Mutagenetix > Incidental Mutation

Incidental Mutation 'R6530:Rasl10a'

522283

Institutional Source

Beutler Lab

Gene Symbol

Rasl10a

Ensembl Gene

ENSMUSG00000034209

Gene Name

RAS-like, family 10, member A

Synonyms

2210403B10Rik

MMRRC Submission

044656-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.116) question?

Stock #

R6530 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

5008128-5010385 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 5008367 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 21 (I21N)

Ref Sequence

ENSEMBL: ENSMUSP00000048453 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000037146] [ENSMUST00000037218] [ENSMUST00000056649] [ENSMUST00000109895]

AlphaFold

Q8K5A4

Predicted Effect

probably benign
Transcript: ENSMUST00000037146

SMART Domains

Protein: ENSMUSP00000043709
Gene: ENSMUSG00000034201

Domain	Start	End	E-Value	Type
CH	29	143	2.69e-16	SMART
GAS2	206	278	7.69e-52	SMART
low complexity region	292	318	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000037218
AA Change: I21N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000048453
Gene: ENSMUSG00000034209
AA Change: I21N

Domain	Start	End	E-Value	Type
Pfam:Ras	6	180	1.5e-20	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000056649

SMART Domains

Protein: ENSMUSP00000050275
Gene: ENSMUSG00000034201

Domain	Start	End	E-Value	Type
CH	29	143	2.69e-16	SMART
GAS2	206	278	7.69e-52	SMART
low complexity region	292	318	N/A	INTRINSIC
low complexity region	335	365	N/A	INTRINSIC
low complexity region	392	406	N/A	INTRINSIC
low complexity region	411	423	N/A	INTRINSIC
low complexity region	460	473	N/A	INTRINSIC
low complexity region	478	496	N/A	INTRINSIC
low complexity region	509	539	N/A	INTRINSIC
low complexity region	546	568	N/A	INTRINSIC
low complexity region	641	657	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000109895

SMART Domains

Protein: ENSMUSP00000105521
Gene: ENSMUSG00000034201

Domain	Start	End	E-Value	Type
CH	29	143	2.69e-16	SMART
GAS2	206	278	7.69e-52	SMART
low complexity region	292	318	N/A	INTRINSIC
low complexity region	335	365	N/A	INTRINSIC
low complexity region	392	406	N/A	INTRINSIC
low complexity region	411	423	N/A	INTRINSIC
low complexity region	460	473	N/A	INTRINSIC
low complexity region	478	496	N/A	INTRINSIC
low complexity region	509	539	N/A	INTRINSIC
low complexity region	546	568	N/A	INTRINSIC
low complexity region	641	657	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000122886

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126974

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000156196

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.5%
20x: 92.1%

Validation Efficiency

100% (36/36)

