Mutagenetix > Incidental Mutation

Incidental Mutation 'R6424:Tars3'

518262

Institutional Source

Beutler Lab

Gene Symbol

Tars3

Ensembl Gene

ENSMUSG00000030515

Gene Name

threonyl-tRNA synthetase 3

Synonyms

A530046H20Rik, Tarsl2

MMRRC Submission

044387-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.185) question?

Stock #

R6424 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

65294646-65341839 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 65305487 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Glutamic Acid at position 237 (G237E)

Ref Sequence

ENSEMBL: ENSMUSP00000032728 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032728]

AlphaFold

Q8BLY2

Predicted Effect

probably damaging
Transcript: ENSMUST00000032728
AA Change: G237E

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000032728
Gene: ENSMUSG00000030515
AA Change: G237E

Domain	Start	End	E-Value	Type
low complexity region	2	18	N/A	INTRINSIC
coiled coil region	44	68	N/A	INTRINSIC
Pfam:TGS	151	210	8.8e-14	PFAM
tRNA_SAD	316	365	1.26e-16	SMART
Pfam:tRNA-synt_2b	464	675	2.2e-35	PFAM
Pfam:HGTP_anticodon	687	778	1.1e-20	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126941

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000206323

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 97.8%
20x: 93.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 21 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	T	C	11: 9,460,542 (GRCm39)	V4184A	probably benign	Het
Acvr2b	T	A	9: 119,231,645 (GRCm39)	W12R	probably benign	Het
Arap2	T	C	5: 62,840,707 (GRCm39)	K720E	probably damaging	Het
Cr1l	A	C	1: 194,800,123 (GRCm39)	F184V	probably damaging	Het
Haus8	C	T	8: 71,704,080 (GRCm39)	W359*	probably null	Het
Insm2	A	T	12: 55,646,867 (GRCm39)	I204F	probably damaging	Het
Katnb1	T	A	8: 95,820,144 (GRCm39)	I97N	probably damaging	Het
Kif1b	T	C	4: 149,277,053 (GRCm39)	M1337V	probably benign	Het
Map2	A	T	1: 66,453,946 (GRCm39)	K945N	possibly damaging	Het
Meltf	T	A	16: 31,699,080 (GRCm39)	C63*	probably null	Het
Nbas	T	C	12: 13,465,734 (GRCm39)		probably null	Het
Or5p1	G	A	7: 107,916,412 (GRCm39)	V104I	probably benign	Het
Raf1	T	C	6: 115,596,542 (GRCm39)	E594G	probably benign	Het
Rsrc1	C	T	3: 66,901,982 (GRCm39)	P44L	unknown	Het
Scgb2b19	A	C	7: 32,978,022 (GRCm39)	S92A	possibly damaging	Het
Serpinb3c	T	A	1: 107,199,359 (GRCm39)	*387Y	probably null	Het
Shpk	A	T	11: 73,104,318 (GRCm39)	I156F	possibly damaging	Het
Smarcd1	T	C	15: 99,602,248 (GRCm39)	F128L	probably damaging	Het
Thap1	CAGCATCTGCTCGGAGCA	CAGCA	8: 26,650,884 (GRCm39)		probably null	Het
Ttn	T	C	2: 76,719,848 (GRCm39)		probably benign	Het
Vmn1r223	T	A	13: 23,434,345 (GRCm39)	I313N	probably damaging	Het

