Mutagenetix > Incidental Mutation

Incidental Mutation 'R6602:Ccdc162'

525367

Institutional Source

Beutler Lab

Gene Symbol

Ccdc162

Ensembl Gene

ENSMUSG00000075225

Gene Name

coiled-coil domain containing 162

Synonyms

5033413D22Rik

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.063) question?

Stock #

R6602 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

41538846-41716634 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 41615980 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Lysine at position 1079 (T1079K)

Ref Sequence

ENSEMBL: ENSMUSP00000140774 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000019955] [ENSMUST00000099932] [ENSMUST00000189488] [ENSMUST00000219054]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000019955

SMART Domains

Protein: ENSMUSP00000019955
Gene: ENSMUSG00000075225

Domain	Start	End	E-Value	Type
coiled coil region	1	37	N/A	INTRINSIC
low complexity region	116	138	N/A	INTRINSIC
coiled coil region	177	217	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000099932

SMART Domains

Protein: ENSMUSP00000097516
Gene: ENSMUSG00000075225

Domain	Start	End	E-Value	Type
coiled coil region	327	366	N/A	INTRINSIC
low complexity region	490	512	N/A	INTRINSIC
coiled coil region	551	607	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000187853

Predicted Effect

probably benign
Transcript: ENSMUST00000189488
AA Change: T1079K

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000140774
Gene: ENSMUSG00000075225
AA Change: T1079K

Domain	Start	End	E-Value	Type
low complexity region	328	347	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000219054

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.6%
20x: 92.6%

Validation Efficiency

100% (43/43)

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310022B05Rik	A	G	8: 124,639,254 (GRCm38)	L250P	probably damaging	Het
4921539E11Rik	T	C	4: 103,255,572 (GRCm38)	H12R	probably benign	Het
Abca4	A	C	3: 122,138,501 (GRCm38)	Q268P	probably benign	Het
Adgrf5	T	A	17: 43,450,304 (GRCm38)	N963K	probably benign	Het
Arl10	A	G	13: 54,578,937 (GRCm38)	D176G	probably damaging	Het
Btnl1	T	A	17: 34,385,748 (GRCm38)	M501K	probably damaging	Het
Cd163	T	A	6: 124,311,635 (GRCm38)	W342R	probably damaging	Het
Cd70	T	C	17: 57,149,562 (GRCm38)	S14G	probably benign	Het
Chil4	C	A	3: 106,210,590 (GRCm38)	K121N	probably benign	Het
Csf1r	A	G	18: 61,110,425 (GRCm38)	D171G	possibly damaging	Het
Cyp4a31	A	T	4: 115,569,707 (GRCm38)		probably null	Het
D3Ertd254e	G	T	3: 36,164,855 (GRCm38)	L341F	possibly damaging	Het
Dapk1	A	T	13: 60,749,204 (GRCm38)	I746F	probably benign	Het
Erbb4	A	G	1: 68,370,503 (GRCm38)	S192P	probably damaging	Het
Exoc8	C	A	8: 124,896,411 (GRCm38)	V406L	probably damaging	Het
Fam168b	C	A	1: 34,836,741 (GRCm38)	G21V	probably damaging	Het
Greb1	A	T	12: 16,709,440 (GRCm38)	V652E	probably benign	Het
Ift88	A	G	14: 57,507,259 (GRCm38)	S745G	probably benign	Het
Il18bp	T	C	7: 102,016,030 (GRCm38)		probably benign	Het
Il6st	A	G	13: 112,504,413 (GRCm38)	T908A	probably damaging	Het
Klk11	A	G	7: 43,774,774 (GRCm38)	S6G	probably benign	Het
Mastl	T	C	2: 23,132,677 (GRCm38)	Y678C	probably benign	Het
Msra	A	T	14: 64,123,339 (GRCm38)	H184Q	probably benign	Het
Muc16	A	C	9: 18,609,476 (GRCm38)		probably null	Het
Myo3a	T	G	2: 22,577,787 (GRCm38)	L351R	probably damaging	Het
Npy5r	GCTGTGAAACACTG	GCTG	8: 66,681,540 (GRCm38)		probably null	Het
Olfr463	T	C	11: 87,893,652 (GRCm38)	T91A	probably benign	Het
Olfr830	A	G	9: 18,875,849 (GRCm38)	D174G	possibly damaging	Het
Olfr867	C	T	9: 20,055,046 (GRCm38)	R139Q	probably benign	Het
Pcdhb18	A	T	18: 37,490,480 (GRCm38)	I288F	probably damaging	Het
Pitpna	T	G	11: 75,620,315 (GRCm38)	V238G	possibly damaging	Het
Ppfibp1	T	A	6: 146,978,221 (GRCm38)	V81E	possibly damaging	Het
Rab11fip2	T	A	19: 59,942,856 (GRCm38)	T49S	probably damaging	Het
Rsl24d1	T	A	9: 73,113,510 (GRCm38)	I3N	possibly damaging	Het
Rtn1	T	C	12: 72,219,318 (GRCm38)	N161S	probably damaging	Het
Shank1	A	G	7: 44,352,336 (GRCm38)	I1151V	probably benign	Het
Slc34a3	A	G	2: 25,229,209 (GRCm38)	S550P	probably damaging	Het
Slc4a1ap	A	G	5: 31,527,641 (GRCm38)	H207R	probably damaging	Het
Sphkap	T	A	1: 83,275,758 (GRCm38)	K1423N	possibly damaging	Het
Ttn	A	G	2: 76,881,753 (GRCm38)		probably benign	Het
Ubqln5	T	A	7: 104,129,489 (GRCm38)	S43C	probably benign	Het
Vps13d	G	A	4: 145,103,664 (GRCm38)		probably benign	Het
Wwp1	A	T	4: 19,641,816 (GRCm38)	V413D	probably damaging	Het

