Incidental Mutation 'R6585:Gm14851'
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ID524344
Institutional Source Beutler Lab
Gene Symbol Gm14851
Ensembl Gene ENSMUSG00000061958
Gene Namepredicted gene 14851
Synonyms
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.049) question?
Stock #R6585 (G1)
Quality Score225.009
Status Validated
Chromosome8
Chromosomal Location21095074-21096050 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 21095232 bp
ZygosityHeterozygous
Amino Acid Change Cysteine to Arginine at position 65 (C65R)
Ref Sequence ENSEMBL: ENSMUSP00000076665 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000077452]
Predicted Effect possibly damaging
Transcript: ENSMUST00000077452
AA Change: C65R

PolyPhen 2 Score 0.734 (Sensitivity: 0.85; Specificity: 0.92)
SMART Domains Protein: ENSMUSP00000076665
Gene: ENSMUSG00000061958
AA Change: C65R

DomainStartEndE-ValueType
Pfam:Defensin_propep 1 51 5.1e-25 PFAM
low complexity region 67 93 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000130905
Predicted Effect noncoding transcript
Transcript: ENSMUST00000177901
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.1%
  • 20x: 94.4%
Validation Efficiency 97% (30/31)
Allele List at MGI
Other mutations in this stock
Total: 30 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930522L14Rik C T 5: 109,737,668 C108Y probably damaging Het
AA792892 C T 5: 94,381,556 P62S probably benign Het
Adam1b G T 5: 121,501,187 D598E probably benign Het
Agr2 G A 12: 35,995,626 R37Q probably benign Het
Ascc3 A G 10: 50,842,177 K1989E probably benign Het
Chd1l A G 3: 97,597,772 F160L probably damaging Het
Ciita T A 16: 10,511,745 V628E probably benign Het
Dis3l2 T A 1: 86,745,494 I69N probably damaging Het
Dnase1l1 C T X: 74,277,038 probably null Homo
Elp2 T C 18: 24,625,549 L503S probably damaging Het
Fcgbp A T 7: 28,113,979 Q2313L possibly damaging Het
Gpr155 A T 2: 73,349,645 I157N probably damaging Het
Hist2h2bb A T 3: 96,270,097 T116S probably benign Het
Kcnj1 T C 9: 32,397,261 V307A probably benign Het
Lama3 G A 18: 12,419,257 probably null Het
Lrp6 A T 6: 134,507,558 Y367* probably null Het
Ms4a14 T A 19: 11,303,645 Q516H unknown Het
Nprl3 C T 11: 32,234,812 R399Q probably benign Het
Olfr273 A T 4: 52,856,192 M107K possibly damaging Het
Olfr290 A T 7: 84,916,462 I228F probably damaging Het
Park7 G T 4: 150,905,264 Q80K probably benign Het
Pramef20 C A 4: 144,377,030 L175F possibly damaging Het
Ptgs2 T C 1: 150,103,987 V281A possibly damaging Het
Rprd1a T C 18: 24,506,663 probably null Het
Speer4f2 A G 5: 17,374,422 E73G probably damaging Het
Spta1 T C 1: 174,178,685 W138R probably damaging Het
U2surp T C 9: 95,472,071 E838G probably damaging Het
Urb2 G T 8: 124,031,125 E1190D probably damaging Het
Usp19 G T 9: 108,499,727 L1165F probably damaging Het
Zfp27 G A 7: 29,896,393 T49I possibly damaging Het
Other mutations in Gm14851
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02051:Gm14851 APN 8 21095259 splice site probably benign
IGL02212:Gm14851 APN 8 21095260 splice site probably benign
R1495:Gm14851 UTSW 8 21095201 missense probably benign 0.33
R6045:Gm14851 UTSW 8 21095232 missense possibly damaging 0.53
Predicted Primers PCR Primer
(F):5'- GCAATTGAGCTAAGCACAATGG -3'
(R):5'- AATGTCTCGTGTCCATGGTG -3'

Sequencing Primer
(F):5'- CACAATGGGATCGTGTGGACC -3'
(R):5'- GCTGACTGTGATGTCACCAGTAC -3'
Posted On2018-06-22