Other mutations in Tars3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00092:Tars3	APN	7	65,302,007 (GRCm39)	critical splice acceptor site	probably null
IGL00470:Tars3	APN	7	65,338,656 (GRCm39)	missense	probably benign	0.03
IGL00594:Tars3	APN	7	65,325,880 (GRCm39)	critical splice donor site	probably null
IGL01352:Tars3	APN	7	65,308,658 (GRCm39)	missense	possibly damaging	0.80
IGL01519:Tars3	APN	7	65,313,634 (GRCm39)	missense	probably damaging	1.00
IGL01726:Tars3	APN	7	65,332,566 (GRCm39)	missense	possibly damaging	0.46
IGL02370:Tars3	APN	7	65,310,913 (GRCm39)	missense	probably benign	0.17
IGL02729:Tars3	APN	7	65,332,567 (GRCm39)	missense	probably damaging	0.97
IGL03234:Tars3	APN	7	65,302,026 (GRCm39)	missense	probably benign	0.06
gary	UTSW	7	65,338,700 (GRCm39)	critical splice donor site	probably null
R8254_tarsl2_650	UTSW	7	65,325,809 (GRCm39)	missense	probably benign
smart_money	UTSW	7	65,327,890 (GRCm39)	missense	probably damaging	1.00
R0127:Tars3	UTSW	7	65,314,717 (GRCm39)	missense	probably benign	0.19
R0153:Tars3	UTSW	7	65,333,829 (GRCm39)	missense	probably damaging	1.00
R0605:Tars3	UTSW	7	65,327,819 (GRCm39)	missense	probably damaging	1.00
R1070:Tars3	UTSW	7	65,305,444 (GRCm39)	missense	probably damaging	1.00
R1450:Tars3	UTSW	7	65,297,244 (GRCm39)	missense	probably benign	0.01
R1467:Tars3	UTSW	7	65,305,444 (GRCm39)	missense	probably damaging	1.00
R1467:Tars3	UTSW	7	65,305,444 (GRCm39)	missense	probably damaging	1.00
R2142:Tars3	UTSW	7	65,308,645 (GRCm39)	missense	probably benign
R2143:Tars3	UTSW	7	65,305,539 (GRCm39)	missense	possibly damaging	0.57
R2144:Tars3	UTSW	7	65,305,539 (GRCm39)	missense	possibly damaging	0.57
R2145:Tars3	UTSW	7	65,305,539 (GRCm39)	missense	possibly damaging	0.57
R2208:Tars3	UTSW	7	65,332,596 (GRCm39)	missense	probably damaging	1.00
R3713:Tars3	UTSW	7	65,338,700 (GRCm39)	critical splice donor site	probably null
R3715:Tars3	UTSW	7	65,338,700 (GRCm39)	critical splice donor site	probably null
R3914:Tars3	UTSW	7	65,333,556 (GRCm39)	missense	probably benign	0.05
R3929:Tars3	UTSW	7	65,333,791 (GRCm39)	splice site	probably null
R4008:Tars3	UTSW	7	65,327,876 (GRCm39)	missense	probably damaging	1.00
R4064:Tars3	UTSW	7	65,302,018 (GRCm39)	missense	possibly damaging	0.90
R4367:Tars3	UTSW	7	65,332,567 (GRCm39)	missense	probably damaging	0.97
R4652:Tars3	UTSW	7	65,339,717 (GRCm39)	missense	probably damaging	1.00
R4825:Tars3	UTSW	7	65,297,302 (GRCm39)	missense	probably benign	0.38
R4901:Tars3	UTSW	7	65,341,042 (GRCm39)	missense	probably benign	0.05
R4999:Tars3	UTSW	7	65,308,683 (GRCm39)	missense	probably damaging	0.99
R5423:Tars3	UTSW	7	65,333,567 (GRCm39)	missense	probably benign	0.00
R5756:Tars3	UTSW	7	65,325,724 (GRCm39)	missense	probably benign	0.22
R5772:Tars3	UTSW	7	65,333,873 (GRCm39)	missense	probably damaging	1.00
R6160:Tars3	UTSW	7	65,332,527 (GRCm39)	missense	probably benign	0.32
R6230:Tars3	UTSW	7	65,336,184 (GRCm39)	splice site	probably null
R6615:Tars3	UTSW	7	65,327,890 (GRCm39)	missense	probably damaging	1.00
R6792:Tars3	UTSW	7	65,312,051 (GRCm39)	missense	probably damaging	1.00
R7350:Tars3	UTSW	7	65,308,672 (GRCm39)	missense	probably damaging	1.00
R7549:Tars3	UTSW	7	65,297,341 (GRCm39)	missense	probably damaging	0.96
R7592:Tars3	UTSW	7	65,308,619 (GRCm39)	missense	probably benign	0.01
R7634:Tars3	UTSW	7	65,325,760 (GRCm39)	missense	probably damaging	0.99
R7710:Tars3	UTSW	7	65,314,717 (GRCm39)	missense	probably benign	0.19
R7808:Tars3	UTSW	7	65,302,009 (GRCm39)	missense	probably benign	0.01
R7875:Tars3	UTSW	7	65,327,899 (GRCm39)	missense	probably benign	0.05
R8254:Tars3	UTSW	7	65,325,809 (GRCm39)	missense	probably benign
R8793:Tars3	UTSW	7	65,294,673 (GRCm39)	start gained	probably benign
R9162:Tars3	UTSW	7	65,332,518 (GRCm39)	missense	probably benign	0.01
R9200:Tars3	UTSW	7	65,302,013 (GRCm39)	missense	probably benign
R9461:Tars3	UTSW	7	65,339,719 (GRCm39)	missense	possibly damaging	0.68
R9533:Tars3	UTSW	7	65,333,808 (GRCm39)	critical splice acceptor site	probably null
Z1177:Tars3	UTSW	7	65,302,012 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AAGTTTGCAGGGGAGCATGC -3'
(R):5'- CCAGTTTTCACAAATGGCTAACC -3'

Sequencing Primer
(F):5'- CACATAGGTGTTGGGGACTCTCTAC -3'
(R):5'- TGGCTAACCACTTGACAATCAC -3'

Posted On

2018-05-24