Incidental Mutation 'R6544:Zfp777'
ID 521015
Institutional Source Beutler Lab
Gene Symbol Zfp777
Ensembl Gene ENSMUSG00000071477
Gene Name zinc finger protein 777
Synonyms 2500002G23Rik
MMRRC Submission 044670-MU
Accession Numbers
Essential gene? Probably essential (E-score: 0.837) question?
Stock # R6544 (G1)
Quality Score 225.009
Status Validated
Chromosome 6
Chromosomal Location 48001122-48025845 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 48021419 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Serine to Threonine at position 68 (S68T)
Ref Sequence ENSEMBL: ENSMUSP00000117227 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000095944] [ENSMUST00000114583] [ENSMUST00000125385] [ENSMUST00000147281]
AlphaFold B9EKF4
Predicted Effect probably damaging
Transcript: ENSMUST00000095944
AA Change: S68T

PolyPhen 2 Score 0.976 (Sensitivity: 0.76; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000093637
Gene: ENSMUSG00000071477
AA Change: S68T

DomainStartEndE-ValueType
Pfam:DUF3669 177 256 4.3e-12 PFAM
KRAB 284 344 1.6e-29 SMART
low complexity region 422 433 N/A INTRINSIC
coiled coil region 454 477 N/A INTRINSIC
low complexity region 489 505 N/A INTRINSIC
low complexity region 520 532 N/A INTRINSIC
low complexity region 539 555 N/A INTRINSIC
ZnF_C2H2 557 579 1.2e-5 SMART
ZnF_C2H2 585 607 3.8e-5 SMART
ZnF_C2H2 646 668 1.1e-6 SMART
ZnF_C2H2 674 696 4.6e-6 SMART
ZnF_C2H2 704 726 3.2e-7 SMART
ZnF_C2H2 732 754 3.3e-6 SMART
ZnF_C2H2 760 782 8.4e-6 SMART
ZnF_C2H2 788 810 4.9e-5 SMART
ZnF_C2H2 816 838 1.1e-4 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000114583
AA Change: S112T

PolyPhen 2 Score 0.976 (Sensitivity: 0.76; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000110230
Gene: ENSMUSG00000071477
AA Change: S112T

DomainStartEndE-ValueType
low complexity region 2 24 N/A INTRINSIC
Pfam:DUF3669 231 298 4.7e-12 PFAM
KRAB 328 388 3.96e-27 SMART
low complexity region 466 477 N/A INTRINSIC
coiled coil region 498 521 N/A INTRINSIC
low complexity region 533 549 N/A INTRINSIC
low complexity region 564 576 N/A INTRINSIC
low complexity region 583 599 N/A INTRINSIC
ZnF_C2H2 601 623 2.95e-3 SMART
ZnF_C2H2 629 651 8.94e-3 SMART
ZnF_C2H2 690 712 2.43e-4 SMART
ZnF_C2H2 718 740 1.12e-3 SMART
ZnF_C2H2 748 770 7.49e-5 SMART
ZnF_C2H2 776 798 7.9e-4 SMART
ZnF_C2H2 804 826 1.95e-3 SMART
ZnF_C2H2 832 854 1.18e-2 SMART
ZnF_C2H2 860 882 2.53e-2 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000125385
AA Change: S68T

PolyPhen 2 Score 0.271 (Sensitivity: 0.91; Specificity: 0.88)
Predicted Effect probably damaging
Transcript: ENSMUST00000147281
AA Change: S68T

PolyPhen 2 Score 0.976 (Sensitivity: 0.76; Specificity: 0.96)
Predicted Effect probably benign
Transcript: ENSMUST00000148362
SMART Domains Protein: ENSMUSP00000116303
Gene: ENSMUSG00000071477

