Mutagenetix > Incidental Mutation

Incidental Mutation 'R6648:Cacna1g'

524370

Institutional Source

Beutler Lab

Gene Symbol

Cacna1g

Ensembl Gene

ENSMUSG00000020866

Gene Name

calcium channel, voltage-dependent, T type, alpha 1G subunit

Synonyms

a1G, Cav3.1d

MMRRC Submission

044769-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.450) question?

Stock #

R6648 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

94299217-94365024 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 94323395 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 1270 (C1270S)

Ref Sequence

ENSEMBL: ENSMUSP00000103422 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000021234] [ENSMUST00000100561] [ENSMUST00000103166] [ENSMUST00000107785] [ENSMUST00000107786] [ENSMUST00000107788] [ENSMUST00000107789] [ENSMUST00000107790] [ENSMUST00000107791] [ENSMUST00000107792] [ENSMUST00000107793]

AlphaFold

Q5SUF7

Predicted Effect

probably damaging
Transcript: ENSMUST00000021234
AA Change: C1247S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000021234
Gene: ENSMUSG00000020866
AA Change: C1247S

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	9.2e-66	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	7.9e-46	PFAM
low complexity region	1040	1051	N/A	INTRINSIC
low complexity region	1063	1090	N/A	INTRINSIC
low complexity region	1111	1127	N/A	INTRINSIC
low complexity region	1193	1204	N/A	INTRINSIC
Pfam:Ion_trans	1290	1514	3e-55	PFAM
coiled coil region	1519	1559	N/A	INTRINSIC
low complexity region	1562	1573	N/A	INTRINSIC
Pfam:Ion_trans	1630	1835	3e-49	PFAM
Pfam:PKD_channel	1688	1842	7.8e-11	PFAM
low complexity region	2180	2211	N/A	INTRINSIC
low complexity region	2230	2246	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000100561
AA Change: C1270S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000098127
Gene: ENSMUSG00000020866
AA Change: C1270S

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.9e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1063	1074	N/A	INTRINSIC
low complexity region	1086	1113	N/A	INTRINSIC
low complexity region	1134	1150	N/A	INTRINSIC
low complexity region	1216	1227	N/A	INTRINSIC
Pfam:Ion_trans	1313	1537	6.1e-55	PFAM
coiled coil region	1542	1582	N/A	INTRINSIC
low complexity region	1585	1596	N/A	INTRINSIC
Pfam:Ion_trans	1653	1858	6.2e-49	PFAM
Pfam:PKD_channel	1711	1865	1.4e-10	PFAM
low complexity region	2203	2234	N/A	INTRINSIC
low complexity region	2253	2269	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000103166
AA Change: C1270S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000099455
Gene: ENSMUSG00000020866
AA Change: C1270S

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.9e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1063	1074	N/A	INTRINSIC
low complexity region	1086	1113	N/A	INTRINSIC
low complexity region	1134	1150	N/A	INTRINSIC
low complexity region	1216	1227	N/A	INTRINSIC
Pfam:Ion_trans	1313	1537	6.1e-55	PFAM
SCOP:d1g2qa_	1545	1589	6e-3	SMART
Pfam:Ion_trans	1646	1851	6.2e-49	PFAM
Pfam:PKD_channel	1704	1858	1.4e-10	PFAM
low complexity region	2196	2227	N/A	INTRINSIC
low complexity region	2246	2262	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000107785
AA Change: C1247S

PolyPhen 2 Score 0.365 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000103414
Gene: ENSMUSG00000020866
AA Change: C1247S

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.8e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.5e-45	PFAM
low complexity region	1040	1051	N/A	INTRINSIC
low complexity region	1063	1090	N/A	INTRINSIC
low complexity region	1111	1127	N/A	INTRINSIC
low complexity region	1193	1204	N/A	INTRINSIC
Pfam:Ion_trans	1290	1514	5.7e-55	PFAM
coiled coil region	1519	1558	N/A	INTRINSIC
Pfam:Ion_trans	1612	1817	5.8e-49	PFAM
Pfam:PKD_channel	1670	1824	1.4e-10	PFAM
low complexity region	2027	2042	N/A	INTRINSIC
low complexity region	2084	2115	N/A	INTRINSIC
low complexity region	2134	2150	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000107786
AA Change: C1230S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000103415
Gene: ENSMUSG00000020866
AA Change: C1230S

