Mutagenetix > Incidental Mutation

Incidental Mutation 'R6416:Tnc'

517914

Institutional Source

Beutler Lab

Gene Symbol

Tnc

Ensembl Gene

ENSMUSG00000028364

Gene Name

tenascin C

Synonyms

TN, TN-C, hexabrachion, tenascin-C, C130033P17Rik, cytotactin, Hxb

MMRRC Submission

044558-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6416 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

63959785-64047015 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 64007816 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 909 (I909T)

Ref Sequence

ENSEMBL: ENSMUSP00000103000 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000030056] [ENSMUST00000107371] [ENSMUST00000107372] [ENSMUST00000107377]

AlphaFold

Q80YX1

Predicted Effect

probably benign
Transcript: ENSMUST00000030056
AA Change: I909T

PolyPhen 2 Score 0.052 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000030056
Gene: ENSMUSG00000028364
AA Change: I909T

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	3.4e-4	SMART
FN3	1528	1606	1.55e-7	SMART
FN3	1617	1694	1.53e-6	SMART
FN3	1705	1782	7.75e-8	SMART
FBG	1797	2007	4.08e-124	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107371

SMART Domains

Protein: ENSMUSP00000102994
Gene: ENSMUSG00000028364

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
Pfam:hEGF	173	185	4e-4	PFAM
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107372
AA Change: I909T

PolyPhen 2 Score 0.052 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000102995
Gene: ENSMUSG00000028364
AA Change: I909T

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	2.75e0	SMART
FN3	1529	1608	3.4e-4	SMART
FN3	1619	1697	1.55e-7	SMART
FN3	1708	1785	1.53e-6	SMART
FN3	1796	1873	7.75e-8	SMART
FBG	1888	2098	4.08e-124	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107377
AA Change: I909T

PolyPhen 2 Score 0.052 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000103000
Gene: ENSMUSG00000028364
AA Change: I909T

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
low complexity region	121	138	N/A	INTRINSIC
EGF	189	217	1.87e1	SMART
EGF_like	220	248	3.5e1	SMART
EGF	251	280	4.89e0	SMART
EGF	283	311	3.23e0	SMART
EGF_like	314	342	2.98e1	SMART
EGF	345	373	1.87e1	SMART
EGF	376	404	3.97e0	SMART
EGF	407	435	8.52e0	SMART
EGF	438	466	3.01e0	SMART
EGF	469	497	3.46e0	SMART
EGF	500	528	3.71e0	SMART
EGF	531	559	4.32e-1	SMART
EGF	562	590	1.84e1	SMART
EGF	593	621	3.82e-2	SMART
FN3	623	701	8.9e-8	SMART
FN3	712	794	1.53e-6	SMART
FN3	803	884	7.23e-8	SMART
FN3	893	974	1.71e-9	SMART
FN3	985	1062	2.56e-8	SMART
FN3	1074	1152	8.58e-1	SMART
FN3	1165	1245	2.72e-3	SMART
FN3	1256	1334	5.36e-2	SMART
FN3	1347	1427	4.93e0	SMART
FN3	1438	1517	3.4e-4	SMART
FN3	1528	1606	1.55e-7	SMART
FN3	1617	1694	1.53e-6	SMART
FN3	1705	1782	7.75e-8	SMART
FBG	1797	2007	4.08e-124	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000135042

