Incidental Mutation 'R6435:Itch'
ID 518736
Institutional Source Beutler Lab
Gene Symbol Itch
Ensembl Gene ENSMUSG00000027598
Gene Name itchy, E3 ubiquitin protein ligase
Synonyms 6720481N21Rik, C230047C07Rik, 8030492O04Rik, AIP4
MMRRC Submission 044573-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R6435 (G1)
Quality Score 225.009
Status Validated
Chromosome 2
Chromosomal Location 154975429-155068775 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to A at 155051049 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Aspartic acid to Asparagine at position 579 (D579N)
Ref Sequence ENSEMBL: ENSMUSP00000105307 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000029126] [ENSMUST00000109685]
AlphaFold Q8C863
Predicted Effect probably benign
Transcript: ENSMUST00000029126
AA Change: D579N

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)
SMART Domains Protein: ENSMUSP00000029126
Gene: ENSMUSG00000027598
AA Change: D579N

DomainStartEndE-ValueType
C2 19 114 3.56e-12 SMART
low complexity region 195 206 N/A INTRINSIC
low complexity region 209 226 N/A INTRINSIC
low complexity region 230 259 N/A INTRINSIC
WW 288 320 1.07e-12 SMART
WW 321 352 3.86e-10 SMART
WW 400 432 7.36e-16 SMART
WW 440 472 6.82e-11 SMART
HECTc 528 864 7.04e-179 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000109685
AA Change: D579N

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)
SMART Domains Protein: ENSMUSP00000105307
Gene: ENSMUSG00000027598
AA Change: D579N

