Mutagenetix > Incidental Mutation

Incidental Mutation 'R6527:Letm2'

521977

Institutional Source

Beutler Lab

Gene Symbol

Letm2

Ensembl Gene

ENSMUSG00000037363

Gene Name

leucine zipper-EF-hand containing transmembrane protein 2

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.106) question?

Stock #

R6527 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

25578490-25597582 bp(-) (GRCm38)

Type of Mutation

utr 3 prime

DNA Base Change (assembly)

A to G at 25592506 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000147648 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079160] [ENSMUST00000210234] [ENSMUST00000210616] [ENSMUST00000210810] [ENSMUST00000211422]

AlphaFold

Q7TNU7

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000059474

Predicted Effect

silent
Transcript: ENSMUST00000079160

SMART Domains

Protein: ENSMUSP00000078160
Gene: ENSMUSG00000037363

Domain	Start	End	E-Value	Type
low complexity region	106	117	N/A	INTRINSIC
Pfam:LETM1	120	384	1.2e-102	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000209539

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000209734

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000209982

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000210221

Predicted Effect

silent
Transcript: ENSMUST00000210234

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000210321

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000210582

Predicted Effect

probably benign
Transcript: ENSMUST00000210616

Predicted Effect

silent
Transcript: ENSMUST00000210810

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000210914

Predicted Effect

unknown
Transcript: ENSMUST00000211422
AA Change: S185P

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000211001

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000211137

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 97.9%
20x: 93.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	G	3: 138,067,527	T826A	probably damaging	Het
Abca16	T	A	7: 120,477,772	I686N	possibly damaging	Het
Abcb6	T	C	1: 75,177,488		probably null	Het
Adgrl3	A	C	5: 81,787,517	E1299A	probably damaging	Het
Als2cr12	T	C	1: 58,692,413	M1V	probably null	Het
Amd2	A	C	10: 35,710,806	Y252D	probably damaging	Het
Cfap74	A	G	4: 155,422,265		probably null	Het
Dhx29	G	T	13: 112,932,542	K135N	probably damaging	Het
Dlg5	T	A	14: 24,190,448	D245V	possibly damaging	Het
Dscaml1	C	T	9: 45,712,184	Q83*	probably null	Het
Dsp	T	A	13: 38,195,873	L1599Q	probably damaging	Het
Duox2	A	G	2: 122,294,614	V369A	probably benign	Het
Gbe1	T	A	16: 70,433,672		probably null	Het
Gm7298	A	G	6: 121,769,710	K600R	probably benign	Het
Heatr4	C	T	12: 83,979,763	G240E	probably damaging	Het
Jakmip2	A	G	18: 43,556,524	V651A	possibly damaging	Het
Jam3	C	T	9: 27,155,344	R8Q	unknown	Het
Lmod3	T	C	6: 97,247,378	D494G	probably benign	Het
Mast2	T	C	4: 116,314,939	D604G	probably damaging	Het
Mif4gd	C	T	11: 115,609,275		probably null	Het
Msr1	T	C	8: 39,624,233	E112G	possibly damaging	Het
Mtus2	A	G	5: 148,277,598		probably null	Het
Muc4	A	G	16: 32,753,433	H1103R	probably benign	Het
Nudt14	T	A	12: 112,934,887	I198F	possibly damaging	Het
Olfr1341	T	A	4: 118,709,848	F147Y	possibly damaging	Het
Olfr353	T	C	2: 36,890,582	T89A	probably benign	Het
Olfr736	T	A	14: 50,393,428	L224*	probably null	Het
Osbpl8	T	C	10: 111,293,205	I884T	probably benign	Het
Podxl2	T	C	6: 88,842,930	N550S	probably damaging	Het
Ppp1r9a	A	G	6: 5,045,949	Y151C	probably damaging	Het
Prss42	T	C	9: 110,800,856	V226A	possibly damaging	Het
Psmd12	A	G	11: 107,488,968	I116V	probably damaging	Het
Psme4	A	C	11: 30,832,175	I872L	probably benign	Het
Rab11fip1	A	T	8: 27,174,392	V65E	probably damaging	Het
Ros1	T	C	10: 52,143,377	N700S	possibly damaging	Het
Slc15a4	G	A	5: 127,596,709	T547M	probably damaging	Het
Slfn3	T	A	11: 83,213,106	C268S	probably benign	Het
Smc5	T	C	19: 23,228,190	Q794R	probably benign	Het
Sqor	A	G	2: 122,809,286	Y434C	probably damaging	Het
Steap4	T	A	5: 7,978,502	L360H	probably damaging	Het
Sycp1	A	G	3: 102,898,887	V496A	probably benign	Het
Tmem161b	T	G	13: 84,272,264	M128R	probably benign	Het
Tmem59l	T	C	8: 70,486,125	E102G	probably damaging	Het
Tnks	A	T	8: 34,873,093	V457D	probably benign	Het
Tomt	A	G	7: 101,900,392	Y230H	probably damaging	Het
Trim37	A	G	11: 87,190,084	N561D	probably damaging	Het
V1ra8	T	A	6: 90,203,313	I166K	probably damaging	Het
Vmn1r56	A	G	7: 5,196,576	V14A	probably benign	Het
Vsig8	T	C	1: 172,560,358	V5A	possibly damaging	Het
Vwa8	A	T	14: 78,947,213	S384C	possibly damaging	Het
Wwc1	C	T	11: 35,853,437	E853K	probably benign	Het
Zfp984	T	C	4: 147,755,924	N157D	probably benign	Het
Zzef1	G	A	11: 72,874,990	D1448N	probably benign	Het

