Mutagenetix > Incidental Mutation

Incidental Mutation 'R6478:Trio'

516937

Institutional Source

Beutler Lab

Gene Symbol

Trio

Ensembl Gene

ENSMUSG00000022263

Gene Name

triple functional domain (PTPRF interacting)

Synonyms

Solo, 6720464I07Rik

MMRRC Submission

044610-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6478 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

27730651-28025848 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 27856107 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 666 (V666A)

Ref Sequence

ENSEMBL: ENSMUSP00000154653 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090247] [ENSMUST00000227337]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000090247
AA Change: V725A

PolyPhen 2 Score 0.455 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000087714
Gene: ENSMUSG00000022263
AA Change: V725A

Domain	Start	End	E-Value	Type
low complexity region	2	40	N/A	INTRINSIC
SEC14	68	207	3.4e-26	SMART
SPEC	221	337	2.48e-9	SMART
SPEC	343	445	1.92e-15	SMART
SPEC	569	671	5.35e-14	SMART
SPEC	674	783	1.18e-6	SMART
SPEC	910	1011	2.6e-12	SMART
SPEC	1141	1243	7e-18	SMART
low complexity region	1249	1258	N/A	INTRINSIC
RhoGEF	1296	1466	2.79e-53	SMART
PH	1480	1593	1.53e-9	SMART
SH3	1659	1720	1.9e-8	SMART
low complexity region	1788	1802	N/A	INTRINSIC
low complexity region	1837	1863	N/A	INTRINSIC
low complexity region	1936	1954	N/A	INTRINSIC
RhoGEF	1973	2144	1.32e-63	SMART
PH	2158	2273	3.6e-6	SMART
low complexity region	2291	2341	N/A	INTRINSIC
low complexity region	2371	2390	N/A	INTRINSIC
low complexity region	2491	2503	N/A	INTRINSIC
SH3	2558	2619	1.04e0	SMART
low complexity region	2640	2660	N/A	INTRINSIC
IGc2	2701	2770	4e-12	SMART
S_TKc	2800	3054	4.84e-72	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000226580

Predicted Effect

probably benign
Transcript: ENSMUST00000227337
AA Change: V666A

PolyPhen 2 Score 0.013 (Sensitivity: 0.96; Specificity: 0.78)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000228765