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141428

Meta Mutation Damage Score

0.5740

Coding Region Coverage

1x: 100.0%
3x: 99.8%
10x: 98.4%
20x: 94.6%

Validation Efficiency

99% (67/68)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration. [provided by RefSeq, Jul 2011]
PHENOTYPE: Mice homozygous for several different targeted mutations show variable behavioral and nervous system phenotypes such as abnormal circadian rhythm, anxiety behavior, novelty-induced activity, swimming, impaired synaptic plasticity, long term potentiation and serotonin and dopamine neurotransmission. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310061N02Rik	C	A	16: 88,707,891 (GRCm38)	R6L	unknown	Het
Anxa11	A	G	14: 25,874,270 (GRCm38)	Q235R	possibly damaging	Het
Ap2b1	T	A	11: 83,308,239 (GRCm38)	M1K	probably null	Het
Atl2	G	A	17: 79,850,223 (GRCm38)	T563I	probably benign	Het
Azin1	A	T	15: 38,492,343 (GRCm38)	S307R	possibly damaging	Het
Ccdc153	A	G	9: 44,245,780 (GRCm38)	T118A	probably benign	Het
Chac1	G	A	2: 119,353,534 (GRCm38)	V206I	probably damaging	Het
Chrm3	A	G	13: 9,877,662 (GRCm38)	V446A	probably benign	Het
Cped1	T	C	6: 22,123,649 (GRCm38)	F467S	probably damaging	Het
Csmd3	G	A	15: 48,673,560 (GRCm38)	P82L	probably damaging	Het
Ddx60	C	T	8: 61,998,681 (GRCm38)	H1202Y	probably benign	Het
Ddx60	T	A	8: 61,977,950 (GRCm38)	S840T	probably benign	Het
Dnah8	T	A	17: 30,765,635 (GRCm38)	N3102K	probably benign	Het
Dst	A	T	1: 34,116,128 (GRCm38)	K85M	probably damaging	Het
Ehbp1l1	T	C	19: 5,718,757 (GRCm38)	I839M	probably benign	Het
Fam184b	T	A	5: 45,537,653 (GRCm38)	M750L	probably benign	Het
Frem2	T	C	3: 53,572,378 (GRCm38)	T1965A	probably benign	Het
Ftl1	A	T	7: 45,459,210 (GRCm38)	D41E	probably benign	Het
Garem2	G	A	5: 30,116,737 (GRCm38)	W698*	probably null	Het
Glt8d2	T	C	10: 82,652,906 (GRCm38)	Y283C	probably damaging	Het
Hars	T	C	18: 36,773,590 (GRCm38)	E109G	possibly damaging	Het
Hspb9	T	C	11: 100,714,210 (GRCm38)	S121P	probably damaging	Het
Hus1b	A	T	13: 30,947,205 (GRCm38)	L157Q	probably damaging	Het
Hykk	A	G	9: 54,946,359 (GRCm38)	M322V	probably benign	Het
Igkv4-61	C	A	6: 69,417,154 (GRCm38)	A31S	possibly damaging	Het
Il17f	T	A	1: 20,777,907 (GRCm38)	M116L	probably benign	Het
Kif22	T	C	7: 127,028,932 (GRCm38)	K9E	possibly damaging	Het
Krt90	T	C	15: 101,559,244 (GRCm38)	E233G	probably benign	Het
Lipg	T	C	18: 74,957,236 (GRCm38)	M81V	probably benign	Het
Mocos	C	T	18: 24,701,456 (GRCm38)	S850L	probably damaging	Het
Mug2	T	A	6: 122,082,754 (GRCm38)	S1364T	probably damaging	Het
Neb	A	T	2: 52,185,328 (GRCm38)	N208K	probably benign	Het
Oca2	G	T	7: 56,328,767 (GRCm38)	R561L	probably benign	Het
Olfm5	A	G	7: 104,154,053 (GRCm38)	L401P	probably damaging	Het
Olfr109	T	A	17: 37,467,080 (GRCm38)	Y291*	probably null	Het
Olfr1238	C	A	2: 89,406,522 (GRCm38)	A186S	possibly damaging	Het
Olfr331	T	C	11: 58,502,340 (GRCm38)	D72G	probably damaging	Het
Olfr828	G	A	9: 18,815,892 (GRCm38)	T134M	probably benign	Het
Olfr952	T	A	9: 39,426,891 (GRCm38)	Y60F	probably damaging	Het
Oxgr1	T	C	14: 120,022,448 (GRCm38)	N116D	probably damaging	Het
Pcdha11	G	T	18: 37,012,169 (GRCm38)		probably null	Het
Pi4ka	T	C	16: 17,358,322 (GRCm38)	I418V	probably benign	Het
Pih1d2	T	C	9: 50,618,609 (GRCm38)	V62A	probably benign	Het
Pkib	T	A	10: 57,728,138 (GRCm38)	V46E	probably damaging	Het
Pum1	A	G	4: 130,728,287 (GRCm38)		probably null	Het
Rbak	G	T	5: 143,176,552 (GRCm38)	Q19K	possibly damaging	Het
Sema3c	A	G	5: 17,576,961 (GRCm38)	T32A	probably damaging	Het
Shprh	T	C	10: 11,167,873 (GRCm38)	W835R	probably damaging	Het
Sis	T	G	3: 72,911,854 (GRCm38)	K1456N	probably damaging	Het
Slc4a4	A	T	5: 89,179,729 (GRCm38)	N675I	probably benign	Het
Slc8a3	C	A	12: 81,315,627 (GRCm38)	M139I	probably damaging	Het
Sorcs3	T	C	19: 48,802,759 (GRCm38)	F1182S	probably damaging	Het
St8sia2	A	T	7: 73,971,921 (GRCm38)	I96N	probably damaging	Het
Stx19	T	C	16: 62,822,057 (GRCm38)	S79P	probably damaging	Het
Tecta	C	T	9: 42,375,267 (GRCm38)	V698M	probably damaging	Het
Timm22	T	C	11: 76,411,139 (GRCm38)	S150P	probably damaging	Het
Tjp1	C	T	7: 65,313,205 (GRCm38)	D995N	possibly damaging	Het
Tmem135	T	A	7: 89,147,794 (GRCm38)	T365S	probably benign	Het
Tmem174	T	C	13: 98,636,981 (GRCm38)	T114A	probably benign	Het
Trcg1	A	G	9: 57,241,330 (GRCm38)	I62V	possibly damaging	Het
Tulp1	A	T	17: 28,356,031 (GRCm38)	*487K	probably null	Het
Unc79	T	A	12: 103,131,646 (GRCm38)	V1826E	possibly damaging	Het
Vmn2r13	A	T	5: 109,174,116 (GRCm38)	N238K	probably damaging	Het
Vmn2r22	T	A	6: 123,637,738 (GRCm38)	N298Y	probably damaging	Het
Vmn2r23	T	A	6: 123,712,902 (GRCm38)	F246I	probably damaging	Het
Vps9d1	A	C	8: 123,248,639 (GRCm38)	V194G	probably damaging	Het
Zfp260	A	T	7: 30,104,810 (GRCm38)	H45L	possibly damaging	Het
Zfp846	A	T	9: 20,593,720 (GRCm38)	H292L	possibly damaging	Het

