Mutagenetix > Incidental Mutation

Incidental Mutation 'PIT4131001:Cfap65'

523155

Institutional Source

Beutler Lab

Gene Symbol

Cfap65

Ensembl Gene

ENSMUSG00000047021

Gene Name

cilia and flagella associated protein 65

Synonyms

Ccdc108, B230363K08Rik

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.677) question?

Stock #

PIT4131001 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

74941230-74974758 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 74967501 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 192 (N192K)

Ref Sequence

ENSEMBL: ENSMUSP00000092440 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094844]

AlphaFold

Q3V0B4

Predicted Effect

probably benign
Transcript: ENSMUST00000094844
AA Change: N192K

PolyPhen 2 Score 0.045 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000092440
Gene: ENSMUSG00000047021
AA Change: N192K

Domain	Start	End	E-Value	Type
transmembrane domain	111	133	N/A	INTRINSIC
low complexity region	212	223	N/A	INTRINSIC
internal_repeat_1	745	890	9.31e-5	PROSPERO
internal_repeat_1	1167	1322	9.31e-5	PROSPERO
low complexity region	1350	1361	N/A	INTRINSIC
low complexity region	1574	1592	N/A	INTRINSIC
coiled coil region	1687	1724	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130489

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000139950

Coding Region Coverage

1x: 0.0%
3x: 0.0%
10x: 0.0%
20x: 0.0%

Validation Efficiency

92% (126/137)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene has putative coiled-coil domains and may be a transmembrane protein. The chicken ortholog of this gene is involved in the Rose-comb mutation, which is a large chromosome inversion, resulting in altered comb morphology and defects in sperm motility. [provided by RefSeq, Aug 2016]