DomainStartEndE-ValueType
Pfam:DUF3669 40 107 6.1e-13 PFAM
KRAB 137 197 3.96e-27 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000175187
Predicted Effect noncoding transcript
Transcript: ENSMUST00000203645
Meta Mutation Damage Score 0.0629 question?
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.4%
  • 20x: 97.9%
Validation Efficiency 100% (48/48)
Allele List at MGI
Other mutations in this stock
Total: 48 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
A030005K14Rik T A 1: 83,036,678 (GRCm39) K118* probably null Het
Actr2 A G 11: 20,050,933 (GRCm39) F17L probably damaging Het
Adam26b T C 8: 43,974,818 (GRCm39) I61M probably damaging Het
Ahcyl A C 16: 45,975,147 (GRCm39) S77A probably benign Het
Ahnak2 A G 12: 112,746,829 (GRCm39) probably benign Het
Angptl3 T C 4: 98,919,675 (GRCm39) L145P probably damaging Het
Ank2 T C 3: 126,726,871 (GRCm39) T808A probably damaging Het
Ark2c T A 18: 77,650,931 (GRCm39) probably benign Het
Cadm3 A G 1: 173,194,977 (GRCm39) probably null Het
Cog7 C T 7: 121,534,966 (GRCm39) R573Q probably damaging Het
Dchs1 T A 7: 105,407,385 (GRCm39) I2110F probably damaging Het
Fbxo47 G A 11: 97,747,089 (GRCm39) R326C probably damaging Het
Frmpd1 A T 4: 45,279,024 (GRCm39) D583V probably damaging Het
Gigyf1 T A 5: 137,523,321 (GRCm39) L911Q probably damaging Het
Gprin1 G A 13: 54,888,124 (GRCm39) A50V possibly damaging Het
Grik4 A T 9: 42,459,024 (GRCm39) Y571* probably null Het
Gucy2e A G 11: 69,126,483 (GRCm39) V299A probably benign Het
Hectd2 C T 19: 36,589,728 (GRCm39) L618F probably damaging Het
Lactbl1 A T 4: 136,360,300 (GRCm39) I160F possibly damaging Het
Lats1 T A 10: 7,577,434 (GRCm39) V186D possibly damaging Het
Lmtk2 A G 5: 144,110,624 (GRCm39) H448R possibly damaging Het
Map10 T C 8: 126,398,113 (GRCm39) I502T probably benign Het
Mok A G 12: 110,777,189 (GRCm39) F239S probably damaging Het
Mprip G A 11: 59,648,552 (GRCm39) G752D probably benign Het
Naip5 C A 13: 100,359,652 (GRCm39) G528V possibly damaging Het
Neu2 T C 1: 87,524,464 (GRCm39) W150R probably damaging Het
Or1ak2 T A 2: 36,827,539 (GRCm39) M136K possibly damaging Het
Or5an1 T C 19: 12,260,519 (GRCm39) Y36H probably damaging Het
Or5as1 T A 2: 86,980,207 (GRCm39) Y266F probably damaging Het
Or5l13 T A 2: 87,780,335 (GRCm39) M81L probably benign Het
Pip5k1c T A 10: 81,144,830 (GRCm39) Y224N probably damaging Het
Plch1 T C 3: 63,758,399 (GRCm39) E5G probably damaging Het
Pspc1 T C 14: 57,001,660 (GRCm39) *59W probably null Het
Ptprq T C 10: 107,444,102 (GRCm39) T1501A probably damaging Het
Rorb G T 19: 18,929,614 (GRCm39) P304T possibly damaging Het
Scn7a A T 2: 66,514,444 (GRCm39) L1110Q probably damaging Het
Serpine2 C T 1: 79,780,847 (GRCm39) probably null Het
Slco1c1 A G 6: 141,477,170 (GRCm39) probably null Het
Smarca2 T A 19: 26,608,331 (GRCm39) V130D probably damaging Het
Sox17 G T 1: 4,562,655 (GRCm39) P117T possibly damaging Het
Sparcl1 A T 5: 104,240,310 (GRCm39) Y371* probably null Het
Tdpoz2 T C 3: 93,559,267 (GRCm39) D235G possibly damaging Het
Tns2 A C 15: 102,022,269 (GRCm39) K1182N possibly damaging Het
Tpte G T 8: 22,805,121 (GRCm39) probably null Het
Ttn A T 2: 76,799,503 (GRCm39) I459K possibly damaging Het
Wdr97 T C 15: 76,242,478 (GRCm39) Y823H probably benign Het
Zc3h15 G A 2: 83,491,492 (GRCm39) R240H probably benign Het
