Mutagenetix > Incidental Mutation

Incidental Mutation 'R6434:Or7e170'

518702

Institutional Source

Beutler Lab

Gene Symbol

Or7e170

Ensembl Gene

ENSMUSG00000063842

Gene Name

olfactory receptor family 7 subfamily E member 170

Synonyms

Olfr862, MOR146-1, GA_x6K02T2PVTD-13624132-13623212

MMRRC Submission

044572-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.054) question?

Stock #

R6434 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19794679-19795969 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 19795141 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 153 (Y153*)

Ref Sequence

ENSEMBL: ENSMUSP00000078603 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073765] [ENSMUST00000079660]

AlphaFold

Q8VFJ1

Predicted Effect

probably null
Transcript: ENSMUST00000073765
AA Change: Y153*

SMART Domains

Protein: ENSMUSP00000073437
Gene: ENSMUSG00000063842
AA Change: Y153*

Domain	Start	End	E-Value	Type
low complexity region	6	17	N/A	INTRINSIC
Pfam:7tm_4	31	306	3.1e-52	PFAM
Pfam:7TM_GPCR_Srsx	35	282	1.3e-7	PFAM
Pfam:7tm_1	41	290	5.7e-24	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000079660
AA Change: Y153*

SMART Domains

Protein: ENSMUSP00000078603
Gene: ENSMUSG00000063842
AA Change: Y153*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.3e-51	PFAM
Pfam:7TM_GPCR_Srsx	35	303	8.3e-8	PFAM
Pfam:7tm_1	41	290	4.5e-22	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.7%
20x: 92.8%

Validation Efficiency

98% (57/58)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Alcam	A	C	16: 52,109,190 (GRCm39)		probably null	Het
Ankle2	A	G	5: 110,401,759 (GRCm39)	Y807C	probably damaging	Het
Arhgef26	A	C	3: 62,336,335 (GRCm39)	M625L	probably damaging	Het
Aspdh	G	A	7: 44,116,474 (GRCm39)	A86T	probably damaging	Het
Atl1	G	T	12: 70,006,199 (GRCm39)	E502*	probably null	Het
Atp6v1c1	T	C	15: 38,677,790 (GRCm39)	F105S	probably damaging	Het
Auh	C	A	13: 53,083,446 (GRCm39)	G17C	probably benign	Het
Ccdc81	T	C	7: 89,525,352 (GRCm39)	Y474C	probably damaging	Het
Ccdc82	T	C	9: 13,251,659 (GRCm39)		probably benign	Het
Ccdc96	T	G	5: 36,643,707 (GRCm39)	V571G	probably damaging	Het
Col6a5	C	A	9: 105,814,544 (GRCm39)	E489D	unknown	Het
Dchs2	T	A	3: 83,176,577 (GRCm39)	I845N	probably damaging	Het
Dnajc11	T	C	4: 152,063,751 (GRCm39)	V449A	probably damaging	Het
Ect2	A	T	3: 27,193,268 (GRCm39)	Y269*	probably null	Het
Egfr	T	C	11: 16,819,294 (GRCm39)	Y275H	probably benign	Het
Fan1	T	A	7: 64,004,129 (GRCm39)	D779V	probably damaging	Het
Fancm	T	C	12: 65,123,942 (GRCm39)	V200A	probably damaging	Het
Frem1	A	G	4: 82,884,253 (GRCm39)	I1214T	probably benign	Het
Gm3415	T	A	5: 146,494,752 (GRCm39)	F138L	probably benign	Het
Gtse1	C	A	15: 85,759,370 (GRCm39)	T626K	probably benign	Het
Herc1	T	A	9: 66,393,464 (GRCm39)	H4114Q	probably damaging	Het
Ifi44	T	A	3: 151,454,826 (GRCm39)	N133I	probably benign	Het
Ilf3	A	G	9: 21,314,447 (GRCm39)		probably benign	Het
Inpp4a	A	G	1: 37,437,919 (GRCm39)	T593A	probably damaging	Het
Iqgap2	A	T	13: 95,819,441 (GRCm39)	W634R	possibly damaging	Het
Kcnh4	T	A	11: 100,641,105 (GRCm39)	N448I	probably damaging	Het
Klhl33	G	A	14: 51,130,564 (GRCm39)	A310V	probably damaging	Het
Klra9	G	T	6: 130,155,995 (GRCm39)	Y253*	probably null	Het
Lrig2	T	C	3: 104,398,863 (GRCm39)	K222E	possibly damaging	Het
Manba	G	A	3: 135,217,734 (GRCm39)		probably null	Het
Mga	A	T	2: 119,754,419 (GRCm39)	Q976L	probably damaging	Het
Mtmr4	A	G	11: 87,504,309 (GRCm39)	D1086G	probably damaging	Het
Muc20	A	G	16: 32,615,176 (GRCm39)	V67A	probably benign	Het
Myh3	T	A	11: 66,973,193 (GRCm39)	L97Q	probably damaging	Het
Nars1	T	C	18: 64,640,872 (GRCm39)	T195A	probably benign	Het
Npat	A	T	9: 53,474,739 (GRCm39)	I844L	possibly damaging	Het
Nrxn3	T	C	12: 88,762,285 (GRCm39)	F111L	probably benign	Het
Obp2b	T	C	2: 25,628,599 (GRCm39)	Y118H	probably damaging	Het
Or5w1	T	A	2: 87,486,558 (GRCm39)	K236*	probably null	Het
Patj	A	G	4: 98,379,866 (GRCm39)	D215G	probably damaging	Het
Pex1	T	A	5: 3,680,196 (GRCm39)	Y979*	probably null	Het
Septin3	G	A	15: 82,163,804 (GRCm39)	V54I	possibly damaging	Het
Shc3	A	G	13: 51,603,326 (GRCm39)	Y260H	probably damaging	Het
Simc1	A	G	13: 54,674,477 (GRCm39)	T942A	probably benign	Het
Slco1c1	T	C	6: 141,493,576 (GRCm39)	S371P	probably damaging	Het
Snrnp200	T	A	2: 127,080,574 (GRCm39)	I2029N	probably damaging	Het
Syne1	A	G	10: 5,268,422 (GRCm39)	V2089A	probably benign	Het
Syne2	T	C	12: 76,088,230 (GRCm39)	S5016P	probably damaging	Het
Tril	G	C	6: 53,795,493 (GRCm39)	D576E	probably damaging	Het
Tubb2b	C	T	13: 34,311,561 (GRCm39)	A411T	probably damaging	Het
Ubr4	T	C	4: 139,156,949 (GRCm39)	Y2325H	probably damaging	Het
Utp20	A	T	10: 88,608,395 (GRCm39)	C1547*	probably null	Het
Utrn	A	T	10: 12,401,171 (GRCm39)	W98R	probably damaging	Het
Vps35	C	T	8: 86,000,124 (GRCm39)	D501N	possibly damaging	Het
Wdr48	T	C	9: 119,745,879 (GRCm39)	S421P	possibly damaging	Het
Yju2b	A	G	8: 84,989,630 (GRCm39)	I63T	probably damaging	Het
Zfp719	C	A	7: 43,240,412 (GRCm39)	H667N	probably damaging	Het

