Mutagenetix > Incidental Mutations

Incidental Mutation 'R6533:Vmn2r4'

520172

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r4

Ensembl Gene

ENSMUSG00000092049

Gene Name

vomeronasal 2, receptor 4

Synonyms

EG637053

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.063) question?

Stock #

R6533 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

64388621-64415296 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 64415098 bp

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 67 (T67A)

Ref Sequence

ENSEMBL: ENSMUSP00000135228 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000175724]

Predicted Effect

probably benign
Transcript: ENSMUST00000175724
AA Change: T67A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000135228
Gene: ENSMUSG00000092049
AA Change: T67A

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	88	505	2.3e-75	PFAM
Pfam:NCD3G	547	600	4.7e-17	PFAM
Pfam:7tm_3	633	867	8.2e-47	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 98.2%

Validation Efficiency

100% (44/44)

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700010B08Rik	C	T	2: 173,719,835		probably benign	Het
4932414N04Rik	G	T	2: 68,716,318	E115*	probably null	Het
Abhd16a	G	A	17: 35,098,809		probably null	Het
Ankrd28	T	A	14: 31,732,084	I244L	possibly damaging	Het
Barx2	T	C	9: 31,912,979	Y38C	probably damaging	Het
Btn2a2	C	A	13: 23,481,781	E294*	probably null	Het
Ceacam2	C	G	7: 25,530,711	V157L	probably benign	Het
Ces2c	T	A	8: 104,852,093	F334L	possibly damaging	Het
Col7a1	T	C	9: 108,961,358	I958T	unknown	Het
Cyhr1	C	A	15: 76,647,730	E283*	probably null	Het
Dcp2	T	A	18: 44,399,664	D82E	probably benign	Het
Dnah8	T	C	17: 30,746,990	L2432S	probably damaging	Het
Dst	A	G	1: 34,303,509	D7582G	probably benign	Het
Fat3	T	A	9: 15,998,899	I1936L	probably benign	Het
Gsdmc4	T	A	15: 63,892,060	N396I	probably damaging	Het
Lonrf2	A	C	1: 38,813,268	D167E	probably benign	Het
Marf1	T	C	16: 14,115,799	D1575G	probably benign	Het
Med23	T	C	10: 24,893,620	L101P	probably damaging	Het
Myh3	A	G	11: 67,090,419	I703V	probably damaging	Het
Ncan	G	A	8: 70,096,357	A1257V	probably benign	Het
Nipal4	A	T	11: 46,150,407	Y320*	probably null	Het
Obox5	A	T	7: 15,757,607	Q24L	probably benign	Het
Orc1	T	C	4: 108,597,447	S345P	probably benign	Het
P4hb	A	T	11: 120,571,643	I79N	probably damaging	Het
Phf3	A	T	1: 30,806,318	I1262N	probably damaging	Het
Pigo	A	T	4: 43,022,697	N291K	probably benign	Het
Ppargc1b	C	T	18: 61,307,774	R691H	possibly damaging	Het
Ppm1m	T	C	9: 106,196,870		probably benign	Het
Ptprd	A	T	4: 76,128,528	D500E	probably damaging	Het
Rab3gap2	T	A	1: 185,232,954		probably null	Het
Rnf214	A	G	9: 45,900,063	S101P	probably benign	Het
Sdhc	A	G	1: 171,129,827	S162P	possibly damaging	Het
Spta1	C	T	1: 174,244,147	T2231I	probably damaging	Het
Stxbp2	T	A	8: 3,642,683	D578E	probably benign	Het
Tacc2	A	T	7: 130,622,837	E417D	possibly damaging	Het
Tas2r144	A	T	6: 42,215,346	N7Y	probably benign	Het
Tasp1	A	G	2: 139,834,357	*421R	probably null	Het
Tigd5	A	G	15: 75,910,190	I134V	possibly damaging	Het
Tmem95	A	T	11: 69,878,017	M1K	probably null	Het
Trappc10	T	C	10: 78,188,894	M1134V	probably damaging	Het
Unc45a	T	G	7: 80,334,069	K326N	probably damaging	Het
Vmn1r59	A	G	7: 5,454,464	V99A	probably benign	Het
Vmn2r85	A	T	10: 130,426,660	M70K	probably benign	Het
Zkscan8	A	G	13: 21,520,578	F325S	probably damaging	Het