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	383	4e-60	PFAM
low complexity region	466	490	N/A	INTRINSIC
low complexity region	518	530	N/A	INTRINSIC
Pfam:Ion_trans	760	946	1.6e-45	PFAM
low complexity region	1023	1034	N/A	INTRINSIC
low complexity region	1046	1073	N/A	INTRINSIC
low complexity region	1094	1110	N/A	INTRINSIC
low complexity region	1176	1187	N/A	INTRINSIC
Pfam:Ion_trans	1273	1497	5.9e-55	PFAM
SCOP:d1g2qa_	1505	1549	6e-3	SMART
Pfam:Ion_trans	1606	1811	6e-49	PFAM
Pfam:PKD_channel	1664	1818	1.4e-10	PFAM
low complexity region	2156	2187	N/A	INTRINSIC
low complexity region	2206	2222	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000107788
AA Change: C1270S

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000103417
Gene: ENSMUSG00000020866
AA Change: C1270S

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.9e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1063	1074	N/A	INTRINSIC
low complexity region	1086	1113	N/A	INTRINSIC
low complexity region	1134	1150	N/A	INTRINSIC
low complexity region	1216	1227	N/A	INTRINSIC
Pfam:Ion_trans	1313	1537	6e-55	PFAM
coiled coil region	1542	1574	N/A	INTRINSIC
Pfam:Ion_trans	1628	1833	6.1e-49	PFAM
Pfam:PKD_channel	1686	1840	1.4e-10	PFAM
low complexity region	2178	2209	N/A	INTRINSIC
low complexity region	2228	2244	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000107789
AA Change: C1270S

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000103418
Gene: ENSMUSG00000020866
AA Change: C1270S

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	80	406	1.6e-76	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	742	972	3.4e-56	PFAM
low complexity region	1063	1074	N/A	INTRINSIC
low complexity region	1086	1113	N/A	INTRINSIC
low complexity region	1134	1150	N/A	INTRINSIC
low complexity region	1216	1227	N/A	INTRINSIC
Pfam:Ion_trans	1276	1549	1.5e-61	PFAM
low complexity region	1578	1589	N/A	INTRINSIC
Pfam:Ion_trans	1611	1863	2.1e-56	PFAM
Pfam:PKD_channel	1703	1858	3.4e-9	PFAM
low complexity region	2289	2320	N/A	INTRINSIC
low complexity region	2339	2355	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000107790
AA Change: C1247S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000103419
Gene: ENSMUSG00000020866
AA Change: C1247S

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.9e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1040	1051	N/A	INTRINSIC
low complexity region	1063	1090	N/A	INTRINSIC
low complexity region	1111	1127	N/A	INTRINSIC
low complexity region	1193	1204	N/A	INTRINSIC
Pfam:Ion_trans	1290	1514	6e-55	PFAM
SCOP:d1g2qa_	1522	1566	6e-3	SMART
Pfam:Ion_trans	1623	1828	6.1e-49	PFAM
Pfam:PKD_channel	1681	1835	1.4e-10	PFAM
low complexity region	2173	2204	N/A	INTRINSIC
low complexity region	2223	2239	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000107791
AA Change: C1247S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000103420
Gene: ENSMUSG00000020866
AA Change: C1247S

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.8e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1040	1051	N/A	INTRINSIC
low complexity region	1063	1090	N/A	INTRINSIC
low complexity region	1111	1127	N/A	INTRINSIC
low complexity region	1193	1204	N/A	INTRINSIC
Pfam:Ion_trans	1290	1514	6e-55	PFAM
coiled coil region	1519	1558	N/A	INTRINSIC
Pfam:Ion_trans	1612	1817	6e-49	PFAM
Pfam:PKD_channel	1670	1824	1.4e-10	PFAM
low complexity region	2162	2193	N/A	INTRINSIC
low complexity region	2212	2228	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000107792
AA Change: C1247S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000103421
Gene: ENSMUSG00000020866
AA Change: C1247S

