Incidental Mutation 'R6480:Stk35'
ID 517019
Institutional Source Beutler Lab
Gene Symbol Stk35
Ensembl Gene ENSMUSG00000037885
Gene Name serine/threonine kinase 35
Synonyms 1700054C12Rik, CLIK1, CLP-36 interacting kinase
MMRRC Submission 044612-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R6480 (G1)
Quality Score 225.009
Status Validated
Chromosome 2
Chromosomal Location 129800517-129832287 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) C to A at 129810687 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Aspartic acid to Glutamic Acid at position 369 (D369E)
Ref Sequence ENSEMBL: ENSMUSP00000126541 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000165413] [ENSMUST00000166282]
AlphaFold Q80ZW0
Predicted Effect possibly damaging
Transcript: ENSMUST00000165413
AA Change: D369E

PolyPhen 2 Score 0.953 (Sensitivity: 0.79; Specificity: 0.95)
SMART Domains Protein: ENSMUSP00000126541
Gene: ENSMUSG00000037885
AA Change: D369E

DomainStartEndE-ValueType
low complexity region 51 69 N/A INTRINSIC
low complexity region 115 137 N/A INTRINSIC
low complexity region 190 206 N/A INTRINSIC
Pfam:Pkinase_Tyr 207 295 2e-6 PFAM
Pfam:Pkinase 207 531 8.6e-51 PFAM
Pfam:Pkinase_Tyr 304 532 2.1e-23 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000166282
SMART Domains Protein: ENSMUSP00000132862
Gene: ENSMUSG00000037885