Meta Mutation Damage Score

0.0717

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 97.8%
20x: 93.3%

Validation Efficiency

95% (75/79)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a large protein that functions as a GDP to GTP exchange factor. This protein promotes the reorganization of the actin cytoskeleton, thereby playing a role in cell migration and growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
PHENOTYPE: Homozygous mutant mice die during late embryonic development or shortly after birth. They exhibit abnormal skeletal myogenesis and display aberrant organization within the hippocampus and olfactory bulb. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3632451O06Rik	A	G	14: 49,773,332 (GRCm38)	V306A	possibly damaging	Het
9530053A07Rik	C	G	7: 28,155,373 (GRCm38)	P1808R	probably damaging	Het
Aatk	T	A	11: 120,010,991 (GRCm38)	S803C	probably benign	Het
Abcc9	C	T	6: 142,679,308 (GRCm38)	A454T	probably damaging	Het
Abr	T	C	11: 76,452,332 (GRCm38)	E565G	probably damaging	Het
Adam33	C	A	2: 131,051,346 (GRCm38)	R753L	probably benign	Het
Adgre1	A	G	17: 57,401,955 (GRCm38)	T49A	possibly damaging	Het
AI314180	A	T	4: 58,810,785 (GRCm38)	I1524N	probably damaging	Het
Ankra2	A	T	13: 98,268,442 (GRCm38)	H153L	probably damaging	Het
Ankrd26	T	G	6: 118,511,638 (GRCm38)	E1353D	probably benign	Het
Ankrd52	T	G	10: 128,379,331 (GRCm38)		probably null	Het
Arhgef10l	T	A	4: 140,542,757 (GRCm38)	T619S	possibly damaging	Het
Asgr1	T	C	11: 70,056,894 (GRCm38)	V130A	possibly damaging	Het
Atg10	C	A	13: 90,937,347 (GRCm38)	C161F	probably damaging	Het
Atm	A	T	9: 53,490,254 (GRCm38)	N1438K	probably damaging	Het
BC107364	T	A	3: 96,436,006 (GRCm38)		probably null	Het
Bcar3	G	T	3: 122,426,576 (GRCm38)	A41S	probably benign	Het
Btbd6	A	G	12: 112,977,312 (GRCm38)		probably benign	Het
Cchcr1	A	G	17: 35,524,703 (GRCm38)	T318A	possibly damaging	Het
Celsr1	A	T	15: 85,925,518 (GRCm38)	I2221N	probably damaging	Het
Cept1	C	T	3: 106,533,445 (GRCm38)	W48*	probably null	Het
Cfap161	T	A	7: 83,793,276 (GRCm38)	I85L	probably benign	Het
Clasp1	A	T	1: 118,512,180 (GRCm38)	R640*	probably null	Het
Col5a1	T	A	2: 27,952,436 (GRCm38)	I441N	unknown	Het
Col9a1	C	A	1: 24,185,405 (GRCm38)	L223I	unknown	Het
Dnah2	T	C	11: 69,516,010 (GRCm38)	D223G	probably benign	Het
Dnah8	G	A	17: 30,748,568 (GRCm38)	D2585N	probably benign	Het
Dpy19l1	A	T	9: 24,450,696 (GRCm38)	M129K	possibly damaging	Het
Fcrl1	C	A	3: 87,389,639 (GRCm38)	H318Q	probably benign	Het
Fer1l5	A	G	1: 36,402,531 (GRCm38)	N609S	probably damaging	Het
Fkbp4	C	T	6: 128,433,231 (GRCm38)	E256K	probably damaging	Het
Fsip2	A	G	2: 82,990,086 (GRCm38)	T5388A	possibly damaging	Het
Glrx	G	A	13: 75,847,299 (GRCm38)		probably null	Het
