Mutagenetix > Incidental Mutation

Incidental Mutation 'R6441:Vmn2r28'

519013

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r28

Ensembl Gene

ENSMUSG00000066820

Gene Name

vomeronasal 2, receptor 28

Synonyms

EG665255

MMRRC Submission

044579-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.124) question?

Stock #

R6441 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

5483455-5496850 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 5491474 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 258 (M258L)

Ref Sequence

ENSEMBL: ENSMUSP00000083477 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086297]

AlphaFold

L7N203

Predicted Effect

probably benign
Transcript: ENSMUST00000086297
AA Change: M258L

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000083477
Gene: ENSMUSG00000066820
AA Change: M258L

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	73	470	1.7e-26	PFAM
Pfam:NCD3G	512	565	9.8e-21	PFAM
Pfam:7tm_3	598	833	2.8e-56	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 97.9%
20x: 93.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Agl	A	T	3: 116,565,108 (GRCm39)	M1047K	probably benign	Het
Apob	A	T	12: 8,037,796 (GRCm39)	D322V	probably damaging	Het
Capn12	T	A	7: 28,587,427 (GRCm39)	C438S	probably benign	Het
Cdh23	A	T	10: 60,143,815 (GRCm39)	V2932E	probably damaging	Het
Cdh9	A	T	15: 16,823,509 (GRCm39)	T164S	probably benign	Het
Cimap1a	T	C	7: 140,429,161 (GRCm39)	S149P	probably damaging	Het
CN725425	T	C	15: 91,120,005 (GRCm39)	V42A	probably benign	Het
Cpb1	T	C	3: 20,303,978 (GRCm39)	D362G	probably damaging	Het
Csmd2	C	A	4: 128,288,757 (GRCm39)	Q1099K	probably benign	Het
D6Ertd527e	C	G	6: 87,088,506 (GRCm39)	T223S	unknown	Het
Galnt4	A	G	10: 98,945,960 (GRCm39)	M562V	possibly damaging	Het
Gtf3c3	T	A	1: 54,445,197 (GRCm39)	E619V	probably benign	Het
Gucy2c	A	T	6: 136,700,759 (GRCm39)	M585K	probably damaging	Het
Hmcn1	A	G	1: 150,578,967 (GRCm39)	I1993T	possibly damaging	Het
Lonrf2	T	C	1: 38,857,204 (GRCm39)	E44G	possibly damaging	Het
Lrit3	A	T	3: 129,594,009 (GRCm39)	F189L	probably benign	Het
Mag	T	C	7: 30,606,508 (GRCm39)	N310D	possibly damaging	Het
Mccc2	T	C	13: 100,091,184 (GRCm39)	T151A	probably damaging	Het
Mybpc1	A	T	10: 88,389,139 (GRCm39)	S49T	probably benign	Het
Myh2	A	G	11: 67,085,437 (GRCm39)	E1787G	probably benign	Het
Ncapd3	A	G	9: 26,974,712 (GRCm39)	D728G	probably benign	Het
Nup37	A	G	10: 87,996,799 (GRCm39)	E139G	probably benign	Het
Or52h9	C	T	7: 104,202,542 (GRCm39)	Q139*	probably null	Het
Pcdhgb5	A	G	18: 37,865,138 (GRCm39)	Y311C	probably damaging	Het
Pls1	A	G	9: 95,636,798 (GRCm39)	I558T	probably damaging	Het
Rbm44	A	T	1: 91,084,799 (GRCm39)	N674Y	probably damaging	Het
Ryr1	T	C	7: 28,759,120 (GRCm39)	I3353V	possibly damaging	Het
Sema3c	C	T	5: 17,929,130 (GRCm39)	T588I	possibly damaging	Het
Sh2d5	A	T	4: 137,986,393 (GRCm39)	E372V	possibly damaging	Het
Slc27a6	C	T	18: 58,705,130 (GRCm39)	P171S	probably benign	Het
Slfn3	C	T	11: 83,105,740 (GRCm39)	T579I	probably benign	Het
Svil	T	G	18: 5,049,323 (GRCm39)	V287G	probably benign	Het
Tas1r2	T	A	4: 139,396,467 (GRCm39)	V602D	probably damaging	Het
Tln2	A	G	9: 67,179,971 (GRCm39)	L800P	probably damaging	Het
Trim31	C	A	17: 37,218,683 (GRCm39)	Q323K	possibly damaging	Het
Txndc9	T	A	1: 38,029,299 (GRCm39)	D183V	possibly damaging	Het
Vmn2r75	A	T	7: 85,820,784 (GRCm39)	I50N	probably damaging	Het
Zgrf1	A	T	3: 127,381,683 (GRCm39)	N1161Y	possibly damaging	Het

