Mutagenetix > Incidental Mutation

Incidental Mutation 'R6553:Gpr155'

521764

Institutional Source

Beutler Lab

Gene Symbol

Gpr155

Ensembl Gene

ENSMUSG00000041762

Gene Name

G protein-coupled receptor 155

Synonyms

PGR22, 1110017O10Rik, DEPDC3, F730029F15Rik

MMRRC Submission

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6553 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

73341506-73386572 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 73349645 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 157 (I157N)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076463] [ENSMUST00000112043] [ENSMUST00000112044]

AlphaFold

A2AWR3

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000048504

SMART Domains

Protein: ENSMUSP00000037772
Gene: ENSMUSG00000041762

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	1.9e-16	PFAM
Pfam:Mem_trans	187	360	4.9e-12	PFAM
transmembrane domain	380	402	N/A	INTRINSIC
transmembrane domain	415	432	N/A	INTRINSIC
transmembrane domain	442	464	N/A	INTRINSIC
transmembrane domain	477	499	N/A	INTRINSIC
transmembrane domain	519	538	N/A	INTRINSIC
transmembrane domain	657	679	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000076463
AA Change: I717N

PolyPhen 2 Score 0.948 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000075788
Gene: ENSMUSG00000041762
AA Change: I717N

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	3.3e-15	PFAM
transmembrane domain	209	231	N/A	INTRINSIC
transmembrane domain	241	260	N/A	INTRINSIC
transmembrane domain	272	291	N/A	INTRINSIC
transmembrane domain	311	330	N/A	INTRINSIC
transmembrane domain	343	365	N/A	INTRINSIC
transmembrane domain	380	402	N/A	INTRINSIC
transmembrane domain	415	432	N/A	INTRINSIC
transmembrane domain	442	464	N/A	INTRINSIC
transmembrane domain	477	499	N/A	INTRINSIC
transmembrane domain	519	538	N/A	INTRINSIC
transmembrane domain	657	679	N/A	INTRINSIC
transmembrane domain	689	711	N/A	INTRINSIC
DEP	759	833	8.28e-12	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000112043
AA Change: I717N

PolyPhen 2 Score 0.948 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000107674
Gene: ENSMUSG00000041762
AA Change: I717N

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	3.3e-15	PFAM
transmembrane domain	209	231	N/A	INTRINSIC
transmembrane domain	241	260	N/A	INTRINSIC
transmembrane domain	272	291	N/A	INTRINSIC
transmembrane domain	311	330	N/A	INTRINSIC
transmembrane domain	343	365	N/A	INTRINSIC
transmembrane domain	380	402	N/A	INTRINSIC
transmembrane domain	415	432	N/A	INTRINSIC
transmembrane domain	442	464	N/A	INTRINSIC
transmembrane domain	477	499	N/A	INTRINSIC
transmembrane domain	519	538	N/A	INTRINSIC
transmembrane domain	657	679	N/A	INTRINSIC
transmembrane domain	689	711	N/A	INTRINSIC
DEP	759	833	8.28e-12	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000112044
AA Change: I689N

PolyPhen 2 Score 0.948 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000107675
Gene: ENSMUSG00000041762
AA Change: I689N

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	3.3e-15	PFAM
Pfam:Mem_trans	187	360	8.3e-11	PFAM
transmembrane domain	382	404	N/A	INTRINSIC
transmembrane domain	414	436	N/A	INTRINSIC
transmembrane domain	449	471	N/A	INTRINSIC
transmembrane domain	491	510	N/A	INTRINSIC
transmembrane domain	629	651	N/A	INTRINSIC
transmembrane domain	661	683	N/A	INTRINSIC
DEP	731	805	8.28e-12	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000131430
AA Change: I157N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000114294
Gene: ENSMUSG00000041762
AA Change: I157N

Domain	Start	End	E-Value	Type
transmembrane domain	98	120	N/A	INTRINSIC
transmembrane domain	130	152	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000184503

Meta Mutation Damage Score

0.5703

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.3%
20x: 97.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 26 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam1b	G	T	5: 121,501,187	D598E	probably benign	Het
Ascc3	A	G	10: 50,842,177	K1989E	probably benign	Het
Asic5	A	T	3: 82,009,466	T288S	possibly damaging	Het
Chd1	A	G	17: 15,725,430	N72S	probably benign	Het
Ciita	T	A	16: 10,511,745	V628E	probably benign	Het
Cyp2c50	A	G	19: 40,090,602	T130A	probably benign	Het
Dapk1	T	A	13: 60,761,161	V1196E	probably damaging	Het
Dis3l2	T	A	1: 86,745,494	I69N	probably damaging	Het
Exph5	A	G	9: 53,301,712		probably benign	Het
Fcgbp	A	T	7: 28,113,979	Q2313L	possibly damaging	Het
Gm2888	A	G	14: 3,037,722	H238R	possibly damaging	Het
Gm5622	A	G	14: 51,657,743	K120E	probably damaging	Het
Gm7534	A	G	4: 134,202,056	S313P	probably damaging	Het
Hltf	T	C	3: 20,072,394	V245A	probably damaging	Het
Kmt2e	G	A	5: 23,463,026	V28I	probably damaging	Het
Lsm3	GATATATA	GATATATATA	6: 91,519,635		probably null	Het
Nprl3	C	T	11: 32,234,812	R399Q	probably benign	Het
Olfr804	C	T	10: 129,705,063	R62C	probably benign	Het
Ptgs2	T	C	1: 150,103,987	V281A	possibly damaging	Het
Speer4f2	A	G	5: 17,374,422	E73G	probably damaging	Het
Tmem161b	C	A	13: 84,222,418		probably benign	Het
Trav13n-3	A	G	14: 53,337,161	T14A	probably benign	Het
Trav9d-4	A	T	14: 52,983,741	Q63L	probably benign	Het
Vmn2r75	T	A	7: 86,164,245	N450Y	probably benign	Het
Vmn2r97	G	A	17: 18,930,304	W471*	probably null	Het
Zfp27	G	A	7: 29,896,393	T49I	possibly damaging	Het