Other mutations in Tnc

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00229:Tnc	APN	4	64,016,824 (GRCm38)	splice site	probably benign
IGL00531:Tnc	APN	4	63,971,153 (GRCm38)	splice site	probably benign
IGL00674:Tnc	APN	4	63,965,607 (GRCm38)	missense	probably damaging	1.00
IGL01015:Tnc	APN	4	64,017,334 (GRCm38)	missense	probably benign	0.19
IGL01090:Tnc	APN	4	64,000,080 (GRCm38)	missense	probably damaging	1.00
IGL01310:Tnc	APN	4	64,013,077 (GRCm38)	missense	probably benign	0.03
IGL01331:Tnc	APN	4	63,982,875 (GRCm38)	missense	probably damaging	0.99
IGL01393:Tnc	APN	4	64,014,054 (GRCm38)	splice site	probably benign
IGL01411:Tnc	APN	4	64,000,722 (GRCm38)	missense	probably damaging	0.96
IGL01472:Tnc	APN	4	64,006,419 (GRCm38)	missense	probably benign	0.00
IGL01552:Tnc	APN	4	63,970,408 (GRCm38)	missense	probably damaging	1.00
IGL01661:Tnc	APN	4	63,970,307 (GRCm38)	splice site	probably benign
IGL01669:Tnc	APN	4	64,000,701 (GRCm38)	missense	probably damaging	1.00
IGL01912:Tnc	APN	4	64,008,740 (GRCm38)	missense	probably damaging	1.00
IGL02028:Tnc	APN	4	63,966,672 (GRCm38)	splice site	probably benign
IGL02100:Tnc	APN	4	64,000,161 (GRCm38)	missense	possibly damaging	0.84
IGL02549:Tnc	APN	4	64,015,072 (GRCm38)	missense	probably damaging	1.00
IGL02642:Tnc	APN	4	63,965,579 (GRCm38)	splice site	probably benign
IGL02712:Tnc	APN	4	63,975,256 (GRCm38)	missense	probably damaging	1.00
IGL02876:Tnc	APN	4	64,015,101 (GRCm38)	missense	possibly damaging	0.56
IGL02886:Tnc	APN	4	64,000,107 (GRCm38)	missense	probably damaging	0.96
IGL02972:Tnc	APN	4	63,976,478 (GRCm38)	missense	probably benign	0.11
IGL03073:Tnc	APN	4	63,971,224 (GRCm38)	missense	possibly damaging	0.58
IGL03116:Tnc	APN	4	64,014,033 (GRCm38)	missense	probably damaging	1.00
IGL03181:Tnc	APN	4	63,967,306 (GRCm38)	missense	possibly damaging	0.95
IGL03358:Tnc	APN	4	64,017,615 (GRCm38)	nonsense	probably null
tancredo	UTSW	4	63,993,297 (GRCm38)	nonsense	probably null
BB009:Tnc	UTSW	4	64,008,620 (GRCm38)	missense	probably benign
BB019:Tnc	UTSW	4	64,008,620 (GRCm38)	missense	probably benign
P0020:Tnc	UTSW	4	64,008,857 (GRCm38)	missense	possibly damaging	0.63
PIT4377001:Tnc	UTSW	4	64,017,736 (GRCm38)	missense	probably damaging	1.00
PIT4403001:Tnc	UTSW	4	63,964,667 (GRCm38)	missense	probably damaging	1.00
PIT4468001:Tnc	UTSW	4	63,964,667 (GRCm38)	missense	probably damaging	1.00
R0243:Tnc	UTSW	4	63,970,420 (GRCm38)	missense	probably damaging	0.98
R0362:Tnc	UTSW	4	64,017,442 (GRCm38)	missense	probably damaging	1.00
R0410:Tnc	UTSW	4	64,007,694 (GRCm38)	missense	probably benign	0.00
R0420:Tnc	UTSW	4	64,000,159 (GRCm38)	missense	probably benign	0.00