Zfp455 C A 13: 67,355,121 (GRCm39) L130I probably benign Het
Other mutations in Zfp777
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01530:Zfp777 APN 6 48,020,918 (GRCm39) missense probably damaging 1.00
IGL01916:Zfp777 APN 6 48,002,276 (GRCm39) missense probably damaging 1.00
IGL01959:Zfp777 APN 6 48,021,275 (GRCm39) missense probably benign
IGL02167:Zfp777 APN 6 48,021,460 (GRCm39) missense probably damaging 0.98
IGL03150:Zfp777 APN 6 48,021,059 (GRCm39) missense probably damaging 1.00
R0238:Zfp777 UTSW 6 48,001,903 (GRCm39) missense probably damaging 0.99
R0238:Zfp777 UTSW 6 48,001,903 (GRCm39) missense probably damaging 0.99
R0372:Zfp777 UTSW 6 48,021,410 (GRCm39) missense possibly damaging 0.62
R0762:Zfp777 UTSW 6 48,006,294 (GRCm39) missense probably damaging 1.00
R1300:Zfp777 UTSW 6 48,002,704 (GRCm39) missense probably benign 0.43
R1727:Zfp777 UTSW 6 48,020,824 (GRCm39) missense probably damaging 0.99
R1906:Zfp777 UTSW 6 48,018,995 (GRCm39) missense probably damaging 0.99
R2047:Zfp777 UTSW 6 48,021,280 (GRCm39) missense probably benign
R2097:Zfp777 UTSW 6 48,021,176 (GRCm39) missense probably benign 0.08
R2211:Zfp777 UTSW 6 48,020,819 (GRCm39) missense possibly damaging 0.79
R2898:Zfp777 UTSW 6 48,002,594 (GRCm39) missense probably damaging 0.97
R3123:Zfp777 UTSW 6 48,006,050 (GRCm39) unclassified probably benign
R3832:Zfp777 UTSW 6 48,021,149 (GRCm39) missense probably benign 0.00
R4019:Zfp777 UTSW 6 48,019,046 (GRCm39) missense probably damaging 1.00
R4077:Zfp777 UTSW 6 48,002,456 (GRCm39) missense probably benign
R4471:Zfp777 UTSW 6 48,019,041 (GRCm39) missense probably damaging 1.00
R5021:Zfp777 UTSW 6 48,019,061 (GRCm39) missense probably damaging 0.99
R5030:Zfp777 UTSW 6 48,014,601 (GRCm39) missense probably damaging 0.99
R5819:Zfp777 UTSW 6 48,014,522 (GRCm39) missense probably damaging 0.99
R6736:Zfp777 UTSW 6 48,001,790 (GRCm39) missense probably damaging 0.99
R6971:Zfp777 UTSW 6 48,001,625 (GRCm39) missense probably damaging 1.00
R7240:Zfp777 UTSW 6 48,021,383 (GRCm39) missense probably benign 0.00
R7258:Zfp777 UTSW 6 48,002,731 (GRCm39) missense probably damaging 0.99
R7586:Zfp777 UTSW 6 48,006,152 (GRCm39) missense probably benign 0.33
R7833:Zfp777 UTSW 6 48,002,072 (GRCm39) missense probably damaging 0.99
R7947:Zfp777 UTSW 6 48,001,645 (GRCm39) missense probably damaging 1.00
R8136:Zfp777 UTSW 6 48,021,559 (GRCm39) missense probably benign 0.25
R8151:Zfp777 UTSW 6 48,006,075 (GRCm39) nonsense probably null
R8348:Zfp777 UTSW 6 48,006,101 (GRCm39) missense probably damaging 0.99
R8448:Zfp777 UTSW 6 48,006,101 (GRCm39) missense probably damaging 0.99
R8942:Zfp777 UTSW 6 48,006,125 (GRCm39) missense probably benign 0.25
R8983:Zfp777 UTSW 6 48,006,158 (GRCm39) missense probably damaging 1.00
R9205:Zfp777 UTSW 6 48,002,521 (GRCm39) missense probably benign 0.07
R9397:Zfp777 UTSW 6 48,021,190 (GRCm39) missense probably benign 0.00
R9562:Zfp777 UTSW 6 48,021,580 (GRCm39) missense possibly damaging 0.84
R9565:Zfp777 UTSW 6 48,021,580 (GRCm39) missense possibly damaging 0.84
RF008:Zfp777 UTSW 6 48,018,982 (GRCm39) nonsense probably null
Z1177:Zfp777 UTSW 6 48,002,168 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TCTCAGGAACCTCAGGAGAGTG -3'
(R):5'- TTGAACACAGGTCCAGCAGC -3'

Sequencing Primer
(F):5'- AGTGGAGCTTCCTGACGG -3'
(R):5'- ACATGGAGGACGATCGCTC -3'
Posted On 2018-06-06