Mutagenetix > Incidental Mutation

Incidental Mutation 'R6466:Or5p5'

516582

Institutional Source

Beutler Lab

Gene Symbol

Or5p5

Ensembl Gene

ENSMUSG00000066242

Gene Name

olfactory receptor family 5 subfamily P member 5

Synonyms

MOR204-33P, Olfr467, GA_x6K02T2PBJ9-10144091-10145011

MMRRC Submission

044599-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.103) question?

Stock #

R6466 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

107413793-107414713 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 107413901 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 37 (T37A)

Ref Sequence

ENSEMBL: ENSMUSP00000149295 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000084756] [ENSMUST00000208563] [ENSMUST00000214253]

AlphaFold

L7N455

Predicted Effect

probably benign
Transcript: ENSMUST00000084756
AA Change: T39A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000081811
Gene: ENSMUSG00000066242
AA Change: T39A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	33	308	5.3e-52	PFAM
Pfam:7TM_GPCR_Srsx	37	307	8.3e-7	PFAM
Pfam:7tm_1	43	292	2.2e-23	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000207203

Predicted Effect

probably benign
Transcript: ENSMUST00000208563
AA Change: T37A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Predicted Effect

probably benign
Transcript: ENSMUST00000214253
AA Change: T37A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Coding Region Coverage

1x: 99.9%
3x: 99.4%
10x: 97.3%
20x: 91.1%

Validation Efficiency

98% (56/57)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1190005I06Rik	A	T	8: 121,335,735 (GRCm39)	D69E	probably damaging	Het
AAdacl4fm3	T	A	4: 144,430,437 (GRCm39)	D184V	probably damaging	Het
Adgrv1	G	T	13: 81,723,220 (GRCm39)		probably null	Het
Ahr	A	T	12: 35,554,031 (GRCm39)	V696E	probably benign	Het
Akap13	A	G	7: 75,376,792 (GRCm39)	T2007A	probably benign	Het
Arid1b	T	C	17: 5,377,953 (GRCm39)	F753S	probably damaging	Het
Bpifb3	G	T	2: 153,764,108 (GRCm39)	K105N	probably damaging	Het
C2cd2	A	G	16: 97,680,822 (GRCm39)	C331R	probably benign	Het
Chrm1	T	A	19: 8,655,542 (GRCm39)	Y82*	probably null	Het
Clcn3	A	C	8: 61,382,595 (GRCm39)	V331G	probably damaging	Het
Dchs1	A	T	7: 105,413,748 (GRCm39)	D1022E	probably benign	Het
Dnah2	T	A	11: 69,430,241 (GRCm39)	T106S	probably benign	Het
Fam171a2	T	A	11: 102,330,711 (GRCm39)	D256V	probably damaging	Het
Firrm	G	T	1: 163,781,734 (GRCm39)	R898S	probably benign	Het
Fmn2	T	A	1: 174,437,149 (GRCm39)		probably benign	Het
Fut11	C	T	14: 20,745,377 (GRCm39)	R103W	probably damaging	Het
Gas2l2	A	C	11: 83,320,179 (GRCm39)	S26A	probably damaging	Het
Gramd1a	A	T	7: 30,843,221 (GRCm39)	I29N	probably benign	Het
Grem2	A	G	1: 174,664,450 (GRCm39)	V133A	probably damaging	Het
Hydin	T	A	8: 111,233,600 (GRCm39)	S1813T	possibly damaging	Het
Igkv3-2	T	C	6: 70,676,023 (GRCm39)	F111L	probably benign	Het
Ints8	T	C	4: 11,252,878 (GRCm39)	Q68R	probably damaging	Het
Irx5	A	G	8: 93,086,354 (GRCm39)	I146V	probably damaging	Het
Kcnh3	T	C	15: 99,136,124 (GRCm39)	L707P	probably damaging	Het
Kcnk4	T	A	19: 6,905,665 (GRCm39)	I101F	probably damaging	Het
Klhl9	A	G	4: 88,639,399 (GRCm39)	Y281H	probably benign	Het
Klra9	G	T	6: 130,155,995 (GRCm39)	Y253*	probably null	Het
Lmbr1	C	T	5: 29,583,166 (GRCm39)	A9T	probably benign	Het
Map10	G	T	8: 126,399,123 (GRCm39)	E839*	probably null	Het
Nectin4	C	A	1: 171,214,321 (GRCm39)	A492D	probably damaging	Het
Nfat5	T	A	8: 108,082,140 (GRCm39)		probably null	Het
Plec	A	G	15: 76,062,084 (GRCm39)	Y2608H	probably benign	Het
Pold3	A	G	7: 99,749,839 (GRCm39)	S42P	probably benign	Het
Ppp2r1a	G	T	17: 21,180,893 (GRCm39)	G432*	probably null	Het
Qki	T	C	17: 10,434,394 (GRCm39)	E315G	probably benign	Het
Rfx2	C	T	17: 57,091,397 (GRCm39)	V354I	probably benign	Het
Rp1	T	C	1: 4,418,109 (GRCm39)	Y1001C	probably benign	Het
Sez6l	A	G	5: 112,609,007 (GRCm39)		probably null	Het
Slc39a14	A	G	14: 70,547,335 (GRCm39)	I337T	probably damaging	Het
Slc3a2	G	T	19: 8,686,683 (GRCm39)	L76M	probably damaging	Het
Slco1a5	T	C	6: 142,183,260 (GRCm39)	T555A	probably benign	Het
Sprr2e	A	T	3: 92,260,341 (GRCm39)	K57N	unknown	Het
Syne2	A	G	12: 75,990,675 (GRCm39)	T1886A	probably damaging	Het
Tenm3	A	T	8: 48,689,098 (GRCm39)	I2147N	probably damaging	Het
Thbs1	G	T	2: 117,950,328 (GRCm39)	G654W	probably damaging	Het
Tigd5	A	G	15: 75,782,352 (GRCm39)	Y238C	possibly damaging	Het
Tmem221	A	G	8: 72,010,493 (GRCm39)	F126S	probably damaging	Het
Trp63	C	A	16: 25,582,108 (GRCm39)	P52Q	probably damaging	Het
Ugp2	A	T	11: 21,278,883 (GRCm39)	S434R	probably benign	Het
Vmn2r69	A	C	7: 85,056,378 (GRCm39)	F587V	probably benign	Het
Vps13d	T	A	4: 144,784,065 (GRCm39)	N3872I	possibly damaging	Het
Wdfy2	A	G	14: 63,186,115 (GRCm39)	Y250C	probably damaging	Het
Zbtb16	T	C	9: 48,576,619 (GRCm39)	D487G	possibly damaging	Het

