Incidental Mutation 'R6543:Trim66'
ID 520934
Institutional Source Beutler Lab
Gene Symbol Trim66
Ensembl Gene ENSMUSG00000031026
Gene Name tripartite motif-containing 66
Synonyms Tif1d, D7H11orf29
MMRRC Submission 044669-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.226) question?
Stock # R6543 (G1)
Quality Score 225.009
Status Validated
Chromosome 7
Chromosomal Location 109449006-109508134 bp(-) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) A to G at 109475879 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Serine to Proline at position 392 (S392P)
Ref Sequence ENSEMBL: ENSMUSP00000102352 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000033339] [ENSMUST00000106739] [ENSMUST00000106741]
AlphaFold Q924W6
Predicted Effect probably benign
Transcript: ENSMUST00000033339
AA Change: S290P

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)
SMART Domains Protein: ENSMUSP00000033339
Gene: ENSMUSG00000031026
AA Change: S290P

DomainStartEndE-ValueType
PHD 4 69 7.77e0 SMART
BBC 108 234 1.61e-39 SMART
low complexity region 318 333 N/A INTRINSIC
low complexity region 452 486 N/A INTRINSIC
low complexity region 517 530 N/A INTRINSIC
low complexity region 568 581 N/A INTRINSIC
PHD 998 1041 4.09e-10 SMART
BROMO 1069 1175 8.22e-27 SMART
low complexity region 1185 1199 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000106739
AA Change: S290P

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)
SMART Domains Protein: ENSMUSP00000102350
Gene: ENSMUSG00000031026
AA Change: S290P

DomainStartEndE-ValueType
PHD 4 69 7.77e0 SMART
BBC 108 234 1.61e-39 SMART
low complexity region 318 333 N/A INTRINSIC
low complexity region 452 486 N/A INTRINSIC
low complexity region 517 530 N/A INTRINSIC
low complexity region 568 581 N/A INTRINSIC
PHD 998 1041 4.09e-10 SMART
BROMO 1069 1175 8.22e-27 SMART
low complexity region 1185 1199 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000106741
AA Change: S392P

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)
SMART Domains Protein: ENSMUSP00000102352
Gene: ENSMUSG00000031026
AA Change: S392P

