Incidental Mutation 'R6585:Gpr155'
| ID |
524318 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Gpr155
|
| Ensembl Gene |
ENSMUSG00000041762 |
| Gene Name |
G protein-coupled receptor 155 |
| Synonyms |
DEPDC3, 1110017O10Rik, PGR22, F730029F15Rik |
| MMRRC Submission |
044709-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R6585 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
2 |
| Chromosomal Location |
73171850-73216842 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to T
at 73179989 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Isoleucine to Asparagine
at position 157
(I157N)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000076463]
[ENSMUST00000112043]
[ENSMUST00000112044]
|
| AlphaFold |
A2AWR3 |
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000048504
|
| SMART Domains |
Protein: ENSMUSP00000037772
Gene: ENSMUSG00000041762
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Mem_trans
|
40 |
205 |
1.9e-16 |
PFAM |
|
Pfam:Mem_trans
|
187 |
360 |
4.9e-12 |
PFAM |
|
transmembrane domain
|
380 |
402 |
N/A |
INTRINSIC |
|
transmembrane domain
|
415 |
432 |
N/A |
INTRINSIC |
|
transmembrane domain
|
442 |
464 |
N/A |
INTRINSIC |
|
transmembrane domain
|
477 |
499 |
N/A |
INTRINSIC |
|
transmembrane domain
|
519 |
538 |
N/A |
INTRINSIC |
|
transmembrane domain
|
657 |
679 |
N/A |
INTRINSIC |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000076463
AA Change: I717N
PolyPhen 2
Score 0.948 (Sensitivity: 0.79; Specificity: 0.95)
|
| SMART Domains |
Protein: ENSMUSP00000075788
Gene: ENSMUSG00000041762
AA Change: I717N
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Mem_trans
|
40 |
205 |
3.3e-15 |
PFAM |
|
transmembrane domain
|
209 |
231 |
N/A |
INTRINSIC |
|
transmembrane domain
|
241 |
260 |
N/A |
INTRINSIC |
|
transmembrane domain
|
272 |
291 |
N/A |
INTRINSIC |
|
transmembrane domain
|
311 |
330 |
N/A |
INTRINSIC |
|
transmembrane domain
|
343 |
365 |
N/A |
INTRINSIC |
|
transmembrane domain
|
380 |
402 |
N/A |
INTRINSIC |
|
transmembrane domain
|
415 |
432 |
N/A |
INTRINSIC |
|
transmembrane domain
|
442 |
464 |
N/A |
INTRINSIC |
|
transmembrane domain
|
477 |
499 |
N/A |
INTRINSIC |
|
transmembrane domain
|
519 |
538 |
N/A |
INTRINSIC |
|
transmembrane domain
|
657 |
679 |
N/A |
INTRINSIC |
|
transmembrane domain
|
689 |
711 |
N/A |
INTRINSIC |
|
DEP
|
759 |
833 |
8.28e-12 |
SMART |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000112043
AA Change: I717N
PolyPhen 2
Score 0.948 (Sensitivity: 0.79; Specificity: 0.95)
|
| SMART Domains |
Protein: ENSMUSP00000107674
Gene: ENSMUSG00000041762
AA Change: I717N
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Mem_trans
|
40 |
205 |
3.3e-15 |
PFAM |
|
transmembrane domain
|
209 |
231 |
N/A |
INTRINSIC |
|
transmembrane domain
|
241 |
260 |
N/A |
INTRINSIC |
|
transmembrane domain
|
272 |
291 |
N/A |
INTRINSIC |
|
transmembrane domain
