Mutagenetix > Incidental Mutation

Incidental Mutation 'R6536:Tns3'

520425

Institutional Source

Beutler Lab

Gene Symbol

Tns3

Ensembl Gene

ENSMUSG00000020422

Gene Name

tensin 3

Synonyms

TEM6, F830010I22Rik, Tens1

MMRRC Submission

044662-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.371) question?

Stock #

R6536 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

8431652-8664535 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 8434531 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 1429 (V1429E)

Ref Sequence

ENSEMBL: ENSMUSP00000020695 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020695]

AlphaFold

Q5SSZ5

Predicted Effect

probably damaging
Transcript: ENSMUST00000020695
AA Change: V1429E

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000020695
Gene: ENSMUSG00000020422
AA Change: V1429E

Domain	Start	End	E-Value	Type
SCOP:d1d5ra2	1	171	5e-28	SMART
PTEN_C2	173	300	1.15e-48	SMART
low complexity region	854	864	N/A	INTRINSIC
low complexity region	1102	1126	N/A	INTRINSIC
SH2	1165	1268	1.32e-18	SMART
PTB	1301	1438	3.14e-24	SMART

Meta Mutation Damage Score

0.8017

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.4%

Validation Efficiency

100% (36/36)

MGI Phenotype

PHENOTYPE: Mice homozygous for a null allele exhibit one third postnatal lethality, reduced body weight, growth retardation, smaller digestive tracts with defects in villi and enterocyte differentiation, abnormal lung morphology, and thinner bones with decreased chondrocyte proliferation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9930111J21Rik2	G	T	11: 49,019,723 (GRCm38)	H628N	probably benign	Het
Abcb1a	T	A	5: 8,719,030 (GRCm38)	F751I	probably benign	Het
Adamts13	G	A	2: 26,975,750 (GRCm38)	V106M	probably damaging	Het
Add1	C	A	5: 34,601,436 (GRCm38)	N31K	possibly damaging	Het
Adgrf5	G	T	17: 43,422,661 (GRCm38)		probably benign	Het
Akap11	T	G	14: 78,511,314 (GRCm38)	D1211A	possibly damaging	Het
Atp5c1	G	A	2: 10,080,316 (GRCm38)		probably benign	Het
Cd320	T	C	17: 33,847,503 (GRCm38)	S72P	probably benign	Het
Clca4b	C	A	3: 144,916,729 (GRCm38)	W525L	possibly damaging	Het
Csmd3	G	A	15: 47,838,467 (GRCm38)	T1740I	probably damaging	Het
Dnah7c	T	G	1: 46,658,290 (GRCm38)	S2122A	probably benign	Het
Enpp2	T	C	15: 54,862,631 (GRCm38)	N583S	probably damaging	Het
Fuk	A	G	8: 110,883,879 (GRCm38)	V964A	possibly damaging	Het
Gpd2	A	T	2: 57,345,355 (GRCm38)	I366F	probably benign	Het
Hsd17b2	G	A	8: 117,702,182 (GRCm38)	V63M	possibly damaging	Het
Hsdl2	A	T	4: 59,610,508 (GRCm38)		probably null	Het
Idh3b	AG	AGCACCACAACTG	2: 130,279,673 (GRCm38)		probably null	Het
Ifi44	A	G	3: 151,732,489 (GRCm38)	V387A	probably benign	Het
Kcnc4	T	C	3: 107,448,196 (GRCm38)	D312G	possibly damaging	Het
Kif1b	T	C	4: 149,192,596 (GRCm38)	M1337V	probably benign	Het
Klra6	C	T	6: 130,023,719 (GRCm38)	V41I	probably benign	Het
Lrp1	A	G	10: 127,558,068 (GRCm38)		probably null	Het
Mpdz	T	C	4: 81,383,417 (GRCm38)	E257G	probably damaging	Het
Olfr1306	T	C	2: 111,912,774 (GRCm38)	D52G	possibly damaging	Het
Olfr1420	T	C	19: 11,896,396 (GRCm38)	V125A	probably benign	Het
Papln	A	G	12: 83,781,887 (GRCm38)	Y789C	probably damaging	Het
Pcdh15	T	C	10: 74,631,389 (GRCm38)	L1680P	probably damaging	Het
Pcdhac1	A	G	18: 37,090,314 (GRCm38)	N60S	probably benign	Het
Polr3g	T	C	13: 81,678,216 (GRCm38)	N162S	unknown	Het
Pou3f3	A	G	1: 42,698,214 (GRCm38)	I357V	probably damaging	Het
Sycp2	A	G	2: 178,351,648 (GRCm38)	S1235P	probably damaging	Het
Tdgf1	A	T	9: 110,944,189 (GRCm38)		probably null	Het
Trim67	T	C	8: 124,794,342 (GRCm38)	S148P	possibly damaging	Het
Usp49	A	T	17: 47,679,692 (GRCm38)	I348F	probably damaging	Het
Wac	A	T	18: 7,905,189 (GRCm38)		probably null	Het
Zfp775	A	G	6: 48,619,609 (GRCm38)	K139R	probably damaging	Het

