Mutagenetix > Incidental Mutation

Incidental Mutation 'R6406:Trim61'

516305

Institutional Source

Beutler Lab

Gene Symbol

Trim61

Ensembl Gene

ENSMUSG00000109718

Gene Name

tripartite motif-containing 61

Synonyms

Rnf35, E330039K03Rik, 2czf61

MMRRC Submission

044551-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.100) question?

Stock #

R6406 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

65465639-65471175 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 65466377 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 295 (T295A)

Ref Sequence

ENSEMBL: ENSMUSP00000077513 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000048565] [ENSMUST00000078409]

AlphaFold

Q8JZK6

Predicted Effect

probably benign
Transcript: ENSMUST00000048565

SMART Domains

Protein: ENSMUSP00000040299
Gene: ENSMUSG00000053490

Domain	Start	End	E-Value	Type
RING	15	55	1.48e-7	SMART
low complexity region	81	90	N/A	INTRINSIC
BBOX	91	132	3.12e-6	SMART
PRY	289	341	4.11e-15	SMART
Pfam:SPRY	344	459	2e-14	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000078409
AA Change: T295A

PolyPhen 2 Score 0.609 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000077513
Gene: ENSMUSG00000109718
AA Change: T295A

Domain	Start	End	E-Value	Type
RING	16	56	4.66e-5	SMART
low complexity region	157	170	N/A	INTRINSIC
coiled coil region	201	232	N/A	INTRINSIC
PRY	280	331	1.35e-10	SMART
Pfam:SPRY	334	458	5.7e-17	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000148141

Predicted Effect

probably benign
Transcript: ENSMUST00000211310

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000211739

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.3%

Validation Efficiency

96% (48/50)

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aars2	C	T	17: 45,817,865 (GRCm39)	P29L	probably benign	Het
Acsm1	A	G	7: 119,261,484 (GRCm39)	N567S	probably benign	Het
Ahi1	T	G	10: 20,852,948 (GRCm39)	N505K	probably damaging	Het
Ank2	T	A	3: 126,825,874 (GRCm39)	D356V	probably damaging	Het
Bhlhe40	TG	TGG	6: 108,641,818 (GRCm39)	254	probably null	Het
Bpifb6	C	A	2: 153,746,457 (GRCm39)	T117K	possibly damaging	Het
C2cd6	T	C	1: 59,097,835 (GRCm39)	E418G	possibly damaging	Het
Cbx1	A	T	11: 96,692,364 (GRCm39)	K84*	probably null	Het
Cr2	A	G	1: 194,852,079 (GRCm39)	L90P	probably damaging	Het
Dclk1	A	G	3: 55,387,827 (GRCm39)	D91G	probably damaging	Het
Dock1	C	A	7: 134,747,215 (GRCm39)	Q1509K	probably benign	Het
Fzd1	T	G	5: 4,806,089 (GRCm39)	T498P	probably damaging	Het
Galnt12	A	G	4: 47,122,534 (GRCm39)	N271S	probably benign	Het
Gm12888	A	G	4: 121,176,654 (GRCm39)	I49T	possibly damaging	Het
Gria4	A	T	9: 4,427,077 (GRCm39)	W788R	probably damaging	Het
Ilf3	C	T	9: 21,307,540 (GRCm39)	A379V	probably damaging	Het
Islr2	A	G	9: 58,107,263 (GRCm39)	V43A	probably benign	Het
Jag1	T	A	2: 136,929,563 (GRCm39)	N782I	probably damaging	Het
Klhl8	T	A	5: 104,010,981 (GRCm39)	I539F	possibly damaging	Het
Lama5	A	T	2: 179,839,257 (GRCm39)	C750*	probably null	Het
Lrrc37a	T	A	11: 103,388,361 (GRCm39)	T2355S	unknown	Het
Lrrc8a	A	G	2: 30,147,103 (GRCm39)	H639R	possibly damaging	Het
Map1a	A	T	2: 121,131,224 (GRCm39)	D442V	probably damaging	Het
Mier3	T	C	13: 111,846,343 (GRCm39)		probably null	Het
Msantd1	C	A	5: 35,080,665 (GRCm39)		probably null	Het
Ncapd2	C	A	6: 125,150,841 (GRCm39)	A785S	probably benign	Het
Ncbp2	CTCGTCTGG	C	16: 31,775,159 (GRCm39)		probably null	Het
Ncbp2	CGTCTGGATG	CG	16: 31,775,161 (GRCm39)		probably null	Het
Ndst4	T	C	3: 125,232,150 (GRCm39)	S240P	probably benign	Het
Nek9	T	C	12: 85,385,946 (GRCm39)	D17G	probably damaging	Het
Nkx6-1	C	T	5: 101,811,677 (GRCm39)	A142T	unknown	Het
Nt5dc1	T	C	10: 34,200,404 (GRCm39)	H205R	probably benign	Het
Or5b94	T	C	19: 12,652,184 (GRCm39)	I205T	probably benign	Het
Or9g3	T	A	2: 85,590,651 (GRCm39)	Q23L	possibly damaging	Het
Pafah1b1	A	G	11: 74,573,098 (GRCm39)	M322T	probably benign	Het
Parp2	A	G	14: 51,056,934 (GRCm39)	N353D	probably benign	Het
Pdcd6ip	T	C	9: 113,503,412 (GRCm39)	N452S	possibly damaging	Het
Pkd1l2	A	G	8: 117,762,586 (GRCm39)	V1397A	probably damaging	Het
Prkdc	T	A	16: 15,535,665 (GRCm39)	L1675Q	probably damaging	Het
Ptprt	T	C	2: 161,395,703 (GRCm39)	I1157V	probably damaging	Het
Rsf1	CGGCGGCGG	CGGCGGCGGGGGCGGCGG	7: 97,229,133 (GRCm39)		probably benign	Het
Sele	G	T	1: 163,878,312 (GRCm39)	C217F	probably damaging	Het
Spats1	T	A	17: 45,768,191 (GRCm39)	H125L	probably damaging	Het
Stard3	T	C	11: 98,269,595 (GRCm39)	V330A	probably benign	Het
Synj2	T	A	17: 6,069,846 (GRCm39)		probably benign	Het
Thoc1	T	A	18: 9,977,963 (GRCm39)	F301L	probably damaging	Het
Thumpd3	G	A	6: 113,032,924 (GRCm39)	E221K	probably damaging	Het
Trp53bp1	T	C	2: 121,101,093 (GRCm39)	Q35R	probably damaging	Het
Tsnaxip1	C	T	8: 106,570,615 (GRCm39)	T578I	probably benign	Het