Allele List at MGI

Other mutations in this stock

Total: 129 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700010B08Rik	C	T	2: 173,561,599 (GRCm39)		probably benign	Het
Alpk2	G	A	18: 65,439,450 (GRCm39)	H648Y	possibly damaging	Het
Ambp	A	G	4: 63,062,502 (GRCm39)	Y246H	probably damaging	Het
Amz1	T	C	5: 140,735,088 (GRCm39)		probably null	Het
Anks6	G	A	4: 47,027,109 (GRCm39)	T703I	probably damaging	Het
Armc2	A	G	10: 41,823,883 (GRCm39)		probably benign	Het
Atp7b	T	A	8: 22,484,672 (GRCm39)	I1347F	probably damaging	Het
Atp8a1	C	T	5: 67,779,945 (GRCm39)	W1149*	probably null	Het
Auh	A	G	13: 52,995,046 (GRCm39)	I173T	probably damaging	Het
Axin2	T	C	11: 108,814,829 (GRCm39)	L239P	possibly damaging	Het
Bbs1	A	T	19: 4,949,287 (GRCm39)	F257L	possibly damaging	Het
Cacna2d2	C	T	9: 107,401,867 (GRCm39)	P774L	probably damaging	Het
Card6	A	G	15: 5,137,788 (GRCm39)	L22P	probably damaging	Het
Ccdc171	C	T	4: 83,579,946 (GRCm39)			Het
Ccn2	T	C	10: 24,471,988 (GRCm39)	V70A	probably damaging	Het
Cdc14a	T	A	3: 116,122,310 (GRCm39)	N219I	possibly damaging	Het
Col14a1	T	C	15: 55,312,272 (GRCm39)		probably benign	Het
Col5a1	A	G	2: 27,914,665 (GRCm39)	T94A	probably benign	Het
Col6a5	T	C	9: 105,759,113 (GRCm39)	N2031S	probably damaging	Het
Col7a1	T	C	9: 108,794,989 (GRCm39)		probably benign	Het
Cyld	T	C	8: 89,473,543 (GRCm39)	S739P	probably damaging	Het
Dbr1	A	G	9: 99,466,072 (GRCm39)		probably null	Het
Dip2b	T	C	15: 100,100,233 (GRCm39)	L1267P	probably damaging	Het
Dolk	A	G	2: 30,175,586 (GRCm39)	M153T	probably benign	Het
Duxf1	C	T	10: 58,060,704 (GRCm39)	E17K	possibly damaging	Het
Duxf1	G	A	10: 58,060,136 (GRCm39)		probably benign	Het
Duxf1	A	G	10: 58,059,276 (GRCm39)	C493R	probably benign	Het
Duxf3	A	C	10: 58,067,498 (GRCm39)	S27A	probably benign	Het
Eef1d	C	T	15: 75,775,581 (GRCm39)	R26H	probably benign	Homo
Efcab5	C	T	11: 77,028,517 (GRCm39)			Het
Epc1	T	C	18: 6,449,246 (GRCm39)	D467G	probably damaging	Het
Fancm	T	G	12: 65,152,196 (GRCm39)	M884R	probably benign	Het
Fbxo24	G	T	5: 137,620,164 (GRCm39)	H15N	probably damaging	Het
Frem1	A	G	4: 82,924,045 (GRCm39)	F305L	probably damaging	Het
Fstl5	A	G	3: 76,567,006 (GRCm39)	D550G	probably damaging	Het
Gcnt3	T	G	9: 69,941,326 (GRCm39)	K414T	possibly damaging	Het
Gm10718	A	T	9: 3,024,417 (GRCm39)	T134S	probably benign	Het
Gm10722	A	G,C	9: 3,001,414 (GRCm39)		probably benign	Het
Gm10800	T	C	2: 98,497,163 (GRCm39)	R152G	probably benign	Homo
Gm10800	C	A	2: 98,497,250 (GRCm39)	V123F	probably benign	Homo
Gm10800	A	C	2: 98,496,893 (GRCm39)	F220C	probably benign	Het
Gm10801	A	G	2: 98,492,648 (GRCm39)	R23G	probably benign	Homo