Other mutations in Tns3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00163:Tns3	APN	11	8,451,066 (GRCm38)	missense	probably benign	0.42
IGL00822:Tns3	APN	11	8,443,976 (GRCm38)	missense	probably damaging	0.99
IGL01075:Tns3	APN	11	8,478,399 (GRCm38)	missense	probably benign	0.45
IGL01286:Tns3	APN	11	8,492,617 (GRCm38)	missense	probably benign	0.01
IGL01680:Tns3	APN	11	8,548,937 (GRCm38)	missense	probably damaging	1.00
IGL01687:Tns3	APN	11	8,492,798 (GRCm38)	missense	probably damaging	1.00
IGL01734:Tns3	APN	11	8,519,192 (GRCm38)	splice site	probably benign
IGL01844:Tns3	APN	11	8,437,177 (GRCm38)	missense	possibly damaging	0.58
IGL01984:Tns3	APN	11	8,548,992 (GRCm38)	nonsense	probably null
IGL02137:Tns3	APN	11	8,492,578 (GRCm38)	missense	possibly damaging	0.93
IGL02273:Tns3	APN	11	8,434,531 (GRCm38)	missense	probably damaging	1.00
IGL02623:Tns3	APN	11	8,437,141 (GRCm38)	missense	probably damaging	1.00
IGL02697:Tns3	APN	11	8,492,346 (GRCm38)	missense	probably benign	0.00
IGL02829:Tns3	APN	11	8,519,564 (GRCm38)	missense	probably damaging	1.00
ANU74:Tns3	UTSW	11	8,492,149 (GRCm38)	missense	probably benign	0.38
R0020:Tns3	UTSW	11	8,545,227 (GRCm38)	critical splice donor site	probably null
R0064:Tns3	UTSW	11	8,435,856 (GRCm38)	nonsense	probably null
R0064:Tns3	UTSW	11	8,435,856 (GRCm38)	nonsense	probably null
R0370:Tns3	UTSW	11	8,445,730 (GRCm38)	missense	possibly damaging	0.80
R0388:Tns3	UTSW	11	8,445,703 (GRCm38)	missense	probably benign	0.07
R0410:Tns3	UTSW	11	8,435,852 (GRCm38)	missense	probably benign	0.02
R0496:Tns3	UTSW	11	8,547,262 (GRCm38)	splice site	probably benign
R0562:Tns3	UTSW	11	8,493,262 (GRCm38)	missense	possibly damaging	0.93
R0626:Tns3	UTSW	11	8,493,121 (GRCm38)	missense	probably benign	0.04
R0736:Tns3	UTSW	11	8,519,474 (GRCm38)	missense	possibly damaging	0.94
R0893:Tns3	UTSW	11	8,493,302 (GRCm38)	missense	probably damaging	1.00
R1367:Tns3	UTSW	11	8,448,704 (GRCm38)	missense	probably benign	0.01
R1386:Tns3	UTSW	11	8,518,261 (GRCm38)	missense	probably benign	0.02
R1975:Tns3	UTSW	11	8,435,738 (GRCm38)	missense	probably benign	0.04
R2205:Tns3	UTSW	11	8,531,719 (GRCm38)	missense	probably damaging	1.00
R2319:Tns3	UTSW	11	8,541,200 (GRCm38)	missense	probably damaging	1.00
R2830:Tns3	UTSW	11	8,435,870 (GRCm38)	missense	probably damaging	1.00
R3720:Tns3	UTSW	11	8,492,999 (GRCm38)	missense	probably damaging	1.00
R3765:Tns3	UTSW	11	8,451,133 (GRCm38)	missense	probably benign	0.00
R3817:Tns3	UTSW	11	8,434,619 (GRCm38)	missense	probably damaging	1.00
R4058:Tns3	UTSW	11	8,492,275 (GRCm38)	missense	probably damaging	1.00
R4599:Tns3	UTSW	11	8,531,747 (GRCm38)	missense	probably damaging	1.00
R4631:Tns3	UTSW	11	8,451,119 (GRCm38)	missense	probably benign	0.30
R4731:Tns3	UTSW	11	8,450,986 (GRCm38)	missense	probably benign	0.28
R4732:Tns3	UTSW	11	8,450,986 (GRCm38)	missense	probably benign	0.28
R4733:Tns3	UTSW	11	8,450,986 (GRCm38)	missense	probably benign	0.28