Other mutations in Letm2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02738:Letm2	APN	8	25586773	missense	probably damaging	1.00
selbstlob	UTSW	8	25593961	missense	probably benign	0.03
IGL03098:Letm2	UTSW	8	25581729	missense	possibly damaging	0.73
R0062:Letm2	UTSW	8	25587448	splice site	probably benign
R0062:Letm2	UTSW	8	25587448	splice site	probably benign
R0207:Letm2	UTSW	8	25578770	missense	probably damaging	0.96
R0485:Letm2	UTSW	8	25592558	missense	probably damaging	1.00
R1869:Letm2	UTSW	8	25581713	missense	probably damaging	0.98
R1870:Letm2	UTSW	8	25596444	splice site	probably benign
R1871:Letm2	UTSW	8	25596444	splice site	probably benign
R3881:Letm2	UTSW	8	25593868	nonsense	probably null
R4115:Letm2	UTSW	8	25580327	nonsense	probably null
R4459:Letm2	UTSW	8	25586699	missense	probably damaging	1.00
R4461:Letm2	UTSW	8	25586699	missense	probably damaging	1.00
R4961:Letm2	UTSW	8	25594092	missense	possibly damaging	0.86
R5063:Letm2	UTSW	8	25581779	missense	probably benign	0.26
R5069:Letm2	UTSW	8	25593964	nonsense	probably null
R5732:Letm2	UTSW	8	25587325	missense	possibly damaging	0.51
R6706:Letm2	UTSW	8	25593961	missense	probably benign	0.03
R7624:Letm2	UTSW	8	25592537	nonsense	probably null
R7968:Letm2	UTSW	8	25593750	missense	probably damaging	1.00
R8272:Letm2	UTSW	8	25586656	missense	probably damaging	1.00
R8356:Letm2	UTSW	8	25581713	missense	probably damaging	0.98
R8456:Letm2	UTSW	8	25581713	missense	probably damaging	0.98
R8481:Letm2	UTSW	8	25580359	missense	possibly damaging	0.86
R9023:Letm2	UTSW	8	25587220	missense
R9234:Letm2	UTSW	8	25594086	missense	probably benign	0.03
R9366:Letm2	UTSW	8	25594149	missense	probably damaging	1.00
R9636:Letm2	UTSW	8	25593703	missense	probably benign	0.33
R9690:Letm2	UTSW	8	25587419	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GGGAAGAACTTCAGTTGTTGGGT -3'
(R):5'- AAGGCAGGCTTTGTTTTGGC -3'

Sequencing Primer
(F):5'- CAGTGAGACTATTGCATGTGACCC -3'
(R):5'- CAGTGGGTCTTGGATGGTTC -3'

Posted On

2018-06-06