Mutagenetix > Incidental Mutation

Incidental Mutation 'R6519:Zfp955b'

521048

Institutional Source

Beutler Lab

Gene Symbol

Zfp955b

Ensembl Gene

ENSMUSG00000096910

Gene Name

zinc finger protein 955B

Synonyms

A430003O12Rik, C430039G02Rik, Gm4455

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.083) question?

Stock #

R6519 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

33289649-33303196 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 33302077 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Arginine at position 173 (S173R)

Ref Sequence

ENSEMBL: ENSMUSP00000097011 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099414]

AlphaFold

L7N232

Predicted Effect

possibly damaging
Transcript: ENSMUST00000099414
AA Change: S173R

PolyPhen 2 Score 0.546 (Sensitivity: 0.88; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000097011
Gene: ENSMUSG00000096910
AA Change: S173R

Domain	Start	End	E-Value	Type
KRAB	10	70	3.04e-14	SMART
ZnF_C2H2	230	252	7.68e0	SMART
ZnF_C2H2	258	280	5.72e-1	SMART
ZnF_C2H2	290	312	6.75e0	SMART
ZnF_C2H2	318	340	5.81e-2	SMART
ZnF_C2H2	346	368	3.16e-3	SMART
ZnF_C2H2	374	396	1.18e-2	SMART
ZnF_C2H2	402	424	7.78e-3	SMART
ZnF_C2H2	430	452	3.16e-3	SMART
ZnF_C2H2	458	480	1.1e-2	SMART
ZnF_C2H2	486	508	2.09e-3	SMART
ZnF_C2H2	514	536	6.67e-2	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000182230

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.5%
20x: 91.7%

Validation Efficiency

97% (58/60)

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A4gnt	T	C	9: 99,613,670	I53T	probably damaging	Het
Adgrv1	C	T	13: 81,567,343	D909N	probably benign	Het
Ahdc1	T	C	4: 133,064,768	Y1107H	possibly damaging	Het
Aldob	A	T	4: 49,543,835	V49E	probably damaging	Het
Apol6	T	A	15: 77,051,276	Y248*	probably null	Het
Apol7b	T	A	15: 77,423,348	T316S	probably benign	Het
Atp13a2	G	C	4: 141,000,854	R503P	possibly damaging	Het
BC037034	A	G	5: 138,261,848	S344P	probably damaging	Het
Brca2	A	C	5: 150,540,979	T1403P	probably damaging	Het
Casc4	T	C	2: 121,906,737	V141A	probably benign	Het
Cblc	T	C	7: 19,792,863	Y148C	probably damaging	Het
Cct7	C	A	6: 85,462,150	Q149K	probably benign	Het
Cd53	T	A	3: 106,762,145	H179L	probably benign	Het
Cyp2b19	A	G	7: 26,759,111	T84A	probably benign	Het
Cyp3a41a	A	G	5: 145,715,498	C64R	probably damaging	Het
Dclre1c	T	C	2: 3,429,329	Y75H	probably damaging	Het
Dhx35	A	T	2: 158,831,710	I354F	probably damaging	Het
Diaph3	T	C	14: 86,966,335	N629S	probably damaging	Het
Dnase1	A	T	16: 4,038,589	S132C	probably damaging	Het
Dnttip2	T	C	3: 122,275,471	S112P	probably benign	Het
Eif4g3	C	A	4: 137,994,008	P48T	probably benign	Het
Fat4	A	T	3: 39,002,871	T4239S	probably benign	Het
Fbn2	A	G	18: 58,063,575	V1419A	possibly damaging	Het
Ghitm	A	C	14: 37,125,247	M290R	probably damaging	Het
Glb1l	T	C	1: 75,201,056	D406G	probably benign	Het
Glipr1l1	C	A	10: 112,062,248	A86D	probably benign	Het
Grm7	C	T	6: 111,207,752	A348V	probably benign	Het
Gtf2h3	C	T	5: 124,584,297	T121I	probably benign	Het
Hdac2	T	A	10: 36,989,256	N155K	probably damaging	Het
Hus1b	A	G	13: 30,946,947	I243T	probably benign	Het
Kcnab2	T	C	4: 152,411,993	T65A	probably damaging	Het
Lasp1	T	A	11: 97,815,557		probably null	Het
Lrch3	G	A	16: 32,994,997		probably benign	Het
Ltb4r2	C	T	14: 55,762,981	T353M	probably benign	Het
Macf1	A	G	4: 123,472,325	M1316T	probably benign	Het
Msr1	G	A	8: 39,624,221	T116I	probably benign	Het
Nlrp5	A	G	7: 23,417,918	I356V	probably benign	Het
Npy	C	T	6: 49,823,689	S31F	possibly damaging	Het
Nsd3	C	T	8: 25,662,939	P432S	probably damaging	Het
Nup160	A	C	2: 90,718,217	R1037S	probably damaging	Het
Olfr284	C	T	15: 98,340,048	G314R	probably benign	Het
Olfr32	A	T	2: 90,138,812	I109N	possibly damaging	Het
Olfr533	T	A	7: 140,466,545	S115T	probably benign	Het
Pcx	A	G	19: 4,602,211	E108G	possibly damaging	Het
Pecam1	A	T	11: 106,699,642	M102K	probably benign	Het
Pgd	G	T	4: 149,150,886	Y433*	probably null	Het
Pkd1l3	A	G	8: 109,628,772	E744G	probably benign	Het
Rb1	A	G	14: 73,298,063	I118T	probably benign	Het
Rdh11	T	A	12: 79,182,815	H228L	probably damaging	Het
Rnf44	C	T	13: 54,681,786	R340Q	probably damaging	Het
Rtraf	A	G	14: 19,819,930	V88A	possibly damaging	Het
Sigmar1	T	C	4: 41,739,380	T185A	possibly damaging	Het
Thsd1	A	G	8: 22,259,065	R590G	probably damaging	Het
Trbv19	T	C	6: 41,178,639		probably benign	Het
Txnrd3	T	C	6: 89,654,423		probably null	Het
Wwc1	C	T	11: 35,853,437	E853K	probably benign	Het
Xpnpep1	T	C	19: 53,011,844	N192D	possibly damaging	Het
Zranb1	T	A	7: 132,950,128	C195*	probably null	Het

