Mutagenetix > Incidental Mutation

Incidental Mutation 'R6491:Vmn2r105'

522901

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r105

Ensembl Gene

ENSMUSG00000091670

Gene Name

vomeronasal 2, receptor 105

Synonyms

EG627743

MMRRC Submission

044623-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6491 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

20428492-20455134 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 20447992 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 277 (Y277*)

Ref Sequence

ENSEMBL: ENSMUSP00000129762 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167382]

AlphaFold

E9Q3A5

Predicted Effect

probably null
Transcript: ENSMUST00000167382
AA Change: Y277*

SMART Domains

Protein: ENSMUSP00000129762
Gene: ENSMUSG00000091670
AA Change: Y277*

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	85	469	6.5e-42	PFAM
Pfam:NCD3G	512	565	3.2e-21	PFAM
Pfam:7tm_3	598	833	2.5e-51	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.1%
20x: 94.5%

Validation Efficiency

100% (35/35)

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4833420G17Rik	T	C	13: 119,612,508 (GRCm39)	L431P	probably damaging	Het
Acad10	A	C	5: 121,768,220 (GRCm39)	W779G	probably damaging	Het
Acrbp	A	G	6: 125,028,442 (GRCm39)		probably benign	Het
Ank3	G	A	10: 69,827,459 (GRCm39)	A565T	probably benign	Het
Ap2a1	A	T	7: 44,565,588 (GRCm39)	I93K	probably damaging	Het
Arnt	T	A	3: 95,383,454 (GRCm39)	M240K	probably damaging	Het
Ceacam12	A	T	7: 17,803,185 (GRCm39)	K197M	probably damaging	Het
Cep41	T	C	6: 30,656,483 (GRCm39)	N323S	probably benign	Het
Dst	A	G	1: 34,232,093 (GRCm39)	T2904A	probably benign	Het
Eml1	G	A	12: 108,479,330 (GRCm39)		probably null	Het
Fbln2	A	G	6: 91,236,732 (GRCm39)	N749S	possibly damaging	Het
Irf2bpl	A	G	12: 86,930,238 (GRCm39)	V145A	probably benign	Het
Itga2b	T	C	11: 102,350,695 (GRCm39)		probably null	Het
Itga8	T	C	2: 12,209,587 (GRCm39)	D466G	probably damaging	Het
Kdm4c	G	T	4: 74,291,873 (GRCm39)	C830F	probably damaging	Het
Mrps27	T	C	13: 99,499,538 (GRCm39)	S73P	probably damaging	Het
Mtdh	T	G	15: 34,116,473 (GRCm39)	N17K	probably damaging	Het
Muc3a	A	G	5: 137,246,591 (GRCm39)	S9P	probably benign	Het
Or8b46	A	G	9: 38,558,751 (GRCm39)	L23P	probably damaging	Het
Oxgr1	T	A	14: 120,259,419 (GRCm39)	I263F	probably benign	Het
Phc1	A	G	6: 122,311,923 (GRCm39)			Het
Ppp2r5d	A	G	17: 46,996,509 (GRCm39)	F388S	probably damaging	Het
Pxmp4	A	G	2: 154,434,083 (GRCm39)		probably null	Het
Rnd2	C	T	11: 101,359,825 (GRCm39)	L57F	probably damaging	Het
Ror1	A	G	4: 100,267,109 (GRCm39)	N270S	possibly damaging	Het
Slc7a14	T	C	3: 31,278,093 (GRCm39)	Y504C	probably damaging	Het
Snx9	A	G	17: 5,970,437 (GRCm39)	D391G	probably benign	Het
St18	T	A	1: 6,898,209 (GRCm39)	Y670*	probably null	Het
Tjp1	A	T	7: 64,986,865 (GRCm39)	F207I	possibly damaging	Het
Trappc3	A	G	4: 126,169,022 (GRCm39)	I171V	probably benign	Het
Ugt2b5	A	T	5: 87,273,328 (GRCm39)	L446*	probably null	Het
Vmn2r11	A	T	5: 109,196,800 (GRCm39)	N557K	possibly damaging	Het
Yod1	G	A	1: 130,645,275 (GRCm39)	G19S	probably damaging	Het
Zfp938	A	G	10: 82,063,363 (GRCm39)	*65Q	probably null	Het