DomainStartEndE-ValueType
C2 19 114 3.56e-12 SMART
low complexity region 195 206 N/A INTRINSIC
low complexity region 209 226 N/A INTRINSIC
low complexity region 230 259 N/A INTRINSIC
WW 288 320 1.07e-12 SMART
WW 321 352 3.86e-10 SMART
WW 400 432 7.36e-16 SMART
WW 440 472 6.82e-11 SMART
HECTc 528 864 7.04e-179 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000123497
Meta Mutation Damage Score 0.1053 question?
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.1%
  • 20x: 94.2%
Validation Efficiency 100% (32/32)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
PHENOTYPE: Mice homozygous for an ENU mutation exhibit increased total IgE levels in the peripheral blood and an enhanced IgE response to the cysteine protease allergen, papain. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 33 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abl1 A T 2: 31,691,561 (GRCm39) T1027S possibly damaging Het
Ablim1 T C 19: 57,049,787 (GRCm39) I159V possibly damaging Het
Adcy1 A G 11: 7,111,367 (GRCm39) N886S possibly damaging Het
Baz1b G A 5: 135,266,799 (GRCm39) D1203N probably damaging Het
Chmp2a A T 7: 12,766,701 (GRCm39) Y72* probably null Het
Col6a1 A T 10: 76,546,957 (GRCm39) I755N unknown Het
Defa24 T A 8: 22,224,690 (GRCm39) F46L possibly damaging Het
Eml2 G A 7: 18,935,088 (GRCm39) V432I probably damaging Het
Gcn1 A T 5: 115,749,081 (GRCm39) probably null Het
Gfpt2 A G 11: 49,726,478 (GRCm39) S628G probably benign Het
Hsdl2 C A 4: 59,610,668 (GRCm39) Q222K unknown Het
Ighv1-9 T A 12: 114,547,206 (GRCm39) I111F probably benign Het
Jdp2 A T 12: 85,655,132 (GRCm39) K65M probably damaging Het
Kif15 A T 9: 122,815,556 (GRCm39) E222V probably damaging Het
Map1b A G 13: 99,652,871 (GRCm39) V42A probably damaging Het
Mecom T A 3: 30,034,398 (GRCm39) E426V probably damaging Het
Meiosin T C 7: 18,834,844 (GRCm39) probably benign Het
Mfsd6 G T 1: 52,748,603 (GRCm39) Y87* probably null Het
Mybpc2 A G 7: 44,155,481 (GRCm39) V924A possibly damaging Het
Naip6 A T 13: 100,431,249 (GRCm39) M1196K probably benign Het
Nscme3l T C 19: 5,553,446 (GRCm39) I112V probably benign Het
Or52n5 A G 7: 104,588,041 (GRCm39) M103V probably damaging Het
Pcdh9 G A 14: 94,125,280 (GRCm39) P297S probably benign Het
Pold1 G A 7: 44,188,202 (GRCm39) R559C probably damaging Het
Rgs21 A G 1: 144,395,565 (GRCm39) F108L probably damaging Het
Runx1 A T 16: 92,441,183 (GRCm39) S209T possibly damaging Het
Sdccag8 G T 1: 176,642,428 (GRCm39) probably benign Het
Smc1b C T 15: 84,976,232 (GRCm39) R825Q probably benign Het
Srgap1 T C 10: 121,636,732 (GRCm39) D741G possibly damaging Het
Srsf11 C T 3: 157,728,981 (GRCm39) probably benign Homo
Tsc1 G A 2: 28,566,464 (GRCm39) V627M probably benign Het
Vmn2r106 C T 17: 20,488,725 (GRCm39) C558Y probably damaging Het
Zfp612 A G 8: 110,815,952 (GRCm39) I347M probably damaging Het
Other mutations in Itch
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00481:Itch APN 2 155,054,943 (GRCm39) missense probably damaging 1.00
IGL00796:Itch APN 2 155,051,002 (GRCm39) missense probably damaging 0.97
IGL01090:Itch APN 2 155,048,256 (GRCm39) missense probably damaging 0.99
IGL01568:Itch APN 2 155,054,382 (GRCm39) splice site probably benign
IGL01844:Itch APN 2 155,014,406 (GRCm39) missense possibly damaging 0.94
IGL01844:Itch APN 2 155,014,467 (GRCm39) missense possibly damaging 0.56
IGL01873:Itch APN 2 155,010,670 (GRCm39) missense possibly damaging 0.68
IGL02129:Itch APN 2 155,059,908 (GRCm39) splice site probably benign
IGL02386:Itch APN 2 155,044,181 (GRCm39) nonsense probably null
IGL02545:Itch APN 2 155,014,506 (GRCm39) splice site probably null
IGL02621:Itch APN 2 155,014,504 (GRCm39) splice site probably null
IGL02708:Itch APN 2 155,015,964 (GRCm39) missense probably benign 0.00
IGL02869:Itch APN 2 155,015,853 (GRCm39) critical splice acceptor site probably null
