Mutagenetix > Incidental Mutation

Incidental Mutation 'R6460:Or10d3'

517573

Institutional Source

Beutler Lab

Gene Symbol

Or10d3

Ensembl Gene

ENSMUSG00000050853

Gene Name

olfactory receptor family 10 subfamily D member 3

Synonyms

GA_x6K02T2PVTD-33247839-33246901, MOR224-9, Olfr958

MMRRC Submission

044595-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.153) question?

Stock #

R6460 (G1)

Quality Score

217.468

Status

Not validated

Chromosome

Chromosomal Location

39461227-39462165 bp(-) (GRCm39)

Type of Mutation

frame shift

DNA Base Change (assembly)

CAGAG to CAG at 39462088 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000149788 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000062545] [ENSMUST00000215505] [ENSMUST00000217227]

AlphaFold

Q8VEY3

Predicted Effect

probably null
Transcript: ENSMUST00000062545

SMART Domains

Protein: ENSMUSP00000049930
Gene: ENSMUSG00000050853

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	306	1.1e-49	PFAM
Pfam:7TM_GPCR_Srsx	36	236	1.5e-6	PFAM
Pfam:7tm_1	41	288	3.4e-21	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000215505

Predicted Effect

probably null
Transcript: ENSMUST00000217227

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 97.9%
20x: 93.8%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca7	A	T	10: 79,844,862 (GRCm39)	H1528L	probably benign	Het
Ablim1	A	G	19: 57,068,271 (GRCm39)	S263P	possibly damaging	Het
Ahnak2	T	C	12: 112,750,610 (GRCm39)	E104G	probably null	Het
Apof	T	A	10: 128,105,086 (GRCm39)	M80K	probably damaging	Het
Arfgef1	C	T	1: 10,283,285 (GRCm39)	R208H	probably damaging	Het
Arhgef33	A	G	17: 80,657,018 (GRCm39)		probably null	Het
Atxn2l	CCAGCAGCAGCAGCAGCAGC	CCAGCAGCAGCAGCAGC	7: 126,093,420 (GRCm39)		probably benign	Het
Cabcoco1	T	C	10: 68,352,211 (GRCm39)	K34E	probably damaging	Het
Col4a4	C	A	1: 82,444,253 (GRCm39)	G1338V	unknown	Het
Coq9	T	A	8: 95,579,814 (GRCm39)	D256E	probably damaging	Het
Dnajc18	A	T	18: 35,833,963 (GRCm39)	C41S	probably benign	Het
Dnajc6	A	G	4: 101,472,795 (GRCm39)	I307M	probably damaging	Het
Emg1	A	G	6: 124,688,870 (GRCm39)	V46A	probably damaging	Het
Eya3	A	G	4: 132,408,174 (GRCm39)	S157G	probably damaging	Het
Eya4	T	C	10: 23,027,910 (GRCm39)	N274S	probably benign	Het
Fan1	T	A	7: 64,022,234 (GRCm39)	N340Y	probably damaging	Het
Fat3	A	G	9: 15,878,296 (GRCm39)	V3395A	probably damaging	Het
Fchsd1	A	T	18: 38,092,897 (GRCm39)		probably null	Het
Gm4846	A	G	1: 166,325,082 (GRCm39)	V3A	probably benign	Het
Hecw2	T	A	1: 53,907,992 (GRCm39)		probably null	Het
Herc3	T	A	6: 58,867,108 (GRCm39)	I10N	probably damaging	Het
Hhatl	C	T	9: 121,618,588 (GRCm39)	R138H	probably benign	Het
Hspa9	A	T	18: 35,085,765 (GRCm39)	H35Q	probably benign	Het
Irgq	T	A	7: 24,233,115 (GRCm39)	S319T	probably benign	Het
Kif1b	T	C	4: 149,277,053 (GRCm39)	M1337V	probably benign	Het
Ksr2	T	A	5: 117,894,449 (GRCm39)		probably null	Het
Lrriq1	T	C	10: 103,036,559 (GRCm39)	I865V	probably damaging	Het
Map2k1	A	G	9: 64,094,577 (GRCm39)	L355P	probably damaging	Het
Muc16	A	G	9: 18,551,812 (GRCm39)	I4827T	probably benign	Het
Myh1	T	C	11: 67,112,202 (GRCm39)	V1752A	probably benign	Het
Nfatc2ip	A	G	7: 125,986,909 (GRCm39)	V282A	probably damaging	Het
Nrg1	T	A	8: 32,308,561 (GRCm39)	E485V	probably damaging	Het
Ofcc1	T	C	13: 40,441,455 (GRCm39)	D2G	probably damaging	Het
Pclo	T	C	5: 14,729,146 (GRCm39)		probably benign	Het
Pom121	T	C	5: 135,420,537 (GRCm39)	K295E	unknown	Het
Rb1	A	C	14: 73,515,894 (GRCm39)	I294R	probably benign	Het
Schip1	C	A	3: 68,402,227 (GRCm39)	S101R	probably benign	Het
Sec24c	T	A	14: 20,740,868 (GRCm39)	Y629N	probably damaging	Het
Shkbp1	T	A	7: 27,049,963 (GRCm39)	H305L	probably benign	Het
Spag9	T	C	11: 93,959,801 (GRCm39)	I187T	probably damaging	Het
Srp72	C	A	5: 77,135,838 (GRCm39)	T256K	probably damaging	Het
Stk32c	T	A	7: 138,685,190 (GRCm39)	N320I	probably damaging	Het
Stxbp4	A	T	11: 90,497,811 (GRCm39)	S163T	probably benign	Het
Sycp1	T	G	3: 102,832,569 (GRCm39)	Y199S	probably damaging	Het
Tpk1	T	C	6: 43,445,961 (GRCm39)	D159G	probably benign	Het
Trav21-dv12	C	T	14: 54,114,191 (GRCm39)	H104Y	probably benign	Het
Trip4	A	T	9: 65,788,302 (GRCm39)	Y48N	probably damaging	Het
Trmt10b	A	G	4: 45,314,322 (GRCm39)	T255A	possibly damaging	Het
Ttn	G	A	2: 76,747,232 (GRCm39)	Q4606*	probably null	Het
Vcan	T	A	13: 89,838,806 (GRCm39)	K2246M	possibly damaging	Het
Zfp438	C	A	18: 5,213,603 (GRCm39)	G452C	probably damaging	Het
Zfp54	T	A	17: 21,654,004 (GRCm39)	I166N	probably benign	Het
Zfp735	T	C	11: 73,602,478 (GRCm39)	V474A	probably benign	Het
Zfp831	G	T	2: 174,488,360 (GRCm39)	G1012W	possibly damaging	Het