Gm11168	C	T	9: 3,004,605 (GRCm39)	P49S	probably benign	Het
Gm21738	G	A	14: 19,417,330 (GRCm38)	S66L	probably benign	Het
Hjurp	TCTGGGAGGGCTTGCTCCGGGGGCAGTGTGTCCTGTTCTTGTGCAGCCCCTG	T	1: 88,194,000 (GRCm39)		probably benign	Het
Hmgcr	A	G	13: 96,795,562 (GRCm39)	Y336H	probably damaging	Het
Hoxa13	G	C	6: 52,260,648 (GRCm38)		probably benign	Homo
Hoxa13	C	G	6: 52,260,647 (GRCm38)		probably benign	Homo
Igf2bp3	A	C	6: 49,094,084 (GRCm39)		probably null	Het
Kcnb2	A	T	1: 15,383,200 (GRCm39)	K175N	possibly damaging	Het
Kdr	T	C	5: 76,102,631 (GRCm39)		probably benign	Het
Kif5c	A	G	2: 49,584,044 (GRCm39)	K160E	probably damaging	Het
Kif7	A	T	7: 79,360,817 (GRCm39)	V186E	probably damaging	Het
Krt16	T	A	11: 100,139,575 (GRCm39)	T48S	unknown	Het
Liph	T	C	16: 21,814,119 (GRCm39)	M1V	probably null	Het
Mctp2	A	G	7: 71,740,005 (GRCm39)	F795S	probably damaging	Het
Muc4	T	A	16: 32,755,699 (GRCm38)		probably benign	Homo
Muc4	C	G	16: 32,755,676 (GRCm38)		probably benign	Homo
Muc4	T	A	16: 32,755,684 (GRCm38)		probably benign	Homo
Myo15a	G	T	11: 60,373,953 (GRCm39)	A1267S	probably damaging	Het
Myo15a	T	C	11: 60,386,280 (GRCm39)	Y1802H	probably damaging	Het
Myo7b	G	A	18: 32,094,259 (GRCm39)	T1963I	probably benign	Het
Nadk2	TG	T	15: 9,100,232 (GRCm39)		probably null	Homo
Naip5	T	C	13: 100,356,268 (GRCm39)	N1116D	probably benign	Het
Naip5	T	C	13: 100,356,247 (GRCm39)	R1123G	probably benign	Het
Nap1l1	A	G	10: 111,322,583 (GRCm39)	D61G	probably null	Het
Napsa	A	T	7: 44,230,875 (GRCm39)	T81S	probably damaging	Het
Ngp	T	C	9: 110,251,337 (GRCm39)		probably benign	Het
Nktr	T	A	9: 121,570,687 (GRCm39)	V143E	probably damaging	Het
Obscn	A	G	11: 58,957,890 (GRCm39)		probably null	Het
Or10ag53	G	A	2: 87,082,973 (GRCm39)	A231T	probably benign	Het
Or11i1	T	C	3: 106,729,282 (GRCm39)	I198V	probably benign	Het
Or4f56	G	A	2: 111,703,649 (GRCm39)	L184F	probably benign	Het
Or52ab2	C	T	7: 102,970,076 (GRCm39)	R153*	probably null	Het
Or52d3	G	A	7: 104,229,237 (GRCm39)	R128Q	probably damaging	Het
Or6p1	A	G	1: 174,258,390 (GRCm39)	Y132C	probably damaging	Het
Paip2b	A	G	6: 83,785,823 (GRCm39)	Y136H	probably damaging	Het
Pde2a	G	T	7: 101,160,361 (GRCm39)	R845L	probably damaging	Het
Pgap2	A	G	7: 101,886,405 (GRCm39)	Y197C	possibly damaging	Het
Phf11b	A	G	14: 59,560,611 (GRCm39)		probably benign	Het
Pitpnm2	A	T	5: 124,269,178 (GRCm39)	D481E	probably benign	Het
Pxk	A	T	14: 8,152,130 (GRCm38)	H482L	probably benign	Het
Rad50	A	G	11: 53,585,726 (GRCm39)		probably null	Het
Rbmyf1	T	A	Y: 2,787,132 (GRCm39)	N228Y	probably benign	Het
Rbmyf5	T	C	Y: 3,297,411 (GRCm39)	H235R	probably benign	Het
Rbmyf6	C	T	Y: 3,328,944 (GRCm39)	A241T	possibly damaging	Het
