Mutagenetix > Incidental Mutation

Incidental Mutation 'R6526:Vmn2r44'

521848

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r44

Ensembl Gene

ENSMUSG00000094098

Gene Name

vomeronasal 2, receptor 44

Synonyms

EG434113

MMRRC Submission

044652-MU

Accession Numbers

Essential gene?

Not available question?

Stock #

R6526 (G1)

Quality Score

172.009

Status

Not validated

Chromosome

Chromosomal Location

8370459-8386237 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 8381098 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 265 (M265K)

Ref Sequence

ENSEMBL: ENSMUSP00000132467 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166499]

AlphaFold

L7N2E1

Predicted Effect

probably benign
Transcript: ENSMUST00000166499
AA Change: M265K

PolyPhen 2 Score 0.014 (Sensitivity: 0.96; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000132467
Gene: ENSMUSG00000094098
AA Change: M265K

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	73	469	2.5e-34	PFAM
Pfam:NCD3G	512	565	3.8e-20	PFAM
Pfam:7tm_3	598	833	5.7e-55	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.4%
20x: 91.5%

Validation Efficiency

100% (72/72)

Allele List at MGI

Other mutations in this stock

Total: 72 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aamp	A	G	1: 74,323,331 (GRCm39)		probably null	Het
Abcc3	C	T	11: 94,250,198 (GRCm39)	G975D	probably benign	Het
Abhd13	G	A	8: 10,037,777 (GRCm39)	G125S	probably damaging	Het
Ache	T	A	5: 137,288,906 (GRCm39)	L204Q	probably damaging	Het
Acnat2	A	G	4: 49,383,497 (GRCm39)	S19P	probably benign	Het
Adprh	A	G	16: 38,267,638 (GRCm39)	Y216H	probably benign	Het
Anapc1	T	A	2: 128,514,055 (GRCm39)	K429*	probably null	Het
Anxa13	T	C	15: 58,208,353 (GRCm39)		noncoding transcript	Het
Aprt	A	C	8: 123,303,555 (GRCm39)	L6W	probably damaging	Het
Arhgef15	T	C	11: 68,840,820 (GRCm39)	T569A	probably damaging	Het
Atp11a	T	C	8: 12,914,999 (GRCm39)	L1139P	probably benign	Het
Atp2b4	A	G	1: 133,639,467 (GRCm39)	S1136P	probably damaging	Het
B9d1	T	A	11: 61,399,923 (GRCm39)	Y90*	probably null	Het
Btla	G	A	16: 45,059,457 (GRCm39)	A54T	probably damaging	Het
Cd63	T	C	10: 128,747,358 (GRCm39)	V35A	probably benign	Het
Chek2	T	C	5: 110,996,556 (GRCm39)	F173L	probably damaging	Het
Cntnap3	T	A	13: 64,929,702 (GRCm39)	N499I	possibly damaging	Het
Cog4	T	C	8: 111,608,418 (GRCm39)	L738P	probably damaging	Het
Cops6	T	C	5: 138,162,162 (GRCm39)		probably null	Het
Cpeb1	T	A	7: 81,011,417 (GRCm39)	I175F	probably benign	Het
Cyp3a16	C	T	5: 145,392,705 (GRCm39)	D174N	probably benign	Het
Dnah6	T	G	6: 73,051,687 (GRCm39)	I2984L	probably benign	Het
Dock10	A	T	1: 80,564,068 (GRCm39)	I540N	probably damaging	Het
Elf5	A	G	2: 103,269,578 (GRCm39)	Y53C	probably damaging	Het
Elmod2	T	C	8: 84,046,086 (GRCm39)	T164A	probably damaging	Het