DomainStartEndE-ValueType
low complexity region 51 69 N/A INTRINSIC
low complexity region 115 137 N/A INTRINSIC
low complexity region 190 206 N/A INTRINSIC
Pfam:Pkinase_Tyr 207 300 5.9e-7 PFAM
Pfam:Pkinase 207 311 1e-9 PFAM
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.5%
  • 10x: 97.8%
  • 20x: 93.4%
Validation Efficiency 98% (57/58)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a kinase that is predominantly found in the nucleus. However, it can interact with PDLIM1/CLP-36 in the cytoplasm and localize to actin stress fibers. The encoded protein may be a regulator of actin stress fibers in nonmuscle cells. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 59 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Arl8b T A 6: 108,815,049 (GRCm38) N90K possibly damaging Het
Art3 T C 5: 92,392,817 (GRCm38) F140L probably damaging Het
Ascc3 T A 10: 50,710,953 (GRCm38) M967K probably damaging Het
Ccne1 G A 7: 38,106,854 (GRCm38) probably benign Het
Cdc14b T C 13: 64,225,650 (GRCm38) probably null Het
Ceacam2 T A 7: 25,519,989 (GRCm38) E282V probably damaging Het
Clk4 T A 11: 51,270,546 (GRCm38) C86* probably null Het
Cul2 A C 18: 3,417,561 (GRCm38) K115T possibly damaging Het
Dhx29 A T 13: 112,953,788 (GRCm38) K800* probably null Het
Dlec1 T C 9: 119,147,690 (GRCm38) F1741S probably benign Het
Dnah12 A G 14: 26,872,455 (GRCm38) T3455A probably damaging Het
Dnah8 G A 17: 30,748,568 (GRCm38) D2585N probably benign Het
Drd4 A T 7: 141,294,793 (GRCm38) I366F possibly damaging Het
Fam169b T A 7: 68,353,718 (GRCm38) Y273* probably null Het
Fbn1 T A 2: 125,335,418 (GRCm38) Y1833F probably benign Het
Fsip2 A G 2: 82,990,086 (GRCm38) T5388A possibly damaging Het
Gjb6 T C 14: 57,124,442 (GRCm38) I121V probably benign Het
Glg1 T C 8: 111,197,706 (GRCm38) T217A possibly damaging Het
Igkv5-48 A G 6: 69,726,826 (GRCm38) S32P probably benign Het
Itgax C A 7: 128,148,599 (GRCm38) F1062L probably benign Het
Lct G A 1: 128,294,320 (GRCm38) T1494I probably damaging Het
Lilra6 G A 7: 3,912,933 (GRCm38) T309I probably damaging Het
Mical3 T C 6: 121,034,275 (GRCm38) T321A possibly damaging Het
Mmp23 T A 4: 155,652,341 (GRCm38) N104I probably damaging Het
Muc3 T C 5: 137,142,390 (GRCm38) Y131C Het
Naip2 A C 13: 100,162,041 (GRCm38) S496A probably benign Het
Ndufs2 A G 1: 171,236,698 (GRCm38) S348P probably damaging Het
Nepro T C 16: 44,727,075 (GRCm38) S52P probably damaging Het
Nlrp6 A G 7: 140,927,443 (GRCm38) E874G possibly damaging Het
Nsmce1 A G 7: 125,491,418 (GRCm38) V9A probably benign Het
Olfr13 T C 6: 43,174,066 (GRCm38) F27L probably benign Het
Olfr1458 T A 19: 13,102,474 (GRCm38) T277S probably benign Het
Olfr357 T C 2: 36,996,995 (GRCm38) F62L probably benign Het
Olfr788 A T 10: 129,472,721 (GRCm38) I10F possibly damaging Het
Per2 A T 1: 91,429,382 (GRCm38) probably null Het
Pgbd1 T C 13: 21,423,476 (GRCm38) I183V probably benign Het
Pik3c2g T C 6: 139,730,469 (GRCm38) V113A probably benign Het
Pipox A G 11: 77,882,648 (GRCm38) L259P probably damaging Het
Pkd1l3 C T 8: 109,638,387 (GRCm38) Q1124* probably null Het
Plcd3 A G 11: 103,074,931 (GRCm38) S492P possibly damaging Het
Rcor2 T G 19: 7,271,046 (GRCm38) M142R probably benign Het
Rwdd3 T C 3: 121,156,452 (GRCm38) E115G probably damaging Het
Slc13a3 T C 2: 165,408,898 (GRCm38) Y475C probably damaging Het
Slc14a2 A T 18: 78,159,082 (GRCm38) I611N possibly damaging Het
Slc25a24 G T 3: 109,136,301 (GRCm38) M91I probably damaging Het
Spg11 T C 2: 122,092,305 (GRCm38) S888G probably benign Het
Spta1 A T 1: 174,187,148 (GRCm38) probably null Het
Tanc1 T C 2: 59,807,642 (GRCm38) S889P probably damaging Het
Tbc1d10b T C 7: 127,198,878 (GRCm38) N697S probably damaging Het
Tcof1 A T 18: 60,814,780 (GRCm38) probably null Het
Tg T A 15: 66,671,311 (GRCm38) Y25N probably damaging Het
Thbs1 T C 2: 118,119,117 (GRCm38) C563R probably damaging Het
Tlr11 A G 14: 50,363,055 (GRCm38) S833G possibly damaging Het
Uggt2 T A 14: 119,057,564 (GRCm38) H550L probably benign Het
Utp20 A G 10: 88,755,186 (GRCm38) probably null Het
Wbp1l T A 19: 46,654,319 (GRCm38) L253Q probably damaging Het
Zfp433 T A 10: 81,720,244 (GRCm38) S161R possibly damaging Het
Zfp865 A G 7: 5,029,783 (GRCm38) T256A probably damaging Het
Zpr1 A G 9: 46,274,711 (GRCm38) D160G probably benign Het
Other mutations in Stk35
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00850:Stk35 APN 2 129,801,992 (GRCm38) missense probably damaging 1.00
IGL02609:Stk35 APN 2 129,801,801 (GRCm38) missense probably damaging 1.00
fingernails UTSW 2 129,810,935 (GRCm38) missense probably damaging 1.00
R2256_Stk35_331 UTSW 2 129,810,507 (GRCm38) nonsense probably null
skinned UTSW 2 129,811,235 (GRCm38) intron probably benign
R0045:Stk35 UTSW 2 129,800,568 (GRCm38) nonsense probably null
R0306:Stk35 UTSW 2 129,801,763 (GRCm38) nonsense probably null
R0784:Stk35 UTSW 2 129,810,802 (GRCm38) nonsense probably null
R1536:Stk35 UTSW 2 129,811,235 (GRCm38) intron probably benign
R2256:Stk35 UTSW 2 129,810,507 (GRCm38) nonsense probably null
R2507:Stk35 UTSW 2 129,801,515 (GRCm38) missense probably damaging 0.97
R2508:Stk35 UTSW 2 129,801,515 (GRCm38) missense probably damaging 0.97
R3848:Stk35 UTSW 2 129,800,736 (GRCm38) missense probably benign 0.13
R3872:Stk35 UTSW 2 129,810,575 (GRCm38) missense possibly damaging 0.82
R4466:Stk35 UTSW 2 129,801,516 (GRCm38) missense probably damaging 0.99
R5144:Stk35 UTSW 2 129,810,935 (GRCm38) missense probably damaging 1.00
R6267:Stk35 UTSW 2 129,810,888 (GRCm38) nonsense probably null
R6296:Stk35 UTSW 2 129,810,888 (GRCm38) nonsense probably null
R6807:Stk35 UTSW 2 129,801,653 (GRCm38) missense probably damaging 0.97
R7203:Stk35 UTSW 2 129,801,593 (GRCm38) missense probably benign
R7476:Stk35 UTSW 2 129,810,725 (GRCm38) missense probably damaging 1.00
R8505:Stk35 UTSW 2 129,801,729 (GRCm38) missense probably damaging 0.99
R8998:Stk35 UTSW 2 129,810,589 (GRCm38) missense probably damaging 1.00
R8999:Stk35 UTSW 2 129,810,589 (GRCm38) missense probably damaging 1.00
R9224:Stk35 UTSW 2 129,810,571 (GRCm38) missense probably damaging 1.00
R9760:Stk35 UTSW 2 129,800,685 (GRCm38) missense probably benign
Predicted Primers PCR Primer
(F):5'- TATGCTGAGGAGCCCTGCTATC -3'
(R):5'- TGTCCGCCTTGGCTGTATAG -3'

Sequencing Primer
(F):5'- AGGAGCCCTGCTATCTCTGG -3'
(R):5'- CTTCGGGAGCCATGTAGAAGTC -3'
Posted On 2018-05-21