Mutagenetix > Incidental Mutation

Incidental Mutation 'R6582:Tas2r110'

524146

Institutional Source

Beutler Lab

Gene Symbol

Tas2r110

Ensembl Gene

ENSMUSG00000062952

Gene Name

taste receptor, type 2, member 110

Synonyms

T2R10, mt2r57, Tas2r10, STC 9-1

MMRRC Submission

044706-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.047) question?

Stock #

R6582 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

132844971-132845972 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 132845248 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 93 (I93N)

Ref Sequence

ENSEMBL: ENSMUSP00000080674 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000082014]

AlphaFold

Q7M712

Predicted Effect

possibly damaging
Transcript: ENSMUST00000082014
AA Change: I93N

PolyPhen 2 Score 0.935 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000080674
Gene: ENSMUSG00000062952
AA Change: I93N

Domain	Start	End	E-Value	Type
Pfam:TAS2R	6	322	3.1e-82	PFAM

Meta Mutation Damage Score

0.3181

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.0%
20x: 94.1%

Validation Efficiency

100% (40/40)

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca12	T	C	1: 71,297,384 (GRCm39)	T2369A	probably benign	Het
Abhd6	T	G	14: 8,042,828 (GRCm38)		probably null	Het
Abhd6	G	T	14: 8,042,826 (GRCm38)	G128C	probably damaging	Het
Acsm3	A	G	7: 119,378,896 (GRCm39)	E426G	probably benign	Het
Ankrd11	T	C	8: 123,618,368 (GRCm39)	D1828G	probably benign	Het
Asmt	T	A	X: 169,108,766 (GRCm39)		probably null	Het
Casp4	A	G	9: 5,324,884 (GRCm39)	Q232R	probably benign	Het
Cenpt	A	T	8: 106,575,833 (GRCm39)	L171*	probably null	Het
Chd3	AGCGGCGGCGGCGGCGGCGG	AGCGGCGGCGGCGGCGG	11: 69,259,982 (GRCm39)		probably benign	Het
Col5a2	G	T	1: 45,429,275 (GRCm39)	H948N	possibly damaging	Het
Dnah9	G	T	11: 65,951,923 (GRCm39)	H1859N	probably damaging	Het
Dscaml1	G	A	9: 45,664,104 (GRCm39)	R1993Q	probably benign	Het
Fbxw8	C	A	5: 118,263,028 (GRCm39)	R217L	probably benign	Het
Flnb	T	G	14: 7,892,275 (GRCm38)		probably null	Het
Fyb2	A	G	4: 104,802,739 (GRCm39)	N214D	probably benign	Het
Gbp2b	A	G	3: 142,316,801 (GRCm39)	E484G	possibly damaging	Het
Gzmk	A	G	13: 113,317,045 (GRCm39)	Y45H	probably damaging	Het
Ivl	CCTGCTGCTGCTGCT	CCTGCTGCTGCT	3: 92,479,217 (GRCm39)		probably benign	Het
Kcnj6	A	G	16: 94,633,685 (GRCm39)	V142A	possibly damaging	Het
Klri2	A	T	6: 129,716,096 (GRCm39)	I81K	possibly damaging	Het
Lama3	T	A	18: 12,710,897 (GRCm39)	V3144E	probably damaging	Het
Mark1	G	A	1: 184,644,786 (GRCm39)	S390L	possibly damaging	Het
Mbd3l1	T	C	9: 18,396,024 (GRCm39)	Y50H	probably benign	Het
Mcat	T	C	15: 83,433,383 (GRCm39)	N220S	probably benign	Het
Muc2	A	G	7: 141,282,941 (GRCm39)	E81G	probably benign	Het
Neto1	A	T	18: 86,512,985 (GRCm39)	K327*	probably null	Het
Or10j3	G	T	1: 173,031,847 (GRCm39)	R308L	probably benign	Het
Or4a67	A	T	2: 88,598,587 (GRCm39)	L24Q	probably damaging	Het
Or52p1	A	T	7: 104,267,648 (GRCm39)	Y254F	probably damaging	Het
Or52u1	T	C	7: 104,237,218 (GRCm39)	L69P	probably damaging	Het
Pidd1	A	T	7: 141,019,494 (GRCm39)	V722D	probably damaging	Het
Ppp2r2a	G	T	14: 67,257,253 (GRCm39)	H326N	probably damaging	Het
Smarca5	G	A	8: 81,446,281 (GRCm39)	T473I	probably damaging	Het
Spg11	C	A	2: 121,922,773 (GRCm39)	W892L	probably damaging	Het
Tiam2	CGGG	CGGGG	17: 3,464,897 (GRCm39)		probably null	Het
Vmn1r71	T	A	7: 10,482,608 (GRCm39)	I27F	probably benign	Het
Vsnl1	A	G	12: 11,376,489 (GRCm39)	V132A	probably benign	Het
Wdsub1	T	C	2: 59,708,652 (GRCm39)	T74A	probably damaging	Het
Ywhaz	T	C	15: 36,791,166 (GRCm39)	Y19C	probably damaging	Het