|
311 |
330 |
N/A |
INTRINSIC |
|
transmembrane domain
|
343 |
365 |
N/A |
INTRINSIC |
|
transmembrane domain
|
380 |
402 |
N/A |
INTRINSIC |
|
transmembrane domain
|
415 |
432 |
N/A |
INTRINSIC |
|
transmembrane domain
|
442 |
464 |
N/A |
INTRINSIC |
|
transmembrane domain
|
477 |
499 |
N/A |
INTRINSIC |
|
transmembrane domain
|
519 |
538 |
N/A |
INTRINSIC |
|
transmembrane domain
|
657 |
679 |
N/A |
INTRINSIC |
|
transmembrane domain
|
689 |
711 |
N/A |
INTRINSIC |
|
DEP
|
759 |
833 |
8.28e-12 |
SMART |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000112044
AA Change: I689N
PolyPhen 2
Score 0.948 (Sensitivity: 0.79; Specificity: 0.95)
|
| SMART Domains |
Protein: ENSMUSP00000107675
Gene: ENSMUSG00000041762
AA Change: I689N
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Mem_trans
|
40 |
205 |
3.3e-15 |
PFAM |
|
Pfam:Mem_trans
|
187 |
360 |
8.3e-11 |
PFAM |
|
transmembrane domain
|
382 |
404 |
N/A |
INTRINSIC |
|
transmembrane domain
|
414 |
436 |
N/A |
INTRINSIC |
|
transmembrane domain
|
449 |
471 |
N/A |
INTRINSIC |
|
transmembrane domain
|
491 |
510 |
N/A |
INTRINSIC |
|
transmembrane domain
|
629 |
651 |
N/A |
INTRINSIC |
|
transmembrane domain
|
661 |
683 |
N/A |
INTRINSIC |
|
DEP
|
731 |
805 |
8.28e-12 |
SMART |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000131430
AA Change: I157N
PolyPhen 2
Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
|
| SMART Domains |
Protein: ENSMUSP00000114294
Gene: ENSMUSG00000041762
AA Change: I157N
| Domain | Start | End | E-Value | Type |
|---|
|
transmembrane domain
|
98 |
120 |
N/A |
INTRINSIC |
|
transmembrane domain
|
130 |
152 |
N/A |
INTRINSIC |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000184503
|
| Meta Mutation Damage Score |
0.5703 |
| Coding Region Coverage |
- 1x: 99.9%
- 3x: 99.6%
- 10x: 98.1%
- 20x: 94.4%
|
| Validation Efficiency |
97% (30/31) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 30 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4930522L14Rik |
C |
T |
5: 109,885,534 (GRCm39) |
C108Y |
probably damaging |
Het |
| Adam1b |
G |
T |
5: 121,639,250 (GRCm39) |
D598E |
probably benign |
Het |
| Agr2 |
G |
A |
12: 36,045,625 (GRCm39) |
R37Q |
probably benign |
Het |
| Ascc3 |
A |
G |
10: 50,718,273 (GRCm39) |
K1989E |
probably benign |
Het |
| Chd1l |
A |
G |
3: 97,505,088 (GRCm39) |
F160L |
probably damaging |
Het |
| Ciita |
T |
A |
16: 10,329,609 (GRCm39) |
V628E |
probably benign |
Het |
| Defa38 |
A |
G |
8: 21,585,248 (GRCm39) |
C65R |
possibly damaging |
Het |
| Dis3l2 |
T |
A |
1: 86,673,216 (GRCm39) |
I69N |
probably damaging |
Het |
| Dnase1l1 |
C |
T |
X: 73,320,644 (GRCm39) |
|
probably null |
Homo |
| Elp2 |
T |
C |
18: 24,758,606 (GRCm39) |
L503S |
probably damaging |
Het |
| Fcgbp |
A |
T |
7: 27,813,404 (GRCm39) |
Q2313L |
possibly damaging |
Het |
| H2bc18 |
A |
T |
3: 96,177,413 (GRCm39) |
T116S |