Other mutations in Vmn2r4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01310:Vmn2r4	APN	3	64409779	splice site	probably null
IGL01448:Vmn2r4	APN	3	64406395	missense	probably damaging	0.99
IGL01452:Vmn2r4	APN	3	64406395	missense	probably damaging	0.99
IGL01454:Vmn2r4	APN	3	64406395	missense	probably damaging	0.99
IGL01456:Vmn2r4	APN	3	64406395	missense	probably damaging	0.99
IGL01463:Vmn2r4	APN	3	64406395	missense	probably damaging	0.99
IGL01467:Vmn2r4	APN	3	64406395	missense	probably damaging	0.99
IGL01468:Vmn2r4	APN	3	64406395	missense	probably damaging	0.99
IGL01470:Vmn2r4	APN	3	64406395	missense	probably damaging	0.99
IGL01476:Vmn2r4	APN	3	64406395	missense	probably damaging	0.99
IGL01481:Vmn2r4	APN	3	64406395	missense	probably damaging	0.99
IGL01534:Vmn2r4	APN	3	64406423	missense	probably damaging	1.00
IGL01636:Vmn2r4	APN	3	64406236	missense	probably benign	0.21
IGL01879:Vmn2r4	APN	3	64391010	missense	probably damaging	1.00
IGL02147:Vmn2r4	APN	3	64398361	splice site	probably benign
IGL02276:Vmn2r4	APN	3	64406456	missense	possibly damaging	0.95
IGL02432:Vmn2r4	APN	3	64406400	missense	probably benign	0.38
IGL02533:Vmn2r4	APN	3	64398419	nonsense	probably null
IGL02655:Vmn2r4	APN	3	64398465	missense	probably damaging	0.97
IGL02666:Vmn2r4	APN	3	64389012	missense	probably benign	0.10
IGL02902:Vmn2r4	APN	3	64406916	missense	probably benign	0.22
IGL03189:Vmn2r4	APN	3	64389168	missense	possibly damaging	0.89
IGL03250:Vmn2r4	APN	3	64406642	missense	probably damaging	1.00
IGL03271:Vmn2r4	APN	3	64398429	missense	probably benign	0.01
R0310:Vmn2r4	UTSW	3	64389434	nonsense	probably null
R0504:Vmn2r4	UTSW	3	64389363	missense	probably damaging	1.00
R1546:Vmn2r4	UTSW	3	64406888	missense	probably damaging	1.00
R1562:Vmn2r4	UTSW	3	64389444	missense	probably damaging	0.98
R1863:Vmn2r4	UTSW	3	64406989	missense	probably benign	0.33
R1873:Vmn2r4	UTSW	3	64391058	missense	possibly damaging	0.93
R1939:Vmn2r4	UTSW	3	64398555	missense	probably benign	0.00
R2103:Vmn2r4	UTSW	3	64415283	missense	possibly damaging	0.48
R3083:Vmn2r4	UTSW	3	64389367	missense	probably damaging	1.00
R3687:Vmn2r4	UTSW	3	64389475	missense	possibly damaging	0.93
R3707:Vmn2r4	UTSW	3	64389474	missense	probably damaging	0.99
R3963:Vmn2r4	UTSW	3	64415151	missense	probably damaging	0.99
R4428:Vmn2r4	UTSW	3	64415169	missense	probably damaging	1.00
R4710:Vmn2r4	UTSW	3	64409780	critical splice donor site	probably null
R4737:Vmn2r4	UTSW	3	64409963	missense	probably damaging	1.00
R4767:Vmn2r4	UTSW	3	64390976	missense	probably damaging	0.99
R4776:Vmn2r4	UTSW	3	64388661	missense	probably damaging	0.96
R4834:Vmn2r4	UTSW	3	64410063	missense	probably benign	0.40
R4893:Vmn2r4	UTSW	3	64406255	missense	probably damaging	0.96
R4908:Vmn2r4	UTSW	3	64389055	missense	possibly damaging	0.59
R5049:Vmn2r4	UTSW	3	64398598	splice site	probably null
R5092:Vmn2r4	UTSW	3	64390952	missense	probably benign	0.01
R5234:Vmn2r4	UTSW	3	64398457	missense	possibly damaging	0.88
R5240:Vmn2r4	UTSW	3	64406937	missense	possibly damaging	0.53
R5704:Vmn2r4	UTSW	3	64409949	missense	probably benign	0.03
R5897:Vmn2r4	UTSW	3	64415266	nonsense	probably null
R5907:Vmn2r4	UTSW	3	64391066	missense	probably damaging	0.99
R5924:Vmn2r4	UTSW	3	64389264	missense	probably damaging	1.00
R6145:Vmn2r4	UTSW	3	64406943	missense	probably benign	0.00
R6191:Vmn2r4	UTSW	3	64415281	missense	probably benign	0.34
R6192:Vmn2r4	UTSW	3	64415278	missense	probably benign	0.00
R6207:Vmn2r4	UTSW	3	64406505	missense	probably damaging	1.00
R6457:Vmn2r4	UTSW	3	64409957	missense	probably damaging	1.00
R6545:Vmn2r4	UTSW	3	64406356	missense	possibly damaging	0.50
R6594:Vmn2r4	UTSW	3	64389310	missense	probably damaging	1.00
R7049:Vmn2r4	UTSW	3	64389129	missense	probably benign	0.14
R7150:Vmn2r4	UTSW	3	64398477	missense	probably benign	0.01
R7187:Vmn2r4	UTSW	3	64415260	missense	probably benign	0.00
R7363:Vmn2r4	UTSW	3	64407011	missense	probably damaging	1.00
R7477:Vmn2r4	UTSW	3	64398429	missense	probably benign	0.01
R7675:Vmn2r4	UTSW	3	64415236	missense	probably benign	0.01
R7858:Vmn2r4	UTSW	3	64409805	missense	probably benign	0.00
R7888:Vmn2r4	UTSW	3	64406522	missense	probably damaging	0.99
R8678:Vmn2r4	UTSW	3	64406970	missense	probably benign
R8743:Vmn2r4	UTSW	3	64409826	missense	possibly damaging	0.95
X0019:Vmn2r4	UTSW	3	64406636	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GTCTCCATCCAGCATACTGC -3'
(R):5'- GCTCACCACATGAAAATGGC -3'

Sequencing Primer
(F):5'- TGCTGTCCCCATCATGAGATAAAGG -3'
(R):5'- GCAGAAATAAGTGCTTGATTCTGG -3'

Posted On

2018-06-06