Other mutations in Or10d3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0446:Or10d3	UTSW	9	39,461,747 (GRCm39)	missense	probably damaging	0.96
R0539:Or10d3	UTSW	9	39,461,593 (GRCm39)	missense	probably damaging	0.99
R1512:Or10d3	UTSW	9	39,461,390 (GRCm39)	missense	probably damaging	1.00
R1521:Or10d3	UTSW	9	39,462,080 (GRCm39)	missense	possibly damaging	0.65
R1652:Or10d3	UTSW	9	39,461,591 (GRCm39)	missense	probably benign	0.03
R2099:Or10d3	UTSW	9	39,461,963 (GRCm39)	missense	probably benign	0.17
R2252:Or10d3	UTSW	9	39,461,273 (GRCm39)	missense	probably damaging	0.99
R2911:Or10d3	UTSW	9	39,462,117 (GRCm39)	missense	possibly damaging	0.76
R3722:Or10d3	UTSW	9	39,461,418 (GRCm39)	missense	probably damaging	1.00
R5745:Or10d3	UTSW	9	39,461,987 (GRCm39)	missense	probably damaging	1.00
R6504:Or10d3	UTSW	9	39,461,574 (GRCm39)	missense	probably damaging	1.00
R7063:Or10d3	UTSW	9	39,461,411 (GRCm39)	missense	possibly damaging	0.87
R7162:Or10d3	UTSW	9	39,461,525 (GRCm39)	missense	probably damaging	1.00
R8674:Or10d3	UTSW	9	39,461,675 (GRCm39)	missense	probably damaging	1.00
R8748:Or10d3	UTSW	9	39,461,712 (GRCm39)	nonsense	probably null
R8748:Or10d3	UTSW	9	39,461,711 (GRCm39)	missense	possibly damaging	0.95
R8822:Or10d3	UTSW	9	39,461,812 (GRCm39)	missense	probably benign	0.11
R9529:Or10d3	UTSW	9	39,461,723 (GRCm39)	missense	probably benign	0.28
R9631:Or10d3	UTSW	9	39,461,508 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AGCTGGGAGACACAGTCTTG -3'
(R):5'- GCCAAACACACTGCCAGTTG -3'

Sequencing Primer
(F):5'- GGAGATAAGTCTGCTAAGTCCC -3'
(R):5'- CAGTTGCATTTCATTTTCTGATCATG -3'

Posted On

2018-05-21