Mutagenetix > Incidental Mutation

Incidental Mutation 'R6605:Zfp772'

525584

Institutional Source

Beutler Lab

Gene Symbol

Zfp772

Ensembl Gene

ENSMUSG00000066838

Gene Name

zinc finger protein 772

Synonyms

MMRRC Submission

044728-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.065) question?

Stock #

R6605 (G1)

Quality Score

146.011

Status

Validated

Chromosome

Chromosomal Location

7205121-7212997 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 7208547 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 99 (E99G)

Ref Sequence

ENSEMBL: ENSMUSP00000074055 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074455]

AlphaFold

Q3UQL6

Predicted Effect

possibly damaging
Transcript: ENSMUST00000074455
AA Change: E99G

PolyPhen 2 Score 0.719 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000074055
Gene: ENSMUSG00000066838
AA Change: E99G

Domain	Start	End	E-Value	Type
KRAB	42	102	1.87e-14	SMART
ZnF_C2H2	193	215	1.36e-2	SMART
ZnF_C2H2	221	243	5.21e-4	SMART
ZnF_C2H2	249	271	1.38e-3	SMART
ZnF_C2H2	277	299	4.24e-4	SMART
ZnF_C2H2	305	327	5.21e-4	SMART
ZnF_C2H2	333	355	2.27e-4	SMART
ZnF_C2H2	361	383	1.18e-2	SMART
ZnF_C2H2	389	411	4.87e-4	SMART

Meta Mutation Damage Score

0.1163

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.9%
20x: 93.8%

Validation Efficiency

92% (35/38)

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc6	T	A	7: 45,630,481 (GRCm39)	I1260F	probably damaging	Het
Acat2	A	G	17: 13,162,774 (GRCm39)	V358A	probably benign	Het
Adgrv1	G	A	13: 81,636,081 (GRCm39)	A3476V	possibly damaging	Het
Akap13	A	G	7: 75,229,516 (GRCm39)	N150D	probably damaging	Het
Asb2	G	T	12: 103,311,943 (GRCm39)	Q60K	probably benign	Het
Asxl3	A	T	18: 22,650,134 (GRCm39)	R708*	probably null	Het
Cd79b	C	T	11: 106,203,539 (GRCm39)	G116D	probably damaging	Het
Copg2	A	G	6: 30,835,757 (GRCm39)	F218S	probably benign	Het
Defa5	A	C	8: 21,787,604 (GRCm39)	E50D	possibly damaging	Het
Efcab3	T	A	11: 104,890,107 (GRCm39)		probably null	Het
Eva1c	T	A	16: 90,663,236 (GRCm39)	V91D	probably damaging	Het
Fosb	T	C	7: 19,043,283 (GRCm39)	Y25C	probably damaging	Het
Gm5141	G	A	13: 62,922,201 (GRCm39)	H323Y	probably damaging	Het
Gpr19	T	C	6: 134,847,398 (GRCm39)	N58S	probably benign	Het
Gpr26	G	A	7: 131,585,893 (GRCm39)	A288T	possibly damaging	Het
Gucy2g	T	A	19: 55,229,460 (GRCm39)	Q70L	probably damaging	Het
Ifit2	C	T	19: 34,550,897 (GRCm39)	R146*	probably null	Het
Lgr5	A	C	10: 115,293,772 (GRCm39)	N408K	possibly damaging	Het
Lrp1	G	A	10: 127,396,005 (GRCm39)	H2422Y	probably damaging	Het
Mief1	T	A	15: 80,132,692 (GRCm39)	Y191*	probably null	Het
Npr3	C	G	15: 11,905,518 (GRCm39)	A70P	probably damaging	Het
Or2y1b	A	T	11: 49,208,541 (GRCm39)	H56L	probably damaging	Het
Pcnt	A	G	10: 76,265,032 (GRCm39)		probably null	Het
Pnlip	A	T	19: 58,660,174 (GRCm39)	D29V	probably benign	Het
Pnpt1	T	A	11: 29,088,567 (GRCm39)	F277I	possibly damaging	Het
Pou2f2	A	C	7: 24,793,006 (GRCm39)	V441G	probably damaging	Het
Prdm4	A	G	10: 85,740,002 (GRCm39)	V459A	probably benign	Het
Ptprs	A	T	17: 56,729,195 (GRCm39)	V855E	probably damaging	Het
Rflna	A	G	5: 125,088,352 (GRCm39)	T100A	probably benign	Het
Rnd2	C	T	11: 101,359,825 (GRCm39)	L57F	probably damaging	Het
Spata31h1	A	T	10: 82,131,871 (GRCm39)	S380T	probably benign	Het
Syn3	A	G	10: 85,893,428 (GRCm39)	S472P	unknown	Het
Taar8a	A	T	10: 23,952,674 (GRCm39)	I93F	possibly damaging	Het
Taok2	T	A	7: 126,477,930 (GRCm39)	D207V	probably damaging	Het
Tpst2	A	T	5: 112,424,600 (GRCm39)		probably benign	Homo
Trdv2-1	A	G	14: 54,183,999 (GRCm39)	N77S	possibly damaging	Het
Wdr17	A	C	8: 55,134,559 (GRCm39)	V18G	probably benign	Het
Zbtb17	T	C	4: 141,192,261 (GRCm39)	V402A	probably damaging	Het
Zw10	A	G	9: 48,980,926 (GRCm39)	D442G	probably benign	Het

Other mutations in Zfp772

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00480:Zfp772	APN	7	7,207,115 (GRCm39)	missense	probably benign
IGL01589:Zfp772	APN	7	7,208,523 (GRCm39)	missense	possibly damaging	0.53
PIT4449001:Zfp772	UTSW	7	7,207,350 (GRCm39)	missense	probably benign	0.03
R1945:Zfp772	UTSW	7	7,206,629 (GRCm39)	missense	probably benign	0.01
R3085:Zfp772	UTSW	7	7,206,699 (GRCm39)	missense	possibly damaging	0.53
R5300:Zfp772	UTSW	7	7,207,157 (GRCm39)	missense	probably benign
R5793:Zfp772	UTSW	7	7,207,283 (GRCm39)	missense	probably benign	0.00
R6252:Zfp772	UTSW	7	7,207,018 (GRCm39)	missense	possibly damaging	0.86
R6751:Zfp772	UTSW	7	7,206,716 (GRCm39)	missense	possibly damaging	0.90
R6812:Zfp772	UTSW	7	7,209,307 (GRCm39)	missense	possibly damaging	0.92
R8171:Zfp772	UTSW	7	7,207,096 (GRCm39)	nonsense	probably null
R8696:Zfp772	UTSW	7	7,208,518 (GRCm39)	missense	possibly damaging	0.53
R9104:Zfp772	UTSW	7	7,207,190 (GRCm39)	missense	possibly damaging	0.53
R9665:Zfp772	UTSW	7	7,212,793 (GRCm39)	start codon destroyed	probably null

Predicted Primers

PCR Primer
(F):5'- CATCACAATACAGGAACAGCTGTG -3'
(R):5'- TCGGAATGTAGCAGGAATTGTG -3'

Sequencing Primer
(F):5'- TACAGGAACAGCTGTGTATCAC -3'
(R):5'- AATTGTGGCACTGGTCAGC -3'

Posted On

2018-06-22