Other mutations in Or5p5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0110:Or5p5	UTSW	7	107,413,895 (GRCm39)	missense	probably damaging	1.00
R0450:Or5p5	UTSW	7	107,413,895 (GRCm39)	missense	probably damaging	1.00
R0707:Or5p5	UTSW	7	107,414,331 (GRCm39)	missense	probably damaging	0.99
R0918:Or5p5	UTSW	7	107,414,418 (GRCm39)	missense	probably benign	0.03
R1416:Or5p5	UTSW	7	107,414,469 (GRCm39)	missense	probably damaging	1.00
R1988:Or5p5	UTSW	7	107,413,907 (GRCm39)	missense	probably benign
R1989:Or5p5	UTSW	7	107,413,907 (GRCm39)	missense	probably benign
R2219:Or5p5	UTSW	7	107,414,429 (GRCm39)	missense	probably benign
R2241:Or5p5	UTSW	7	107,414,040 (GRCm39)	missense	possibly damaging	0.62
R2866:Or5p5	UTSW	7	107,414,126 (GRCm39)	missense	probably benign	0.06
R4946:Or5p5	UTSW	7	107,414,589 (GRCm39)	missense	possibly damaging	0.90
R4972:Or5p5	UTSW	7	107,413,953 (GRCm39)	missense	probably benign	0.10
R5099:Or5p5	UTSW	7	107,413,809 (GRCm39)	missense	probably benign	0.00
R5310:Or5p5	UTSW	7	107,414,171 (GRCm39)	missense	probably damaging	1.00
R5323:Or5p5	UTSW	7	107,413,883 (GRCm39)	missense	possibly damaging	0.96
R5531:Or5p5	UTSW	7	107,414,451 (GRCm39)	missense	probably benign	0.03
R5672:Or5p5	UTSW	7	107,413,844 (GRCm39)	missense	probably damaging	1.00
R5758:Or5p5	UTSW	7	107,414,022 (GRCm39)	missense	probably damaging	0.99
R5891:Or5p5	UTSW	7	107,414,387 (GRCm39)	missense	probably damaging	1.00
R6016:Or5p5	UTSW	7	107,414,219 (GRCm39)	missense	probably benign	0.01
R6399:Or5p5	UTSW	7	107,413,961 (GRCm39)	missense	possibly damaging	0.78
R6894:Or5p5	UTSW	7	107,414,271 (GRCm39)	missense	probably benign	0.25
R7543:Or5p5	UTSW	7	107,414,308 (GRCm39)	missense	probably damaging	1.00
R8316:Or5p5	UTSW	7	107,414,030 (GRCm39)	missense	possibly damaging	0.81
R9285:Or5p5	UTSW	7	107,413,821 (GRCm39)	missense	probably damaging	0.96

Predicted Primers

PCR Primer
(F):5'- AAATGTGGAGTGGTGTATGTCAATC -3'
(R):5'- ACATCAGAGCCAAGCTGGAC -3'

Sequencing Primer
(F):5'- AGTGGTGTATGTCAATCATGAGG -3'
(R):5'- TCAGAGCCAAGCTGGACTATGC -3'

Posted On

2018-05-21