Other mutations in Vmn2r105

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01433:Vmn2r105	APN	17	20,448,817 (GRCm39)	missense	probably benign	0.01
IGL01909:Vmn2r105	APN	17	20,444,918 (GRCm39)	missense	probably damaging	1.00
IGL01925:Vmn2r105	APN	17	20,428,973 (GRCm39)	missense	possibly damaging	0.94
IGL02021:Vmn2r105	APN	17	20,448,157 (GRCm39)	missense	possibly damaging	0.49
IGL02828:Vmn2r105	APN	17	20,429,345 (GRCm39)	missense	possibly damaging	0.80
IGL02838:Vmn2r105	APN	17	20,447,847 (GRCm39)	missense	probably damaging	1.00
IGL03343:Vmn2r105	APN	17	20,446,631 (GRCm39)	nonsense	probably null
R0096:Vmn2r105	UTSW	17	20,447,741 (GRCm39)	missense	possibly damaging	0.49
R0096:Vmn2r105	UTSW	17	20,447,741 (GRCm39)	missense	possibly damaging	0.49
R0212:Vmn2r105	UTSW	17	20,428,827 (GRCm39)	missense	possibly damaging	0.90
R0268:Vmn2r105	UTSW	17	20,428,938 (GRCm39)	missense	probably benign	0.18
R0271:Vmn2r105	UTSW	17	20,454,965 (GRCm39)	missense	probably damaging	0.96
R0613:Vmn2r105	UTSW	17	20,428,578 (GRCm39)	missense	probably damaging	1.00
R0765:Vmn2r105	UTSW	17	20,448,119 (GRCm39)	missense	probably damaging	0.98
R0765:Vmn2r105	UTSW	17	20,447,973 (GRCm39)	missense	probably benign	0.20
R1162:Vmn2r105	UTSW	17	20,447,973 (GRCm39)	missense	probably benign	0.20
R1263:Vmn2r105	UTSW	17	20,428,584 (GRCm39)	missense	probably damaging	1.00
R1363:Vmn2r105	UTSW	17	20,428,932 (GRCm39)	missense	probably benign	0.00
R1464:Vmn2r105	UTSW	17	20,449,004 (GRCm39)	splice site	probably benign
R2029:Vmn2r105	UTSW	17	20,444,840 (GRCm39)	missense	probably damaging	0.99
R2420:Vmn2r105	UTSW	17	20,448,097 (GRCm39)	missense	probably benign	0.15
R2421:Vmn2r105	UTSW	17	20,448,097 (GRCm39)	missense	probably benign	0.15
R2422:Vmn2r105	UTSW	17	20,448,097 (GRCm39)	missense	probably benign	0.15
R2570:Vmn2r105	UTSW	17	20,447,585 (GRCm39)	missense	probably damaging	1.00
R3847:Vmn2r105	UTSW	17	20,428,952 (GRCm39)	missense	possibly damaging	0.85
R3848:Vmn2r105	UTSW	17	20,428,952 (GRCm39)	missense	possibly damaging	0.85
R4030:Vmn2r105	UTSW	17	20,429,016 (GRCm39)	missense	probably damaging	0.99
R4275:Vmn2r105	UTSW	17	20,448,902 (GRCm39)	missense	probably damaging	1.00
R4551:Vmn2r105	UTSW	17	20,446,613 (GRCm39)	missense	probably benign
R4801:Vmn2r105	UTSW	17	20,447,556 (GRCm39)	missense	probably benign	0.00
R4802:Vmn2r105	UTSW	17	20,447,556 (GRCm39)	missense	probably benign	0.00
R4816:Vmn2r105	UTSW	17	20,428,953 (GRCm39)	missense	probably benign	0.27
R4929:Vmn2r105	UTSW	17	20,448,280 (GRCm39)	missense	probably benign	0.44
R5022:Vmn2r105	UTSW	17	20,428,676 (GRCm39)	missense	probably damaging	0.99
R5475:Vmn2r105	UTSW	17	20,455,044 (GRCm39)	missense	probably benign
R5576:Vmn2r105	UTSW	17	20,444,836 (GRCm39)	critical splice donor site	probably null
R5795:Vmn2r105	UTSW	17	20,448,998 (GRCm39)	missense	probably benign	0.00
R5895:Vmn2r105	UTSW	17	20,448,929 (GRCm39)	missense	probably benign	0.10
R6017:Vmn2r105	UTSW	17	20,428,889 (GRCm39)	missense	probably damaging	0.97
R6210:Vmn2r105	UTSW	17	20,448,758 (GRCm39)	missense	probably damaging	1.00
R6542:Vmn2r105	UTSW	17	20,448,803 (GRCm39)	missense	probably benign	0.03
R6729:Vmn2r105	UTSW	17	20,428,605 (GRCm39)	missense	probably damaging	0.99
R7020:Vmn2r105	UTSW	17	20,429,336 (GRCm39)	missense	probably damaging	1.00
R7033:Vmn2r105	UTSW	17	20,428,874 (GRCm39)	missense	probably damaging	0.97
R7488:Vmn2r105	UTSW	17	20,429,045 (GRCm39)	missense	probably damaging	1.00
R7491:Vmn2r105	UTSW	17	20,448,827 (GRCm39)	missense	probably benign	0.02
R7555:Vmn2r105	UTSW	17	20,447,937 (GRCm39)	missense	probably damaging	0.98
R7863:Vmn2r105	UTSW	17	20,428,937 (GRCm39)	missense	probably benign	0.18
R8137:Vmn2r105	UTSW	17	20,454,966 (GRCm39)	missense	probably benign	0.02
R8166:Vmn2r105	UTSW	17	20,428,904 (GRCm39)	missense	probably benign	0.07
R8186:Vmn2r105	UTSW	17	20,444,880 (GRCm39)	nonsense	probably null
R8214:Vmn2r105	UTSW	17	20,448,775 (GRCm39)	missense	probably benign	0.02
R8497:Vmn2r105	UTSW	17	20,455,134 (GRCm39)	start codon destroyed	probably null	0.75
R8850:Vmn2r105	UTSW	17	20,428,872 (GRCm39)	missense	probably damaging	1.00
R8880:Vmn2r105	UTSW	17	20,429,229 (GRCm39)	missense	probably damaging	0.99
R9272:Vmn2r105	UTSW	17	20,447,685 (GRCm39)	missense	probably damaging	1.00
R9506:Vmn2r105	UTSW	17	20,429,404 (GRCm39)	missense	probably benign	0.00
R9549:Vmn2r105	UTSW	17	20,448,023 (GRCm39)	missense	probably benign	0.12

Predicted Primers

PCR Primer
(F):5'- GGCAATGCATCCAAAGAAGC -3'
(R):5'- GGTCTCATTCTTCCAGATGACC -3'

Sequencing Primer
(F):5'- TGCATCCAAAGAAGCATTGAACTG -3'
(R):5'- TTCTTCCAGATGACCACAAAGG -3'

Posted On

2018-06-06