DomainStartEndE-ValueType
RING 28 78 2.38e-2 SMART
BBOX 102 140 1.48e0 SMART
PHD 106 171 7.77e0 SMART
RING 107 170 4.38e0 SMART
BBOX 162 203 4.21e-3 SMART
BBC 210 336 1.61e-39 SMART
low complexity region 420 435 N/A INTRINSIC
low complexity region 554 588 N/A INTRINSIC
low complexity region 619 632 N/A INTRINSIC
low complexity region 670 683 N/A INTRINSIC
PHD 1100 1143 4.09e-10 SMART
BROMO 1171 1277 8.22e-27 SMART
low complexity region 1287 1301 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000137704
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.3%
  • 20x: 97.6%
Validation Efficiency 100% (41/41)
Allele List at MGI
Other mutations in this stock
Total: 42 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ap4e1 T C 2: 127,066,605 V1091A probably benign Het
Arhgef11 A G 3: 87,733,408 N1320D probably benign Het
Atl3 T A 19: 7,510,098 F126Y probably damaging Het
Borcs5 C T 6: 134,710,180 T167M probably damaging Het
Creld2 A G 15: 88,825,278 T331A probably benign Het
Cuzd1 A G 7: 131,309,768 V494A probably damaging Het
Dclk1 C T 3: 55,500,131 P244S probably damaging Het
Esyt3 T C 9: 99,338,772 N126S possibly damaging Het
Focad T C 4: 88,279,256 S629P unknown Het
Gm11236 A G 4: 73,638,978 E16G probably damaging Het
Gm17660 T C 5: 104,074,875 probably benign Het
Grip1 T C 10: 119,985,594 L389P probably benign Het
Igkv4-91 A T 6: 68,768,598 D105E probably damaging Het
Il20ra G A 10: 19,749,323 A117T probably damaging Het
Il6ra A G 3: 89,876,863 V367A probably damaging Het
Itga8 C T 2: 12,301,644 V47M probably damaging Het
Limk2 T C 11: 3,350,682 Y220C probably damaging Het
Lims1 T A 10: 58,412,451 C243* probably null Het
Man1a C A 10: 53,934,981 G435* probably null Het
Mtor T A 4: 148,545,596 I2250N probably damaging Het
N4bp1 G A 8: 86,861,906 Q135* probably null Het
Nbeal2 A T 9: 110,644,458 D76E probably benign Het
Nbn T C 4: 15,986,605 S669P probably benign Het
Nfrkb C T 9: 31,400,985 Q456* probably null Het
Olfr803 C A 10: 129,691,990 G17V probably benign Het
Pcnx3 C T 19: 5,665,247 A1557T probably benign Het
Pgd C T 4: 149,160,752 probably null Het
Prokr2 T A 2: 132,373,899 Q53L probably benign Het
Pxmp4 C A 2: 154,588,059 A137S possibly damaging Het
Rasef C T 4: 73,780,519 probably benign Het
Rsg1 T G 4: 141,217,288 V50G probably benign Het
Scrt2 C T 2: 152,093,143 A72V probably benign Het
Sipa1l2 T C 8: 125,450,362 E1171G possibly damaging Het
Slc26a8 T C 17: 28,638,401 T923A possibly damaging Het
Slco1a6 T C 6: 142,133,146 K141R probably benign Het
Slfn5 T A 11: 82,958,666 probably null Het
Sympk A T 7: 19,036,830 H282L probably damaging Het
Tnr A G 1: 159,924,107 Y1324C probably damaging Het
Topaz1 A G 9: 122,748,535 N170S possibly damaging Het
Trav6-3 T C 14: 53,428,762 M1T probably null Het
Ttn T C 2: 76,768,811 T10958A probably damaging Het
Ubr7 A G 12: 102,768,235 K256R probably benign Het
Other mutations in Trim66
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01539:Trim66 APN 7 109,455,066 (GRCm38) missense probably benign 0.02
IGL01758:Trim66 APN 7 109,486,045 (GRCm38) critical splice donor site probably null
IGL01982:Trim66 APN 7 109,458,763 (GRCm38) missense probably benign 0.00
IGL01983:Trim66 APN 7 109,458,251 (GRCm38) nonsense probably null
IGL02149:Trim66 APN 7 109,460,902 (GRCm38) missense possibly damaging 0.66
IGL02392:Trim66 APN 7 109,460,274 (GRCm38) missense probably benign 0.01
IGL02483:Trim66 APN 7 109,477,630 (GRCm38) splice site probably benign
IGL02832:Trim66 APN 7 109,460,497 (GRCm38) missense probably damaging 1.00
IGL02945:Trim66 APN 7 109,460,176 (GRCm38) nonsense probably null
IGL03085:Trim66 APN 7 109,458,745 (GRCm38) missense probably benign 0.17