Other mutations in Vmn2r28

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Vmn2r28	APN	7	5,491,068 (GRCm39)	missense	probably benign	0.12
IGL01061:Vmn2r28	APN	7	5,491,183 (GRCm39)	missense	probably damaging	1.00
IGL01160:Vmn2r28	APN	7	5,489,477 (GRCm39)	missense	probably damaging	0.99
IGL01335:Vmn2r28	APN	7	5,484,087 (GRCm39)	missense	possibly damaging	0.67
IGL01532:Vmn2r28	APN	7	5,489,463 (GRCm39)	missense	probably benign	0.16
IGL01791:Vmn2r28	APN	7	5,491,161 (GRCm39)	missense	probably benign	0.00
IGL01875:Vmn2r28	APN	7	5,484,302 (GRCm39)	missense	probably benign	0.30
IGL02161:Vmn2r28	APN	7	5,491,123 (GRCm39)	missense	possibly damaging	0.88
IGL02499:Vmn2r28	APN	7	5,493,568 (GRCm39)	missense	probably damaging	0.98
IGL02858:Vmn2r28	APN	7	5,484,003 (GRCm39)	missense	probably damaging	0.99
IGL03061:Vmn2r28	APN	7	5,487,015 (GRCm39)	missense	probably damaging	0.98
G1patch:Vmn2r28	UTSW	7	5,491,408 (GRCm39)	missense	probably benign	0.13
R0288:Vmn2r28	UTSW	7	5,491,020 (GRCm39)	missense	probably damaging	1.00
R0361:Vmn2r28	UTSW	7	5,496,715 (GRCm39)	missense	probably benign	0.00
R0396:Vmn2r28	UTSW	7	5,491,513 (GRCm39)	missense	probably benign	0.05
R0480:Vmn2r28	UTSW	7	5,493,456 (GRCm39)	missense	probably benign	0.00
R0485:Vmn2r28	UTSW	7	5,491,689 (GRCm39)	missense	probably damaging	1.00
R0837:Vmn2r28	UTSW	7	5,491,026 (GRCm39)	missense	probably damaging	0.99
R1282:Vmn2r28	UTSW	7	5,484,301 (GRCm39)	missense	probably damaging	0.99
R1296:Vmn2r28	UTSW	7	5,484,544 (GRCm39)	missense	possibly damaging	0.81
R1829:Vmn2r28	UTSW	7	5,496,810 (GRCm39)	missense	probably benign
R1853:Vmn2r28	UTSW	7	5,484,246 (GRCm39)	nonsense	probably null
R1869:Vmn2r28	UTSW	7	5,489,345 (GRCm39)	missense	probably benign	0.00
R1887:Vmn2r28	UTSW	7	5,491,288 (GRCm39)	missense	possibly damaging	0.90
R1961:Vmn2r28	UTSW	7	5,484,070 (GRCm39)	missense	possibly damaging	0.85
R1998:Vmn2r28	UTSW	7	5,491,313 (GRCm39)	missense	possibly damaging	0.87
R2392:Vmn2r28	UTSW	7	5,487,130 (GRCm39)	missense	probably damaging	0.98
R2432:Vmn2r28	UTSW	7	5,491,701 (GRCm39)	missense	probably damaging	0.99
R3055:Vmn2r28	UTSW	7	5,484,391 (GRCm39)	missense	probably damaging	0.98
R3753:Vmn2r28	UTSW	7	5,491,026 (GRCm39)	missense	probably damaging	0.99
R3877:Vmn2r28	UTSW	7	5,491,357 (GRCm39)	missense	probably damaging	1.00
R4307:Vmn2r28	UTSW	7	5,493,707 (GRCm39)	missense	probably damaging	0.99