R0540:Tnc	UTSW	4	64,020,455 (GRCm38)	missense	probably damaging	1.00
R0650:Tnc	UTSW	4	64,008,734 (GRCm38)	missense	probably benign	0.00
R1019:Tnc	UTSW	4	63,962,082 (GRCm38)	missense	probably damaging	1.00
R1102:Tnc	UTSW	4	64,020,468 (GRCm38)	missense	probably benign	0.05
R1126:Tnc	UTSW	4	64,018,120 (GRCm38)	missense	probably damaging	0.99
R1141:Tnc	UTSW	4	64,013,994 (GRCm38)	missense	probably damaging	1.00
R1142:Tnc	UTSW	4	64,013,994 (GRCm38)	missense	probably damaging	1.00
R1307:Tnc	UTSW	4	64,008,859 (GRCm38)	missense	probably damaging	0.98
R1322:Tnc	UTSW	4	64,013,994 (GRCm38)	missense	probably damaging	1.00
R1414:Tnc	UTSW	4	63,965,695 (GRCm38)	splice site	probably benign
R1470:Tnc	UTSW	4	63,966,574 (GRCm38)	missense	probably damaging	1.00
R1470:Tnc	UTSW	4	63,966,574 (GRCm38)	missense	probably damaging	1.00
R1499:Tnc	UTSW	4	63,964,754 (GRCm38)	missense	probably benign	0.15
R1506:Tnc	UTSW	4	64,007,684 (GRCm38)	missense	possibly damaging	0.90
R1597:Tnc	UTSW	4	64,006,384 (GRCm38)	missense	probably benign
R1750:Tnc	UTSW	4	63,972,735 (GRCm38)	missense	probably damaging	1.00
R1765:Tnc	UTSW	4	64,013,994 (GRCm38)	missense	probably damaging	1.00
R1783:Tnc	UTSW	4	64,018,096 (GRCm38)	missense	probably damaging	0.98
R1808:Tnc	UTSW	4	63,999,931 (GRCm38)	missense	probably damaging	1.00
R1903:Tnc	UTSW	4	64,000,062 (GRCm38)	missense	probably benign	0.00
R1932:Tnc	UTSW	4	63,993,025 (GRCm38)	critical splice donor site	probably null
R1941:Tnc	UTSW	4	64,014,964 (GRCm38)	missense	probably damaging	1.00
R1983:Tnc	UTSW	4	63,984,630 (GRCm38)	missense	possibly damaging	0.95
R2024:Tnc	UTSW	4	63,964,621 (GRCm38)	missense	probably damaging	1.00
R2075:Tnc	UTSW	4	63,995,666 (GRCm38)	missense	possibly damaging	0.94
R2327:Tnc	UTSW	4	63,975,238 (GRCm38)	missense	possibly damaging	0.78
R2444:Tnc	UTSW	4	64,014,963 (GRCm38)	missense	probably damaging	1.00
R2982:Tnc	UTSW	4	64,020,519 (GRCm38)	missense	possibly damaging	0.81
R3874:Tnc	UTSW	4	64,008,710 (GRCm38)	missense	probably damaging	1.00
R4110:Tnc	UTSW	4	64,014,951 (GRCm38)	missense	probably damaging	1.00
R4360:Tnc	UTSW	4	64,016,924 (GRCm38)	missense	probably benign	0.35
R4371:Tnc	UTSW	4	63,970,351 (GRCm38)	missense	probably damaging	1.00
R4434:Tnc	UTSW	4	64,007,829 (GRCm38)	missense	possibly damaging	0.91
R4438:Tnc	UTSW	4	64,007,829 (GRCm38)	missense	possibly damaging	0.91
R4570:Tnc	UTSW	4	63,995,672 (GRCm38)	missense	probably damaging	0.99
R4595:Tnc	UTSW	4	63,995,745 (GRCm38)	missense	probably damaging	1.00
R4749:Tnc	UTSW	4	63,995,639 (GRCm38)	missense	possibly damaging	0.56
R4756:Tnc	UTSW	4	63,967,343 (GRCm38)	missense	probably damaging	0.99
R4824:Tnc	UTSW	4	64,017,620 (GRCm38)	nonsense	probably null