Gm6614	A	T	6: 141,993,642 (GRCm38)	N208K	possibly damaging	Het
Gm7356	A	T	17: 14,001,464 (GRCm38)	M101K	probably damaging	Het
Gstp2	A	T	19: 4,040,499 (GRCm38)	I162N	probably benign	Het
Hectd3	A	C	4: 116,999,586 (GRCm38)	K443N	probably damaging	Het
Hmcn1	T	A	1: 150,664,784 (GRCm38)	R2925W	probably damaging	Het
Hspa1a	G	T	17: 34,970,306 (GRCm38)	N540K	probably damaging	Het
Ints6	A	T	14: 62,700,786 (GRCm38)	M649K	probably benign	Het
Katnb1	T	C	8: 95,095,456 (GRCm38)	V270A	possibly damaging	Het
Kctd3	A	G	1: 188,972,364 (GRCm38)	S737P	probably benign	Het
Klra10	T	C	6: 130,272,544 (GRCm38)		probably null	Het
Lmtk3	A	G	7: 45,798,589 (GRCm38)	D1353G	unknown	Het
Lrrk1	A	G	7: 66,262,733 (GRCm38)	V1693A	probably damaging	Het
Mpnd	A	T	17: 56,009,575 (GRCm38)	I85F	probably damaging	Het
Myo3b	A	G	2: 70,348,960 (GRCm38)	T1173A	probably benign	Het
Myof	G	A	19: 37,903,831 (GRCm38)	P1158L	probably damaging	Het
Naip2	A	C	13: 100,162,041 (GRCm38)	S496A	probably benign	Het
Ncor2	A	G	5: 125,110,005 (GRCm38)		probably benign	Het
Npepps	C	T	11: 97,258,273 (GRCm38)		probably null	Het
Olfr1247	T	G	2: 89,609,446 (GRCm38)	I219L	probably damaging	Het
Opn5	T	A	17: 42,580,749 (GRCm38)	I266F	probably benign	Het
P2rx4	A	G	5: 122,707,700 (GRCm38)	D16G	probably damaging	Het
Padi2	G	T	4: 140,917,637 (GRCm38)	V61L	probably benign	Het
Pkd1l1	G	A	11: 8,863,911 (GRCm38)	T1480I	probably benign	Het
Plcb1	T	C	2: 135,335,451 (GRCm38)	S568P	probably damaging	Het
Rassf10	A	G	7: 112,955,707 (GRCm38)	E505G	probably damaging	Het
Rcan2	A	G	17: 43,836,334 (GRCm38)	E21G	probably benign	Het
Rhob	G	T	12: 8,499,585 (GRCm38)	C16*	probably null	Het
Ryr3	T	C	2: 112,660,068 (GRCm38)	N3807S	probably damaging	Het
Sass6	T	A	3: 116,621,397 (GRCm38)	N519K	probably benign	Het
Smad9	A	T	3: 54,782,443 (GRCm38)	D28V	probably damaging	Het
Tepp	A	T	8: 95,321,266 (GRCm38)		probably null	Het
Tex14	G	T	11: 87,514,373 (GRCm38)	G704C	probably benign	Het
Tmc3	A	G	7: 83,622,316 (GRCm38)	N892S	probably benign	Het
Tnfaip2	T	A	12: 111,445,663 (GRCm38)	L166Q	probably damaging	Het
Triml2	A	G	8: 43,185,128 (GRCm38)		probably null	Het
Tshz2	G	T	2: 169,884,664 (GRCm38)	L393F	probably damaging	Het
Ttn	T	G	2: 76,841,771 (GRCm38)		probably benign	Het
Tubb5	A	G	17: 35,835,842 (GRCm38)	Y159H	probably damaging	Het
Umodl1	A	G	17: 30,959,155 (GRCm38)	Y35C	probably damaging	Het
Utp4	T	C	8: 106,904,446 (GRCm38)		probably null	Het
Vmn1r60	A	T	7: 5,544,865 (GRCm38)	C79S	probably damaging	Het
Wnt5b	T	C	6: 119,433,790 (GRCm38)	T230A	probably damaging	Het
Zfp618	A	T	4: 63,132,706 (GRCm38)	I575F	probably damaging	Het
Zmym6	G	T	4: 127,123,383 (GRCm38)	V894L	possibly damaging	Het
Zpbp	T	C	11: 11,462,318 (GRCm38)		probably benign	Het

