Mutagenetix > Incidental Mutation

Incidental Mutation 'R6527:Tmem161b'

522019

Institutional Source

Beutler Lab

Gene Symbol

Tmem161b

Ensembl Gene

ENSMUSG00000035762

Gene Name

transmembrane protein 161B

Synonyms

2810446P07Rik

MMRRC Submission

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6527 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

84222296-84296141 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 84272264 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Arginine at position 128 (M128R)

Ref Sequence

ENSEMBL: ENSMUSP00000055208 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000057495] [ENSMUST00000223827] [ENSMUST00000223862] [ENSMUST00000225069]

AlphaFold

Q8C2L6

Predicted Effect

probably benign
Transcript: ENSMUST00000057495
AA Change: M128R

PolyPhen 2 Score 0.064 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000055208
Gene: ENSMUSG00000035762
AA Change: M128R

Domain	Start	End	E-Value	Type
Pfam:Tmemb_161AB	2	485	1.3e-213	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000223827
AA Change: M128R

PolyPhen 2 Score 0.021 (Sensitivity: 0.95; Specificity: 0.80)

Predicted Effect

probably benign
Transcript: ENSMUST00000223862
AA Change: M128R

PolyPhen 2 Score 0.038 (Sensitivity: 0.94; Specificity: 0.82)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000224501

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000224744

Predicted Effect

probably benign
Transcript: ENSMUST00000225069

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000225126

Predicted Effect

unknown
Transcript: ENSMUST00000225708
AA Change: M113R

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 97.9%
20x: 93.7%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Homozygous mice exhibit lethality before genotyping age. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	G	3: 138,067,527	T826A	probably damaging	Het
Abca16	T	A	7: 120,477,772	I686N	possibly damaging	Het
Abcb6	T	C	1: 75,177,488		probably null	Het
Adgrl3	A	C	5: 81,787,517	E1299A	probably damaging	Het
Als2cr12	T	C	1: 58,692,413	M1V	probably null	Het
Amd2	A	C	10: 35,710,806	Y252D	probably damaging	Het
Cfap74	A	G	4: 155,422,265		probably null	Het
Dhx29	G	T	13: 112,932,542	K135N	probably damaging	Het
Dlg5	T	A	14: 24,190,448	D245V	possibly damaging	Het
Dscaml1	C	T	9: 45,712,184	Q83*	probably null	Het
Dsp	T	A	13: 38,195,873	L1599Q	probably damaging	Het
Duox2	A	G	2: 122,294,614	V369A	probably benign	Het
Gbe1	T	A	16: 70,433,672		probably null	Het
Gm7298	A	G	6: 121,769,710	K600R	probably benign	Het
Heatr4	C	T	12: 83,979,763	G240E	probably damaging	Het
Jakmip2	A	G	18: 43,556,524	V651A	possibly damaging	Het
Jam3	C	T	9: 27,155,344	R8Q	unknown	Het
Letm2	A	G	8: 25,592,506		probably benign	Het
Lmod3	T	C	6: 97,247,378	D494G	probably benign	Het
Mast2	T	C	4: 116,314,939	D604G	probably damaging	Het
Mif4gd	C	T	11: 115,609,275		probably null	Het
Msr1	T	C	8: 39,624,233	E112G	possibly damaging	Het
Mtus2	A	G	5: 148,277,598		probably null	Het
Muc4	A	G	16: 32,753,433	H1103R	probably benign	Het
Nudt14	T	A	12: 112,934,887	I198F	possibly damaging	Het
Olfr1341	T	A	4: 118,709,848	F147Y	possibly damaging	Het
Olfr353	T	C	2: 36,890,582	T89A	probably benign	Het
Olfr736	T	A	14: 50,393,428	L224*	probably null	Het
Osbpl8	T	C	10: 111,293,205	I884T	probably benign	Het
Podxl2	T	C	6: 88,842,930	N550S	probably damaging	Het
Ppp1r9a	A	G	6: 5,045,949	Y151C	probably damaging	Het
Prss42	T	C	9: 110,800,856	V226A	possibly damaging	Het
Psmd12	A	G	11: 107,488,968	I116V	probably damaging	Het
Psme4	A	C	11: 30,832,175	I872L	probably benign	Het
Rab11fip1	A	T	8: 27,174,392	V65E	probably damaging	Het
Ros1	T	C	10: 52,143,377	N700S	possibly damaging	Het
Slc15a4	G	A	5: 127,596,709	T547M	probably damaging	Het
Slfn3	T	A	11: 83,213,106	C268S	probably benign	Het
Smc5	T	C	19: 23,228,190	Q794R	probably benign	Het
Sqor	A	G	2: 122,809,286	Y434C	probably damaging	Het
Steap4	T	A	5: 7,978,502	L360H	probably damaging	Het
Sycp1	A	G	3: 102,898,887	V496A	probably benign	Het
Tmem59l	T	C	8: 70,486,125	E102G	probably damaging	Het
Tnks	A	T	8: 34,873,093	V457D	probably benign	Het
Tomt	A	G	7: 101,900,392	Y230H	probably damaging	Het
Trim37	A	G	11: 87,190,084	N561D	probably damaging	Het
V1ra8	T	A	6: 90,203,313	I166K	probably damaging	Het
Vmn1r56	A	G	7: 5,196,576	V14A	probably benign	Het
Vsig8	T	C	1: 172,560,358	V5A	possibly damaging	Het
Vwa8	A	T	14: 78,947,213	S384C	possibly damaging	Het
Wwc1	C	T	11: 35,853,437	E853K	probably benign	Het
Zfp984	T	C	4: 147,755,924	N157D	probably benign	Het
Zzef1	G	A	11: 72,874,990	D1448N	probably benign	Het