Other mutations in Tas2r110

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03103:Tas2r110	APN	6	132,845,443 (GRCm39)	missense	probably benign	0.09
IGL03275:Tas2r110	APN	6	132,845,061 (GRCm39)	missense	probably damaging	0.99
R0111:Tas2r110	UTSW	6	132,845,166 (GRCm39)	missense	probably benign	0.00
R0539:Tas2r110	UTSW	6	132,845,334 (GRCm39)	missense	possibly damaging	0.63
R1432:Tas2r110	UTSW	6	132,845,331 (GRCm39)	missense	probably damaging	1.00
R1672:Tas2r110	UTSW	6	132,845,029 (GRCm39)	missense	probably damaging	1.00
R2483:Tas2r110	UTSW	6	132,845,433 (GRCm39)	missense	probably benign	0.00
R3110:Tas2r110	UTSW	6	132,844,987 (GRCm39)	missense	unknown
R3112:Tas2r110	UTSW	6	132,844,987 (GRCm39)	missense	unknown
R3623:Tas2r110	UTSW	6	132,845,433 (GRCm39)	missense	probably benign	0.00
R3847:Tas2r110	UTSW	6	132,845,638 (GRCm39)	missense	probably damaging	1.00
R3849:Tas2r110	UTSW	6	132,845,638 (GRCm39)	missense	probably damaging	1.00
R3850:Tas2r110	UTSW	6	132,845,638 (GRCm39)	missense	probably damaging	1.00
R4871:Tas2r110	UTSW	6	132,845,091 (GRCm39)	missense	probably benign	0.09
R5010:Tas2r110	UTSW	6	132,845,438 (GRCm39)	nonsense	probably null
R5108:Tas2r110	UTSW	6	132,845,668 (GRCm39)	missense	probably damaging	1.00
R5289:Tas2r110	UTSW	6	132,844,972 (GRCm39)	start codon destroyed	probably null
R5938:Tas2r110	UTSW	6	132,845,016 (GRCm39)	missense	probably benign	0.39
R6262:Tas2r110	UTSW	6	132,845,638 (GRCm39)	missense	probably damaging	0.96
R6286:Tas2r110	UTSW	6	132,845,490 (GRCm39)	missense	probably benign	0.01
R7236:Tas2r110	UTSW	6	132,845,667 (GRCm39)	missense	possibly damaging	0.76
R8897:Tas2r110	UTSW	6	132,845,374 (GRCm39)	missense	probably damaging	1.00
X0024:Tas2r110	UTSW	6	132,845,596 (GRCm39)	missense	probably damaging	1.00
Z1176:Tas2r110	UTSW	6	132,845,574 (GRCm39)	missense	probably benign	0.37

Predicted Primers

PCR Primer
(F):5'- TGAATATCATGGACTGGACCAAG -3'
(R):5'- TGGGAAATGGGGTTGAATCC -3'

Sequencing Primer
(F):5'- TGGACCAAGAGAAGAAGCATTTCATC -3'
(R):5'- GAGAACTGATCATACATGCTTAGAG -3'

Posted On

2018-06-22