probably benign |
Het |
| Kcnj1 |
T |
C |
9: 32,308,557 (GRCm39) |
V307A |
probably benign |
Het |
| Lama3 |
G |
A |
18: 12,552,314 (GRCm39) |
|
probably null |
Het |
| Lrp6 |
A |
T |
6: 134,484,521 (GRCm39) |
Y367* |
probably null |
Het |
| Ms4a14 |
T |
A |
19: 11,281,009 (GRCm39) |
Q516H |
unknown |
Het |
| Nprl3 |
C |
T |
11: 32,184,812 (GRCm39) |
R399Q |
probably benign |
Het |
| Or13c3 |
A |
T |
4: 52,856,192 (GRCm39) |
M107K |
possibly damaging |
Het |
| Or5ae1 |
A |
T |
7: 84,565,670 (GRCm39) |
I228F |
probably damaging |
Het |
| Park7 |
G |
T |
4: 150,989,721 (GRCm39) |
Q80K |
probably benign |
Het |
| Pramel15 |
C |
A |
4: 144,103,600 (GRCm39) |
L175F |
possibly damaging |
Het |
| Pramel52-ps |
C |
T |
5: 94,529,415 (GRCm39) |
P62S |
probably benign |
Het |
| Ptgs2 |
T |
C |
1: 149,979,738 (GRCm39) |
V281A |
possibly damaging |
Het |
| Rprd1a |
T |
C |
18: 24,639,720 (GRCm39) |
|
probably null |
Het |
| Speer4f2 |
A |
G |
5: 17,579,420 (GRCm39) |
E73G |
probably damaging |
Het |
| Spta1 |
T |
C |
1: 174,006,251 (GRCm39) |
W138R |
probably damaging |
Het |
| U2surp |
T |
C |
9: 95,354,124 (GRCm39) |
E838G |
probably damaging |
Het |
| Urb2 |
G |
T |
8: 124,757,864 (GRCm39) |
E1190D |
probably damaging |
Het |
| Usp19 |
G |
T |
9: 108,376,926 (GRCm39) |
L1165F |
probably damaging |
Het |
| Zfp27 |
G |
A |
7: 29,595,818 (GRCm39) |
T49I |
possibly damaging |
Het |
|
| Other mutations in Gpr155 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00731:Gpr155
|
APN |
2 |
73,192,957 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01432:Gpr155
|
APN |
2 |
73,182,229 (GRCm39) |
missense |
possibly damaging |
0.51 |
|
IGL01528:Gpr155
|
APN |
2 |
73,192,767 (GRCm39) |
critical splice donor site |
probably null |
|
|
IGL01718:Gpr155
|
APN |
2 |
73,212,576 (GRCm39) |
missense |
probably benign |
|
|
IGL01733:Gpr155
|
APN |
2 |
73,183,956 (GRCm39) |
splice site |
probably null |
|
|
IGL03342:Gpr155
|
APN |
2 |
73,180,022 (GRCm39) |
missense |
probably damaging |
1.00 |
|
PIT1430001:Gpr155
|
UTSW |
2 |
73,200,482 (GRCm39) |
missense |
probably benign |
0.08 |
|
PIT4810001:Gpr155
|
UTSW |
2 |
73,178,607 (GRCm39) |
missense |
probably benign |
0.08 |
|
R0226:Gpr155
|
UTSW |
2 |
73,197,936 (GRCm39) |
missense |
probably benign |
0.02 |
|
R0399:Gpr155
|
UTSW |
2 |
73,200,346 (GRCm39) |
missense |
possibly damaging |
0.91 |
|
R0445:Gpr155
|
UTSW |
2 |
73,200,488 (GRCm39) |
splice site |
probably benign |
|
|
R1570:Gpr155
|
UTSW |
2 |
73,200,382 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
R1598:Gpr155
|
UTSW |
2 |
73,200,434 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R1647:Gpr155
|
UTSW |
2 |
73,194,508 (GRCm39) |
splice site |
probably null |
|
|
R1648:Gpr155
|
UTSW |
2 |
73,194,508 (GRCm39) |
splice site |
probably null |
|
|
R1756:Gpr155
|
UTSW |
2 |
73,197,921 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1760:Gpr155