Other mutations in Tmem161b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00666:Tmem161b	APN	13	84294596	splice site	probably benign
IGL01086:Tmem161b	APN	13	84222422	utr 5 prime	probably benign
IGL01150:Tmem161b	APN	13	84292407	nonsense	probably null
IGL01566:Tmem161b	APN	13	84294762	missense	probably benign	0.35
IGL02183:Tmem161b	APN	13	84272254	missense	probably damaging	1.00
IGL02481:Tmem161b	APN	13	84283993	missense	probably damaging	0.99
IGL02519:Tmem161b	APN	13	84294744	missense	probably damaging	1.00
IGL03207:Tmem161b	APN	13	84294595	splice site	probably benign
R6836_Tmem161b_587	UTSW	13	84222418	start gained	probably benign
R0015:Tmem161b	UTSW	13	84222414	splice site	probably null
R0376:Tmem161b	UTSW	13	84292383	missense	probably benign	0.43
R0613:Tmem161b	UTSW	13	84251320	missense	probably damaging	1.00
R1925:Tmem161b	UTSW	13	84260229	missense	probably benign	0.07
R1935:Tmem161b	UTSW	13	84293466	missense	probably damaging	1.00
R1936:Tmem161b	UTSW	13	84293466	missense	probably damaging	1.00
R2325:Tmem161b	UTSW	13	84294768	missense	possibly damaging	0.94
R2988:Tmem161b	UTSW	13	84292455	nonsense	probably null
R4327:Tmem161b	UTSW	13	84251240	missense	probably damaging	1.00
R4525:Tmem161b	UTSW	13	84257802	missense	probably benign	0.00
R4558:Tmem161b	UTSW	13	84251244	missense	possibly damaging	0.50
R5133:Tmem161b	UTSW	13	84294768	missense	possibly damaging	0.94
R5134:Tmem161b	UTSW	13	84294768	missense	possibly damaging	0.94
R5727:Tmem161b	UTSW	13	84286790	missense	possibly damaging	0.63
R5875:Tmem161b	UTSW	13	84294858	missense	probably damaging	1.00
R6217:Tmem161b	UTSW	13	84251244	missense	possibly damaging	0.50
R6550:Tmem161b	UTSW	13	84222418	start gained	probably benign
R6551:Tmem161b	UTSW	13	84222418	start gained	probably benign
R6553:Tmem161b	UTSW	13	84222418	start gained	probably benign
R6554:Tmem161b	UTSW	13	84222418	start gained	probably benign
R6640:Tmem161b	UTSW	13	84222418	start gained	probably benign
R6641:Tmem161b	UTSW	13	84222418	start gained	probably benign
R6685:Tmem161b	UTSW	13	84222418	start gained	probably benign
R6836:Tmem161b	UTSW	13	84222418	start gained	probably benign
R6837:Tmem161b	UTSW	13	84222418	start gained	probably benign
R6838:Tmem161b	UTSW	13	84222418	start gained	probably benign
R7077:Tmem161b	UTSW	13	84222418	start gained	probably benign
R7078:Tmem161b	UTSW	13	84222418	start gained	probably benign
R7386:Tmem161b	UTSW	13	84222418	start gained	probably benign
R7388:Tmem161b	UTSW	13	84222418	start gained	probably benign
R7429:Tmem161b	UTSW	13	84282747	critical splice acceptor site	probably null
R7430:Tmem161b	UTSW	13	84282747	critical splice acceptor site	probably null
R7547:Tmem161b	UTSW	13	84222418	start gained	probably benign
R7548:Tmem161b	UTSW	13	84222418	start gained	probably benign
R7634:Tmem161b	UTSW	13	84222418	start gained	probably benign
R7636:Tmem161b	UTSW	13	84222418	start gained	probably benign
R8094:Tmem161b	UTSW	13	84222418	start gained	probably benign
R8095:Tmem161b	UTSW	13	84222418	start gained	probably benign
R8255:Tmem161b	UTSW	13	84222418	start gained	probably benign
R8257:Tmem161b	UTSW	13	84222418	start gained	probably benign
R8669:Tmem161b	UTSW	13	84272169	critical splice acceptor site	probably null
R9049:Tmem161b	UTSW	13	84294635	missense	probably benign
R9092:Tmem161b	UTSW	13	84292384	missense	possibly damaging	0.69
R9316:Tmem161b	UTSW	13	84282736	missense	possibly damaging	0.84
R9326:Tmem161b	UTSW	13	84292483	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TGGAGTCTAACATACTTACTGTTCC -3'
(R):5'- AACTGTCTGCTAGCCTTTCTGATATG -3'

Sequencing Primer
(F):5'- CCATAGATTTATCCTCATAAGCTCAC -3'
(R):5'- AGCCTTTCTGATATGCTACATGG -3'

Posted On

2018-06-06