Other mutations in Gpr155

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00731:Gpr155	APN	2	73362613	missense	probably damaging	1.00
IGL01432:Gpr155	APN	2	73351885	missense	possibly damaging	0.51
IGL01528:Gpr155	APN	2	73362423	critical splice donor site	probably null
IGL01718:Gpr155	APN	2	73382232	missense	probably benign
IGL01733:Gpr155	APN	2	73353612	splice site	probably null
IGL03342:Gpr155	APN	2	73349678	missense	probably damaging	1.00
PIT1430001:Gpr155	UTSW	2	73370138	missense	probably benign	0.08
PIT4810001:Gpr155	UTSW	2	73348263	missense	probably benign	0.08
R0226:Gpr155	UTSW	2	73367592	missense	probably benign	0.02
R0399:Gpr155	UTSW	2	73370002	missense	possibly damaging	0.91
R0445:Gpr155	UTSW	2	73370144	splice site	probably benign
R1570:Gpr155	UTSW	2	73370038	missense	possibly damaging	0.87
R1598:Gpr155	UTSW	2	73370090	missense	probably damaging	0.98
R1647:Gpr155	UTSW	2	73364164	splice site	probably null
R1648:Gpr155	UTSW	2	73364164	splice site	probably null
R1756:Gpr155	UTSW	2	73367577	missense	probably benign	0.00
R1760:Gpr155	UTSW	2	73381935	missense	probably damaging	1.00
R2033:Gpr155	UTSW	2	73348182	missense	probably benign	0.18
R2044:Gpr155	UTSW	2	73373633	missense	probably damaging	1.00
R2145:Gpr155	UTSW	2	73356658	missense	probably benign	0.01
R2172:Gpr155	UTSW	2	73382127	missense	probably benign	0.00
R2274:Gpr155	UTSW	2	73348135	critical splice donor site	probably null
R3878:Gpr155	UTSW	2	73368392	nonsense	probably null
R3924:Gpr155	UTSW	2	73370076	missense	probably damaging	1.00
R4910:Gpr155	UTSW	2	73367538	nonsense	probably null
R4950:Gpr155	UTSW	2	73382185	missense	probably benign
R5337:Gpr155	UTSW	2	73348248	missense	probably benign	0.32
R5830:Gpr155	UTSW	2	73370089	missense	possibly damaging	0.93
R5887:Gpr155	UTSW	2	73343718	nonsense	probably null
R5929:Gpr155	UTSW	2	73373667	nonsense	probably null
R6293:Gpr155	UTSW	2	73373997	missense	possibly damaging	0.47
R6585:Gpr155	UTSW	2	73349645	missense	probably damaging	1.00
R7003:Gpr155	UTSW	2	73343617	missense	probably damaging	0.99
R7353:Gpr155	UTSW	2	73367491	nonsense	probably null
R7506:Gpr155	UTSW	2	73368339	missense	probably damaging	0.97
R7631:Gpr155	UTSW	2	73382947	intron	probably benign
R7753:Gpr155	UTSW	2	73382206	missense	probably benign	0.27
R7810:Gpr155	UTSW	2	73381952	missense	probably damaging	0.99
R7813:Gpr155	UTSW	2	73381985	nonsense	probably null
R7815:Gpr155	UTSW	2	73362560	missense	probably benign
R7873:Gpr155	UTSW	2	73343590	missense	possibly damaging	0.51
R8506:Gpr155	UTSW	2	73343462	missense	probably damaging	0.99
R8680:Gpr155	UTSW	2	73343695	missense	probably damaging	0.99
R8856:Gpr155	UTSW	2	73373649	missense	probably benign	0.27
R8872:Gpr155	UTSW	2	73367592	missense	probably benign	0.02
R9116:Gpr155	UTSW	2	73373765	missense	possibly damaging	0.78
R9683:Gpr155	UTSW	2	73362436	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TGCCTGTCAGCTCTTGTTAAAATG -3'
(R):5'- GTCTACCCTGGATGCTGAATTTC -3'

Sequencing Primer
(F):5'- GCTGGATTTATAATACCACTTCCG -3'
(R):5'- CTCGCTCACTAAGCATCT -3'

Posted On

2018-06-06