Mutagenetix > Incidental Mutations

Incidental Mutation 'R6436:Gm35315'

518771

Institutional Source

Beutler Lab

Gene Symbol

Gm35315

Ensembl Gene

ENSMUSG00000109771

Gene Name

predicted gene, 35315

Synonyms

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.084) question?

Stock #

R6436 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

110076136-110094420 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 110078712 bp

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 287 (T287I)

Ref Sequence

ENSEMBL: ENSMUSP00000147534 (fasta)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000209865

Predicted Effect

probably benign
Transcript: ENSMUST00000210052
AA Change: T287I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Predicted Effect

probably benign
Transcript: ENSMUST00000211397

Coding Region Coverage

1x: 100.0%
3x: 99.7%
10x: 98.1%
20x: 93.5%

Validation Efficiency

100% (33/33)

Allele List at MGI

Other mutations in this stock

Total: 32 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arc	C	A	15: 74,672,249	V42L	possibly damaging	Het
Clip1	C	T	5: 123,641,785	E433K	probably damaging	Het
Cmya5	T	C	13: 93,089,215	T3122A	probably damaging	Het
Ctf2	G	T	7: 127,719,431	A92E	probably damaging	Het
Ctsw	G	T	19: 5,466,294	R184S	possibly damaging	Het
D130043K22Rik	A	G	13: 24,877,935	E629G	probably damaging	Het
Dmbt1	T	C	7: 131,116,641	V1523A	probably benign	Het
Eml2	G	A	7: 19,201,163	V432I	probably damaging	Het
Fanci	T	C	7: 79,440,698	I982T	probably benign	Het
Fnbp1	T	C	2: 31,096,127	D3G	probably damaging	Het
Gm10770	G	A	2: 150,178,910	T229I	probably benign	Het
Itgb3	T	A	11: 104,633,492	D151E	probably damaging	Het
Lcn8	A	T	2: 25,654,978		probably null	Het
Ninl	A	G	2: 150,966,178	L310P	probably damaging	Het
Nop2	A	G	6: 125,137,311	D215G	probably benign	Het
Olfr1426	T	C	19: 12,087,935	T286A	probably benign	Het
Olfr788	A	G	10: 129,472,904	T71A	probably damaging	Het
Parg	T	C	14: 32,271,677	W289R	probably damaging	Het
Pdcd4	A	G	19: 53,926,931		probably null	Het
Plk2	G	T	13: 110,396,036	E95*	probably null	Het
Pold1	G	A	7: 44,538,778	R559C	probably damaging	Het
Ptprc	T	C	1: 138,083,639	D560G	possibly damaging	Het
Rasal3	T	C	17: 32,397,504	Y290C	probably damaging	Het
Rcsd1	T	A	1: 165,657,615	S90C	probably damaging	Het
Rttn	T	C	18: 89,110,729	L1935P	probably damaging	Het
Smc1b	C	T	15: 85,092,031	R825Q	probably benign	Het
Srsf11	C	T	3: 158,023,344		probably benign	Homo
Tmc3	T	C	7: 83,598,487	Y230H	probably damaging	Het
Tnfrsf25	A	T	4: 152,119,627		probably null	Het
Ubl7	T	C	9: 57,920,510	L160P	probably damaging	Het
Vmn2r106	C	T	17: 20,268,463	C558Y	probably damaging	Het
Zfp646	T	A	7: 127,879,941	V430E	probably benign	Het

Other mutations in Gm35315

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R6103:Gm35315	UTSW	5	110078271	missense	probably damaging	0.97
R6219:Gm35315	UTSW	5	110078544	missense	probably benign	0.23
R6429:Gm35315	UTSW	5	110078659	missense	possibly damaging	0.67
R6482:Gm35315	UTSW	5	110078089	missense	possibly damaging	0.92
R6632:Gm35315	UTSW	5	110079263	nonsense	probably null
R7313:Gm35315	UTSW	5	110079225	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- ATGCAAAGGCCTTACCACATTGAT -3'
(R):5'- AAGCCTTTGCATTTCCTGGTCA -3'

Sequencing Primer
(F):5'- CCACATTGATCACATTCATAGGG -3'
(R):5'- CCTGGTCATCTTCAAAGACATG -3'

Posted On

2018-05-24