Mutagenetix > Incidental Mutation

Incidental Mutation 'R6428:Vmn2r26'

518391

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r26

Ensembl Gene

ENSMUSG00000096630

Gene Name

vomeronasal 2, receptor 26

Synonyms

V2r1b

MMRRC Submission

044421-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.088) question?

Stock #

R6428 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

124024758-124062035 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 124026080 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 150 (I150V)

Ref Sequence

ENSEMBL: ENSMUSP00000032238 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032238]

AlphaFold

Q6TAC4

Predicted Effect

probably benign
Transcript: ENSMUST00000032238
AA Change: I150V

PolyPhen 2 Score 0.172 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000032238
Gene: ENSMUSG00000096630
AA Change: I150V

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	82	471	1.5e-31	PFAM
Pfam:NCD3G	519	572	4.6e-25	PFAM
Pfam:7tm_3	603	840	1.5e-55	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.6%
20x: 92.3%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit abnormal vomeronasal sensory neuron physiology and avnosmia. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 22 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abat	C	T	16: 8,602,436 (GRCm38)		probably benign	Het
Cts6	T	G	13: 61,196,423 (GRCm38)	N272H	probably damaging	Het
Daam2	C	T	17: 49,469,376 (GRCm38)	E828K	probably damaging	Het
Dhx9	C	T	1: 153,456,578 (GRCm38)		probably benign	Het
Glb1l3	T	C	9: 26,859,452 (GRCm38)	N106S	probably damaging	Het
Gm1141	CATCTTTACCATCATGTCAAGTATCTTTACCATCATGTCAAGTATCTTTACC	CATCTTTACCATCATGTCAAGTATCTTTACC	X: 71,939,520 (GRCm38)		probably benign	Homo
Hdlbp	T	C	1: 93,431,445 (GRCm38)	D175G	possibly damaging	Het
Hectd4	A	G	5: 121,350,445 (GRCm38)	K3600E	possibly damaging	Het
Hspa13	A	T	16: 75,757,986 (GRCm38)	L404Q	probably damaging	Het
Iws1	A	G	18: 32,086,290 (GRCm38)	D475G	probably damaging	Het
Klhdc10	T	C	6: 30,439,856 (GRCm38)	S13P	probably damaging	Het
Lepr	C	T	4: 101,780,098 (GRCm38)	T728I	probably damaging	Het
Ncapd3	A	G	9: 27,052,664 (GRCm38)		probably null	Het
Phb2	A	G	6: 124,715,991 (GRCm38)	D276G	probably benign	Het
Prrc2b	A	G	2: 32,226,496 (GRCm38)		probably null	Het
Rasgrf2	T	C	13: 91,987,981 (GRCm38)	K604R	probably damaging	Het
Rp1l1	T	A	14: 64,032,389 (GRCm38)	M1808K	possibly damaging	Het
Setd3	T	C	12: 108,113,338 (GRCm38)	D302G	probably damaging	Het
Slc38a10	T	A	11: 120,105,472 (GRCm38)	Q933L	probably benign	Het
Slco1a1	A	G	6: 141,925,690 (GRCm38)	L250S	probably damaging	Het
Vmn2r106	C	T	17: 20,268,463 (GRCm38)	C558Y	probably damaging	Het
Zfp318	C	T	17: 46,399,336 (GRCm38)	R662C	probably damaging	Het