R4957:Tnc	UTSW	4	63,976,556 (GRCm38)	missense	probably damaging	1.00
R4977:Tnc	UTSW	4	64,006,248 (GRCm38)	missense	possibly damaging	0.82
R5001:Tnc	UTSW	4	64,000,062 (GRCm38)	missense	probably benign	0.16
R5001:Tnc	UTSW	4	63,984,489 (GRCm38)	missense	probably damaging	1.00
R5015:Tnc	UTSW	4	64,006,502 (GRCm38)	missense	probably damaging	1.00
R5049:Tnc	UTSW	4	64,017,986 (GRCm38)	missense	probably damaging	1.00
R5066:Tnc	UTSW	4	63,975,229 (GRCm38)	missense	probably damaging	0.96
R5073:Tnc	UTSW	4	64,020,411 (GRCm38)	missense	probably damaging	1.00
R5116:Tnc	UTSW	4	63,967,215 (GRCm38)	critical splice donor site	probably null
R5195:Tnc	UTSW	4	63,967,252 (GRCm38)	missense	probably damaging	1.00
R5200:Tnc	UTSW	4	63,971,278 (GRCm38)	missense	probably damaging	1.00
R5221:Tnc	UTSW	4	63,993,297 (GRCm38)	nonsense	probably null
R5237:Tnc	UTSW	4	63,962,096 (GRCm38)	missense	probably damaging	1.00
R5265:Tnc	UTSW	4	63,993,206 (GRCm38)	missense	probably benign	0.00
R5275:Tnc	UTSW	4	63,964,730 (GRCm38)	nonsense	probably null
R5346:Tnc	UTSW	4	64,008,655 (GRCm38)	missense	probably benign
R5409:Tnc	UTSW	4	64,007,417 (GRCm38)	missense	probably damaging	1.00
R5409:Tnc	UTSW	4	63,966,536 (GRCm38)	missense	probably damaging	1.00
R5469:Tnc	UTSW	4	64,013,925 (GRCm38)	splice site	probably null
R5518:Tnc	UTSW	4	64,017,679 (GRCm38)	missense	probably damaging	1.00
R5560:Tnc	UTSW	4	64,008,709 (GRCm38)	missense	probably damaging	1.00
R5588:Tnc	UTSW	4	64,006,422 (GRCm38)	missense	possibly damaging	0.57
R5686:Tnc	UTSW	4	64,007,730 (GRCm38)	splice site	probably null
R5686:Tnc	UTSW	4	64,008,795 (GRCm38)	missense	possibly damaging	0.78
R5837:Tnc	UTSW	4	64,013,214 (GRCm38)	missense	probably damaging	1.00
R5976:Tnc	UTSW	4	64,018,166 (GRCm38)	missense	probably benign	0.17
R6156:Tnc	UTSW	4	63,970,352 (GRCm38)	missense	probably damaging	1.00
R6182:Tnc	UTSW	4	64,008,796 (GRCm38)	missense	probably damaging	0.99
R6360:Tnc	UTSW	4	64,000,733 (GRCm38)	missense	probably damaging	1.00
R6778:Tnc	UTSW	4	63,995,598 (GRCm38)	missense	probably benign	0.12
R6798:Tnc	UTSW	4	63,965,604 (GRCm38)	missense	probably benign	0.02
R6799:Tnc	UTSW	4	63,965,604 (GRCm38)	missense	probably benign	0.02
R6943:Tnc	UTSW	4	63,982,745 (GRCm38)	missense	probably damaging	0.97
R7027:Tnc	UTSW	4	63,984,589 (GRCm38)	missense	probably benign	0.02
R7183:Tnc	UTSW	4	64,013,128 (GRCm38)	missense	probably damaging	1.00
R7204:Tnc	UTSW	4	63,971,155 (GRCm38)	splice site	probably null
R7317:Tnc	UTSW	4	63,972,722 (GRCm38)	missense	probably damaging	0.99
R7323:Tnc	UTSW	4	63,971,232 (GRCm38)	missense	probably damaging	0.96
R7327:Tnc	UTSW	4	63,964,762 (GRCm38)	splice site	probably null