Elp1	T	C	4: 56,798,812 (GRCm39)		probably null	Het
Epn3	A	G	11: 94,385,758 (GRCm39)		probably null	Het
Fam151a	A	T	4: 106,591,201 (GRCm39)	I15F	possibly damaging	Het
Gm11115	T	A	5: 88,301,909 (GRCm39)		probably null	Het
Golga3	T	A	5: 110,352,761 (GRCm39)	I884N	probably damaging	Het
Gria2	A	T	3: 80,599,776 (GRCm39)	F703I	probably damaging	Het
Gtf2h3	C	T	5: 124,722,360 (GRCm39)	T121I	probably benign	Het
Gtpbp2	A	T	17: 46,475,037 (GRCm39)		probably null	Het
Herc2	T	C	7: 55,807,078 (GRCm39)	S2419P	probably damaging	Het
Inpp5d	T	C	1: 87,603,972 (GRCm39)		probably benign	Het
Kdm2b	C	A	5: 123,099,532 (GRCm39)	V136F	probably damaging	Het
Klra2	T	A	6: 131,198,839 (GRCm39)	D234V	probably benign	Het
Lct	A	T	1: 128,228,215 (GRCm39)	S1093T	probably benign	Het
Marchf9	A	G	10: 126,892,558 (GRCm39)	L310P	probably benign	Het
Morc1	G	T	16: 48,407,487 (GRCm39)	E668*	probably null	Het
Nbas	A	T	12: 13,455,426 (GRCm39)	L1213F	probably damaging	Het
Neto1	A	G	18: 86,516,873 (GRCm39)	T397A	possibly damaging	Het
Oit3	A	G	10: 59,265,462 (GRCm39)	C268R	probably damaging	Het
Or5p55	A	G	7: 107,566,669 (GRCm39)	T22A	probably benign	Het
Pcx	T	C	19: 4,654,523 (GRCm39)	F312L	probably benign	Het
Pitx2	T	C	3: 129,008,432 (GRCm39)		probably null	Het
Pkhd1l1	G	A	15: 44,361,485 (GRCm39)		probably null	Het
Polr1a	C	A	6: 71,906,427 (GRCm39)	D414E	possibly damaging	Het
Pramel27	A	G	4: 143,579,384 (GRCm39)	D323G	probably damaging	Het
Prkch	T	C	12: 73,749,549 (GRCm39)	Y381H	probably damaging	Het
Ptger3	T	A	3: 157,273,139 (GRCm39)	V162E	probably damaging	Het
Ptgr2	T	G	12: 84,360,726 (GRCm39)	M332R	probably damaging	Het
Ptprq	G	T	10: 107,378,514 (GRCm39)	S2009*	probably null	Het
Pwwp3a	A	G	10: 80,068,113 (GRCm39)	T86A	probably benign	Het
Rangrf	C	T	11: 68,864,514 (GRCm39)	G11R	probably damaging	Het
Rbl2	A	T	8: 91,823,467 (GRCm39)	Q465L	probably benign	Het
Rhbdd1	A	T	1: 82,318,380 (GRCm39)	M88L	probably benign	Het
Setd2	G	A	9: 110,361,785 (GRCm39)	M13I	probably benign	Het
Sirpb1a	T	C	3: 15,444,080 (GRCm39)	Y384C	probably damaging	Het
Slc13a1	C	T	6: 24,097,611 (GRCm39)	G439S	probably damaging	Het
Slc41a1	T	A	1: 131,768,887 (GRCm39)	I239N	probably damaging	Het
Slit2	T	A	5: 48,461,509 (GRCm39)	C1502S	probably damaging	Het
Slit3	G	A	11: 35,552,119 (GRCm39)	E888K	probably benign	Het
Srrm3	G	T	5: 135,864,088 (GRCm39)	R62L	probably damaging	Het
Synm	A	G	7: 67,385,331 (GRCm39)	V777A	possibly damaging	Het
Trmt13	T	A	3: 116,385,864 (GRCm39)	N31I	probably damaging	Het
Trpm8	G	A	1: 88,289,720 (GRCm39)	E893K	probably damaging	Het
Uqcc1	A	G	2: 155,693,343 (GRCm39)	F197S	probably damaging	Het
Vmn1r67	T	A	7: 10,181,598 (GRCm39)	N287K	probably benign	Het
Wwc1	C	T	11: 35,744,264 (GRCm39)	E853K	probably benign	Het
Xdh	C	A	17: 74,207,546 (GRCm39)	C937F	probably damaging	Het
Zfp846	T	A	9: 20,505,167 (GRCm39)	N342K	probably benign	Het