Other mutations in Trim61

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00793:Trim61	APN	8	65,466,743 (GRCm39)	missense	possibly damaging	0.94
R0184:Trim61	UTSW	8	65,467,069 (GRCm39)	missense	probably benign	0.40
R1955:Trim61	UTSW	8	65,466,044 (GRCm39)	missense	possibly damaging	0.46
R3928:Trim61	UTSW	8	65,465,969 (GRCm39)	missense	probably benign	0.39
R3929:Trim61	UTSW	8	65,465,969 (GRCm39)	missense	probably benign	0.39
R4850:Trim61	UTSW	8	65,466,070 (GRCm39)	missense	probably damaging	1.00
R6249:Trim61	UTSW	8	65,467,108 (GRCm39)	missense	probably benign	0.25
R6390:Trim61	UTSW	8	65,466,842 (GRCm39)	missense	probably benign	0.24
R7159:Trim61	UTSW	8	65,466,526 (GRCm39)	missense	probably benign	0.19
R7182:Trim61	UTSW	8	65,466,266 (GRCm39)	missense	probably damaging	0.99
R9359:Trim61	UTSW	8	65,467,228 (GRCm39)	missense	probably damaging	1.00
R9403:Trim61	UTSW	8	65,467,228 (GRCm39)	missense	probably damaging	1.00
X0022:Trim61	UTSW	8	65,465,965 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- TCATCAGAGAACCGTTCGGC -3'
(R):5'- TGGCAAGTGTTAAAGATTGGTACG -3'

Sequencing Primer
(F):5'- CATACCTGCTCCTGGGAAAAGG -3'
(R):5'- TTGGTACGAGAGATACAAACAGTC -3'

Posted On

2018-05-04