Rnf220	A	G	4: 117,134,566 (GRCm39)		probably null	Het
Saxo5	T	A	8: 3,526,062 (GRCm39)	S72T	possibly damaging	Het
Selenbp1	C	T	3: 94,844,607 (GRCm39)	T88M	probably damaging	Het
Sft2d1	T	C	17: 8,609,863 (GRCm39)	I104T	possibly damaging	Het
Sik1	A	G	17: 32,070,305 (GRCm39)	S135P	probably damaging	Het
Slc16a3	T	C	11: 120,846,172 (GRCm39)	F34L	probably damaging	Het
Slc6a20b	T	C	9: 123,612,126 (GRCm38)	N85S	probably benign	Homo
Snrnp27	T	C	6: 86,659,893 (GRCm39)	R34G	unknown	Het
Sos2	T	C	12: 69,664,851 (GRCm39)	H393R	probably benign	Het
Sp110	C	T	1: 85,513,971 (GRCm39)	R262Q	probably benign	Het
Sp110	T	C	1: 85,513,975 (GRCm39)	R261G	probably benign	Het
Sp140	T	A	1: 85,528,893 (GRCm39)	Y5N	probably benign	Het
Sp140	A	G	1: 85,570,942 (GRCm39)	S461G	probably benign	Het
Sp140	G	C	1: 85,538,603 (GRCm39)	K113N	probably benign	Het
Sp140l1	G	A	1: 85,077,341 (GRCm39)	A75V	probably benign	Het
Sp140l2	A	C	1: 85,223,395 (GRCm39)		probably benign	Het
Speer4a2	A	T	5: 26,291,485 (GRCm39)	F107Y	probably benign	Het
Speer4a2	C	G	5: 26,294,093 (GRCm39)	W28C	probably damaging	Het
Ssrp1	G	A	2: 84,868,760 (GRCm39)	V40M	probably damaging	Het
Tada2a	T	C	11: 83,970,563 (GRCm39)	E202G	probably damaging	Het
Tcf20	C	A	15: 82,735,785 (GRCm39)	A1889S	probably damaging	Het
Tdrd12	A	T	7: 35,180,528 (GRCm39)	Y828*	probably null	Het
Tlr2	T	A	3: 83,745,756 (GRCm39)	D109V	probably benign	Het
Tomm40	G	A	7: 19,437,016 (GRCm39)	T17M	probably damaging	Het
Tsga10ip	T	C	19: 5,440,161 (GRCm39)	T135A	possibly damaging	Het
Tspan8	G	A	10: 115,653,515 (GRCm39)	V4M	probably damaging	Het
Ttc28	A	T	5: 111,040,719 (GRCm39)	T36S	probably benign	Het
Ugt1a6b	G	A	1: 88,146,112 (GRCm39)	R519Q	probably damaging	Het
Ugt1a6b	TTCA	T	1: 88,143,880 (GRCm39)		probably benign	Het
Ugt1a6b	G	A	1: 88,143,976 (GRCm39)	A199T	probably damaging	Het
Unc45a	A	G	7: 79,976,109 (GRCm39)	M790T	possibly damaging	Het
Vav3	T	C	3: 109,571,751 (GRCm39)		probably null	Het
Vcpkmt	G	A	12: 69,629,552 (GRCm39)	S70L	probably benign	Het
Vmn1r3	C	T	4: 3,184,691 (GRCm39)	M205I	probably damaging	Het
Vmn1r3	C	T	4: 3,184,774 (GRCm39)	V178I	probably benign	Het
Vmn2r66	T	C	7: 84,644,301 (GRCm39)	Q703R	probably damaging	Het
Vmn2r98	G	T	17: 19,301,223 (GRCm39)	V742F	probably benign	Het
Wfdc8	A	G	2: 164,439,696 (GRCm39)	S229P	possibly damaging	Het
Xpo4	A	T	14: 57,822,068 (GRCm39)	C1083S	probably null	Het
Zbtb38	T	C	9: 96,568,369 (GRCm39)	D905G	probably damaging	Het
Zbtb8b	A	G	4: 129,321,308 (GRCm39)	*518Q	probably null	Het
Zfp600	TC	T	4: 146,131,802 (GRCm39)		probably null	Het
Zfp992	C	T	4: 146,550,569 (GRCm39)	P97S	probably benign	Het