Other mutations in Or7e170

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01114:Or7e170	APN	9	19,794,844 (GRCm39)	nonsense	probably null
IGL01636:Or7e170	APN	9	19,795,484 (GRCm39)	missense	probably benign	0.14
IGL01765:Or7e170	APN	9	19,795,247 (GRCm39)	missense	possibly damaging	0.71
IGL01967:Or7e170	APN	9	19,794,885 (GRCm39)	missense	probably damaging	0.97
IGL03145:Or7e170	APN	9	19,794,735 (GRCm39)	missense	possibly damaging	0.93
IGL03222:Or7e170	APN	9	19,795,495 (GRCm39)	nonsense	probably null
R0117:Or7e170	UTSW	9	19,795,595 (GRCm39)	missense	probably damaging	0.96
R0662:Or7e170	UTSW	9	19,795,248 (GRCm39)	missense	probably benign	0.32
R2399:Or7e170	UTSW	9	19,795,220 (GRCm39)	missense	probably damaging	0.98
R4224:Or7e170	UTSW	9	19,794,896 (GRCm39)	missense	probably benign	0.44
R4572:Or7e170	UTSW	9	19,795,275 (GRCm39)	missense	probably benign
R5607:Or7e170	UTSW	9	19,795,272 (GRCm39)	missense	probably benign	0.16
R5741:Or7e170	UTSW	9	19,794,857 (GRCm39)	missense	possibly damaging	0.92
R5759:Or7e170	UTSW	9	19,795,484 (GRCm39)	missense	probably benign	0.14
R6237:Or7e170	UTSW	9	19,795,365 (GRCm39)	missense	probably damaging	1.00
R7075:Or7e170	UTSW	9	19,795,359 (GRCm39)	missense	probably benign	0.16
R7534:Or7e170	UTSW	9	19,795,472 (GRCm39)	missense	probably benign	0.16
R7735:Or7e170	UTSW	9	19,795,410 (GRCm39)	missense	probably damaging	0.99
R8415:Or7e170	UTSW	9	19,795,409 (GRCm39)	missense	possibly damaging	0.95
R9246:Or7e170	UTSW	9	19,795,686 (GRCm39)	start gained	probably benign
R9438:Or7e170	UTSW	9	19,795,083 (GRCm39)	missense	probably benign
R9795:Or7e170	UTSW	9	19,795,347 (GRCm39)	missense	probably benign
Z1177:Or7e170	UTSW	9	19,794,963 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAACTGACAGGTGAGATCCAC -3'
(R):5'- ATGCAGCCTGCCTAACTCAG -3'

Sequencing Primer
(F):5'- CAGGTAGAGAATGCTTTATATTTCCC -3'
(R):5'- AACTCAGGTGTCTTTCTTCACTC -3'

Posted On

2018-05-24