Mutagenetix > Incidental Mutation

Incidental Mutation 'R6493:Or51a25'

522854

Institutional Source

Beutler Lab

Gene Symbol

Or51a25

Ensembl Gene

ENSMUSG00000066272

Gene Name

olfactory receptor family 51 subfamily A member 25

Synonyms

MOR11-1, Olfr559, GA_x6K02T2PBJ9-5441154-5440198

MMRRC Submission

044625-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R6493 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

102372739-102373695 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 102373287 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Serine at position 137 (R137S)

Ref Sequence

ENSEMBL: ENSMUSP00000151100 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000084816] [ENSMUST00000215657]

AlphaFold

Q8VH14

Predicted Effect

possibly damaging
Transcript: ENSMUST00000084816
AA Change: R137S

PolyPhen 2 Score 0.846 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000095820
Gene: ENSMUSG00000066272
AA Change: R137S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	37	317	1.1e-117	PFAM
Pfam:7TM_GPCR_Srsx	41	215	1e-10	PFAM
Pfam:7tm_1	47	299	3e-18	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000215657
AA Change: R137S

PolyPhen 2 Score 0.846 (Sensitivity: 0.83; Specificity: 0.93)

Coding Region Coverage

1x: 99.8%
3x: 99.4%
10x: 97.3%
20x: 91.3%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca5	T	A	11: 110,184,704 (GRCm39)	H934L	probably benign	Het
Abcb1b	G	A	5: 8,874,698 (GRCm39)	D453N	probably damaging	Het
Ampd3	A	G	7: 110,395,018 (GRCm39)		probably null	Het
Axdnd1	A	T	1: 156,208,383 (GRCm39)	I485N	probably damaging	Het
Bscl2	A	G	19: 8,817,138 (GRCm39)	D22G	probably damaging	Het
Ccdc96	A	G	5: 36,643,596 (GRCm39)	E534G	probably damaging	Het
Cdc42bpg	G	T	19: 6,368,485 (GRCm39)	G1061V	probably damaging	Het
Cers3	T	C	7: 66,393,468 (GRCm39)	Y26H	probably benign	Het
Chd1l	G	A	3: 97,494,483 (GRCm39)	A399V	probably damaging	Het
Clca4a	T	A	3: 144,663,059 (GRCm39)	T597S	probably benign	Het
Daxx	T	A	17: 34,131,345 (GRCm39)		probably null	Het
Egf	T	C	3: 129,512,737 (GRCm39)		probably benign	Het
Eps15	A	G	4: 109,226,145 (GRCm39)	D629G	probably damaging	Het
Fancm	G	A	12: 65,144,262 (GRCm39)	A575T	probably benign	Het
Fat4	G	A	3: 38,945,036 (GRCm39)	D1310N	probably damaging	Het
Gm11595	G	A	11: 99,663,381 (GRCm39)	R100C	unknown	Het
Grxcr2	G	A	18: 42,131,766 (GRCm39)	P101L	possibly damaging	Het
Hsd17b12	A	T	2: 93,874,228 (GRCm39)	I225N	probably damaging	Het
Kcnj8	T	C	6: 142,511,773 (GRCm39)	Y278C	probably damaging	Het
Lama3	G	A	18: 12,615,205 (GRCm39)		probably null	Het
Lax1	C	T	1: 133,607,530 (GRCm39)	D404N	probably benign	Het
Lipg	T	C	18: 75,081,095 (GRCm39)	K329E	probably damaging	Het
Lysmd2	T	G	9: 75,542,984 (GRCm39)	L197R	probably damaging	Het
Maneal	A	T	4: 124,750,964 (GRCm39)	I264N	probably damaging	Het
Myh4	A	G	11: 67,149,455 (GRCm39)	N1729S	probably benign	Het
Myl2	A	G	5: 122,244,791 (GRCm39)	N154D	possibly damaging	Het
Nlrp9c	T	A	7: 26,081,812 (GRCm39)	D638V	probably damaging	Het
Notch1	T	C	2: 26,362,110 (GRCm39)	H74R	unknown	Het
Olfml3	A	T	3: 103,643,523 (GRCm39)	V286D	possibly damaging	Het
Or14c46	T	G	7: 85,918,090 (GRCm39)	K302N	probably benign	Het
Or4b12	G	A	2: 90,096,052 (GRCm39)	H241Y	probably damaging	Het
Or51f23c-ps1	A	G	7: 102,431,603 (GRCm39)	K307E	probably benign	Het
Or8k41	T	C	2: 86,313,565 (GRCm39)	I174V	possibly damaging	Het
Parn	A	C	16: 13,474,789 (GRCm39)	F127V	probably damaging	Het
Pcdhac2	T	C	18: 37,277,758 (GRCm39)	V246A	probably damaging	Het
Plin2	G	A	4: 86,580,224 (GRCm39)	T90I	possibly damaging	Het
Podxl	C	T	6: 31,501,981 (GRCm39)	C326Y	probably damaging	Het
Pramel46	A	G	5: 95,418,684 (GRCm39)	L104P	probably damaging	Het
Prl8a8	T	A	13: 27,691,335 (GRCm39)	K223*	probably null	Het
Ptprh	T	G	7: 4,583,989 (GRCm39)	E201A	possibly damaging	Het
Rtn1	A	C	12: 72,355,103 (GRCm39)	V281G	probably damaging	Het
Scaf8	T	C	17: 3,221,394 (GRCm39)	S294P	unknown	Het
Shroom3	C	A	5: 93,089,420 (GRCm39)	N723K	probably benign	Het
Spata22	T	C	11: 73,244,572 (GRCm39)	*312R	probably null	Het
Stx7	T	C	10: 24,060,969 (GRCm39)		probably null	Het
Taar6	T	C	10: 23,861,021 (GRCm39)	D175G	probably benign	Het
Tbccd1	A	C	16: 22,641,216 (GRCm39)	L387R	probably damaging	Het
Tecpr1	T	C	5: 144,146,792 (GRCm39)	M525V	probably benign	Het
Tex10	A	T	4: 48,436,450 (GRCm39)	I756N	probably damaging	Het
Tfap2d	G	C	1: 19,174,702 (GRCm39)	G52R	probably benign	Het
Ttn	T	C	2: 76,572,341 (GRCm39)	E24438G	probably damaging	Het
Tut4	A	G	4: 108,384,002 (GRCm39)	K1053R	probably damaging	Het
Wdpcp	C	T	11: 21,661,631 (GRCm39)	T301I	possibly damaging	Het
Zbtb1	C	T	12: 76,433,247 (GRCm39)	T411I	probably benign	Het
Zzef1	G	A	11: 72,804,129 (GRCm39)	G2624R	probably benign	Het