Other mutations in Cfap65

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01107:Cfap65	APN	1	74,958,342 (GRCm39)	critical splice donor site	probably null
IGL01526:Cfap65	APN	1	74,950,237 (GRCm39)	missense	probably damaging	1.00
IGL01716:Cfap65	APN	1	74,966,353 (GRCm39)	missense	probably benign
IGL01780:Cfap65	APN	1	74,967,507 (GRCm39)	nonsense	probably null
IGL01993:Cfap65	APN	1	74,959,702 (GRCm39)	missense	probably damaging	1.00
IGL02164:Cfap65	APN	1	74,967,304 (GRCm39)	missense	possibly damaging	0.87
IGL02350:Cfap65	APN	1	74,967,507 (GRCm39)	nonsense	probably null
IGL02357:Cfap65	APN	1	74,967,507 (GRCm39)	nonsense	probably null
IGL02576:Cfap65	APN	1	74,942,617 (GRCm39)	missense	probably damaging	1.00
IGL02756:Cfap65	APN	1	74,944,239 (GRCm39)	missense	probably benign	0.00
IGL02792:Cfap65	APN	1	74,966,337 (GRCm39)	missense	probably damaging	1.00
IGL02874:Cfap65	APN	1	74,950,267 (GRCm39)	nonsense	probably null
IGL03101:Cfap65	APN	1	74,967,592 (GRCm39)	missense	possibly damaging	0.61
IGL03348:Cfap65	APN	1	74,966,778 (GRCm39)	missense	probably damaging	1.00
IGL03396:Cfap65	APN	1	74,943,801 (GRCm39)	missense	probably damaging	1.00
R0077:Cfap65	UTSW	1	74,971,077 (GRCm39)	missense	probably damaging	1.00
R0227:Cfap65	UTSW	1	74,971,117 (GRCm39)	nonsense	probably null
R0281:Cfap65	UTSW	1	74,966,230 (GRCm39)	missense	probably damaging	1.00
R0312:Cfap65	UTSW	1	74,943,226 (GRCm39)	missense	probably damaging	1.00
R0331:Cfap65	UTSW	1	74,968,461 (GRCm39)	missense	probably damaging	1.00
R0331:Cfap65	UTSW	1	74,968,460 (GRCm39)	missense	probably damaging	1.00
R0347:Cfap65	UTSW	1	74,965,603 (GRCm39)	missense	probably damaging	1.00
R0359:Cfap65	UTSW	1	74,959,760 (GRCm39)	missense	probably benign	0.00
R0361:Cfap65	UTSW	1	74,964,599 (GRCm39)	missense	probably damaging	1.00
R0465:Cfap65	UTSW	1	74,956,043 (GRCm39)	missense	possibly damaging	0.92
R0549:Cfap65	UTSW	1	74,957,603 (GRCm39)	missense	probably benign	0.01
R0646:Cfap65	UTSW	1	74,941,328 (GRCm39)	missense	probably benign	0.09
R0734:Cfap65	UTSW	1	74,958,046 (GRCm39)	missense	probably damaging	1.00
R0763:Cfap65	UTSW	1	74,943,841 (GRCm39)	missense	probably damaging	0.99
R0990:Cfap65	UTSW	1	74,960,678 (GRCm39)	missense	possibly damaging	0.60
R1079:Cfap65	UTSW	1	74,941,606 (GRCm39)	missense	probably damaging	0.98
R1079:Cfap65	UTSW	1	74,944,872 (GRCm39)	missense	probably damaging	0.99
R1083:Cfap65	UTSW	1	74,957,663 (GRCm39)	splice site	probably benign
R1159:Cfap65	UTSW	1	74,968,499 (GRCm39)	missense	probably damaging	1.00
R1282:Cfap65	UTSW	1	74,964,263 (GRCm39)	missense	probably benign	0.03
R1644:Cfap65	UTSW	1	74,956,334 (GRCm39)	missense	probably damaging	1.00
R1796:Cfap65	UTSW	1	74,958,107 (GRCm39)	missense	probably damaging	1.00
R1950:Cfap65	UTSW	1	74,946,819 (GRCm39)	missense	probably damaging	1.00
R2079:Cfap65	UTSW	1	74,956,358 (GRCm39)	missense	probably benign	0.30
R2132:Cfap65	UTSW	1	74,946,850 (GRCm39)	missense	probably damaging	1.00
R2136:Cfap65	UTSW	1	74,956,432 (GRCm39)	frame shift	probably null
R2219:Cfap65	UTSW	1	74,943,184 (GRCm39)	missense	probably damaging	1.00
R2220:Cfap65	UTSW	1	74,943,184 (GRCm39)	missense	probably damaging	1.00
R2291:Cfap65	UTSW	1	74,965,634 (GRCm39)	missense	probably damaging	1.00
R2417:Cfap65	UTSW	1	74,966,345 (GRCm39)	small insertion	probably benign
R3114:Cfap65	UTSW	1	74,966,291 (GRCm39)	missense	probably damaging	1.00
R4202:Cfap65	UTSW	1	74,959,701 (GRCm39)	missense	probably damaging	1.00
R4214:Cfap65	UTSW	1	74,966,840 (GRCm39)	missense	possibly damaging	0.93
R4254:Cfap65	UTSW	1	74,942,517 (GRCm39)	missense	probably benign	0.17
R4547:Cfap65	UTSW	1	74,946,771 (GRCm39)	missense	probably damaging	1.00
R4548:Cfap65	UTSW	1	74,946,771 (GRCm39)	missense	probably damaging	1.00
R4588:Cfap65	UTSW	1	74,943,215 (GRCm39)	missense	possibly damaging	0.92
R4657:Cfap65	UTSW	1	74,964,513 (GRCm39)	intron	probably benign
R4701:Cfap65	UTSW	1	74,958,067 (GRCm39)	missense	probably damaging	0.96
R4755:Cfap65	UTSW	1	74,967,520 (GRCm39)	missense	probably damaging	1.00
R4820:Cfap65	UTSW	1	74,966,791 (GRCm39)	missense	probably benign	0.06
R4831:Cfap65	UTSW	1	74,956,454 (GRCm39)	missense	possibly damaging	0.93