R5472:Tns3	UTSW	11	8,451,092 (GRCm38)	missense	probably benign
R5749:Tns3	UTSW	11	8,451,177 (GRCm38)	missense	probably benign	0.01
R5807:Tns3	UTSW	11	8,493,211 (GRCm38)	missense	probably damaging	1.00
R5844:Tns3	UTSW	11	8,434,580 (GRCm38)	missense	probably damaging	1.00
R5942:Tns3	UTSW	11	8,435,860 (GRCm38)	missense	probably damaging	1.00
R5982:Tns3	UTSW	11	8,492,245 (GRCm38)	missense	probably damaging	0.99
R6025:Tns3	UTSW	11	8,492,578 (GRCm38)	missense	possibly damaging	0.93
R6266:Tns3	UTSW	11	8,492,987 (GRCm38)	missense	probably damaging	1.00
R6322:Tns3	UTSW	11	8,492,147 (GRCm38)	missense	probably benign	0.01
R6577:Tns3	UTSW	11	8,549,058 (GRCm38)	missense	probably damaging	1.00
R6577:Tns3	UTSW	11	8,549,057 (GRCm38)	missense	probably damaging	1.00
R6864:Tns3	UTSW	11	8,493,196 (GRCm38)	missense	probably damaging	1.00
R6897:Tns3	UTSW	11	8,531,743 (GRCm38)	missense	probably damaging	1.00
R7108:Tns3	UTSW	11	8,437,251 (GRCm38)	missense	probably benign	0.00
R7443:Tns3	UTSW	11	8,451,442 (GRCm38)	missense	probably benign	0.01
R7459:Tns3	UTSW	11	8,492,793 (GRCm38)	missense	probably benign	0.16
R7474:Tns3	UTSW	11	8,530,894 (GRCm38)	missense	probably damaging	1.00
R7576:Tns3	UTSW	11	8,541,192 (GRCm38)	missense	possibly damaging	0.78
R7979:Tns3	UTSW	11	8,492,701 (GRCm38)	missense	probably benign	0.01
R8055:Tns3	UTSW	11	8,545,343 (GRCm38)	missense	probably damaging	1.00
R8057:Tns3	UTSW	11	8,492,773 (GRCm38)	missense	probably benign
R8077:Tns3	UTSW	11	8,445,667 (GRCm38)	missense	probably damaging	1.00
R8518:Tns3	UTSW	11	8,492,971 (GRCm38)	missense	probably damaging	0.96
R8523:Tns3	UTSW	11	8,448,779 (GRCm38)	missense	probably damaging	1.00
R8790:Tns3	UTSW	11	8,518,273 (GRCm38)	missense	probably damaging	0.99
R9228:Tns3	UTSW	11	8,450,094 (GRCm38)	missense	probably damaging	1.00
R9374:Tns3	UTSW	11	8,492,606 (GRCm38)	missense	probably damaging	1.00
R9476:Tns3	UTSW	11	8,445,702 (GRCm38)	missense	probably damaging	0.99
R9510:Tns3	UTSW	11	8,445,702 (GRCm38)	missense	probably damaging	0.99
R9594:Tns3	UTSW	11	8,451,142 (GRCm38)	missense	possibly damaging	0.79
R9595:Tns3	UTSW	11	8,451,142 (GRCm38)	missense	possibly damaging	0.79
R9596:Tns3	UTSW	11	8,451,142 (GRCm38)	missense	possibly damaging	0.79
R9624:Tns3	UTSW	11	8,451,142 (GRCm38)	missense	possibly damaging	0.79
R9629:Tns3	UTSW	11	8,451,142 (GRCm38)	missense	possibly damaging	0.79
T0975:Tns3	UTSW	11	8,451,146 (GRCm38)	missense	probably benign	0.00
T0975:Tns3	UTSW	11	8,549,100 (GRCm38)	start gained	probably benign
T0975:Tns3	UTSW	11	8,479,518 (GRCm38)	missense	probably benign
X0005:Tns3	UTSW	11	8,479,518 (GRCm38)	missense	probably benign
X0005:Tns3	UTSW	11	8,451,224 (GRCm38)	missense	probably benign	0.00
Z1177:Tns3	UTSW	11	8,451,014 (GRCm38)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AGCAGCTTCTTTAAGGCCAG -3'
(R):5'- CCCAGGTTGCTATCATGAGGAAG -3'

Sequencing Primer
(F):5'- CAGCTTCTTTAAGGCCAGTCAGTG -3'
(R):5'- GAGAAAGCCTGCATGCATG -3'

Posted On

2018-06-06