Other mutations in Trio

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00480:Trio	APN	15	27,912,743 (GRCm38)	splice site	probably benign
IGL01011:Trio	APN	15	27,736,489 (GRCm38)	missense	probably damaging	0.96
IGL01090:Trio	APN	15	27,773,007 (GRCm38)	missense	probably damaging	1.00
IGL01145:Trio	APN	15	27,818,167 (GRCm38)	splice site	probably benign
IGL01147:Trio	APN	15	27,881,320 (GRCm38)	missense	probably damaging	1.00
IGL01161:Trio	APN	15	27,749,781 (GRCm38)	missense	probably damaging	1.00
IGL01324:Trio	APN	15	27,905,323 (GRCm38)	missense	probably benign	0.42
IGL01352:Trio	APN	15	27,901,229 (GRCm38)	missense	probably benign	0.01
IGL01366:Trio	APN	15	27,732,868 (GRCm38)	missense	possibly damaging	0.76
IGL01443:Trio	APN	15	27,838,775 (GRCm38)	splice site	probably benign
IGL01454:Trio	APN	15	27,832,985 (GRCm38)	missense	probably benign	0.32
IGL01695:Trio	APN	15	27,773,001 (GRCm38)	missense	probably damaging	1.00
IGL01765:Trio	APN	15	27,764,026 (GRCm38)	missense	possibly damaging	0.85
IGL01860:Trio	APN	15	27,846,810 (GRCm38)	missense	probably damaging	1.00
IGL01879:Trio	APN	15	27,741,033 (GRCm38)	missense	probably benign	0.12
IGL01991:Trio	APN	15	27,871,274 (GRCm38)	missense	possibly damaging	0.95
IGL02106:Trio	APN	15	27,744,158 (GRCm38)	missense	possibly damaging	0.85
IGL02209:Trio	APN	15	27,744,053 (GRCm38)	missense	probably damaging	1.00
IGL02232:Trio	APN	15	27,902,561 (GRCm38)	missense	probably benign	0.24
IGL02304:Trio	APN	15	27,735,436 (GRCm38)	missense	probably damaging	0.96
IGL02504:Trio	APN	15	27,847,390 (GRCm38)	nonsense	probably null
IGL02508:Trio	APN	15	27,818,104 (GRCm38)	missense	possibly damaging	0.65
IGL02541:Trio	APN	15	27,844,930 (GRCm38)	splice site	probably benign
IGL02617:Trio	APN	15	27,841,849 (GRCm38)	splice site	probably benign
IGL02675:Trio	APN	15	27,768,039 (GRCm38)	unclassified	probably benign
IGL02817:Trio	APN	15	27,902,881 (GRCm38)	missense	probably benign	0.01
IGL02993:Trio	APN	15	27,830,239 (GRCm38)	splice site	probably benign
IGL03007:Trio	APN	15	27,902,742 (GRCm38)	missense	probably damaging	0.99
IGL03135:Trio	APN	15	27,832,011 (GRCm38)	splice site	probably benign
IGL03225:Trio	APN	15	27,902,695 (GRCm38)	missense	probably benign	0.30
R0063:Trio	UTSW	15	27,881,437 (GRCm38)	splice site	probably benign
R0063:Trio	UTSW	15	27,881,437 (GRCm38)	splice site	probably benign
R0302:Trio	UTSW	15	27,902,517 (GRCm38)	missense	probably damaging	1.00
R0505:Trio	UTSW	15	27,767,907 (GRCm38)	missense	probably benign	0.00
R0506:Trio	UTSW	15	27,854,963 (GRCm38)	missense	probably benign	0.12
R0564:Trio	UTSW	15	27,805,822 (GRCm38)	missense	probably damaging	1.00
R0659:Trio	UTSW	15	27,831,399 (GRCm38)	missense	probably damaging	0.97
R0882:Trio	UTSW	15	27,732,894 (GRCm38)	missense	probably damaging	1.00
R0939:Trio	UTSW	15	27,741,250 (GRCm38)	critical splice donor site	probably null
R1018:Trio	UTSW	15	27,871,171 (GRCm38)	missense	probably damaging	1.00
R1439:Trio	UTSW	15	27,897,914 (GRCm38)	missense	probably damaging	1.00
R1456:Trio	UTSW	15	27,753,804 (GRCm38)	splice site	probably benign
R1488:Trio	UTSW	15	27,740,967 (GRCm38)	missense	probably damaging	1.00
R1522:Trio	UTSW	15	27,732,640 (GRCm38)	missense	probably benign	0.28
R1531:Trio	UTSW	15	27,832,985 (GRCm38)	missense	probably benign	0.32
R1640:Trio	UTSW	15	27,833,044 (GRCm38)	missense	probably damaging	1.00
R1646:Trio	UTSW	15	27,758,347 (GRCm38)	missense	possibly damaging	0.91
R1682:Trio	UTSW	15	27,744,146 (GRCm38)	splice site	probably null
R1780:Trio	UTSW	15	27,744,038 (GRCm38)	missense	possibly damaging	0.93
R1791:Trio	UTSW	15	27,841,756 (GRCm38)	missense	probably damaging	1.00