Other mutations in Vmn2r44

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01650:Vmn2r44	APN	7	8,383,103 (GRCm39)	critical splice donor site	probably null
IGL01723:Vmn2r44	APN	7	8,380,915 (GRCm39)	missense	probably damaging	0.97
IGL01767:Vmn2r44	APN	7	8,383,237 (GRCm39)	missense	probably benign	0.00
IGL02161:Vmn2r44	APN	7	8,380,814 (GRCm39)	missense	possibly damaging	0.94
IGL02299:Vmn2r44	APN	7	8,380,815 (GRCm39)	missense	probably benign
IGL02418:Vmn2r44	APN	7	8,380,864 (GRCm39)	missense	probably damaging	1.00
IGL02829:Vmn2r44	APN	7	8,380,879 (GRCm39)	missense	possibly damaging	0.94
IGL02851:Vmn2r44	APN	7	8,386,050 (GRCm39)	missense	probably damaging	1.00
IGL03080:Vmn2r44	APN	7	8,386,244 (GRCm39)	utr 5 prime	probably benign
R1471:Vmn2r44	UTSW	7	8,380,882 (GRCm39)	missense	probably damaging	0.99
R1789:Vmn2r44	UTSW	7	8,383,122 (GRCm39)	missense	possibly damaging	0.64
R1932:Vmn2r44	UTSW	7	8,370,981 (GRCm39)	missense	probably benign	0.04
R2354:Vmn2r44	UTSW	7	8,373,639 (GRCm39)	missense	probably damaging	0.99
R4009:Vmn2r44	UTSW	7	8,380,987 (GRCm39)	missense	possibly damaging	0.82
R4130:Vmn2r44	UTSW	7	8,370,918 (GRCm39)	missense	probably damaging	1.00
R4790:Vmn2r44	UTSW	7	8,370,949 (GRCm39)	missense	probably damaging	1.00
R4854:Vmn2r44	UTSW	7	8,383,300 (GRCm39)	missense	possibly damaging	0.94
R4887:Vmn2r44	UTSW	7	8,380,985 (GRCm39)	missense	probably benign	0.19
R4888:Vmn2r44	UTSW	7	8,380,985 (GRCm39)	missense	probably benign	0.19
R5484:Vmn2r44	UTSW	7	8,383,123 (GRCm39)	missense	possibly damaging	0.69
R6110:Vmn2r44	UTSW	7	8,381,005 (GRCm39)	missense	probably damaging	1.00
R6357:Vmn2r44	UTSW	7	8,373,657 (GRCm39)	missense	probably benign	0.01
R7083:Vmn2r44	UTSW	7	8,381,369 (GRCm39)	missense	probably benign	0.44
R7087:Vmn2r44	UTSW	7	8,381,366 (GRCm39)	missense	probably benign	0.02
R7115:Vmn2r44	UTSW	7	8,370,527 (GRCm39)	nonsense	probably null
R7125:Vmn2r44	UTSW	7	8,370,941 (GRCm39)	missense	probably damaging	1.00
R7258:Vmn2r44	UTSW	7	8,380,848 (GRCm39)	missense	probably damaging	1.00
R7359:Vmn2r44	UTSW	7	8,370,538 (GRCm39)	missense	probably benign	0.30
R7494:Vmn2r44	UTSW	7	8,386,122 (GRCm39)	nonsense	probably null
R7766:Vmn2r44	UTSW	7	8,371,219 (GRCm39)	missense	probably damaging	1.00
R7777:Vmn2r44	UTSW	7	8,381,314 (GRCm39)	missense	possibly damaging	0.81
R8184:Vmn2r44	UTSW	7	8,371,227 (GRCm39)	nonsense	probably null
R8674:Vmn2r44	UTSW	7	8,380,822 (GRCm39)	missense	probably damaging	1.00
R8752:Vmn2r44	UTSW	7	8,370,805 (GRCm39)	missense	probably damaging	1.00
R8897:Vmn2r44	UTSW	7	8,381,242 (GRCm39)	missense	probably damaging	1.00
R8915:Vmn2r44	UTSW	7	8,370,650 (GRCm39)	missense	probably damaging	1.00
R8928:Vmn2r44	UTSW	7	8,381,101 (GRCm39)	missense	probably damaging	1.00
R9163:Vmn2r44	UTSW	7	8,371,091 (GRCm39)	missense	probably benign	0.00
Z1177:Vmn2r44	UTSW	7	8,370,976 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TGAGATGGAACCATGTCTGTAC -3'
(R):5'- CTGGATTGGCCTTGTCATCC -3'

Sequencing Primer
(F):5'- GATGGAACCATGTCTGTACAAAATC -3'
(R):5'- TCATCCCAGATGATGATCAAGG -3'

Posted On

2018-06-06