Other mutations in Vmn2r26

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01070:Vmn2r26	APN	6	124,061,607 (GRCm38)	missense	probably benign	0.00
IGL01370:Vmn2r26	APN	6	124,061,756 (GRCm38)	missense	probably benign	0.08
IGL01603:Vmn2r26	APN	6	124,053,874 (GRCm38)	missense	probably damaging	1.00
IGL01651:Vmn2r26	APN	6	124,050,673 (GRCm38)	missense	probably benign	0.01
IGL02282:Vmn2r26	APN	6	124,061,625 (GRCm38)	missense	probably damaging	1.00
IGL02425:Vmn2r26	APN	6	124,061,818 (GRCm38)	missense	probably damaging	1.00
IGL02551:Vmn2r26	APN	6	124,026,141 (GRCm38)	missense	probably benign	0.11
IGL02690:Vmn2r26	APN	6	124,026,132 (GRCm38)	missense	probably benign	0.14
IGL03002:Vmn2r26	APN	6	124,039,795 (GRCm38)	missense	possibly damaging	0.78
IGL03270:Vmn2r26	APN	6	124,050,819 (GRCm38)	missense	probably benign	0.16
R0032:Vmn2r26	UTSW	6	124,039,899 (GRCm38)	missense	possibly damaging	0.72
R0052:Vmn2r26	UTSW	6	124,062,033 (GRCm38)	makesense	probably null
R0083:Vmn2r26	UTSW	6	124,053,981 (GRCm38)	splice site	probably null
R0682:Vmn2r26	UTSW	6	124,061,170 (GRCm38)	missense	probably damaging	0.97
R1061:Vmn2r26	UTSW	6	124,061,644 (GRCm38)	missense	probably benign	0.12
R1077:Vmn2r26	UTSW	6	124,053,913 (GRCm38)	missense	probably benign	0.00
R1263:Vmn2r26	UTSW	6	124,050,708 (GRCm38)	missense	probably benign
R1579:Vmn2r26	UTSW	6	124,039,747 (GRCm38)	missense	probably benign	0.00
R1741:Vmn2r26	UTSW	6	124,061,472 (GRCm38)	missense	probably damaging	1.00
R1834:Vmn2r26	UTSW	6	124,061,410 (GRCm38)	missense	possibly damaging	0.54
R1838:Vmn2r26	UTSW	6	124,024,771 (GRCm38)	missense	probably benign
R1956:Vmn2r26	UTSW	6	124,053,887 (GRCm38)	missense	probably damaging	1.00
R1996:Vmn2r26	UTSW	6	124,061,185 (GRCm38)	missense	probably damaging	1.00
R2140:Vmn2r26	UTSW	6	124,061,237 (GRCm38)	missense	probably benign	0.01
R2327:Vmn2r26	UTSW	6	124,039,749 (GRCm38)	missense	probably benign	0.07
R2417:Vmn2r26	UTSW	6	124,061,350 (GRCm38)	missense	probably damaging	1.00
R3930:Vmn2r26	UTSW	6	124,025,979 (GRCm38)	missense	probably benign
R4490:Vmn2r26	UTSW	6	124,050,738 (GRCm38)	missense	possibly damaging	0.47
R4629:Vmn2r26	UTSW	6	124,061,191 (GRCm38)	missense	possibly damaging	0.50
R4655:Vmn2r26	UTSW	6	124,061,416 (GRCm38)	missense	probably damaging	1.00
R4709:Vmn2r26	UTSW	6	124,053,965 (GRCm38)	missense	probably damaging	1.00
R4992:Vmn2r26	UTSW	6	124,026,111 (GRCm38)	missense	probably benign	0.00
R5297:Vmn2r26	UTSW	6	124,061,873 (GRCm38)	missense	probably damaging	1.00
R5482:Vmn2r26	UTSW	6	124,061,326 (GRCm38)	missense	possibly damaging	0.88
R5517:Vmn2r26	UTSW	6	124,050,717 (GRCm38)	missense	probably damaging	1.00
R5737:Vmn2r26	UTSW	6	124,039,449 (GRCm38)	missense	probably benign	0.00
R5739:Vmn2r26	UTSW	6	124,025,966 (GRCm38)	missense	probably benign	0.00
R5873:Vmn2r26	UTSW	6	124,061,674 (GRCm38)	missense	probably benign	0.01
R5907:Vmn2r26	UTSW	6	124,039,871 (GRCm38)	missense	probably benign	0.00
R6086:Vmn2r26	UTSW	6	124,039,560 (GRCm38)	missense	possibly damaging	0.48
R6134:Vmn2r26	UTSW	6	124,061,485 (GRCm38)	missense	probably damaging	0.97
R6391:Vmn2r26	UTSW	6	124,061,389 (GRCm38)	missense	probably damaging	1.00
R6637:Vmn2r26	UTSW	6	124,061,691 (GRCm38)	missense	probably damaging	1.00
R6927:Vmn2r26	UTSW	6	124,039,098 (GRCm38)	missense	possibly damaging	0.93
R6953:Vmn2r26	UTSW	6	124,039,782 (GRCm38)	missense	probably benign	0.00
R7173:Vmn2r26	UTSW	6	124,061,296 (GRCm38)	missense	probably benign	0.16
R7206:Vmn2r26	UTSW	6	124,039,768 (GRCm38)	missense	probably benign	0.17
R7208:Vmn2r26	UTSW	6	124,061,989 (GRCm38)	missense	probably damaging	1.00
R7283:Vmn2r26	UTSW	6	124,025,955 (GRCm38)	missense	probably damaging	0.97
R7506:Vmn2r26	UTSW	6	124,039,741 (GRCm38)	missense	probably benign	0.00
R7672:Vmn2r26	UTSW	6	124,039,647 (GRCm38)	missense	probably benign	0.25
R7674:Vmn2r26	UTSW	6	124,039,362 (GRCm38)	missense	probably benign
R7696:Vmn2r26	UTSW	6	124,061,535 (GRCm38)	missense	possibly damaging	0.94
R7716:Vmn2r26	UTSW	6	124,061,745 (GRCm38)	missense	probably damaging	1.00
R7831:Vmn2r26	UTSW	6	124,039,799 (GRCm38)	nonsense	probably null
R8063:Vmn2r26	UTSW	6	124,024,955 (GRCm38)	missense	probably benign	0.00
R8331:Vmn2r26	UTSW	6	124,061,928 (GRCm38)	missense	probably benign	0.22
R8352:Vmn2r26	UTSW	6	124,039,618 (GRCm38)	missense	probably benign	0.09
R8445:Vmn2r26	UTSW	6	124,026,036 (GRCm38)	missense	probably damaging	0.97
R8452:Vmn2r26	UTSW	6	124,039,618 (GRCm38)	missense	probably benign	0.09
R8681:Vmn2r26	UTSW	6	124,024,918 (GRCm38)	missense	probably benign	0.00
R8914:Vmn2r26	UTSW	6	124,062,024 (GRCm38)	missense	probably benign
R9333:Vmn2r26	UTSW	6	124,026,050 (GRCm38)	missense	probably benign	0.13
R9351:Vmn2r26	UTSW	6	124,039,374 (GRCm38)	missense	probably benign
R9436:Vmn2r26	UTSW	6	124,025,867 (GRCm38)	missense	probably damaging	1.00
R9515:Vmn2r26	UTSW	6	124,061,178 (GRCm38)	missense	probably damaging	1.00
RF010:Vmn2r26	UTSW	6	124,039,489 (GRCm38)	missense	possibly damaging	0.90

Predicted Primers

PCR Primer
(F):5'- GCTCTTTATATGCCTTAGATTGCTG -3'
(R):5'- ATCGTGGGCTACATAGGAAGTTC -3'

Sequencing Primer
(F):5'- TCCAAAATTTGAGAGGGAGCACTG -3'
(R):5'- ATGAAAGTTGGTATAGTGAGTTGGC -3'

Posted On

2018-05-24