PIT1430001:Trim66 UTSW 7 109,475,247 (GRCm38) missense probably damaging 0.99
R0326:Trim66 UTSW 7 109,460,172 (GRCm38) missense probably benign 0.00
R0358:Trim66 UTSW 7 109,460,176 (GRCm38) nonsense probably null
R0401:Trim66 UTSW 7 109,475,264 (GRCm38) missense probably damaging 0.98
R0470:Trim66 UTSW 7 109,457,542 (GRCm38) splice site probably benign
R0568:Trim66 UTSW 7 109,460,695 (GRCm38) missense probably benign 0.00
R0669:Trim66 UTSW 7 109,454,992 (GRCm38) intron probably benign
R0980:Trim66 UTSW 7 109,455,670 (GRCm38) missense probably damaging 1.00
R1015:Trim66 UTSW 7 109,455,233 (GRCm38) missense probably damaging 1.00
R1078:Trim66 UTSW 7 109,472,319 (GRCm38) missense probably damaging 1.00
R1099:Trim66 UTSW 7 109,475,454 (GRCm38) missense probably benign 0.34
R1181:Trim66 UTSW 7 109,484,577 (GRCm38) critical splice donor site probably null
R1497:Trim66 UTSW 7 109,484,619 (GRCm38) missense probably benign 0.00
R1583:Trim66 UTSW 7 109,455,080 (GRCm38) missense probably damaging 1.00
R1843:Trim66 UTSW 7 109,475,839 (GRCm38) missense probably damaging 0.99
R1998:Trim66 UTSW 7 109,484,577 (GRCm38) critical splice donor site probably null
R2016:Trim66 UTSW 7 109,472,232 (GRCm38) critical splice donor site probably null
R2143:Trim66 UTSW 7 109,475,113 (GRCm38) missense probably damaging 0.98
R2144:Trim66 UTSW 7 109,475,113 (GRCm38) missense probably damaging 0.98
R2145:Trim66 UTSW 7 109,475,113 (GRCm38) missense probably damaging 0.98
R3945:Trim66 UTSW 7 109,472,268 (GRCm38) missense possibly damaging 0.94
R4012:Trim66 UTSW 7 109,458,131 (GRCm38) missense probably damaging 0.98
R4464:Trim66 UTSW 7 109,477,690 (GRCm38) missense possibly damaging 0.51
R4473:Trim66 UTSW 7 109,481,995 (GRCm38) missense probably damaging 1.00
R4729:Trim66 UTSW 7 109,456,060 (GRCm38) critical splice donor site probably null
R4730:Trim66 UTSW 7 109,483,069 (GRCm38) missense probably damaging 1.00
R4775:Trim66 UTSW 7 109,457,589 (GRCm38) nonsense probably null
R4819:Trim66 UTSW 7 109,457,586 (GRCm38) missense probably damaging 1.00
R5269:Trim66 UTSW 7 109,457,590 (GRCm38) missense probably benign 0.00
R5557:Trim66 UTSW 7 109,483,737 (GRCm38) missense probably benign 0.06
R5832:Trim66 UTSW 7 109,455,202 (GRCm38) missense probably damaging 1.00
R6220:Trim66 UTSW 7 109,483,093 (GRCm38) missense probably damaging 0.97
R6243:Trim66 UTSW 7 109,460,274 (GRCm38) missense probably benign 0.01
R6374:Trim66 UTSW 7 109,486,062 (GRCm38) missense probably benign
R6450:Trim66 UTSW 7 109,460,738 (GRCm38) missense probably benign 0.09
R6788:Trim66 UTSW 7 109,477,754 (GRCm38) missense probably damaging 1.00
R6842:Trim66 UTSW 7 109,460,776 (GRCm38) missense probably benign 0.00
R7169:Trim66 UTSW 7 109,455,121 (GRCm38) missense probably benign 0.25
R7257:Trim66 UTSW 7 109,460,244 (GRCm38) missense probably damaging 1.00
R7328:Trim66 UTSW 7 109,457,751 (GRCm38) missense probably damaging 0.99
R7616:Trim66 UTSW 7 109,483,749 (GRCm38) missense probably damaging 0.99
R8423:Trim66 UTSW 7 109,475,392 (GRCm38) missense possibly damaging 0.77
R8855:Trim66 UTSW 7 109,481,981 (GRCm38) missense probably damaging 1.00
R9130:Trim66 UTSW 7 109,477,689 (GRCm38) missense possibly damaging 0.90
R9137:Trim66 UTSW 7 109,475,123 (GRCm38) missense probably damaging 0.99
R9640:Trim66 UTSW 7 109,475,618 (GRCm38) missense probably damaging 1.00
RF013:Trim66 UTSW 7 109,460,753 (GRCm38) missense probably damaging 0.99
RF024:Trim66 UTSW 7 109,460,740 (GRCm38) missense possibly damaging 0.62
Predicted Primers PCR Primer
(F):5'- ACCATTTCAGAAGGCTGTCGG -3'
(R):5'- GCTCCTGCAAATATCCCTGGAAG -3'

Sequencing Primer
(F):5'- TGTCGGAAACTGTGAGCC -3'
(R):5'- GCAAATATCCCTGGAAGCCTTGTTG -3'
Posted On 2018-06-06