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.8e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1040	1051	N/A	INTRINSIC
low complexity region	1063	1090	N/A	INTRINSIC
low complexity region	1111	1127	N/A	INTRINSIC
low complexity region	1193	1204	N/A	INTRINSIC
Pfam:Ion_trans	1290	1514	5.9e-55	PFAM
coiled coil region	1519	1551	N/A	INTRINSIC
Pfam:Ion_trans	1605	1810	6e-49	PFAM
Pfam:PKD_channel	1663	1817	1.4e-10	PFAM
low complexity region	2155	2186	N/A	INTRINSIC
low complexity region	2205	2221	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000107793
AA Change: C1270S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000103422
Gene: ENSMUSG00000020866
AA Change: C1270S

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.9e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1063	1074	N/A	INTRINSIC
low complexity region	1086	1113	N/A	INTRINSIC
low complexity region	1134	1150	N/A	INTRINSIC
low complexity region	1216	1227	N/A	INTRINSIC
Pfam:Ion_trans	1313	1537	6e-55	PFAM
coiled coil region	1542	1581	N/A	INTRINSIC
Pfam:Ion_trans	1635	1840	6.1e-49	PFAM
Pfam:PKD_channel	1693	1847	1.4e-10	PFAM
low complexity region	2185	2216	N/A	INTRINSIC
low complexity region	2235	2251	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152811