R7382:Tnc	UTSW	4	64,014,043 (GRCm38)	nonsense	probably null
R7399:Tnc	UTSW	4	64,020,657 (GRCm38)	start gained	probably benign
R7479:Tnc	UTSW	4	64,017,628 (GRCm38)	missense	possibly damaging	0.95
R7585:Tnc	UTSW	4	64,020,411 (GRCm38)	missense	probably damaging	1.00
R7932:Tnc	UTSW	4	64,008,620 (GRCm38)	missense	probably benign
R7947:Tnc	UTSW	4	64,017,343 (GRCm38)	missense	probably damaging	1.00
R7974:Tnc	UTSW	4	64,000,724 (GRCm38)	missense	possibly damaging	0.84
R7991:Tnc	UTSW	4	64,008,746 (GRCm38)	missense	probably benign	0.42
R8004:Tnc	UTSW	4	63,984,657 (GRCm38)	missense	probably benign	0.04
R8080:Tnc	UTSW	4	63,976,469 (GRCm38)	missense	possibly damaging	0.52
R8109:Tnc	UTSW	4	64,008,763 (GRCm38)	missense	probably benign	0.11
R8145:Tnc	UTSW	4	64,017,479 (GRCm38)	missense	probably benign
R8340:Tnc	UTSW	4	64,007,799 (GRCm38)	missense	probably damaging	1.00
R8360:Tnc	UTSW	4	63,967,274 (GRCm38)	missense	probably benign	0.00
R8671:Tnc	UTSW	4	64,017,446 (GRCm38)	missense	probably damaging	1.00
R8691:Tnc	UTSW	4	63,962,076 (GRCm38)	missense	probably damaging	1.00
R8759:Tnc	UTSW	4	64,006,264 (GRCm38)	missense	possibly damaging	0.86
R8864:Tnc	UTSW	4	63,993,059 (GRCm38)	missense	probably damaging	0.98
R8927:Tnc	UTSW	4	64,007,358 (GRCm38)	missense	probably damaging	1.00
R8928:Tnc	UTSW	4	64,007,358 (GRCm38)	missense	probably damaging	1.00
R8949:Tnc	UTSW	4	64,008,850 (GRCm38)	missense	probably damaging	1.00
R8956:Tnc	UTSW	4	64,000,733 (GRCm38)	missense	probably damaging	1.00
R9016:Tnc	UTSW	4	64,017,094 (GRCm38)	missense	probably benign	0.23
R9049:Tnc	UTSW	4	64,000,010 (GRCm38)	missense	possibly damaging	0.83
R9097:Tnc	UTSW	4	63,970,385 (GRCm38)	missense	possibly damaging	0.62
R9114:Tnc	UTSW	4	63,972,736 (GRCm38)	missense	probably benign	0.03
R9151:Tnc	UTSW	4	64,020,449 (GRCm38)	missense	possibly damaging	0.46
R9488:Tnc	UTSW	4	63,995,705 (GRCm38)	missense	probably damaging	0.99
R9537:Tnc	UTSW	4	63,966,584 (GRCm38)	missense	probably damaging	0.99
R9666:Tnc	UTSW	4	64,007,808 (GRCm38)	missense	probably damaging	1.00
R9700:Tnc	UTSW	4	64,014,949 (GRCm38)	missense	probably damaging	0.99
R9703:Tnc	UTSW	4	63,971,175 (GRCm38)	missense	probably benign	0.00
R9771:Tnc	UTSW	4	64,007,363 (GRCm38)	missense	probably damaging	1.00
S24628:Tnc	UTSW	4	64,018,012 (GRCm38)	missense	probably damaging	1.00
Z1177:Tnc	UTSW	4	64,007,426 (GRCm38)	nonsense	probably null
Z1177:Tnc	UTSW	4	63,960,544 (GRCm38)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- AAATAGGGACATATGCTCCTGTC -3'
(R):5'- ATCAGTTACCAAGACAGGGGC -3'

Sequencing Primer
(F):5'- GACATATGCTCCTGTCCTTTCGAGG -3'
(R):5'- TTACCAAGACAGGGGCTTCGG -3'

Posted On

2018-05-24