R4866:Cfap65	UTSW	1	74,964,716 (GRCm39)	missense	probably damaging	1.00
R4869:Cfap65	UTSW	1	74,958,420 (GRCm39)	missense	probably benign	0.00
R4881:Cfap65	UTSW	1	74,946,772 (GRCm39)	missense	probably damaging	1.00
R4884:Cfap65	UTSW	1	74,942,283 (GRCm39)	missense	possibly damaging	0.47
R4950:Cfap65	UTSW	1	74,945,495 (GRCm39)	nonsense	probably null
R5074:Cfap65	UTSW	1	74,962,137 (GRCm39)	missense	probably benign	0.04
R5083:Cfap65	UTSW	1	74,945,600 (GRCm39)	missense	probably damaging	1.00
R5164:Cfap65	UTSW	1	74,965,675 (GRCm39)	missense	probably damaging	1.00
R5268:Cfap65	UTSW	1	74,964,061 (GRCm39)	missense	probably benign	0.07
R5333:Cfap65	UTSW	1	74,942,334 (GRCm39)	missense	probably benign	0.03
R5417:Cfap65	UTSW	1	74,964,259 (GRCm39)	missense	probably damaging	1.00
R5582:Cfap65	UTSW	1	74,946,677 (GRCm39)	intron	probably benign
R5669:Cfap65	UTSW	1	74,964,127 (GRCm39)	missense	probably damaging	0.99
R6010:Cfap65	UTSW	1	74,962,190 (GRCm39)	missense	probably damaging	1.00
R6084:Cfap65	UTSW	1	74,959,564 (GRCm39)	missense	probably damaging	1.00
R6112:Cfap65	UTSW	1	74,942,298 (GRCm39)	missense	probably benign	0.14
R6425:Cfap65	UTSW	1	74,966,868 (GRCm39)	missense	probably benign	0.00
R6677:Cfap65	UTSW	1	74,943,844 (GRCm39)	missense	probably damaging	1.00
R6693:Cfap65	UTSW	1	74,956,445 (GRCm39)	missense	probably benign	0.00
R6838:Cfap65	UTSW	1	74,971,180 (GRCm39)	missense	probably benign	0.06
R6861:Cfap65	UTSW	1	74,964,274 (GRCm39)	missense	probably damaging	1.00
R6958:Cfap65	UTSW	1	74,971,058 (GRCm39)	missense	possibly damaging	0.58
R7134:Cfap65	UTSW	1	74,965,792 (GRCm39)	missense	probably benign	0.01
R7320:Cfap65	UTSW	1	74,965,763 (GRCm39)	missense	probably damaging	0.99
R7340:Cfap65	UTSW	1	74,960,742 (GRCm39)	missense	probably benign	0.07
R7426:Cfap65	UTSW	1	74,959,585 (GRCm39)	missense	possibly damaging	0.92
R7529:Cfap65	UTSW	1	74,965,769 (GRCm39)	missense	probably damaging	1.00
R7634:Cfap65	UTSW	1	74,941,593 (GRCm39)	missense	probably damaging	1.00
R7654:Cfap65	UTSW	1	74,972,303 (GRCm39)	missense	probably benign	0.44
R7704:Cfap65	UTSW	1	74,967,527 (GRCm39)	missense	probably benign	0.19
R7727:Cfap65	UTSW	1	74,965,784 (GRCm39)	missense	probably benign	0.00
R7895:Cfap65	UTSW	1	74,972,321 (GRCm39)	missense	probably benign	0.05
R8215:Cfap65	UTSW	1	74,949,902 (GRCm39)	missense	probably damaging	1.00
R8344:Cfap65	UTSW	1	74,967,203 (GRCm39)	missense	probably benign	0.01
R8345:Cfap65	UTSW	1	74,967,203 (GRCm39)	missense	probably benign	0.01
R8413:Cfap65	UTSW	1	74,956,328 (GRCm39)	nonsense	probably null
R8431:Cfap65	UTSW	1	74,967,203 (GRCm39)	missense	probably benign	0.01
R8432:Cfap65	UTSW	1	74,967,203 (GRCm39)	missense	probably benign	0.01
R8528:Cfap65	UTSW	1	74,945,096 (GRCm39)	missense	possibly damaging	0.88
R8809:Cfap65	UTSW	1	74,942,382 (GRCm39)	missense	probably benign	0.43
R8996:Cfap65	UTSW	1	74,941,347 (GRCm39)	missense	probably benign	0.11
R9020:Cfap65	UTSW	1	74,959,552 (GRCm39)	missense	probably damaging	1.00
R9043:Cfap65	UTSW	1	74,943,847 (GRCm39)	missense	possibly damaging	0.88
R9127:Cfap65	UTSW	1	74,958,510 (GRCm39)	splice site	probably benign
R9187:Cfap65	UTSW	1	74,956,517 (GRCm39)	missense	probably benign	0.00
R9210:Cfap65	UTSW	1	74,959,567 (GRCm39)	missense	probably benign
R9212:Cfap65	UTSW	1	74,959,567 (GRCm39)	missense	probably benign
R9273:Cfap65	UTSW	1	74,960,769 (GRCm39)	missense	probably benign	0.00
R9454:Cfap65	UTSW	1	74,944,210 (GRCm39)	missense	probably damaging	1.00
R9514:Cfap65	UTSW	1	74,945,468 (GRCm39)	critical splice donor site	probably null
R9595:Cfap65	UTSW	1	74,946,537 (GRCm39)	missense	probably damaging	1.00
R9721:Cfap65	UTSW	1	74,958,501 (GRCm39)	missense	probably benign	0.16
R9742:Cfap65	UTSW	1	74,943,840 (GRCm39)	missense	probably benign	0.08
RF009:Cfap65	UTSW	1	74,944,806 (GRCm39)	missense	probably damaging	1.00
Z1176:Cfap65	UTSW	1	74,949,906 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACTTCCCAGGTGAAGAAGGTG -3'
(R):5'- AAGTGGAATGTCCTGACTCTTACC -3'

Sequencing Primer
(F):5'- TGAAGAAGGTGGGCAGGTCC -3'
(R):5'- GGAATGTCCTGACTCTTACCTGAAC -3'

Posted On

2018-06-12