Meta Mutation Damage Score

0.9485

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.6%
20x: 92.4%

Validation Efficiency

98% (42/43)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Voltage-sensitive calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division, and cell death. This gene encodes a T-type, low-voltage activated calcium channel. The T-type channels generate currents that are both transient, owing to fast inactivation, and tiny, owing to small conductance. T-type channels are thought to be involved in pacemaker activity, low-threshold calcium spikes, neuronal oscillations and resonance, and rebound burst firing. Many alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Sep 2011]
PHENOTYPE: Homozygote null mice display disrupted sleeping patterns, altered amounts of activity, abnormal action potentials in the brain, prolonged electrical conductance in the heart, and resistance to diet-induced obesity. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aars2	T	C	17: 45,827,490 (GRCm39)	Y511H	probably damaging	Het
Arhgef38	A	T	3: 132,838,236 (GRCm39)	I600N	probably damaging	Het
Ccar2	T	G	14: 70,376,674 (GRCm39)	D890A	probably benign	Het
Cep76	A	T	18: 67,752,804 (GRCm39)	V548D	probably benign	Het
Chat	T	G	14: 32,176,651 (GRCm39)	Q41P	probably benign	Het
Ddx24	T	A	12: 103,374,634 (GRCm39)	N850I	probably benign	Het
Dst	A	G	1: 34,301,122 (GRCm39)	I5879V	possibly damaging	Het
Ece1	A	G	4: 137,648,470 (GRCm39)	T92A	probably benign	Het
Ecrg4	T	A	1: 43,777,851 (GRCm39)	F87I	probably damaging	Het
Fpgs	G	T	2: 32,574,799 (GRCm39)	C439*	probably null	Het
H2-Q4	A	T	17: 35,601,981 (GRCm39)	E281V	probably damaging	Het
Hydin	A	G	8: 111,252,299 (GRCm39)		probably null	Het
Iqgap2	T	C	13: 95,818,719 (GRCm39)	E679G	probably benign	Het
Kif12	A	T	4: 63,089,554 (GRCm39)		probably null	Het
Kif21b	T	C	1: 136,080,135 (GRCm39)	V557A	probably benign	Het
Mki67	C	G	7: 135,299,169 (GRCm39)	S1955T	probably damaging	Het
Mybpc1	G	A	10: 88,358,861 (GRCm39)	T1110M	probably damaging	Het
Myh9	T	C	15: 77,650,972 (GRCm39)	H1625R	probably benign	Het
Nbeal2	G	A	9: 110,466,710 (GRCm39)	T704M	probably damaging	Het
Or1ad6	G	A	11: 50,860,000 (GRCm39)	D52N	probably damaging	Het
Or5a21	A	T	19: 12,311,130 (GRCm39)	M30K	probably benign	Het
Osbpl9	C	A	4: 108,948,876 (GRCm39)	Q91H	possibly damaging	Het
Pcsk5	A	T	19: 17,553,185 (GRCm39)	L665Q	probably damaging	Het
Pgm5	T	C	19: 24,838,996 (GRCm39)	D53G	probably benign	Het
Plcd4	A	G	1: 74,591,172 (GRCm39)	D189G	probably benign	Het
Plxnb1	A	T	9: 108,933,398 (GRCm39)	Q676L	probably benign	Het
Prss33	A	G	17: 24,053,676 (GRCm39)	S134P	probably benign	Het
Rln1	G	T	19: 29,309,521 (GRCm39)	T86K	probably benign	Het
Rnf213	G	A	11: 119,370,746 (GRCm39)	A4785T	possibly damaging	Het
Rsf1	GGCG	GGCGACGGCCGCG	7: 97,229,113 (GRCm39)		probably benign	Het
Sh3tc2	T	C	18: 62,148,111 (GRCm39)	C1274R	probably benign	Het
Slc35a5	A	T	16: 44,964,280 (GRCm39)	F318I	probably damaging	Het
Slc41a3	T	C	6: 90,596,490 (GRCm39)	F20L	probably damaging	Het
Socs4	T	A	14: 47,527,633 (GRCm39)	N189K	probably benign	Het
Tiam2	A	T	17: 3,557,148 (GRCm39)	E1196V	probably damaging	Het
Tmc3	T	A	7: 83,246,751 (GRCm39)	V164D	probably damaging	Het
Trim46	T	C	3: 89,142,549 (GRCm39)	E623G	possibly damaging	Het
Ubr4	C	A	4: 139,180,030 (GRCm39)	C3403*	probably null	Het
Vmn1r194	A	T	13: 22,428,694 (GRCm39)	T104S	probably benign	Het
Vmn2r112	A	T	17: 22,837,467 (GRCm39)	I643F	probably damaging	Het
Zfp1002	T	C	2: 150,097,097 (GRCm39)	I111V	probably benign	Het
Zmynd11	T	G	13: 9,763,057 (GRCm39)	S95R	probably benign	Het
Zswim4	C	A	8: 84,957,543 (GRCm39)	R175L	probably benign	Het