R1803:Trio	UTSW	15	27,748,340 (GRCm38)	missense	probably benign
R1817:Trio	UTSW	15	27,742,495 (GRCm38)	nonsense	probably null
R1853:Trio	UTSW	15	27,756,536 (GRCm38)	missense	probably damaging	1.00
R1898:Trio	UTSW	15	27,742,380 (GRCm38)	missense	possibly damaging	0.52
R1937:Trio	UTSW	15	27,833,056 (GRCm38)	missense	probably damaging	1.00
R1938:Trio	UTSW	15	27,732,891 (GRCm38)	missense	probably damaging	0.98
R2025:Trio	UTSW	15	27,773,927 (GRCm38)	missense	probably damaging	1.00
R2025:Trio	UTSW	15	27,744,137 (GRCm38)	missense	probably damaging	0.99
R2050:Trio	UTSW	15	27,851,945 (GRCm38)	missense	possibly damaging	0.85
R2186:Trio	UTSW	15	27,823,975 (GRCm38)	splice site	probably null
R2913:Trio	UTSW	15	27,854,912 (GRCm38)	missense	probably damaging	1.00
R3151:Trio	UTSW	15	27,805,776 (GRCm38)	missense	probably damaging	1.00
R3771:Trio	UTSW	15	27,748,091 (GRCm38)	missense	probably damaging	0.98
R3773:Trio	UTSW	15	27,748,091 (GRCm38)	missense	probably damaging	0.98
R3826:Trio	UTSW	15	27,833,070 (GRCm38)	missense	probably damaging	1.00
R4015:Trio	UTSW	15	27,744,101 (GRCm38)	missense	possibly damaging	0.71
R4359:Trio	UTSW	15	27,749,797 (GRCm38)	nonsense	probably null
R4370:Trio	UTSW	15	27,748,337 (GRCm38)	nonsense	probably null
R4547:Trio	UTSW	15	27,818,982 (GRCm38)	missense	possibly damaging	0.89
R4573:Trio	UTSW	15	27,772,998 (GRCm38)	small deletion	probably benign
R4620:Trio	UTSW	15	27,871,171 (GRCm38)	missense	probably damaging	1.00
R4735:Trio	UTSW	15	27,752,789 (GRCm38)	splice site	probably null
R4764:Trio	UTSW	15	27,732,538 (GRCm38)	nonsense	probably null
R4775:Trio	UTSW	15	27,881,342 (GRCm38)	nonsense	probably null
R4942:Trio	UTSW	15	27,752,725 (GRCm38)	missense	probably benign	0.21
R5004:Trio	UTSW	15	27,755,178 (GRCm38)	missense	probably damaging	1.00
R5149:Trio	UTSW	15	27,754,029 (GRCm38)	missense	possibly damaging	0.74
R5183:Trio	UTSW	15	27,902,600 (GRCm38)	missense	probably benign	0.00
R5186:Trio	UTSW	15	27,897,991 (GRCm38)	missense	probably damaging	0.97
R5268:Trio	UTSW	15	27,748,286 (GRCm38)	missense	probably benign	0.02
R5344:Trio	UTSW	15	27,735,532 (GRCm38)	missense	probably benign	0.12
R5407:Trio	UTSW	15	27,844,806 (GRCm38)	splice site	probably null
R5442:Trio	UTSW	15	27,856,194 (GRCm38)	missense	probably benign	0.04
R5617:Trio	UTSW	15	27,902,748 (GRCm38)	missense	probably benign
R5778:Trio	UTSW	15	27,856,164 (GRCm38)	missense	probably benign	0.33
R5986:Trio	UTSW	15	27,851,933 (GRCm38)	missense	possibly damaging	0.88
R5990:Trio	UTSW	15	27,891,459 (GRCm38)	missense	probably benign	0.10
R6011:Trio	UTSW	15	27,735,545 (GRCm38)	missense	probably damaging	0.98
R6063:Trio	UTSW	15	27,891,379 (GRCm38)	missense	possibly damaging	0.94
R6166:Trio	UTSW	15	27,818,071 (GRCm38)	missense	probably damaging	0.96
R6187:Trio	UTSW	15	27,743,952 (GRCm38)	critical splice donor site	probably null
R6387:Trio	UTSW	15	27,752,739 (GRCm38)	missense	probably damaging	1.00
R6402:Trio	UTSW	15	27,902,911 (GRCm38)	missense	probably benign	0.02
R6528:Trio	UTSW	15	27,805,870 (GRCm38)	missense	probably damaging	1.00
R6662:Trio	UTSW	15	27,854,996 (GRCm38)	missense	probably benign	0.00
R6825:Trio	UTSW	15	27,889,308 (GRCm38)	missense	probably damaging	0.98
R6890:Trio	UTSW	15	27,919,288 (GRCm38)	unclassified	probably benign
R6945:Trio	UTSW	15	27,824,090 (GRCm38)	missense	probably damaging	1.00
R7027:Trio	UTSW	15	27,805,654 (GRCm38)	missense	possibly damaging	0.86
R7046:Trio	UTSW	15	27,832,051 (GRCm38)	missense	probably damaging	1.00
R7049:Trio	UTSW	15	27,749,799 (GRCm38)	missense	possibly damaging	0.66
R7075:Trio	UTSW	15	27,898,000 (GRCm38)	missense	unknown
R7094:Trio	UTSW	15	27,891,448 (GRCm38)	missense	unknown