Other mutations in Ccdc162

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01013:Ccdc162	APN	10	41,581,339 (GRCm38)	missense	probably benign	0.01
IGL01366:Ccdc162	APN	10	41,580,306 (GRCm38)	missense	possibly damaging	0.49
IGL01924:Ccdc162	APN	10	41,569,887 (GRCm38)	missense	probably damaging	1.00
IGL02504:Ccdc162	APN	10	41,552,388 (GRCm38)	missense	probably damaging	1.00
IGL02678:Ccdc162	APN	10	41,561,155 (GRCm38)	missense	probably damaging	0.99
IGL02955:Ccdc162	APN	10	41,561,127 (GRCm38)	missense	probably damaging	1.00
beeswax	UTSW	10	41,561,226 (GRCm38)	missense	possibly damaging	0.57
honeycomb	UTSW	10	41,644,641 (GRCm38)	missense	probably benign	0.35
FR4304:Ccdc162	UTSW	10	41,556,121 (GRCm38)	missense	possibly damaging	0.49
R0432:Ccdc162	UTSW	10	41,541,860 (GRCm38)	missense	probably benign	0.01
R0585:Ccdc162	UTSW	10	41,586,379 (GRCm38)	missense	probably benign	0.03
R0645:Ccdc162	UTSW	10	41,586,411 (GRCm38)	splice site	probably benign
R0731:Ccdc162	UTSW	10	41,579,143 (GRCm38)	missense	probably damaging	1.00
R1426:Ccdc162	UTSW	10	41,553,182 (GRCm38)	missense	possibly damaging	0.89
R1447:Ccdc162	UTSW	10	41,580,247 (GRCm38)	missense	probably damaging	1.00
R1712:Ccdc162	UTSW	10	41,539,431 (GRCm38)	missense	probably benign	0.35
R2138:Ccdc162	UTSW	10	41,581,297 (GRCm38)	missense	probably benign	0.15
R2351:Ccdc162	UTSW	10	41,555,972 (GRCm38)	critical splice donor site	probably null
R2394:Ccdc162	UTSW	10	41,569,898 (GRCm38)	missense	probably damaging	1.00
R2431:Ccdc162	UTSW	10	41,569,845 (GRCm38)	missense	probably benign
R2571:Ccdc162	UTSW	10	41,552,397 (GRCm38)	missense	probably damaging	1.00
R2873:Ccdc162	UTSW	10	41,655,099 (GRCm38)	missense	possibly damaging	0.68
R2926:Ccdc162	UTSW	10	41,561,207 (GRCm38)	start gained	probably benign
R2999:Ccdc162	UTSW	10	41,580,290 (GRCm38)	missense	probably benign	0.00
R3412:Ccdc162	UTSW	10	41,539,549 (GRCm38)	splice site	probably benign
R3712:Ccdc162	UTSW	10	41,587,379 (GRCm38)	missense	probably benign
R3736:Ccdc162	UTSW	10	41,589,568 (GRCm38)	splice site	probably null
R4112:Ccdc162	UTSW	10	41,656,328 (GRCm38)	missense	possibly damaging	0.77
R4557:Ccdc162	UTSW	10	41,587,388 (GRCm38)	missense	probably benign	0.01
R4580:Ccdc162	UTSW	10	41,561,140 (GRCm38)	missense	probably benign	0.02
R4685:Ccdc162	UTSW	10	41,681,686 (GRCm38)	missense	possibly damaging	0.89
