Incidental Mutation 'R6434:Tril'
ID518692
Institutional Source Beutler Lab
Gene Symbol Tril
Ensembl Gene ENSMUSG00000043496
Gene NameTLR4 interactor with leucine-rich repeats
Synonyms
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.116) question?
Stock #R6434 (G1)
Quality Score225.009
Status Validated
Chromosome6
Chromosomal Location53815468-53820830 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) G to C at 53818508 bp
ZygosityHeterozygous
Amino Acid Change Aspartic acid to Glutamic Acid at position 576 (D576E)
Ref Sequence ENSEMBL: ENSMUSP00000116056 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000127748]
Predicted Effect noncoding transcript
Transcript: ENSMUST00000104970
Predicted Effect probably damaging
Transcript: ENSMUST00000127748
AA Change: D576E

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000116056
Gene: ENSMUSG00000043496
AA Change: D576E

DomainStartEndE-ValueType
signal peptide 1 25 N/A INTRINSIC
LRRNT 26 58 1.91e-1 SMART
LRR_TYP 82 105 3.49e-5 SMART
LRR_TYP 106 129 2.91e-2 SMART
LRR 130 153 9.96e-1 SMART
LRR 154 177 2.49e-1 SMART
LRR_TYP 178 201 1.67e-2 SMART
LRR 202 227 1.09e2 SMART
LRR_TYP 228 251 4.47e-3 SMART
LRR_TYP 252 275 1.84e-4 SMART
LRR 276 299 7.05e-1 SMART
LRR_TYP 300 323 1.3e-4 SMART
LRR 325 347 1.12e1 SMART
LRRCT 359 415 1.02e-2 SMART
low complexity region 448 465 N/A INTRINSIC
low complexity region 488 506 N/A INTRINSIC
low complexity region 554 569 N/A INTRINSIC
Blast:FN3 582 667 8e-34 BLAST
transmembrane domain 695 717 N/A INTRINSIC
low complexity region 720 732 N/A INTRINSIC
low complexity region 788 796 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000204633
Predicted Effect noncoding transcript
Transcript: ENSMUST00000204648
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.5%
  • 10x: 97.7%
  • 20x: 92.8%
Validation Efficiency 98% (57/58)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] TRIL is a component of the TLR4 (MIM 603030) complex and is induced in a number of cell types by lipopolysaccharide (LPS) (Carpenter et al., 2009 [PubMed 19710467]).[supplied by OMIM, Apr 2010]
Allele List at MGI
Other mutations in this stock
Total: 57 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Alcam A C 16: 52,288,827 probably null Het
Ankle2 A G 5: 110,253,893 Y807C probably damaging Het
Arhgef26 A C 3: 62,428,914 M625L probably damaging Het
Aspdh G A 7: 44,467,050 A86T probably damaging Het
Atl1 G T 12: 69,959,425 E502* probably null Het
Atp6v1c1 T C 15: 38,677,546 F105S probably damaging Het
Auh C A 13: 52,929,410 G17C probably benign Het
Ccdc130 A G 8: 84,263,001 I63T probably damaging Het
Ccdc81 T C 7: 89,876,144 Y474C probably damaging Het
Ccdc82 T C 9: 13,252,034 probably benign Het
Ccdc96 T G 5: 36,486,363 V571G probably damaging Het
Col6a5 C A 9: 105,937,345 E489D unknown Het
Dchs2 T A 3: 83,269,270 I845N probably damaging Het
Dnajc11 T C 4: 151,979,294 V449A probably damaging Het
Ect2 A T 3: 27,139,119 Y269* probably null Het
Egfr T C 11: 16,869,294 Y275H probably benign Het
Fan1 T A 7: 64,354,381 D779V probably damaging Het
Fancm T C 12: 65,077,168 V200A probably damaging Het
Frem1 A G 4: 82,966,016 I1214T probably benign Het
Gm3415 T A 5: 146,557,942 F138L probably benign Het
Gtse1 C A 15: 85,875,169 T626K probably benign Het
Herc1 T A 9: 66,486,182 H4114Q probably damaging Het
Ifi44 T A 3: 151,749,189 N133I probably benign Het
Ilf3 A G 9: 21,403,151 probably benign Het