Abrade UTSW 2 155,050,998 (GRCm39) missense possibly damaging 0.93
dorsolateral UTSW 2 155,052,478 (GRCm39) nonsense probably null
gadfly UTSW 2 155,024,218 (GRCm39) nonsense probably null
hankerin UTSW 2 155,052,502 (GRCm39) critical splice donor site probably null
irresistable UTSW 2 155,045,217 (GRCm39) missense probably benign 0.34
prurient UTSW 2 155,052,422 (GRCm39) missense probably damaging 1.00
scratch UTSW 2 155,014,481 (GRCm39) missense probably damaging 0.99
R0116:Itch UTSW 2 155,059,903 (GRCm39) splice site probably benign
R0207:Itch UTSW 2 155,044,177 (GRCm39) missense probably benign
R0226:Itch UTSW 2 155,041,314 (GRCm39) missense probably benign 0.01
R0545:Itch UTSW 2 155,024,218 (GRCm39) nonsense probably null
R0689:Itch UTSW 2 155,024,098 (GRCm39) missense possibly damaging 0.82
R1365:Itch UTSW 2 155,054,951 (GRCm39) missense probably benign 0.00
R1406:Itch UTSW 2 155,048,274 (GRCm39) missense possibly damaging 0.95
R1406:Itch UTSW 2 155,048,274 (GRCm39) missense possibly damaging 0.95
R1436:Itch UTSW 2 155,034,065 (GRCm39) missense probably damaging 0.96
R1639:Itch UTSW 2 155,020,945 (GRCm39) splice site probably null
R1769:Itch UTSW 2 155,014,481 (GRCm39) missense probably damaging 0.99
R1855:Itch UTSW 2 155,014,374 (GRCm39) splice site probably benign
R1865:Itch UTSW 2 155,010,666 (GRCm39) missense probably damaging 0.96
R2008:Itch UTSW 2 155,052,379 (GRCm39) missense possibly damaging 0.91
R2054:Itch UTSW 2 155,052,496 (GRCm39) missense probably damaging 1.00
R2196:Itch UTSW 2 155,044,141 (GRCm39) missense probably benign
R2199:Itch UTSW 2 155,044,141 (GRCm39) missense probably benign
R2252:Itch UTSW 2 155,054,259 (GRCm39) missense probably benign 0.01
R2253:Itch UTSW 2 155,054,259 (GRCm39) missense probably benign 0.01
R2348:Itch UTSW 2 155,050,998 (GRCm39) missense possibly damaging 0.93
R2850:Itch UTSW 2 155,044,141 (GRCm39) missense probably benign
R3021:Itch UTSW 2 155,051,046 (GRCm39) missense possibly damaging 0.74
R4676:Itch UTSW 2 155,041,355 (GRCm39) missense probably benign 0.05
R4716:Itch UTSW 2 155,052,502 (GRCm39) critical splice donor site probably null
R4888:Itch UTSW 2 155,059,897 (GRCm39) splice site probably null
R4970:Itch UTSW 2 155,027,513 (GRCm39) missense possibly damaging 0.50
R6029:Itch UTSW 2 155,021,009 (GRCm39) critical splice donor site probably null
R6122:Itch UTSW 2 155,015,985 (GRCm39) missense probably benign 0.05
R6449:Itch UTSW 2 155,005,315 (GRCm39) splice site probably benign
R7069:Itch UTSW 2 155,051,914 (GRCm39) missense probably damaging 1.00
R7083:Itch UTSW 2 155,052,364 (GRCm39) missense probably damaging 1.00
R7409:Itch UTSW 2 155,041,302 (GRCm39) missense probably damaging 0.99
R7689:Itch UTSW 2 155,054,987 (GRCm39) missense probably benign 0.00
R7689:Itch UTSW 2 155,051,922 (GRCm39) missense probably damaging 0.99
R7974:Itch UTSW 2 155,034,079 (GRCm39) missense probably damaging 1.00
R8046:Itch UTSW 2 155,052,422 (GRCm39) missense probably damaging 1.00
R8248:Itch UTSW 2 155,048,303 (GRCm39) critical splice donor site probably null
R8355:Itch UTSW 2 155,052,502 (GRCm39) critical splice donor site probably null
R8428:Itch UTSW 2 155,010,627 (GRCm39) missense probably benign 0.38
R8691:Itch UTSW 2 155,052,478 (GRCm39) nonsense probably null
R8779:Itch UTSW 2 155,014,440 (GRCm39) missense probably benign 0.28
R9010:Itch UTSW 2 155,020,991 (GRCm39) missense probably benign
R9130:Itch UTSW 2 155,052,045 (GRCm39) splice site probably benign
R9278:Itch UTSW 2 155,045,217 (GRCm39) missense probably benign 0.34
Z1177:Itch UTSW 2 155,050,979 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TGTGAGCAGGTTCCTGACAG -3'
(R):5'- GAACTCACTTTCCCTAAAGGTCC -3'

Sequencing Primer
(F):5'- AGGTTCCTGACAGCTCTGTAACAC -3'
(R):5'- GCAGAAGATCCTGGTTCATTCTCAG -3'
Posted On 2018-05-24