R4837:Ccdc162	UTSW	10	41,673,867 (GRCm38)	missense	probably benign	0.00
R5155:Ccdc162	UTSW	10	41,579,151 (GRCm38)	missense	probably damaging	1.00
R5155:Ccdc162	UTSW	10	41,553,580 (GRCm38)	splice site	probably null
R5645:Ccdc162	UTSW	10	41,552,356 (GRCm38)	missense	probably benign	0.06
R5656:Ccdc162	UTSW	10	41,569,934 (GRCm38)	missense	probably benign	0.26
R5682:Ccdc162	UTSW	10	41,556,803 (GRCm38)	nonsense	probably null
R5808:Ccdc162	UTSW	10	41,655,504 (GRCm38)	missense	possibly damaging	0.62
R5909:Ccdc162	UTSW	10	41,561,115 (GRCm38)	missense	probably damaging	1.00
R6000:Ccdc162	UTSW	10	41,561,163 (GRCm38)	missense	possibly damaging	0.75
R6057:Ccdc162	UTSW	10	41,634,041 (GRCm38)	missense	possibly damaging	0.72
R6211:Ccdc162	UTSW	10	41,630,145 (GRCm38)	nonsense	probably null
R6264:Ccdc162	UTSW	10	41,694,468 (GRCm38)	missense	probably benign	0.31
R6329:Ccdc162	UTSW	10	41,663,151 (GRCm38)	missense	possibly damaging	0.76
R6349:Ccdc162	UTSW	10	41,694,400 (GRCm38)	missense	probably damaging	0.97
R6398:Ccdc162	UTSW	10	41,627,149 (GRCm38)	missense	probably damaging	1.00
R6453:Ccdc162	UTSW	10	41,550,825 (GRCm38)	missense	probably damaging	1.00
R6627:Ccdc162	UTSW	10	41,663,185 (GRCm38)	missense	probably damaging	1.00
R6722:Ccdc162	UTSW	10	41,644,641 (GRCm38)	missense	probably benign	0.35
R6750:Ccdc162	UTSW	10	41,561,226 (GRCm38)	missense	possibly damaging	0.57
R6968:Ccdc162	UTSW	10	41,673,844 (GRCm38)	missense	possibly damaging	0.55
R6970:Ccdc162	UTSW	10	41,615,958 (GRCm38)	missense	probably benign	0.03
R6989:Ccdc162	UTSW	10	41,581,353 (GRCm38)	missense	probably damaging	0.99
R7008:Ccdc162	UTSW	10	41,552,415 (GRCm38)	missense	probably damaging	1.00
R7135:Ccdc162	UTSW	10	41,673,859 (GRCm38)	missense	probably benign	0.00
R7139:Ccdc162	UTSW	10	41,666,721 (GRCm38)	missense	possibly damaging	0.49
R7224:Ccdc162	UTSW	10	41,561,191 (GRCm38)	missense	probably damaging	1.00
R7230:Ccdc162	UTSW	10	41,678,813 (GRCm38)	missense	probably damaging	1.00
R7256:Ccdc162	UTSW	10	41,556,001 (GRCm38)	missense	probably damaging	0.99
R7261:Ccdc162	UTSW	10	41,561,140 (GRCm38)	missense	probably benign	0.02
R7390:Ccdc162	UTSW	10	41,634,048 (GRCm38)	missense	probably benign
R7712:Ccdc162	UTSW	10	41,627,227 (GRCm38)	missense	possibly damaging	0.56
R7726:Ccdc162	UTSW	10	41,553,075 (GRCm38)	missense	probably benign	0.00
R7754:Ccdc162	UTSW	10	41,587,375 (GRCm38)	missense	probably damaging	1.00