Other mutations in Zfp955b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00537:Zfp955b	APN	17	33302873	missense	probably damaging	1.00
IGL02073:Zfp955b	APN	17	33300590	missense	possibly damaging	0.69
IGL02126:Zfp955b	APN	17	33302264	nonsense	probably null
IGL02237:Zfp955b	APN	17	33301919	missense	probably damaging	1.00
IGL02587:Zfp955b	APN	17	33300650	missense	probably damaging	1.00
IGL02971:Zfp955b	APN	17	33300966	missense	probably benign	0.11
IGL03034:Zfp955b	APN	17	33302168	missense	probably benign	0.22
IGL03493:Zfp955b	APN	17	33302545	missense	probably benign	0.35
R0269:Zfp955b	UTSW	17	33305463	missense	probably damaging	1.00
R0373:Zfp955b	UTSW	17	33302522	missense	probably benign
R0617:Zfp955b	UTSW	17	33305463	missense	probably damaging	1.00
R0684:Zfp955b	UTSW	17	33302973	missense	probably benign	0.00
R1778:Zfp955b	UTSW	17	33302814	missense	probably benign	0.07
R1874:Zfp955b	UTSW	17	33305453	missense	probably benign	0.10
R3893:Zfp955b	UTSW	17	33302994	missense	probably benign	0.01
R3938:Zfp955b	UTSW	17	33305416	missense	probably damaging	1.00
R4082:Zfp955b	UTSW	17	33302155	missense	probably benign	0.08
R4672:Zfp955b	UTSW	17	33305259	unclassified	probably benign
R4956:Zfp955b	UTSW	17	33305235	unclassified	probably benign
R4998:Zfp955b	UTSW	17	33305151	unclassified	probably benign
R5276:Zfp955b	UTSW	17	33303057	missense	probably damaging	1.00
R5341:Zfp955b	UTSW	17	33305121	unclassified	probably benign
R5558:Zfp955b	UTSW	17	33302187	missense	possibly damaging	0.88
R6086:Zfp955b	UTSW	17	33302504	missense	probably benign
R6170:Zfp955b	UTSW	17	33302110	missense	probably benign	0.00
R6306:Zfp955b	UTSW	17	33303186	missense	probably benign	0.07
R9286:Zfp955b	UTSW	17	33302709	missense	probably benign	0.04

Predicted Primers

PCR Primer
(F):5'- TGCAGTCACGGTTTATGAAAGC -3'
(R):5'- TCCACATTGCTTATACTCAAGTGG -3'

Sequencing Primer
(F):5'- CAGTCACGGTTTATGAAAGCAGTGTG -3'
(R):5'- ACTCAAGTGGTTTTTCTCCAGTATG -3'

Posted On

2018-06-06