Other mutations in Cacna1g

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01300:Cacna1g	APN	11	94,324,738 (GRCm39)	missense	probably benign	0.16
IGL01382:Cacna1g	APN	11	94,356,684 (GRCm39)	missense	probably damaging	1.00
IGL01694:Cacna1g	APN	11	94,319,938 (GRCm39)	missense	probably damaging	1.00
IGL01866:Cacna1g	APN	11	94,347,937 (GRCm39)	missense	probably damaging	0.99
IGL02341:Cacna1g	APN	11	94,352,978 (GRCm39)	missense	probably damaging	1.00
IGL02506:Cacna1g	APN	11	94,319,955 (GRCm39)	missense	probably damaging	0.99
IGL02706:Cacna1g	APN	11	94,347,818 (GRCm39)	missense	probably damaging	1.00
IGL02879:Cacna1g	APN	11	94,300,431 (GRCm39)	missense	probably benign	0.12
IGL03366:Cacna1g	APN	11	94,347,977 (GRCm39)	missense	probably damaging	1.00
ANU23:Cacna1g	UTSW	11	94,357,054 (GRCm39)	critical splice acceptor site	probably benign
IGL03134:Cacna1g	UTSW	11	94,350,651 (GRCm39)	missense	probably damaging	1.00
R0092:Cacna1g	UTSW	11	94,348,090 (GRCm39)	missense	probably damaging	1.00
R0123:Cacna1g	UTSW	11	94,300,302 (GRCm39)	missense	probably damaging	1.00
R0178:Cacna1g	UTSW	11	94,354,309 (GRCm39)	missense	probably damaging	1.00
R0375:Cacna1g	UTSW	11	94,301,880 (GRCm39)	missense	possibly damaging	0.79
R0389:Cacna1g	UTSW	11	94,350,523 (GRCm39)	missense	probably damaging	1.00
R0433:Cacna1g	UTSW	11	94,350,033 (GRCm39)	missense	probably benign	0.16
R0458:Cacna1g	UTSW	11	94,300,266 (GRCm39)	missense	probably damaging	0.96
R0498:Cacna1g	UTSW	11	94,350,685 (GRCm39)	missense	probably damaging	1.00
R0629:Cacna1g	UTSW	11	94,300,369 (GRCm39)	missense	possibly damaging	0.93
R0800:Cacna1g	UTSW	11	94,317,265 (GRCm39)	missense	probably damaging	1.00
R1341:Cacna1g	UTSW	11	94,324,582 (GRCm39)	missense	probably damaging	0.99
R1457:Cacna1g	UTSW	11	94,350,381 (GRCm39)	missense	possibly damaging	0.76
R1523:Cacna1g	UTSW	11	94,333,555 (GRCm39)	critical splice donor site	probably null
R1532:Cacna1g	UTSW	11	94,334,157 (GRCm39)	missense	probably damaging	1.00
R1540:Cacna1g	UTSW	11	94,347,865 (GRCm39)	missense	probably damaging	1.00
R1652:Cacna1g	UTSW	11	94,318,230 (GRCm39)	missense	probably damaging	1.00
R1688:Cacna1g	UTSW	11	94,316,779 (GRCm39)	missense	possibly damaging	0.70
R1750:Cacna1g	UTSW	11	94,334,118 (GRCm39)	missense	probably damaging	1.00
R1751:Cacna1g	UTSW	11	94,350,628 (GRCm39)	missense	probably benign	0.35
R1767:Cacna1g	UTSW	11	94,350,628 (GRCm39)	missense	probably benign	0.35
R1847:Cacna1g	UTSW	11	94,357,007 (GRCm39)	missense	probably damaging	1.00
R1924:Cacna1g	UTSW	11	94,334,880 (GRCm39)	missense	possibly damaging	0.93
R1973:Cacna1g	UTSW	11	94,350,603 (GRCm39)	missense	possibly damaging	0.86
R2050:Cacna1g	UTSW	11	94,300,300 (GRCm39)	missense	probably damaging	1.00
R2261:Cacna1g	UTSW	11	94,347,961 (GRCm39)	missense	probably benign	0.42
R2273:Cacna1g	UTSW	11	94,306,762 (GRCm39)	missense	probably damaging	0.98
R2274:Cacna1g	UTSW	11	94,306,762 (GRCm39)	missense	probably damaging	0.98