R7764:Ccdc162	UTSW	10	41,690,113 (GRCm38)	missense	possibly damaging	0.95
R8053:Ccdc162	UTSW	10	41,644,581 (GRCm38)	missense	probably benign
R8088:Ccdc162	UTSW	10	41,623,414 (GRCm38)	missense	possibly damaging	0.68
R8094:Ccdc162	UTSW	10	41,612,868 (GRCm38)	missense	probably benign	0.02
R8097:Ccdc162	UTSW	10	41,634,119 (GRCm38)	missense	probably benign	0.03
R8321:Ccdc162	UTSW	10	41,634,033 (GRCm38)	missense	probably damaging	0.98
R8377:Ccdc162	UTSW	10	41,581,310 (GRCm38)	missense	probably benign	0.08
R8399:Ccdc162	UTSW	10	41,539,521 (GRCm38)	missense	probably damaging	1.00
R8669:Ccdc162	UTSW	10	41,552,356 (GRCm38)	missense	probably benign	0.06
R8772:Ccdc162	UTSW	10	41,630,037 (GRCm38)	missense	probably damaging	0.99
R8810:Ccdc162	UTSW	10	41,666,741 (GRCm38)	missense	probably benign	0.41
R8903:Ccdc162	UTSW	10	41,655,444 (GRCm38)	critical splice donor site	probably null
R8928:Ccdc162	UTSW	10	41,586,249 (GRCm38)	splice site	probably benign
R8950:Ccdc162	UTSW	10	41,598,511 (GRCm38)	missense	probably benign	0.00
R8960:Ccdc162	UTSW	10	41,553,182 (GRCm38)	missense	probably damaging	0.96
R8985:Ccdc162	UTSW	10	41,556,106 (GRCm38)	missense	probably damaging	1.00
R9071:Ccdc162	UTSW	10	41,581,178 (GRCm38)	nonsense	probably null
R9254:Ccdc162	UTSW	10	41,612,948 (GRCm38)	critical splice acceptor site	probably null
R9297:Ccdc162	UTSW	10	41,630,114 (GRCm38)	missense	probably benign
R9318:Ccdc162	UTSW	10	41,630,114 (GRCm38)	missense	probably benign
R9518:Ccdc162	UTSW	10	41,589,576 (GRCm38)	missense	probably damaging	1.00
R9525:Ccdc162	UTSW	10	41,683,226 (GRCm38)	missense	probably damaging	0.99
R9539:Ccdc162	UTSW	10	41,587,411 (GRCm38)	missense	possibly damaging	0.54
R9638:Ccdc162	UTSW	10	41,561,163 (GRCm38)	missense	probably benign	0.01
Z1176:Ccdc162	UTSW	10	41,654,997 (GRCm38)	missense	possibly damaging	0.84
Z1176:Ccdc162	UTSW	10	41,605,108 (GRCm38)	missense	possibly damaging	0.84
Z1176:Ccdc162	UTSW	10	41,553,131 (GRCm38)	missense	probably benign	0.00
Z1176:Ccdc162	UTSW	10	41,690,092 (GRCm38)	missense	probably benign	0.00
Z1177:Ccdc162	UTSW	10	41,683,195 (GRCm38)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AGCTGAAGGAGTGAGTGTTC -3'
(R):5'- CCCCTACTGTGTGCAAAGTG -3'

Sequencing Primer
(F):5'- CACACACACTCTGTTTTTGATTGAG -3'
(R):5'- CCCTACTGTGTGCAAAGTGTTGTC -3'

Posted On

2018-06-22