Other mutations in Or51a25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02832:Or51a25	APN	7	102,372,858 (GRCm39)	missense	probably benign	0.01
R0505:Or51a25	UTSW	7	102,373,236 (GRCm39)	missense	probably damaging	0.97
R1131:Or51a25	UTSW	7	102,372,887 (GRCm39)	missense	probably damaging	0.99
R1449:Or51a25	UTSW	7	102,373,397 (GRCm39)	missense	probably damaging	1.00
R3788:Or51a25	UTSW	7	102,372,694 (GRCm39)	splice site	probably null
R3915:Or51a25	UTSW	7	102,373,409 (GRCm39)	missense	possibly damaging	0.95
R3980:Or51a25	UTSW	7	102,372,959 (GRCm39)	missense	probably damaging	0.98
R4647:Or51a25	UTSW	7	102,373,299 (GRCm39)	missense	probably damaging	1.00
R5242:Or51a25	UTSW	7	102,373,483 (GRCm39)	missense	probably benign	0.41
R5404:Or51a25	UTSW	7	102,372,807 (GRCm39)	missense	possibly damaging	0.95
R5464:Or51a25	UTSW	7	102,373,124 (GRCm39)	missense	possibly damaging	0.85
R5807:Or51a25	UTSW	7	102,373,409 (GRCm39)	missense	possibly damaging	0.95
R5965:Or51a25	UTSW	7	102,373,467 (GRCm39)	missense	probably benign	0.00
R6008:Or51a25	UTSW	7	102,373,574 (GRCm39)	missense	probably damaging	1.00
R6036:Or51a25	UTSW	7	102,373,692 (GRCm39)	missense	probably benign
R6036:Or51a25	UTSW	7	102,373,692 (GRCm39)	missense	probably benign
R6756:Or51a25	UTSW	7	102,373,295 (GRCm39)	missense	probably benign	0.03
R7201:Or51a25	UTSW	7	102,373,692 (GRCm39)	missense	probably benign
R7460:Or51a25	UTSW	7	102,373,028 (GRCm39)	missense	probably benign	0.09
R7695:Or51a25	UTSW	7	102,372,866 (GRCm39)	missense	probably benign	0.37
R8145:Or51a25	UTSW	7	102,372,937 (GRCm39)	missense	probably damaging	0.98
R8371:Or51a25	UTSW	7	102,372,790 (GRCm39)	missense	probably damaging	1.00
R8499:Or51a25	UTSW	7	102,372,932 (GRCm39)	missense	probably damaging	1.00
R9185:Or51a25	UTSW	7	102,373,409 (GRCm39)	missense	possibly damaging	0.95
R9230:Or51a25	UTSW	7	102,372,795 (GRCm39)	missense	possibly damaging	0.95
X0025:Or51a25	UTSW	7	102,373,022 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GACAGTAATGTCAGCACAGGCC -3'
(R):5'- TCTGTCCATGCTAGCCCTAAG -3'

Sequencing Primer
(F):5'- CAGCTTCATGACATCAGCATGG -3'
(R):5'- GTCCATGCTAGCCCTAAGTGATG -3'

Posted On

2018-06-06