R2275:Cacna1g	UTSW	11	94,306,762 (GRCm39)	missense	probably damaging	0.98
R2376:Cacna1g	UTSW	11	94,356,734 (GRCm39)	missense	probably damaging	0.99
R3686:Cacna1g	UTSW	11	94,349,916 (GRCm39)	splice site	probably null
R3809:Cacna1g	UTSW	11	94,306,922 (GRCm39)	missense	probably damaging	1.00
R3875:Cacna1g	UTSW	11	94,328,749 (GRCm39)	missense	probably damaging	0.98
R4133:Cacna1g	UTSW	11	94,323,370 (GRCm39)	missense	probably damaging	1.00
R4642:Cacna1g	UTSW	11	94,308,920 (GRCm39)	missense	probably damaging	1.00
R4732:Cacna1g	UTSW	11	94,334,041 (GRCm39)	missense	probably damaging	1.00
R4733:Cacna1g	UTSW	11	94,334,041 (GRCm39)	missense	probably damaging	1.00
R4773:Cacna1g	UTSW	11	94,302,298 (GRCm39)	missense	possibly damaging	0.78
R4798:Cacna1g	UTSW	11	94,324,673 (GRCm39)	missense	probably damaging	1.00
R4839:Cacna1g	UTSW	11	94,350,433 (GRCm39)	missense	probably benign	0.16
R4900:Cacna1g	UTSW	11	94,350,177 (GRCm39)	missense	possibly damaging	0.78
R4927:Cacna1g	UTSW	11	94,319,973 (GRCm39)	missense	probably damaging	1.00
R4930:Cacna1g	UTSW	11	94,334,899 (GRCm39)	missense	probably damaging	1.00
R5050:Cacna1g	UTSW	11	94,350,541 (GRCm39)	missense	probably damaging	1.00
R5117:Cacna1g	UTSW	11	94,323,329 (GRCm39)	missense	probably damaging	1.00
R5186:Cacna1g	UTSW	11	94,333,674 (GRCm39)	missense	probably damaging	1.00
R5364:Cacna1g	UTSW	11	94,307,684 (GRCm39)	missense	probably benign	0.29
R5512:Cacna1g	UTSW	11	94,334,968 (GRCm39)	missense	probably damaging	1.00
R5564:Cacna1g	UTSW	11	94,321,312 (GRCm39)	missense	probably damaging	1.00
R5603:Cacna1g	UTSW	11	94,330,578 (GRCm39)	missense	possibly damaging	0.76
R5682:Cacna1g	UTSW	11	94,349,940 (GRCm39)	missense	probably damaging	1.00
R5818:Cacna1g	UTSW	11	94,308,946 (GRCm39)	missense	probably damaging	1.00
R5828:Cacna1g	UTSW	11	94,347,980 (GRCm39)	missense	probably damaging	1.00
R5882:Cacna1g	UTSW	11	94,350,645 (GRCm39)	missense	probably damaging	1.00
R5884:Cacna1g	UTSW	11	94,328,693 (GRCm39)	missense	probably damaging	1.00
R6075:Cacna1g	UTSW	11	94,307,491 (GRCm39)	missense	probably damaging	1.00
R6112:Cacna1g	UTSW	11	94,300,072 (GRCm39)	missense	probably damaging	0.98
R6122:Cacna1g	UTSW	11	94,320,997 (GRCm39)	missense	probably benign	0.11
R6145:Cacna1g	UTSW	11	94,353,087 (GRCm39)	missense	probably damaging	1.00
R6362:Cacna1g	UTSW	11	94,330,533 (GRCm39)	critical splice donor site	probably null
R6415:Cacna1g	UTSW	11	94,354,243 (GRCm39)	missense	probably damaging	1.00
R6468:Cacna1g	UTSW	11	94,330,548 (GRCm39)	missense	probably damaging	1.00
R6746:Cacna1g	UTSW	11	94,300,253 (GRCm39)	nonsense	probably null
R6764:Cacna1g	UTSW	11	94,304,014 (GRCm39)	missense	possibly damaging	0.95
R6782:Cacna1g	UTSW	11	94,350,376 (GRCm39)	missense	probably damaging	1.00
R6888:Cacna1g	UTSW	11	94,350,033 (GRCm39)	missense	probably benign	0.16
R7148:Cacna1g	UTSW	11	94,356,756 (GRCm39)	missense	probably benign	0.32