R7123:Trio	UTSW	15	27,742,313 (GRCm38)	critical splice donor site	probably benign
R7130:Trio	UTSW	15	27,742,313 (GRCm38)	critical splice donor site	probably benign
R7214:Trio	UTSW	15	27,871,187 (GRCm38)	missense	probably damaging	0.97
R7292:Trio	UTSW	15	27,828,351 (GRCm38)	missense	possibly damaging	0.63
R7293:Trio	UTSW	15	27,871,289 (GRCm38)	missense	possibly damaging	0.66
R7352:Trio	UTSW	15	27,732,876 (GRCm38)	missense	probably damaging	0.96
R7426:Trio	UTSW	15	27,856,107 (GRCm38)	missense	probably benign	0.01
R7451:Trio	UTSW	15	27,747,913 (GRCm38)	missense	probably benign	0.07
R7558:Trio	UTSW	15	27,831,394 (GRCm38)	missense	possibly damaging	0.90
R7578:Trio	UTSW	15	27,854,939 (GRCm38)	missense	possibly damaging	0.94
R7596:Trio	UTSW	15	27,749,826 (GRCm38)	missense	probably damaging	0.99
R7604:Trio	UTSW	15	27,736,445 (GRCm38)	critical splice donor site	probably null
R7609:Trio	UTSW	15	27,912,642 (GRCm38)	missense	unknown
R7767:Trio	UTSW	15	27,889,418 (GRCm38)	missense	unknown
R7784:Trio	UTSW	15	27,763,994 (GRCm38)	missense	probably damaging	1.00
R7817:Trio	UTSW	15	27,749,866 (GRCm38)	missense	probably benign	0.35
R7833:Trio	UTSW	15	27,774,086 (GRCm38)	missense	probably damaging	0.99
R7873:Trio	UTSW	15	27,805,684 (GRCm38)	missense	possibly damaging	0.83
R7879:Trio	UTSW	15	27,851,924 (GRCm38)	missense	possibly damaging	0.94
R7989:Trio	UTSW	15	27,772,935 (GRCm38)	missense	probably damaging	0.97
R8022:Trio	UTSW	15	27,749,866 (GRCm38)	missense	probably benign	0.35
R8050:Trio	UTSW	15	27,891,454 (GRCm38)	missense	unknown
R8217:Trio	UTSW	15	27,818,969 (GRCm38)	missense	probably damaging	0.97
R8280:Trio	UTSW	15	27,902,910 (GRCm38)	missense	unknown
R8283:Trio	UTSW	15	27,756,542 (GRCm38)	missense	possibly damaging	0.79
R8300:Trio	UTSW	15	27,855,022 (GRCm38)	missense	possibly damaging	0.66
R8321:Trio	UTSW	15	27,881,326 (GRCm38)	missense	possibly damaging	0.90
R8477:Trio	UTSW	15	27,773,952 (GRCm38)	missense	possibly damaging	0.83
R8479:Trio	UTSW	15	27,901,200 (GRCm38)	missense	probably benign	0.25
R8682:Trio	UTSW	15	27,905,192 (GRCm38)	missense	unknown
R8688:Trio	UTSW	15	27,748,238 (GRCm38)	missense	possibly damaging	0.61
R8708:Trio	UTSW	15	27,732,546 (GRCm38)	missense	probably damaging	0.99
R8709:Trio	UTSW	15	27,919,237 (GRCm38)	missense	unknown
R8713:Trio	UTSW	15	27,743,951 (GRCm38)	critical splice donor site	probably benign
R8798:Trio	UTSW	15	27,851,837 (GRCm38)	missense	possibly damaging	0.92
R8812:Trio	UTSW	15	27,905,225 (GRCm38)	missense	unknown
R8816:Trio	UTSW	15	27,741,271 (GRCm38)	missense	probably damaging	0.96
R8828:Trio	UTSW	15	27,741,064 (GRCm38)	missense	possibly damaging	0.93
R8987:Trio	UTSW	15	27,732,687 (GRCm38)	missense	probably benign	0.23
R9051:Trio	UTSW	15	27,732,684 (GRCm38)	missense	possibly damaging	0.78
R9069:Trio	UTSW	15	27,852,011 (GRCm38)	missense	possibly damaging	0.83
R9075:Trio	UTSW	15	27,773,936 (GRCm38)	nonsense	probably null
R9079:Trio	UTSW	15	27,732,937 (GRCm38)	missense	possibly damaging	0.52
R9139:Trio	UTSW	15	27,749,836 (GRCm38)	nonsense	probably null
R9494:Trio	UTSW	15	27,846,757 (GRCm38)	missense	probably benign	0.00
R9680:Trio	UTSW	15	27,744,072 (GRCm38)	missense	possibly damaging	0.93
R9720:Trio	UTSW	15	27,847,409 (GRCm38)	missense	probably benign	0.00
R9726:Trio	UTSW	15	27,912,666 (GRCm38)	missense	unknown
X0024:Trio	UTSW	15	27,765,726 (GRCm38)	missense	possibly damaging	0.91
Z1176:Trio	UTSW	15	27,771,387 (GRCm38)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GAAAACTCTCCCTGGCCTTCTG -3'
(R):5'- GTTGCTGGACTCTTCAGGAC -3'

Sequencing Primer
(F):5'- CCTTCTGGAGAGGACACTGGAAC -3'
(R):5'- GGACTCTTCAGGACGCTCTC -3'

Posted On

2018-05-21