Inpp4a A G 1: 37,398,838 T593A probably damaging Het
Iqgap2 A T 13: 95,682,933 W634R possibly damaging Het
Kcnh4 T A 11: 100,750,279 N448I probably damaging Het
Klhl33 G A 14: 50,893,107 A310V probably damaging Het
Klra9 G T 6: 130,179,032 Y253* probably null Het
Lrig2 T C 3: 104,491,547 K222E possibly damaging Het
Manba G A 3: 135,511,973 probably null Het
Mga A T 2: 119,923,938 Q976L probably damaging Het
Mtmr4 A G 11: 87,613,483 D1086G probably damaging Het
Muc20 A G 16: 32,794,806 V67A probably benign Het
Myh3 T A 11: 67,082,367 L97Q probably damaging Het
Nars T C 18: 64,507,801 T195A probably benign Het
Npat A T 9: 53,563,439 I844L possibly damaging Het
Nrxn3 T C 12: 88,795,515 F111L probably benign Het
Obp2b T C 2: 25,738,587 Y118H probably damaging Het
Olfr1134 T A 2: 87,656,214 K236* probably null Het
Olfr862 A T 9: 19,883,845 Y153* probably null Het
Patj A G 4: 98,491,629 D215G probably damaging Het
Pex1 T A 5: 3,630,196 Y979* probably null Het
Sept3 G A 15: 82,279,603 V54I possibly damaging Het
Shc3 A G 13: 51,449,290 Y260H probably damaging Het
Simc1 A G 13: 54,526,664 T942A probably benign Het
Slco1c1 T C 6: 141,547,850 S371P probably damaging Het
Snrnp200 T A 2: 127,238,654 I2029N probably damaging Het
Syne1 A G 10: 5,318,422 V2089A probably benign Het
Syne2 T C 12: 76,041,456 S5016P probably damaging Het
Tubb2b C T 13: 34,127,578 A411T probably damaging Het
Ubr4 T C 4: 139,429,638 Y2325H probably damaging Het
Utp20 A T 10: 88,772,533 C1547* probably null Het
Utrn A T 10: 12,525,427 W98R probably damaging Het
Vps35 C T 8: 85,273,495 D501N possibly damaging Het
Wdr48 T C 9: 119,916,813 S421P possibly damaging Het
Zfp719 C A 7: 43,590,988 H667N probably damaging Het
Other mutations in Tril
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01103:Tril APN 6 53819038 missense probably damaging 1.00
IGL03399:Tril APN 6 53820057 missense probably benign 0.01
R0036:Tril UTSW 6 53818633 missense probably benign
R0099:Tril UTSW 6 53818363 missense probably damaging 0.99
R0448:Tril UTSW 6 53817808 makesense probably null
R0759:Tril UTSW 6 53818027 missense probably damaging 0.98
R1296:Tril UTSW 6 53818027 missense probably damaging 0.98
R1472:Tril UTSW 6 53818027 missense probably damaging 0.98
R1888:Tril UTSW 6 53819592 missense probably damaging 1.00
R1888:Tril UTSW 6 53819592 missense probably damaging 1.00
R2108:Tril UTSW 6 53819083 missense probably damaging 1.00
R2290:Tril UTSW 6 53818027 missense probably damaging 0.98
R2291:Tril UTSW 6 53818027 missense probably damaging 0.98
R2367:Tril UTSW 6 53819166 missense probably damaging 1.00
R4021:Tril UTSW 6 53819019 missense probably damaging 1.00
R4753:Tril UTSW 6 53819713 missense probably damaging 1.00
R4755:Tril UTSW 6 53818464 missense probably damaging 0.99
R4981:Tril UTSW 6 53818920 missense probably benign 0.10
R5468:Tril UTSW 6 53819647 missense probably damaging 1.00
R5653:Tril UTSW 6 53817985 missense probably benign 0.32
R6724:Tril UTSW 6 53819574 missense possibly damaging 0.94
R7401:Tril UTSW 6 53818281 missense possibly damaging 0.54
R7582:Tril UTSW 6 53818936 missense probably benign
Z1088:Tril UTSW 6 53818920 missense probably benign 0.45
Z1177:Tril UTSW 6 53819644 missense possibly damaging 0.90
Predicted Primers PCR Primer
(F):5'- TAGACGAAGCGCTGGAACTTG -3'
(R):5'- AGCGCATTAAGGTTGAGCCG -3'

Sequencing Primer
(F):5'- GAACTTGGGCTGCTGGC -3'
(R):5'- TCCTTGGACCTGCACGAGAAG -3'
Posted On2018-05-24