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acox3	T	C	5: 35,746,039 (GRCm39)	L74P	possibly damaging	Het
Adamtsl4	T	C	3: 95,588,364 (GRCm39)	T575A	probably benign	Het
Adck5	T	G	15: 76,478,047 (GRCm39)	D224E	probably benign	Het
Asic4	A	G	1: 75,448,979 (GRCm39)	N376S	probably damaging	Het
Atl3	T	A	19: 7,499,499 (GRCm39)	D254E	probably benign	Het
Cacnb2	G	T	2: 14,979,978 (GRCm39)	A274S	probably damaging	Het
Car2	T	C	3: 14,961,791 (GRCm39)	V159A	probably benign	Het
Ccdc138	G	C	10: 58,380,790 (GRCm39)	G474R	probably damaging	Het
Coq7	T	C	7: 118,124,558 (GRCm39)	T203A	probably benign	Het
Dnah12	T	C	14: 26,456,865 (GRCm39)	I877T	probably damaging	Het
Dnah7a	C	T	1: 53,542,856 (GRCm39)	R2438H	probably benign	Het
Efhc1	T	A	1: 21,031,366 (GRCm39)		probably null	Het
Fbn1	G	A	2: 125,231,190 (GRCm39)	R459C	probably damaging	Het
Fer1l4	A	G	2: 155,889,785 (GRCm39)		probably null	Het
Gm5114	G	A	7: 39,057,514 (GRCm39)	P702S	probably damaging	Het
Inpp5f	T	A	7: 128,265,802 (GRCm39)	Y182*	probably null	Het
Irf6	C	T	1: 192,839,657 (GRCm39)	T44M	probably damaging	Het
Loxhd1	A	G	18: 77,499,847 (GRCm39)	N87S	probably benign	Het
Mtrf1	A	T	14: 79,640,331 (GRCm39)	Q162L	possibly damaging	Het
Myoz3	G	T	18: 60,712,592 (GRCm39)		probably null	Het
Ncbp2	CGTCTGGATG	CG	16: 31,775,161 (GRCm39)		probably null	Het
Nkd2	C	T	13: 73,970,809 (GRCm39)	G258R	probably null	Het
Or8k27	A	C	2: 86,275,826 (GRCm39)	S167A	probably benign	Het
Parg	T	C	14: 31,931,156 (GRCm39)	S176P	probably damaging	Het
Prdm2	A	C	4: 142,860,617 (GRCm39)	V891G	probably benign	Het
Reg4	T	C	3: 98,132,148 (GRCm39)	V20A	probably benign	Het
Shprh	T	A	10: 11,070,011 (GRCm39)	S1462R	probably benign	Het
Spmip1	G	A	6: 29,471,950 (GRCm39)		probably null	Het
Sult1e1	T	C	5: 87,724,147 (GRCm39)	E270G	probably benign	Het
Trpm7	A	C	2: 126,654,631 (GRCm39)	F1436V	probably damaging	Het
Ttc33	A	G	15: 5,241,603 (GRCm39)		probably null	Het
Ucp2	A	T	7: 100,147,430 (GRCm39)	E161D	probably benign	Het
Vmn2r79	T	C	7: 86,651,252 (GRCm39)	F217S	possibly damaging	Het
Wdr93	G	A	7: 79,405,741 (GRCm39)	A207T	probably damaging	Het
Zbed6	A	G	1: 133,586,939 (GRCm39)	S133P	probably damaging	Het
Zfp949	T	C	9: 88,449,340 (GRCm39)		probably null	Het

Other mutations in Rasl10a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02233:Rasl10a	APN	11	5,008,333 (GRCm39)	missense	probably damaging	0.99
IGL03149:Rasl10a	APN	11	5,008,429 (GRCm39)	missense	possibly damaging	0.80
R1630:Rasl10a	UTSW	11	5,009,542 (GRCm39)	missense	probably damaging	0.99
R1678:Rasl10a	UTSW	11	5,009,815 (GRCm39)	missense	possibly damaging	0.61
R1925:Rasl10a	UTSW	11	5,009,473 (GRCm39)	missense	possibly damaging	0.84
R2086:Rasl10a	UTSW	11	5,009,431 (GRCm39)	critical splice acceptor site	probably null
R3792:Rasl10a	UTSW	11	5,009,461 (GRCm39)	missense	probably damaging	0.99
R4482:Rasl10a	UTSW	11	5,008,429 (GRCm39)	missense	probably damaging	1.00
R4719:Rasl10a	UTSW	11	5,008,517 (GRCm39)	missense	probably benign	0.03
R5743:Rasl10a	UTSW	11	5,009,519 (GRCm39)	missense	probably benign	0.02
R6168:Rasl10a	UTSW	11	5,008,442 (GRCm39)	missense	possibly damaging	0.76
R6684:Rasl10a	UTSW	11	5,008,396 (GRCm39)	missense	possibly damaging	0.49
R8049:Rasl10a	UTSW	11	5,009,823 (GRCm39)	missense	probably damaging	0.98
R9144:Rasl10a	UTSW	11	5,008,473 (GRCm39)	missense	probably benign	0.31

Predicted Primers

PCR Primer
(F):5'- TTTTGAAACGGTTGAGGGAAGC -3'
(R):5'- GGATCAGTACACATGGGCAC -3'

Sequencing Primer
(F):5'- GCTTTAACAAGGCGACCCG -3'
(R):5'- TGTACACACACCCCTGGC -3'

Posted On

2018-06-06