW	8	75577968	missense	probably benign
R7974:Iqcm	UTSW	8	75554892	start codon destroyed	probably null	0.66
R8039:Iqcm	UTSW	8	75763105	missense	probably damaging	1.00
R8703:Iqcm	UTSW	8	75888643	missense	probably damaging	1.00
R9175:Iqcm	UTSW	8	75984239	missense	possibly damaging	0.84
RF002:Iqcm	UTSW	8	75577899	missense	probably benign	0.01
X0018:Iqcm	UTSW	8	75984212	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ACTGTCAAGTTCATACCTTTTGAGG -3'
(R):5'- ACACAACAGCCAGTTCTGG -3'

Sequencing Primer
(F):5'- TCAAGTTCATACCTTTTGAGGTTAAG -3'
(R):5'- AGAATGCTCTCATCACAGTTTCTGG -3'

Posted On

2018-05-04