R7181:Cacna1g	UTSW	11	94,306,691 (GRCm39)	missense	probably benign	0.21
R7183:Cacna1g	UTSW	11	94,330,563 (GRCm39)	missense	probably benign	0.04
R7193:Cacna1g	UTSW	11	94,300,057 (GRCm39)	missense	possibly damaging	0.60
R7237:Cacna1g	UTSW	11	94,328,705 (GRCm39)	missense	probably benign	0.21
R7254:Cacna1g	UTSW	11	94,323,393 (GRCm39)	nonsense	probably null
R7312:Cacna1g	UTSW	11	94,323,383 (GRCm39)	missense	probably damaging	1.00
R7451:Cacna1g	UTSW	11	94,319,901 (GRCm39)	missense	probably damaging	1.00
R7470:Cacna1g	UTSW	11	94,352,765 (GRCm39)	missense	possibly damaging	0.76
R7534:Cacna1g	UTSW	11	94,301,904 (GRCm39)	missense	probably benign	0.00
R7585:Cacna1g	UTSW	11	94,364,368 (GRCm39)	missense	probably benign	0.39
R7706:Cacna1g	UTSW	11	94,305,867 (GRCm39)	missense	probably benign	0.06
R7812:Cacna1g	UTSW	11	94,334,880 (GRCm39)	missense	probably benign	0.03
R7918:Cacna1g	UTSW	11	94,334,856 (GRCm39)	missense	probably benign	0.03
R7947:Cacna1g	UTSW	11	94,348,001 (GRCm39)	missense	probably benign	0.01
R8013:Cacna1g	UTSW	11	94,347,796 (GRCm39)	missense	probably damaging	0.99
R8016:Cacna1g	UTSW	11	94,334,007 (GRCm39)	missense	probably benign	0.36
R8029:Cacna1g	UTSW	11	94,300,564 (GRCm39)	missense	probably benign	0.01
R8098:Cacna1g	UTSW	11	94,307,338 (GRCm39)	missense	probably benign	0.20
R8264:Cacna1g	UTSW	11	94,364,392 (GRCm39)	missense	probably benign	0.21
R8478:Cacna1g	UTSW	11	94,317,278 (GRCm39)	missense	probably damaging	1.00
R8679:Cacna1g	UTSW	11	94,319,962 (GRCm39)	missense	probably damaging	1.00
R8697:Cacna1g	UTSW	11	94,307,524 (GRCm39)	missense	probably benign	0.09
R8772:Cacna1g	UTSW	11	94,356,713 (GRCm39)	missense	probably benign	0.03
R9011:Cacna1g	UTSW	11	94,306,663 (GRCm39)	missense	probably benign	0.21
R9085:Cacna1g	UTSW	11	94,334,046 (GRCm39)	missense	probably benign	0.01
R9155:Cacna1g	UTSW	11	94,350,423 (GRCm39)	missense
R9243:Cacna1g	UTSW	11	94,347,893 (GRCm39)	missense	possibly damaging	0.84
R9288:Cacna1g	UTSW	11	94,308,897 (GRCm39)	nonsense	probably null
R9408:Cacna1g	UTSW	11	94,321,050 (GRCm39)	missense	probably damaging	1.00
R9496:Cacna1g	UTSW	11	94,356,711 (GRCm39)	missense	probably benign	0.03
R9607:Cacna1g	UTSW	11	94,356,714 (GRCm39)	missense	probably benign	0.03
R9720:Cacna1g	UTSW	11	94,302,297 (GRCm39)	missense	probably benign	0.01
X0001:Cacna1g	UTSW	11	94,300,471 (GRCm39)	missense	possibly damaging	0.90
X0019:Cacna1g	UTSW	11	94,350,079 (GRCm39)	missense	probably damaging	0.97
X0065:Cacna1g	UTSW	11	94,353,251 (GRCm39)	missense	probably damaging	1.00
Z1176:Cacna1g	UTSW	11	94,328,937 (GRCm39)	missense	probably benign	0.31
Z1177:Cacna1g	UTSW	11	94,364,416 (GRCm39)	missense	probably benign	0.21
Z1177:Cacna1g	UTSW	11	94,350,422 (GRCm39)	missense	probably benign	0.04

Predicted Primers

PCR Primer
(F):5'- TGTGTGACCTTGCACAGACTG -3'
(R):5'- AGAGCGAGATTCCTGGTCTG -3'

Sequencing Primer
(F):5'- TGGAGACAGTTTGAGCCTTCTCAC -3'
(R):5'- GCGAGATTCCTGGTCTGCCTATATC -3'

Posted On

2018-06-22