Incidental Mutation 'R6531:Cd200r4'
ID522384
Institutional Source Beutler Lab
Gene Symbol Cd200r4
Ensembl Gene ENSMUSG00000062082
Gene NameCD200 receptor 4
SynonymsF630107N04Rik, MCD200RLa
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.054) question?
Stock #R6531 (G1)
Quality Score225.009
Status Validated
Chromosome16
Chromosomal Location44811733-44839150 bp(+) (GRCm38)
Type of Mutationnonsense
DNA Base Change (assembly) C to T at 44833505 bp
ZygosityHeterozygous
Amino Acid Change Glutamine to Stop codon at position 222 (Q222*)
Ref Sequence ENSEMBL: ENSMUSP00000135299 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000114626] [ENSMUST00000176321] [ENSMUST00000176819]
Predicted Effect probably null
Transcript: ENSMUST00000114626
AA Change: Q222*
SMART Domains Protein: ENSMUSP00000110273
Gene: ENSMUSG00000062082
AA Change: Q222*

DomainStartEndE-ValueType
signal peptide 1 25 N/A INTRINSIC
IG 44 147 1.23e-3 SMART
Blast:IG_like 149 270 2e-68 BLAST
Predicted Effect probably null
Transcript: ENSMUST00000176321
AA Change: Q176*
SMART Domains Protein: ENSMUSP00000135162
Gene: ENSMUSG00000062082
AA Change: Q176*

DomainStartEndE-ValueType
IG 1 101 1.97e0 SMART
Blast:IG_like 103 224 6e-69 BLAST
Predicted Effect probably null
Transcript: ENSMUST00000176819
AA Change: Q222*
SMART Domains Protein: ENSMUSP00000135299
Gene: ENSMUSG00000062082
AA Change: Q222*

DomainStartEndE-ValueType
signal peptide 1 25 N/A INTRINSIC
IG 44 147 1.23e-3 SMART
Blast:IG_like 149 270 2e-68 BLAST
Meta Mutation Damage Score 0.9755 question?
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.5%
  • 10x: 97.2%
  • 20x: 90.6%
Validation Efficiency 100% (46/46)
Allele List at MGI
Other mutations in this stock
Total: 44 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930590J08Rik A G 6: 91,949,999 E880G possibly damaging Het
Acsbg2 T A 17: 56,846,617 I529F probably damaging Het
Ahcyl2 T G 6: 29,886,162 M359R probably benign Het
Aldh5a1 G T 13: 24,918,564 D305E probably benign Het
Catsper2 C G 2: 121,399,780 V358L possibly damaging Het
Col4a2 T A 8: 11,408,135 D270E probably benign Het
Cux1 T C 5: 136,275,119 D1401G probably benign Het
Cyp3a59 T A 5: 146,098,217 M235K probably benign Het
Dock3 T C 9: 106,967,216 D895G probably benign Het
Dusp27 A T 1: 166,110,046 probably null Het
Dync1h1 T C 12: 110,617,920 F586L probably damaging Het
Elmo1 G T 13: 20,572,446 R568L possibly damaging Het
Epb41 T C 4: 131,957,636 T711A probably benign Het
Grm7 T A 6: 111,358,425 M599K probably benign Het
Hivep3 A T 4: 120,122,876 K1704* probably null Het
Ighv1-62-3 C A 12: 115,461,006 C115F probably damaging Het
Krt78 A G 15: 101,952,273 Y200H probably benign Het
Lamb2 A T 9: 108,483,726 H549L possibly damaging Het
Mroh3 A G 1: 136,184,353 I759T probably benign Het
Ncbp2 CGTCTGGATG CG 16: 31,956,343 probably null Het
Nol6 G T 4: 41,118,154 P828T probably benign Het
Olfr1030 A G 2: 85,984,307 I156V probably benign Het
Olfr1132 A G 2: 87,635,529 Y73H probably damaging Het
Olfr1264 A G 2: 90,021,457 V203A probably benign Het
Olfr344 G A 2: 36,569,341 V248I probably damaging Het
Ovgp1 A C 3: 105,987,071 probably benign Het
Pitpnm3 T A 11: 72,071,487 Q230L possibly damaging Het
Pkn1 C T 8: 83,670,293 V910I probably benign Het
Plcb1 T A 2: 135,325,802 probably null Het
Ppp1r12c A G 7: 4,482,789 probably null Het
Rassf5 T A 1: 131,244,814 Q106L possibly damaging Het
Rfc1 T C 5: 65,312,979 K62E possibly damaging Het
Sf3b1 C T 1: 55,019,395 E12K probably damaging Het
Slc4a1ap A T 5: 31,548,638 D691V probably benign Het
Speg T A 1: 75,422,757 F2283I probably benign Het
Synj2 A G 17: 6,033,839 K267E probably damaging Het
Tg A T 15: 66,839,362 Y991F probably damaging Het
Tlk1 A T 2: 70,742,083 D380E probably benign Het
Trim43b A T 9: 89,085,365 L405H probably damaging Het
Ttf2 A G 3: 100,956,260 I586T probably damaging Het
Ugt2b36 T A 5: 87,081,586 R213S probably damaging Het
Vmn1r198 T A 13: 22,354,407 M21K probably benign Het
Wdr35 A T 12: 8,978,685 Y101F probably benign Het
Zfp367 T C 13: 64,144,250 Y189C probably damaging Het
Other mutations in Cd200r4
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02996:Cd200r4 APN 16 44833033 missense probably benign 0.08
IGL03328:Cd200r4 APN 16 44833519 missense possibly damaging 0.91
H8562:Cd200r4 UTSW 16 44833373 missense possibly damaging 0.62
H8786:Cd200r4 UTSW 16 44833373 missense possibly damaging 0.62
R1163:Cd200r4 UTSW 16 44838020 missense probably benign 0.03
R1168:Cd200r4 UTSW 16 44832944 missense probably damaging 1.00
R1502:Cd200r4 UTSW 16 44833440 missense probably damaging 1.00
R1536:Cd200r4 UTSW 16 44833049 missense possibly damaging 0.95
R2237:Cd200r4 UTSW 16 44820897 start codon destroyed probably null 1.00
R2239:Cd200r4 UTSW 16 44820897 start codon destroyed probably null 1.00
R2899:Cd200r4 UTSW 16 44833365 missense probably damaging 1.00
R3824:Cd200r4 UTSW 16 44820950 missense probably benign 0.00
R3825:Cd200r4 UTSW 16 44820950 missense probably benign 0.00
R4295:Cd200r4 UTSW 16 44832876 missense probably damaging 0.99
R5254:Cd200r4 UTSW 16 44832090 missense possibly damaging 0.86
R5683:Cd200r4 UTSW 16 44832948 missense probably benign 0.01
R5979:Cd200r4 UTSW 16 44832932 missense probably benign 0.14
R7205:Cd200r4 UTSW 16 44833166 missense probably damaging 1.00
R7583:Cd200r4 UTSW 16 44833421 missense probably damaging 1.00
X0063:Cd200r4 UTSW 16 44820906 missense possibly damaging 0.95
Predicted Primers PCR Primer
(F):5'- CAGAACTGCAGTCTGTGAGG -3'
(R):5'- AGTCTGCTGAGCTAGAAAACAG -3'

Sequencing Primer
(F):5'- ACTGCAGTCTGTGAGGCAATG -3'
(R):5'- GAAAACAGCTTCATGTTTTCTGC -3'
Posted On2018-06-06