R5023:Vmn2r28	UTSW	7	5,489,463 (GRCm39)	missense	probably benign	0.16
R5057:Vmn2r28	UTSW	7	5,489,463 (GRCm39)	missense	probably benign	0.16
R5083:Vmn2r28	UTSW	7	5,483,671 (GRCm39)	missense	possibly damaging	0.77
R5427:Vmn2r28	UTSW	7	5,489,376 (GRCm39)	missense	probably damaging	0.99
R5472:Vmn2r28	UTSW	7	5,490,943 (GRCm39)	critical splice donor site	probably null
R5511:Vmn2r28	UTSW	7	5,487,011 (GRCm39)	missense	possibly damaging	0.61
R5731:Vmn2r28	UTSW	7	5,491,668 (GRCm39)	missense	probably benign	0.41
R6091:Vmn2r28	UTSW	7	5,496,790 (GRCm39)	missense	possibly damaging	0.88
R6179:Vmn2r28	UTSW	7	5,491,003 (GRCm39)	nonsense	probably null
R6276:Vmn2r28	UTSW	7	5,493,730 (GRCm39)	missense	probably benign	0.01
R6463:Vmn2r28	UTSW	7	5,489,435 (GRCm39)	missense	probably benign	0.07
R6528:Vmn2r28	UTSW	7	5,493,684 (GRCm39)	missense	probably benign	0.12
R6725:Vmn2r28	UTSW	7	5,491,408 (GRCm39)	missense	probably benign	0.13
R6760:Vmn2r28	UTSW	7	5,484,229 (GRCm39)	missense	probably damaging	0.97
R6849:Vmn2r28	UTSW	7	5,483,806 (GRCm39)	missense	probably damaging	1.00
R7110:Vmn2r28	UTSW	7	5,493,733 (GRCm39)	missense	probably benign	0.06
R7146:Vmn2r28	UTSW	7	5,484,495 (GRCm39)	missense	probably benign	0.05
R7407:Vmn2r28	UTSW	7	5,484,308 (GRCm39)	missense	probably damaging	1.00
R7563:Vmn2r28	UTSW	7	5,491,200 (GRCm39)	missense	probably benign	0.00
R7611:Vmn2r28	UTSW	7	5,484,255 (GRCm39)	missense	probably benign	0.02
R7808:Vmn2r28	UTSW	7	5,496,678 (GRCm39)	missense	probably damaging	0.98
R7862:Vmn2r28	UTSW	7	5,493,613 (GRCm39)	missense	probably benign	0.00
R7916:Vmn2r28	UTSW	7	5,483,818 (GRCm39)	missense	probably damaging	1.00
R8183:Vmn2r28	UTSW	7	5,491,147 (GRCm39)	missense	probably damaging	1.00
R8334:Vmn2r28	UTSW	7	5,487,059 (GRCm39)	missense	probably damaging	1.00
R8519:Vmn2r28	UTSW	7	5,489,347 (GRCm39)	missense	probably benign	0.31
R8989:Vmn2r28	UTSW	7	5,493,473 (GRCm39)	missense	probably benign	0.00
R9066:Vmn2r28	UTSW	7	5,491,596 (GRCm39)	missense	probably damaging	1.00
R9422:Vmn2r28	UTSW	7	5,483,747 (GRCm39)	missense	probably damaging	1.00
R9469:Vmn2r28	UTSW	7	5,487,118 (GRCm39)	missense	probably damaging	0.99
R9608:Vmn2r28	UTSW	7	5,491,220 (GRCm39)	missense	probably benign	0.05

Predicted Primers

PCR Primer
(F):5'- AAATCTCAGCATGGTGGGG -3'
(R):5'- GCATTGGCAATGGTGTACTTC -3'

Sequencing Primer
(F):5'- AGTCCCATAGAATGTGCCATG -3'
(R):5'- CATACTTTACTTCCAATGGAACTGGG -3'

Posted On

2018-05-24