|
UTSW |
2 |
73,212,279 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2033:Gpr155
|
UTSW |
2 |
73,178,526 (GRCm39) |
missense |
probably benign |
0.18 |
|
R2044:Gpr155
|
UTSW |
2 |
73,203,977 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2145:Gpr155
|
UTSW |
2 |
73,187,002 (GRCm39) |
missense |
probably benign |
0.01 |
|
R2172:Gpr155
|
UTSW |
2 |
73,212,471 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2274:Gpr155
|
UTSW |
2 |
73,178,479 (GRCm39) |
critical splice donor site |
probably null |
|
|
R3878:Gpr155
|
UTSW |
2 |
73,198,736 (GRCm39) |
nonsense |
probably null |
|
|
R3924:Gpr155
|
UTSW |
2 |
73,200,420 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4910:Gpr155
|
UTSW |
2 |
73,197,882 (GRCm39) |
nonsense |
probably null |
|
|
R4950:Gpr155
|
UTSW |
2 |
73,212,529 (GRCm39) |
missense |
probably benign |
|
|
R5337:Gpr155
|
UTSW |
2 |
73,178,592 (GRCm39) |
missense |
probably benign |
0.32 |
|
R5830:Gpr155
|
UTSW |
2 |
73,200,433 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R5887:Gpr155
|
UTSW |
2 |
73,174,062 (GRCm39) |
nonsense |
probably null |
|
|
R5929:Gpr155
|
UTSW |
2 |
73,204,011 (GRCm39) |
nonsense |
probably null |
|
|
R6293:Gpr155
|
UTSW |
2 |
73,204,341 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
R6553:Gpr155
|
UTSW |
2 |
73,179,989 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7003:Gpr155
|
UTSW |
2 |
73,173,961 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7353:Gpr155
|
UTSW |
2 |
73,197,835 (GRCm39) |
nonsense |
probably null |
|
|
R7506:Gpr155
|
UTSW |
2 |
73,198,683 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R7631:Gpr155
|
UTSW |
2 |
73,213,291 (GRCm39) |
intron |
probably benign |
|
|
R7753:Gpr155
|
UTSW |
2 |
73,212,550 (GRCm39) |
missense |
probably benign |
0.27 |
|
R7810:Gpr155
|
UTSW |
2 |
73,212,296 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7813:Gpr155
|
UTSW |
2 |
73,212,329 (GRCm39) |
nonsense |
probably null |
|
|
R7815:Gpr155
|
UTSW |
2 |
73,192,904 (GRCm39) |
missense |
probably benign |
|
|
R7873:Gpr155
|
UTSW |
2 |
73,173,934 (GRCm39) |
missense |
possibly damaging |
0.51 |
|
R8506:Gpr155
|
UTSW |
2 |
73,173,806 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8680:Gpr155
|
UTSW |
2 |
73,174,039 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8856:Gpr155
|
UTSW |
2 |
73,203,993 (GRCm39) |
missense |
probably benign |
0.27 |
|
R8872:Gpr155
|
UTSW |
2 |
73,197,936 (GRCm39) |
missense |
probably benign |
0.02 |
|
R9116:Gpr155
|
UTSW |
2 |
73,204,109 (GRCm39) |
missense |
possibly damaging |
0.78 |
|
R9683:Gpr155
|
UTSW |
2 |
73,192,780 (GRCm39) |
missense |
probably benign |
|
|
| Predicted Primers |
PCR Primer
(F):5'- AGAAGTCTGTGCCTGTCAGC -3'
(R):5'- TGTCTACCCTGGATGCTGAATTTC -3'
Sequencing Primer
(F):5'- GCCTGTCAGCTCTTGTTAAAATG -3'
(R):5'- CTCGCTCACTAAGCATCT -3'
|
| Posted On |
2018-06-22 |
Incidental Mutation